몇가지 植物의 棲息地 造成에 關한 硏究

윤종선,김익환,신세균,심우경,이철희,신영철 韓國植物ㆍ人間ㆍ環境學會 1999 인간식물환경학회지 Vol.2 No.2

종의 棲息密度가 낮아지고 있는 하늘나리, 섬초롱, 자란 3종의 식물을 인위적으로 棲息地를 造成하기 위하여 국립공원 속리산 등산로변 標高 200m, 400m, 600m 지점에 150주식 종당 450주를 조직배양 및 분주에 의하여 육묘 된 묘를 이식하여 생존상태 및 棲息地 造成의 가능성을 2년에 걸쳐 검토한 결과는 다음과 같다. 1. 造成 棲息地의 토양의 理化學的 造成은 pH 5.2∼5.6, 유기물 함량 29∼54g/㎏, CEC 9.4∼11.5㎝ mol(+)/㎏으로 산성을 띠고 있었으며, 유기물 함량은 높은 편이었고, 염기치환용량은 낮았으나 비교적 우리나라 典型的 山地土壤의 理化學的 造成과 유사하였다. 2. 棲息地內 생장기간의 投光率은 標高 200m 지역은 10.8∼11.6%, 400m lw역은 10.8∼11.4%, 600m 지역은 11.0∼12.8%로 식재식물의 生長環境에 부족한 상태였다. 3. 식재 식물 수는 식재 후 활착율은 3종 모두 양호하였으나, 점차 감소되었으며, 특히 자란의 감소율이 높았다. 감소의 원인을 분석한 결과 하늘나리, 섬초롱, 자란 모두 굴취나 절취의 피해가 많았으며, 특히 자란은 凍害에 의한 枯死가 1년차 72∼86주, 2년 차에서 19∼35주로 조사도어 자연상태에서의 越冬은 어려운 것으로 나타났으며, 집중호우에 의한 流失도 감소의 한 원인이었다. 4. 식재식물의 생장상태는 불량하였는데 이는 投光量 부족으로 판단되어 棲息地造成時에는 錄陰期 投光量에 대한 사전 검토가 중요한 요인으로 나타났다. 5. 개화율은 섬초롱과 자란에서 높았으며 標高가 높을수록 개화기는 2∼4일 늦어지고 휴면기는 빨라지는 경향이었다. 식물 棲息地 造成時에는 造成 식물의 생장에 적합한 토양, 光環境 등을 고려한 위치 선정이 중요하며, 上層林에 의한 遮光으로 下層 초본식물 遷移速度가 빨라짐으로써 光環境 개선을 위한 上層林의 間伐, 周邊樹種의 更新이 요구된다. In order to make the artificial habitats of Lillium concolor, Campanula takesimana and Bletilla striata that the number of plant became sparse, 150 plants per species were transplanted at the region of 200m, 400m and 600m above sea level. And the growth of plants and the potential as habitats creation were analyzed. In the physical and chemical properties of soils in the there, soil pH was 5.2 to 5.6, organic matter content was 29 to 54g/㎏ and cation exchange capacity(CEC) was 9.4 to 11.5㎝ mol(+)/㎏. The transmittance rates of sunlight of the habitats during the growing periods were 10.8 to 11.6% in the 200m region, 10.8 to 11.4% in the 400m region and 11.0 to 12.8% in the 600m region. These transmittance rates were deficient for normal growth of plants. After the plants were transplanted, the survival rates of three species were high in common but the rates decreased gradually. Especially the decrease rate was high in the Bletilla striata. The causes of decrease were digging and cutting by climbers, sweeping asay by rainfall and freezing damage. Especially, the cold tolerance of Bletilla striata was weak in the natural condition. The growth of planted plants was poor, and it was assumed that the reason was shortage of sunlight transmittance. The flowering rates were high in the Campanula takesimana and Bletilla striata. And as the elevations were high, the flowering was later by 2 to 5 days but the dormancy was earlier. In the artificial making of plant habitats, the growth environment of plants have to be considered to choose the suitable region. And, because the habitats are shaded by the trees and bushes, the thinning of trees and the change of species are needs to improve the light transmission.

