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촛불에서 발생하는 미세먼지 측정과 자동정화 시스템 구현
변현섭(Byeon Hyeon Seop),정연만(Jeong Yeon Man) 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.2
본 논문은 촛불에서 발생되는 미세먼지를 측정하고 미세먼지 농도에 따라 자동적으로 정화 시스템이 동작하도록 구현하였다. 최근 실내에 좋은 향기와 아늑한 분위기를 연출하기 위해 향초를 사용하고 있지만, 향초를 태운 미세먼지는 실제로 건강에 악영향을 줄뿐만 아니라 밀페된 공간에서 심해진다. 따라서 실내 공간의 내부 미세먼지 농도를 실시간적으로 측정하고, 자동 정화 시스템을 통해 미세먼지 농도를 줄이도록 연구하였다.
국내 양돈장의 사육 온도와 밀사율이 구강액 채취율에 미치는 영향
변현섭 ( Hyeon Seop Byeon ),김미화 ( Mihwa Kim ),권성애 ( Sungae Kwon ),한미나 ( Mina Han ),한성태 ( Sung Tae Han ),장래훈 ( Rae Hoon Jang ),정윤수 ( Yun-soo Chung ),김석현 ( Seokhyun Kim ),전보영 ( Bo-young Jeon ) 한국동물위생학회 2021 韓國家畜衛生學會誌 Vol.44 No.4
We investigated the effect of temperature and stock density on the collection efficiency of oral fluid in the pig farm in Korea. Three pig farms with similar breeding environmental conditions were selected and four pens of each farm (total 12 pens) were tested for the collection efficiency of oral fluid from pigs. Collection rate was considered as significant when oral fluid was collected from 70% of pigs within a pen. In the case of growing pigs, when internal temperature of pig barn increased by one designated degree (5℃), the oral fluid collection rate significantly decreased by 24.7% (P<0.05). The collection rate of oral fluid also decreased by 7.1% (P<0.05) as the density rate increase by one designated degree (12.5%). It was estimated that the collection efficiency of oral fluid decreased when the internal temperature of pig barn was 30℃ or higher, or barn density is higher 25% or high. On the other hand, in the case of stall-housing sows, unlike growing pigs, there was no significant differences according to the temperature, so oral fluid collection was considered to be efficient even in hot season.
The effect of extract from leaves and stalks of Angelica gigas on the innate immunity
Kang, Shin-Seok,Byeon, Hyeon-Seop,Kang, Shin-Kwon,Ko, Duk-Hwan,Lim, Dae-Jun,Lee, Jung-Hwa,Kang, Sung-Ho The Korean Society of Veterinary Service 2013 韓國家畜衛生學會誌 Vol.36 No.4
The dried root of Angelica gigas (A. gigas) has been traditionally used as an oriental medicine, which is known to improve blood circulation and blood stasis. In the present study, leaves and stalks of A. gigas were used to investigate their effects on the innate immunity. The extracts were prepared from leaves and stalks of A. gigas and were fed to mice. The numbers of blood cells, total WBCs, neutrophils, lymphocytes, eosinophils and basophils were increased by 50% in mice fed with leaves extract of A. gigas compared to control mice. However, the numbers of blood cells were decreased when treated with stalks extract of A. gigas. The level of cholesterol and triglyceride in serum was markedly reduced in both mice group fed with leaves extract and stalks extract of A. gigas compared to control group (P<0.01). There was no significant change in the level of albumin, total protein, phosphate and calcium in serum. Activity of cationic peptide was found to be diffused in the testicles of mice fed with leaves extract of A. gigas compared to control group, which might be due to increased lysozyme in testicle. The lysoplate assay and immunohistochemistry assay suggest that the extract of leaves and stalks of A. gigas are immunogenic, but the effects might be related with acquired immune response rather than innate immunity.
Han, Mi Na,Byeon, Hyeon Seop,Han, Seong Tae,Jang, Rae Hoon,Kim, Chang Seop,Choi, Seok Hwa The Korean Society of Veterinary Service 2018 韓國家畜衛生學會誌 Vol.41 No.4
Avian pathogenic E. coli (APEC) causes severe economic losses in the poultry farms, due to systemic infections leading to lethal colisepticemia. It causes a variety of diseases from air sac infection to systemic spread leading to septicemia. Secondary infection contains opportunistic infections due to immunosuppression disease. Collibacillosis causes the great problems in the poultry industry in Korea. Thus, it is necessary to identify and classify the characteristics of E. coli isolate of chicken origin to confirm the diversity of symptoms and whether they are transmitted among the farms. Fragment analysis is identify the difference in the number of Variable-Number Tandem-Repeats (VNTRs) for genotyping. VNTRs have repeating structure (Microsatellite, Short tandem repeats; STR, Simple sequence repeats; SSR) in the chromosome. This region can be used as a genetic marker because of its high mutation rate. And various lengths of the amplified DNA fragment cause the difference in the number of repetition of the DNA specific site. The number of repetition sequences indicates the separated size of fragments, so the each fragments can be distinguished by specific samples. The results of the sample show that there is no difference in six microsatellite loci (yjiD, aidB, molR_1, ftsZ, b1668, yibA). There are differences among the farms in relation of the number of repetitions of other six microsatellite loci (ycgW, yaiN, yiaB, mhpR, b0829, caiF). Four (ycgW, yiaB, b0829, caiF) of these six microsatellite loci show statistically significant differences (P<0.05). It means that the analysis using four microsatellite loci including ycgW, yiaB, b0829, and caiF can confirm among the farms. Five E. coli samples in one farm have same SSR repetition at all markers. But, there are significant differences from other farms at Four (ycgW, yiaB, b0829, caiF) microsatellite loci. These results emphasize again that the four microsatellite loci makes a difference in the amplified DNA fragments, enabling it to be used for E. coli genotyping.
