무 혈청배지에 의한 각막상피 세포의 세포고사

나명석(Myung Suk Ra),김재민(Jai Min Kim) 한국안광학회 1999 한국안광학회지 Vol.4 No.1

생육 시기별 탱자 에탄올 추출물의 항산화 및 항균 효과

전라합 ( Ra Hap Jeon ),최희진 ( Hee Jin Choi ),문승재 ( Seung Jae Moon ),나명석 ( Myung Suk Na ) 한국미용학회 2010 한국미용학회지 Vol.16 No.4

This study was to examine the effect of antioxidant and antimicrobial activity with 99% ethanol extracts of Poncirus trifoliata during growth. Antioxidant activity of Poncirus trifoliata onto free radical generation was determined by measuring DPPH and ABTS radical scavenging activities in vitro. Antimicrobial activity was tested against Escherichia coli Streptococcus aureus, Propionibaterium acnes, Malassezia furfur. Antioxidant activities of ethanol extracts of Poncirus trifoliata was 87.16% in the DPPH assay and 28.93% in ABTS assay at 2.0 mg/g. Antimicrobial activity was that Propionibaterium acnes was higher than others microorganism. These results demonstrate that unripe blue Poncirus trifoliata may be useful in the materials of cosmetics.

소프트 콘택트렌즈 용출액과 보존 용액의 세포 독성 비교

윤영(Young Yoon),나명석(Myung Suk Ra),이종빈(Jong Bin Lee),김재민(Jai Min Kim),성정섭(Jeong Sub Seong),유근창(Keun Chang Yoo) 한국안광학회 1999 한국안광학회지 Vol.4 No.2

Human corneal epithelial cells and mouse fibroblast L929 cells were gro주 to 60-70% confluency in 96 well plates. 24 hours after the well plates are inoculated, the medium on the test plates is aspirated and replaced with an extract supplemented medium prepared from the materials to be tested. The contact lenses manufactured by 7 companies were collected from optical shops and used for this study. The exactracts having been prepared by autoclaving 8 lenses in 2.5㎖ saline. The cell monolayer is then cultured for a further 48 hour period. MTT and SRB assys were performed for cytotoxic effect on cultured cells An inhibition of 30% is considered clear indication of cytotoxic potential in the test material. All the materials were not cytotoxic, but 3 storage solutions of them inhibited growing L929 cells.

광양만 미생물 군집에 관한 환경요인분석

위인선,전순배,이종빈,나명석 ( In Sun Wui,Soon Bai Chun,Jong Bin Lee,Myung Suk Ra ) 한국환경생물학회 1993 환경생물 : 환경생물학회지 Vol.11 No.1

The factor analysis was applied to examine the effects of enviromental conditions on microbial populations in Kwangyang Bay from February to December, 1990. For this purpose, 17 abiotic and biotic parameters were determined for samples obtained from fifteen sites. Ecosystem in Kwangyang bay was regulated by 5 factors for four season. The microbial population was influenced mainly by the inflow fresh water, nitrogenous nutrients and water temperature. The overall size of microbial population was greater than that obtained in 1982.

건성안의 분포와 진단 방법에 대한 연구

유근창(Geun Chang Ryu),박현주(Hyun Ju Park),김재민(Jae Min Kim),이승아(Seung A Lee),나명석(Myung Suk Ra) 한국안광학회 2000 한국안광학회지 Vol.5 No.2

To investigate the distribution of dry eye and to accept diagnosis methods of dry eye in ophthalmic shop, this study was performed on 81 persons ( male : 49, female : 32 ) . 1. We divided two group for diagnosing dry eye by dry eye screening questionnaire : one was dry eye symptom group ( 49.3% ) and the other was non - dry eye symptom group ( 50.7 % ) . 2. Non - invasive break - up time ( NIBUT) which was measured by using Tear scope R ( Killer. co)was longer than invasive break-up time(IBUT). 3. A blink rate in dry eye symptom group(10%) was measured lesser than 10 cycle/min, in non - dry eye symptom group(14.9%). 4. In shirmer test there were lesser than lOmm/5min in dry eye symptom group(67.5%), in non-dry eye symptom group(39%). 5. Cornea and conjunctival staining by rose bengal represented Mild 25%, medium 36.25%, extreme 38.75% in dry eye symptom group. Non-dry eye symptom group was showed Mild 19.51%, medium 14.63%, extreme 12.19%. 6. These results showed that the patient showed dry eye symptom in 3 items of eye examination was 22.5%, 4 items 25%. 5 items 30%. 6 items 15%.

