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골 조직 재생을 위한 전기수력학적 공정을 이용한 섬유구조의 PCL/ceramic 세포담체 제작
김민성(Minseong Kim),이형진(Hyeongjin Lee),전호준(HoJun Jeon),여명구(MyungGu Yeo),김용복(YongBok Kim),양지훈(Gi-Hoon Yang),김원진(WonJin Kim),김근형(GeunHyung Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
In this study, we fabricated a new ceramic fibrous scaffold, using the initial jet of an electrospinning process and ethanol media as a target. The fabricated three-dimensional (3D) fibrous ceramic structure was configured with multilayered micro-sized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes (ECMs). The fabrication of the ceramic structure was highly dependent on various processing parameters, such as the surface tension of the target media, and the flow rate and weight fraction of the polymer solution. As a tissue regenerative material, the 3D fibrous ceramic scaffold was cultured with pre-osteoblasts to observe the initial cellular activities in comparison with a solid freeform fabricated 3D scaffold sharing a similar structural geometry. The micro/nanofibrous 3D fibrous ceramic strut scaffold exhibited significantly high initial cell attachment, proliferation, and viability compared to solid freeform fabricated 3D scaffold.
플립드러닝형 대학 수업에서 사전학습과 수업참여와의 관계: 소집단 토의 발화 분석을 중심으로
김민성(Kim, Minseong),이지은(Lee, Jieun),김규은(Kim, Gyueun),김유경(Kim, Yugyeong) 한국교육심리학회 2019 敎育心理硏究 Vol.33 No.2
본 연구는 플립드러닝형 대학수업의 맥락에서 학습자의 사전학습과 수업참여와의 관계를 소집단토의활동에서 이루어지는 개별 학습자의 발화를 중심으로 살펴보았다. 사전학습의 정도는 해당 강좌에서 실시된 퀴즈점수로 측정되었으며, 소집단토의 참여도는 개별 학습자의 발화빈도와 발화량, 평균발화량, 그리고 발화내용분석에 기반한 발화유형별 발화빈도와 발화량으로 측정되었다. 먼저 학생들의 소집단토의 참여양상을 살펴본 결과, 소집단토의에서 개별 학생의 평균 발화빈도는 37.69회, 토의 전체에서 발화한 총량은 평균 286.06단어, 1회 발화당 평균발화량은 7.07단어였다. 발화빈도가 높은 발화유형은 정교화, 진행/관리, 반응, 논증/종합, 의견진술의 순이었다. 발화량에서는 정교화의 발화량이 가장 높았고, 다음은 논증/종합, 의견진술, 진행/관리, 반응의 순으로 나타났다. 사전학습정도는 발화빈도, 평균발화량과 유의미한 정적 상관관계를 나타냈으며, 의견진술, 논증/종합, 반응과 유의미한 정적상관을, 정교화와 진행/관리와는 통계적으로 유의미한 관계를 나타내지 않았다. 이러한 결과는 학습자의 사전학습정도와 소집단토의 참여빈도 및 발화내용 간에 유의미한 관련이 있다는 것을 보여준다. 본 연구의 결과는 소집단토의에서 학습자의 참여를 촉진하고 소집단토의가 수업내용에 기반하여 충실하게 이루어지기 위해서는 사전학습에 대한 효과적인 설계가 필요하다는 점을 시사한다. The purpose of this study was to investigate the relationship between pre-class learning and learners" participation in the context of a flipped classroom in a college. The degree of pre-class learning was measured by the average quiz scores of the learners. The individual learner"s participation in the small group discussion was calculated by the learner"s utterance frequency, utterance length in words, and the average utterance length in words. The mean frequency of each learner"s utterance was 37.69. The total length of utterance was 286.06 words, and the mean length of utterance per utterance was 7.07 words. Categories were identified based on content analysis of the utterances. The categories from the highest to lowest frequency were elaboration, leading/management, response, rationale/synthesis, and opinion presentation. In terms of the length of utterance, elaboration was the highest, followed by rationale/synthesis, opinion presentation, leading/management, and response. The degree of pre-class learning showed a statistically significant positive correlation with the frequency of utterances and the average length of utterances. More specifically, the degree of pre-class learning was positively correlated with opinion presentation, rationale/synthesis, and response. These findings indicate that the degree of pre-class learning is significantly related to learners" frequency of participation, as well as to the content of the utterances in small group discussions. The results of this study suggest that effective design for pre-class learning in a flipped classroom is critical to facilitate learners" participation in small group discussions and to ensure quality of learning activities.