野生物棲息環境을 위한 人工浮島開發

심우경,이광우,이동익 고려대학교 자연자원연구소 2000 自然資源科學硏究 Vol.8 No.-

Decursinol angelate and Decursin purified from the Root of angelica gigas Nakai are both Cytotoxic Agents and Protein Kinase C Activators

Kyung Seop Ahn,In Kuk Lee,Young Bae Seu,Woong Seop Sim,Ik Hwan Kim 생화학분자생물학회 1996 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

면역증강성 다당 생산을 위한 참당귀 세포배양의 최적조건

안경섭,서원택,심웅섭,김익환 한국산업미생물학회 1998 한국미생물·생명공학회지 Vol.26 No.2

참당귀 세포(Angelica gigas H4)를 현탁배양하여 면역증강성 다당을 생산하기 위한 최적배지 설계 및 생산조건 최적화 연구를 수행하였다. 우선 세포의 성장에 가장 좋은 배지로서 Schenk and Hildebrandt(SH) 배지를 선택하여 배양한 결과 25℃, 암배양조건에서 각각 15.8 g DCW/ℓ와 0.85 g/ℓ의 세포 및 다당을 얻을 수 있었다. 한편 다당 생산용 배지의 개발을 위하여 여러 가지 식물세포배양용 배지중에서 다당생산능이 가장 높은 배지로서 Gamborg B5 배지를 선정하였으며 B5 배지를 변형하여 면역증강성 다당생산을 위한 최적배지(PPM)를 개발하였다. 그리고 최적배지를 이용한 당귀 세포증식과 다당 생산을 위한 최적 pH는 6.0-6.5, 배양온도는 20℃, 그리고 광조건보다는 암조건이 좋은 것으로 밝혀졌다. 상기 최적조건에서의 최대 세포증식과 다당생산을 각각 14.8 g/ℓ, 1.5 g/ℓ이었다. An Immunostimulating polysaccharide was produced from the suspension culture of Angelica gigas H4 plant cells. In order to enhance the polysaccharide production by the A. gigas cell culture, medium composition and physical conditions were optimized. Schenk and Hildebrandt (SH) medium was selected as an optimal basal medium for the growth of A. gigas. The maximum cell and polysaccharide concentration obtained in SH medium were 15.8 g DCW/ℓ and 0.85 g polysaccharide/ℓ, respectively, at 25℃ under dark condition. For the enhanced polysaccharide production, a polysaccharide production medium (PPM) was established by modifying Gamborg B5 medium with optimized carbon sources, growth regulators, organic and inorganic elements. Optimal initial pH and temperature were 6.0-6.6 and 20℃, respectively, and the dark condition was better than the light condition. The maximum polysaccharide concentration of 1.5 g/ℓ could be obtained through the optimization of the medium composition and physical conditions.

경기 북부 지역 학교 급식 종사원의 직무 만족도에 관한 연구

민경찬 ( Kyung Chan Min ),이명호 ( Myung Ho Lee ),박혜원 ( Hae Won Park ),박영심 ( Young Sim Park ),신용칠 ( Yong Chill Shin ),조규봉 ( Gyu Bong Cho ),이경익 ( Kyoung Ik Rhie ),정광옥 ( Koang Ock Jeaung ),신임숙 ( Yim Sook Shin 한국식품영양학회 2006 韓國食品營養學會誌 Vol.19 No.2

만성 간질환에서 Tissue Polypeptide Specific Antigen의 진단적 가치

김경수,박창영,이만호,최권,이상종,김병익,정을순,이승세,전경홍,유종현,심성춘,최윤상 대한소화기학회 1998 대한소화기학회지 Vol.30 No.5

Detection of Anticancer Activity from the Root of Angelica gigas in Vitro

AHN, KYUNG SEOP,SIM, WOONG SEOP,KIM, IK HWAN 한국미생물 · 생명공학회 1995 Journal of microbiology and biotechnology Vol.5 No.2

Anticancer activity of a fraction of the ethanol extract from the root of Korean angelica (Angelica gigas Nakai) was recognized in human cancer cell lines HeLa S_3 K-562, and Hep G_2. The extract blocked the phorbol ester-inducing megakaryocytic differentiation of K-562 cells, which indicated the modification of protein kinase C (PKC) activity. In vitro assay showed the activation of PKC by the extract. An effective fraction of the Angelica gigas extract, of which R_f value was 0.64 in a thin layer chromatography, was a different component from those of European angelicas. The ED_50 value of the fraction was 8, 9, and 16 ㎍/㎖ against HeLa S_3 Hep G_2 and K-562 cells, respectively, while the fraction showed higher ED_50 values against normal cell lines.