SLS ( Sodium Lauryl Sulfate ) 가 Microcosm 내의 개체군 동태에 미치는 영향

위인선(In Sun Wui),이종빈(Jong Bin Lee),수등융일,도삼유평,조경(Kyung Cho),나명석(Myung Suk Ra),위성욱(Seong Uk Wui) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.3

We investigated each individual movement in the microcosm when synthetic detergent, SLS (sodium lauryl sulfate), was inflowed in the system. The microcosm is one of a small model ecosystem containing biotic species which selected from natural aquatic ecosystem and maintaining as culture system under controlled condition. As a result of inflowing SLS in the system 14 days after subculture (stabilizing stage), individuals of producer Chlorella vulgaris and decomposer Pseudomonas putida were stable at 10.0㎎/ℓ SLS. But, consumer Cyclidium glaucoma was begun to affect at 2.5 ㎎/ℓ SLS and this individual was disappeared in the system at 5.0 ㎎/ℓ SLS. This study was purposed for suggestion to one of environmental assessment method by using model system as investigation of individual movement in the microcosm after treating detergent, one of a aquatic pollutant.

신장 상피세포주 A498을 이용한 대황(Rheum undulatum L.) 추출물의 세포독성

나명석,진종언,조남철 한국식품영양학회 2000 韓國食品營養學會誌 Vol.13 No.5

본 연구에서는 전통적으로 담석증, 신장치료 등의 한약제로 많이 사용하는 대왕을 여러 용매를 사용하여 얻은 대왕추출물 분액에 대한 세포독성을 여부를 MTT 정량법, NR 정량법, SRB 정량법을 이용하여 조사하였다. 1. 추출 용매 methylene chloride, ethyl acetate, butanol, water로부터 얻은 대왕추출물 모두 처리농도에 따라 세포에 미치는 영향이 증가하였다. 2. Butanol을 용매로 사용하여 얻은 대왕추출물 분액이 다른 3가지 용매로부터 얻은 대왕추출물보다 세포에 미치는 영향이 크게 나타났고 water를 용매로 사용하여 얻은 추출물이 A498세포주에 미치는 영향이 가장 낮게 나타났다. 3. Butanol을 추출 용매로 하여 얻은 대왕추출물이 A498세포주에 미치는 영향이 가장 컸는데 그 추출물에 대한 MTT_50, NR_50, SRB_50값은 각각 0.63㎎/m1, 0.65㎎/m1, 0.68㎎/m1이었고, 가장 영향이 적은 water의 경우 MTT_50, NR_50, SRB_50값은 각각 0.84㎎/m1, 0.82㎎/m1, 0.80㎎/ml이었다. 4. 정량방법 간의 대왕추출물에 대한 반응은 MTT 정량법이 가장 민감하게 나타났다. We have evaluated cytotoxic effects of four crude extracts of methylene chloride, ethyl acetate, butanol, water layer isolated Rheum undulatum in A498 cell line, human kidney epithelial cells. The cytotoxic evalutation was measured by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), neutral red (NR) and sulforhodamine protein B (SRB) . These results obtained are as follows : MTT, NR and SRB quantities were significantly decreased in cultured A498 cells treated four crude extracts by increased concentrations. The cell cytotoxic effect of crude extracts of butanol layer was more stronger than others layer. The values of MTT_50, NR_50, SRB_50 of crude extract of butanol layer and were measured both 0.63 ㎎/ml, 0.65 ㎎/ml, and 0.68 ㎎/ml, respectively and the values of water layer were 0.84 ㎎/ml, 0.82 ㎎/ml, and 0.80 ㎎/ml, respectively in cultured A498 cell line.

종양의 성장 및 전이에 있어서 NF-κB의 역할

고현미,최정화,나명석,임선영 Official Journal of the Korean society for immunol 2003 Immune Network Vol.3 No.1