WonJin Kim(김원진),JiUn Lee(이지운),Gi-Hoon Yang(양지훈),Hyeongjin Lee(이형진),YongBok Kim(김용복),Minseong Kim(김민성),YoungWon Koo(구영원),GeunHyung Kim(김근형) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
Collagen- based cell-printing technology has provided a new strategy for tissue engineering. However, although collagen-based scaffolds can provide an outstanding biofunctional benefits, their low mechanical strength and poor controllability have been limitation for their usage as hard tissue regeneration. To overcome this limitation, α-tricalcium phosphate (α-TCP) has been used for biomedical scaffolds. α-TCP is biocompatible and soluble material, and hydrolyzed rapidly to calcium-deficient hydroxyapatite which makes α-TCP a useful material for bone tissue regeneration. in this study, we fabricate 3-dimensional (3D) scaffold based on α-TCP/collagen struts coated with collagen-based bioink. To compare the physical and cellular activities, we used a scaffold composed of α-TCP/collagen scaffold coated with cell-embedded collagen. Following fabrication of the cell (MC3T3-E1)-embedded a-TCP/collagen scaffold, the cellular activities were evaluated in vitro.
김예슬(YeSeul Kim),김민성(Minseong Kim),전호준(Hojun Jeon),김용복(YongBok Kim),이형진(Hyeongjin Lee),황헌(Heon Hwang),조재열(JaeYoul Cho),김근형(GeunHyung Kim) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
The inherent properties of nanosized silica (sil), such as high biocompatibility, chemical and colloidal stability, and easy surface modification, have provided silica materials with a tremendous potential in biomedical applications. In this study, the biocomposites consisting of poly (ε-caprolactone) (PCL) and Sil fabricated by a melt-plotting/coating process can be applied as a potential scaffold for bone tissue regeneration. The pore size and strut diameter of the multi-layered biocomposites were fixed at approximately 300㎛ and 300㎛, respectively, and the morphology, hydrophilic properties, water-absorption, and mechanical strength of various compositions (1.8, 4.8, 9.4wt% of sil) in the composites were evaluated. Through the water-contact angle and water-absorption, the bio-composites displayed dramatically increased hydrophilic properties, and highly roughened surface compared to the pure PCL scaffold. The in vitro biocompatibilities (cell proliferation and mineralization) of the bio-composites wereexamined using pre-osteoblasts (MC3T3E1). Based on scanning electron microscope images, the cells were more easily adhered and grown on the surface of the bio-composites, showing enhanced mineral deposition compared to the pure PCL scaffold after 14 days of cell culture. These results were because the coated sil component in the bio-composites could induce the osteogensis of the composites. Based on the physical and biological activities, we believe that the biocomposite will be a potential biomaterial for enhancing bone tissue regeneration.
Phosphorylation by NLK inhibits YAP ‐14‐3‐3‐interactions and induces its nuclear localization
Moon, Sungho,Kim, Wantae,Kim, Soyoung,Kim, Youngeun,Song, Yonghee,Bilousov, Oleksii,Kim, Jiyoung,Lee, Taebok,Cha, Boksik,Kim, Minseong,Kim, Hanjun,Katanaev, Vladimir L,Jho, Eek‐,hoon unknown 2017 EMBO reports Vol.18 No.1
<P>Hippo signaling controls organ size by regulating cell proliferation and apoptosis. Yes-associated protein (YAP) is a key downstream effector of Hippo signaling, and LATS-mediated phosphorylation of YAP at Ser127 inhibits its nuclear localization and transcriptional activity. Here, we report that Nemo-like kinase (NLK) phosphory-lates YAP at Ser128 both in vitro and in vivo, which blocks interaction with 14-3-3 and enhances its nuclear localization. Depletion of NLK increases YAP phosphorylation at Ser127 and reduces YAP-mediated reporter activity. These results suggest that YAP phosphorylation at Ser128 and at Ser127 may be mutually exclusive. We also find that with the increase in cell density, nuclear localization and the level of NLK are reduced, resulting in reduction in YAP phosphorylation at Ser128. Furthermore, knockdown of Nemo (the Drosophila NLK) in fruit fly wing imaginal discs results in reduced expression of the Yorkie (the Drosophila YAP) target genes expanded and DIAP1, while Nemo overexpression reciprocally increased the expression. Overall, our data suggest that NLK/Nemo acts as an endogenous regulator of Hippo signaling by controlling nuclear localization and activity of YAP/Yorkie.</P>