Mi Na Han,Hyeon Seop Byeon,Cho Yeon Lee,Nam Sin Jo,Jong Hwa Lee,Rae Hoon Jang,Chang Seop Kim,Ki Jeong Na 한국동물위생학회 2019 韓國家畜衛生學會誌 Vol.42 No.4
Newcastle disease (ND) is an infectious poultry disease that caused high mortality and reduced egg production. NDVs are regularly present in the domestic duck population. And ducks play a possible role in the maintenance and transmission of NDVs. While we were monitoring the Avian Influenza, NDVs were isolated from field samples by accident. So we analysed the biological and genetic characteristics of these viruses. Lentogenic NDVs were isolated from two farms among twenty breeder duck farms. The ages of ducks were 39 weeks old in the ‘A’ farm and 3∼72 weeks old in the ‘B’ farm. And they were not inoculated with the NDVs vaccine. In the biological characteristics, the both viruses which separated from the farm ‘A’ and ‘B’ were thermostable. The amino acid sequence of a site from 112 to 119 in the fusion (F) protein was ‘GKQGRLIG’ which has monobasic motif in the samples of both farms. And this means the separated NDVs are lentogenic. Phylogenetic analysis was performed by en-tire nucleotide sequence of F protein. The virus strains from the A farm (MN095239) and the B farm (MN095240) belonged to class II genotype I. Using the analysis of whole F protein nucleic acid se-quence, the MN095239 (GenBank) had homology with Ulster strain about 99.95% and the MN095239 (GenBank) had homology with KR/CK/KU_LBM255/09 strain about 99.89%. NDV surveillance is needed to investigate epidemiological relationship of domestic breeder duck isolates in Korea.
Seroprevalence of tuberculosis in domesticated elk (<i>Cervus canadensis</i>) in Korea
Kang, Shin-Seok,Byeon, Hyeon-Seop,Ku, Bok Kyung,Kim, Sang Woo,Kim, Jiro,Woo, Jeongim,Ahn, Byeongwoo,Kim, Soojin,Monoldorova, Sezim,Park, Chan-Ho,Cho, Sang-Nae,Jeon, Bo-Young Elsevier 2016 Research in veterinary science Vol.107 No.-
<P><B>Abstract</B></P> <P>Tuberculosis is a contagious disease in animals, primarily cattle, although it also affects wild animals and humans. There are few data on the state of tuberculosis in domesticated elk (<I>Cervus canadensis</I>) in Korea. In order to investigate tuberculosis in elk, the effectiveness of an enzyme linked immunosorbent assay (ELISA) using MPB70 and MPB83 antigens was compared with the tuberculin skin test (TST), and seroprevalence was measured with this assay using serum samples collected from domesticated elk herds in Korea. The respective sensitivities of the MPB70 and MPB83 ELISAs were 51.9% (95% CI 42.0–61.6) and 49.1% (95% CI 39.3–58.9), and their specificities were 100.0% (95% CI 92.6–100.0) and 97.9% (95% CI 88.9–100.0), respectively, in comparison with the TST. The herd prevalence ranged from 50 to 80% and the mean herd seropositive rate was 67.7% (21 of 31). Of 819 serum samples, 163 (19.9%) were seropositive, and the within-region prevalence ranged from 18.5–58.0%. In conclusion, the ELISA using the MPB70 and MPB83 antigens showed moderate sensitivity and high specificity compared to TST in elk, and tuberculosis was assumed to be fairly prevalent in domesticated elk in Korea.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Establishment of ELISA using the MPB70 and MPB83 antigens for tuberculosis of elk </LI> <LI> Performance of these ELISAs (sensitivity and specificity) compared with TST </LI> <LI> Estimation of seroprevalence of tuberculosis in elk in Korea using these ELISAs </LI> <LI> First report on prevalence data of tuberculosis in domesticated elk in Korea </LI> <LI> A basic epidemiological data of tuberculosis in elk in an Asian region </LI> </UL> </P>
The effect of extract from leaves and stalks of Angelica gigas on the innate immunity
( Shin Seok Kang ),( Hyeon Seop Byeon ),( Shin Kwon Kang ),( Duk Hwan Ko ),( Dae Jun Lim ),( Jung Hwa Lee ),( Sung Ho Kang ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4
The dried root of Angelica gigas (A. gigas) has been traditionally used as an oriental medicine, which is known to improve blood circulation and blood stasis. In the present study, leaves and stalks of A. gigas were used to investigate their effects on the innate immunity. The extracts were prepared from leaves and stalks of A. gigas and were fed to mice. The numbers of blood cells, total WBCs, neutrophils, lymphocytes, eosinophils and basophils were increased by 50% in mice fed with leaves extract of A. gigas compared to control mice. However, the numbers of blood cells were decreased when treated with stalks extract of A. gigas. The level of cholesterol and triglyceride in serum was markedly reduced in both mice group fed with leaves extract and stalks extract of A. gigas compared to control group (P<0.01). There was no significant change in the level of albumin, total protein, phosphate and calcium in serum. Activity of cationic peptide was found to be diffused in the testicles of mice fed with leaves extract of A. gigas compared to control group, which might be due to increased lysozyme in testicle. The lysoplate assay and immunohistochemistry assay suggest that the extract of leaves and stalks of A. gigas are immunogenic, but the effects might be related with acquired immune response rather than innate immunity.