수계 Microcosm 을 이용한 도금폐수의 독성평가

위성욱,稻森悠平,조경,나명석,이종빈 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.3

생태계를 평가하기 위한 평가법으로 주로 사용되는 이화학적 측정 평가법은 객관적인 측정치를 제시하는데 는 용이하다. 그러나 단지 측정치의 단순한 평균이나 최고치 만으로는 타 인자와의 복합 효과 또는 지속 시간에 의한 가중 효과 때문에 실상을 나타낼 수 없는 경우가 많다. 이에 따라 이화학적 측정치들을 객관적으로 종합하여 생태계의 상태를 파악할 필요성이 보다 중요하다. 즉 key factor나 trigger factor와 같은 생물체의 내성과 관련이 있는 인자들로 종이나 생태계의 조절 기구와의 관계를 평가해야 한다. 또한 생물을 이용하여 생태계를 평가하는 방법으로 지표종을 이용한 평가방법이 있으나 오염원에 대한 누적 효과나 종합적인 평가를 판단하는데는 한계성이 있다. 따라서 생태계를 하나의 단위로 하는 평가 방법이 필요하다. 이에 따라 본 실험은 생산자, 분해자, 소비자로 구성된 microcosm 이용하여 대표적인 산업폐수인 도금공장 폐수에 대한 독성을 평가하였다. 그 결과 도금 폐수의 이화학적 성상은 크롬 2,234.8mgl^-1,구리 3.1mgl^-1,아연 521.3mgl^-1로 크롬이 다량 함유되어 있었으며, 시안은 42.9mgl^-1이었다. 도금 폐수를 농도별로 microcosm 내에 첨가한 후 각 개체군들의 동태 변화를 조사한 결과 Ch. uulgaris는 모든 처리구에서 대조구보다 감소하는 경향을 나타내었고, P putida는 대조구에 비해 증가하였으며, C.gloucoma는 농도가 증가함에 따라 개체수가 감소하기 시작하였으며, 2.5% 이상의 처리구에서는 모든 개체수가 사멸하였다. 본 연구 결과 제어된 생물환경 조건하에서 구성된 microcosm이 실험 재현성이 비교적 우수하고, 안정된 미소 생태계를 유지할 수 있어 산업 폐수 및 오폐수에 대한 새로운 독성평가 방법으로 적용이 가능할 것으로 본다. This research investigated experimentally on the population growth in the aquatic microcosm with the wastewater of plating factory. The purpose of this study was to evaluate the effect of culture conditions of the characteristic growth pattern of the examined species. Population of the system is consists of three organisms; Chlorella vulgaris as a producer, Cyclidium glaucoma as a consumer and Pseudomonas putida as a decomposer. The different growth patterns of each popultaion are followed by surfactant type; Especially C. glaucoma was sensitive, Ch. vulgaris was maintained population size stably even at high level of surfactant and P. putida was not significantly affected. After treatment of waste water from plating factory, it began to be affected at 1.0% solution treatment to Ch. vulgaris which the cell number was decreased prominently after 2 days, and C. glaucoma was disappeared at 2.5% solution treatment. P. putida was showed increasing pattern according to treatment concentration, at 2.5% solution and population size grew double. The result from current microcosm study indicates that this model system can be applied to environmental assessment method for various pollutants.

양파(Allium cepa L.) 추출물이 Tyrosinase 유전자 발현에 미치는 효과

조남철,윤연희,이혜진,손현정,김양경,최근희,나명석,조영권,이황희,진종언 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.3

Melanin 생성에 관여하는 tyrosinase 유전자의 발현을 억제하는 물질을 탐색하고자 tyrosinase promoter를 지닌 Bl6 mouse melanoma cell해 앙파 methanol 추출물을 처리한 바 양파 methanol 추풀물은 10.0㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml의 농도에서 대조군에 비해서 약 15%, 23% 57%의 억제효과를 나타냈으며 세포생존율은 1.0 ㎍/ml, 10.0 ㎍/ml, I00.0 ㎍/ml, 1.0㎎/ml의 농도에서 약 126 %, 92%. 85%, 64%로서 세포독성이 낮게 나타났다. Ethyl acetate, butyl alcohol, 그리고 물 용매 분획물은 tyrosinase 유전자의 발현물 억제하는 효과가 없었지만 methylene chloride 용해 분획물은 10.0 ㎍/ml과 100.0 ㎍/ml의 농도에서 약 37%와 35%의 발현율을 나타냄으로서 대조군에 비해 크게 억제하였다. Onion(Allium cepa L,) extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. 10.0 ㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml of the extract repressed expression of tyrosinase gene about 15%, 23%, and 57%, respectively, compared with control. In the MTT assay, the same extract exhibited low cytotoxicity at 1.0 ㎍/ml, 10.0 ㎍/ml, 100.0 ㎍/ml, and 1.0 ㎎/ml, respectively. The fractions of ethyl acetate, butyl alcohol, and water did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of methylene chloride repressed highly at 10.0 ㎍/ml and 100.0 ㎍/ml.