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Comparison of Soil Testing Methods for Plant Available Phosphate
Myung Sook Kim,Han Kang Kwak,Yoo Hak Kim,Seong Soo Kang,Myung Suk Gong,Yong Seon Zhang,Hong Bae Yoon,Chang Hoon Lee 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.3
Most test methods for plant available soil phosphate are based on the extraction with a chemical solution. The objective of this study is to evaluate available phosphate of various tests at different soil phosphate levels. Two experiments were conducted as follows: i) Extracting capacities of soil phosphate tests - Mehlich III, Mehlich II, Bray I, Olsen, Kelowna, and Modified Lancaster(Mod. Lancaster) - were compared with that of Lancaster test for the soils collected from 32 paddy and 27 upland fields with various soil chemical properties. ii) Field trials on comparing to phosphate uptake by plant were accomplished by cultivating rice and corn plants in the pots filled with the soils. Available phosphate of Lancaster test was significantly correlated with those of Mehlich III, Mehlich II, Bray I, Olsen, Kelowna, and Mod. Lancaster. In upland soils, available phosphates of all the tests were curvilinearly regressed with phosphate uptake by corn. The determination coefficients (R²) of the regression equation between available phosphate in soils and phosphate uptake by plants were ranged from 0.861 (Mehlich III) to 0.741 (Olsen). In paddy soils, the available phosphate measured by Mehlich III and Lancaster was significantly correlated with phosphate uptake by rice. In conclusion, Lancaster and Mehlich III tests could be used for predicting available phosphate in upland and paddy soils.
Comparison of Soil Testing Methods for Plant Available Phosphate
Kim, Myung Sook,Kwak, Han Kang,Kim, Yoo Hak,Kang, Seong Soo,Gong, Myung Suk,Zhang, Yong Seon,Yoon, Hong Bae,Lee, Chang Hoon 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.3
Most test methods for plant available soil phosphate are based on the extraction with a chemical solution. The objective of this study is to evaluate available phosphate of various tests at different soil phosphate levels. Two experiments were conducted as follows: i) Extracting capacities of soil phosphate tests - Mehlich III, Mehlich II, Bray I, Olsen, Kelowna, and Modified Lancaster(Mod. Lancaster) - were compared with that of Lancaster test for the soils collected from 32 paddy and 27 upland fields with various soil chemical properties. ii) Field trials on comparing to phosphate uptake by plant were accomplished by cultivating rice and corn plants in the pots filled with the soils. Available phosphate of Lancaster test was significantly correlated with those of Mehlich III, Mehlich II, Bray I, Olsen, Kelowna, and Mod. Lancaster. In upland soils, available phosphates of all the tests were curvilinearly regressed with phosphate uptake by corn. The determination coefficients ($R^2$) of the regression equation between available phosphate in soils and phosphate uptake by plants were ranged from 0.861 (Mehlich III) to 0.741 (Olsen). In paddy soils, the available phosphate measured by Mehlich III and Lancaster was significantly correlated with phosphate uptake by rice. In conclusion, Lancaster and Mehlich III tests could be used for predicting available phosphate in upland and paddy soils.
간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현
김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5
Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.
김해경,김명환,서동완,윤광희,유병무,민영일,공경엽,이재균,최재원,김연석,명승재,송병철,신은석 대한소화기내시경학회 1998 Clinical Endoscopy Vol.18 No.1
Biliary Papillomatosis is an extremely rare pathologic entity. Only about 40 cases of intrahepatic or diffuse intra- and extrahepatic papillomatosis have been described since the first report in 1959 by Caroli. Although this is a histologically benign lesion, its course is unfavaurable because of its tendency to extend to the entire biliary tract, high recurrence rate following local excision and the probability of progression to malignancy. We described herein a case of biliary papillomatosis associated with diffuse bile duct dilatation. The patient, a 59-year-old male, was admitted to our hospital because of right upper abdominal pain and weight loss. Cholangiogram revealed multiple round filling defects in common hepatic duct with intrahepatic bile duct dilatation. The patient underwent Whipple's operation with right lobectomy. Grossly, multiple small pin-head sized polypoid masses were seen in common bile duct, common hepatic duct and right intrahepatic duct. Microscapically, papillary epithelial hyperplasia with moderate cellular atypia was seen and there was no evidence of stromal invasion. He is healthy with a follow-up for 7 months.
췌관경 소견이 특징적이었던 Mucinous Ductal Ectasia 2예
김해경,김명환,이성구,유병무,민영일,박효숙,공경엽,김연석,정성애,명승재,서동완 대한소화기내시경학회 1996 Clinical Endoscopy Vol.16 No.4
We report two cases of mucinous ductal ectasia of the pancreas which showed characteristic pancreatoscopic findings. They also showed characteristic duodenoscopic findings such as patulous ampullary orifice and mucus leakage from the papilla, and underlying pathology was hyperplasia in one case and adenoma in the other case, The insertions of pancreatoscope into the main pancreatic duct were easy without previous sphincterotomy and whitish frog egg-like mucosa was noted in one case and finger-like papillary projection was noted in the other case.
Helicobacter pylori로부터 분리한 마이크로소옴의 ATPase 활성
김진호,김혜원,민영일,홍원선,명승재,양석균,정훈용,조형진,사공정,박승필 대한소화기학회 2000 대한소화기학회지 Vol.36 No.6
Background/Aims: Helicobacter pylori (H. pylori) is unique in its ability to persist in acidic environment. The main mechanisms to survive in low pH are to produce large amounts of urease and to have ATPases. This study was performed to invetigate the characteristics of ATPases of H. pylori. Methods: ATPase activity of microsomes prepared from H. pylori (ATCC 43504) was measured by enzyme-coupled assay. The effects of pH, sodium azide (NaN3), N,N-dicyclohexylcarbodiimide (DCCD), diethylstilbestrol (DES) and bafilomycin on the microsomal ATPase activities were determined. We also investigated the effect of nigericin on the ATPase activity. Results: The ATPase showed maximum activity at pH 5.5. The activitiy was decreased by 29%, 38% and 42% with the addition of NaN3, DCCD and DES, respectively. Bafilomycin had little effect on the ATPase activity. ATPase activity was decreased with the addition of DES dose-dependently, while it was increased by nigericin dose-dependently. Conclusions: These results suggest that cell membrane of H. pylori possesses two types of proton pumps, F-type and P-type ATPases, which act as H+ ATPase.