분리형 기관지 Blocker 에 관한 연구

양미경,정익수,곽미숙,김갑수,심우석,함태수 대한마취과학회 2001 Korean Journal of Anesthesiology Vol.40 No.5

Background: Compared with blockers mounted on the endotracheal tube, (a conjugated blocker), studies about blockers that are independent of a single-lumen tube, (a separated blocker) are rare. This study's object was to find several characteristics about these new blokers. Methods: We compared the peak and plateau airway pressures in intubated patients (n = 30) with, (group EB) and without, (group E) blockers. We measured the depth of the blockers and the pressures of the balloon of the blockers. Balloon pressures were measured twice outside and inside of the E-tube with 7 ml of air. All of these measurements were done in the supine position with 1 L/min flow rate. The frequency of malposition, the score of the lung collapse, and bronchial mucosa change were checked thereafter. Results: The peak airway pressures of group EB were significantly higher than those of group E (P<0.05). However, the plateau pressures of both groups were not different. The depth of the blockers was 52.0±1.8 cm, and the balloon pressures of the blockers were 124±22 and 139±20 mmHg, respectively. Six patients needed additional bronchoscopy because of malposition. The lung collapse score was good in 17 patients, fiar in 10 patients, and poor in 3 patients. There was not any severe bronchial mucosa damage. Conclusions: Understanding the characteristics of these new blockers, we can consider the use of them in some situations in which the use of a double-lumen tube is impossible or inconvenient.

흑찰거대배아미를 이용한 팝라이스와 흑미차의 품질 특성

한상익(Sang-Ik Han),서우덕(Woo Duck Seo),나지은,박지영(Ji-Young Park),박동수(Dong-Soo Park),조준현(Jun-Hyun Cho),이종희(Jong-Hee Lee),서경혜(Kyung-Hye Seo),심은영(Eun-Yeong Sim),남민희(Min-Hee Nam) 한국생명과학회 2015 생명과학회지 Vol.25 No.1

거대쌀눈의 특성과 검정쌀 그리고 찹쌀의 특성을 보유한 흑찰거대배아미인 ‘눈큰흑찰’을 이용한 팝라이스 제조와 이를 이용한 현미차로 이용하였을 경우의 주요 성분의 분석 결과, 눈큰흑찰을 이용한 최적의 팝라이스 가공조건은 157℃에서 5 ~ 20분 볶는 조건이 상품성이 가장 적당함을 확인하였다. 눈큰흑찰 팝라이스는 대조인 일미에 비해 단백질(11.3%)과 지방(3.7%)이 높음을 보였고, 당 함량과 조성에서도 일미에 비해 다량 함유함을 보였다. 멜라노이딘 색소 함량에서 일미에 비해 4배(0.2 OD) 증가하였고, 폴리페놀, 가바(GABA), 총 아미노산 등 주요 영양과 기능성분에서 가공에 의한 눈큰흑찰의 특성이 좋음을 확인하였다. 현미차로 눈큰흑찰 팝라이스를 우려내었을 경우도 글루코오스, 슈크로오스, 라피노오스, 총 당함량에서 현미와 일미에 비해 다량 용출됨을 보였고, 특히 당도와 가장 밀접한 관계가 있는 슈크로오스는 현미에 비해 4배 이상 증가함을 보였다. 항산화성 분석을 통한 생리활성 검정에서도 눈큰흑찰 팝라이스는 현미에 비해 4배(DPPH, SC50, 0.24 mg/ml) 이상의 항산화력이 있음을 보여주었다. Recently developed black waxy rice with a giant embryo (‘Nunkeunheukchal’, BGE) was selected and processed to produce high quality nutritional food. BGE contains high levels of several phytochemicals with antioxidant activities, as well as other reported health beneficial properties. In addition, the giant embryo has high protein, lipid, and amino acids contents. Within the free amino acids, γ-aminobutyric acid (GABA), a major inhibitory neurotransmitter, has long been used for treating the aftereffects of brain injuries and stroke. A method for manufacturing pop-rice and black rice tea by popping process in BGE is provided to increase a taste, nutrition and functionality. The produced ‘pop-rice’ showed increased protein (11.3%) and lipid (3.7%) contents compared with control variety, IB (‘Ilmibyeo’). In addition, melanoidin related products, polyphenol and functional amino acid contents were increased by the popping process. Pop-rice tea made of BGE showed the highest extraction of total sugar, glucose, raffinose and sucrose (4 times higher than brown rice) by hot water. Scavenging activity (SC50) of processed BGE rice powder showed strong antioxidative activity of 0.24 mg/ml using DPPH and 1.82 mg/ml using ABTs method. Thereafter, these results suggested that the popping processed rice of BGE could be one of the promising materials for healthy food development.

Terahertz Spectroscopy of Magnons in HoFeO₃ and Detection of Rabi Splitting

Howon Lee,Kyung Ik Sim,Hyunjun Shin,Y. J. Choi,Jae Hoon Kim 한국자기학회 2020 한국자기학회 학술연구발표회 논문개요집 Vol.30 No.2