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WonJin Kim(김원진),JiUn Lee(이지운),Gi-Hoon Yang(양지훈),Hyeongjin Lee(이형진),YongBok Kim(김용복),Minseong Kim(김민성),YoungWon Koo(구영원),GeunHyung Kim(김근형) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
Collagen- based cell-printing technology has provided a new strategy for tissue engineering. However, although collagen-based scaffolds can provide an outstanding biofunctional benefits, their low mechanical strength and poor controllability have been limitation for their usage as hard tissue regeneration. To overcome this limitation, α-tricalcium phosphate (α-TCP) has been used for biomedical scaffolds. α-TCP is biocompatible and soluble material, and hydrolyzed rapidly to calcium-deficient hydroxyapatite which makes α-TCP a useful material for bone tissue regeneration. in this study, we fabricate 3-dimensional (3D) scaffold based on α-TCP/collagen struts coated with collagen-based bioink. To compare the physical and cellular activities, we used a scaffold composed of α-TCP/collagen scaffold coated with cell-embedded collagen. Following fabrication of the cell (MC3T3-E1)-embedded a-TCP/collagen scaffold, the cellular activities were evaluated in vitro.
김예슬(YeSeul Kim),김민성(Minseong Kim),전호준(Hojun Jeon),김용복(YongBok Kim),이형진(Hyeongjin Lee),황헌(Heon Hwang),조재열(JaeYoul Cho),김근형(GeunHyung Kim) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
The inherent properties of nanosized silica (sil), such as high biocompatibility, chemical and colloidal stability, and easy surface modification, have provided silica materials with a tremendous potential in biomedical applications. In this study, the biocomposites consisting of poly (ε-caprolactone) (PCL) and Sil fabricated by a melt-plotting/coating process can be applied as a potential scaffold for bone tissue regeneration. The pore size and strut diameter of the multi-layered biocomposites were fixed at approximately 300㎛ and 300㎛, respectively, and the morphology, hydrophilic properties, water-absorption, and mechanical strength of various compositions (1.8, 4.8, 9.4wt% of sil) in the composites were evaluated. Through the water-contact angle and water-absorption, the bio-composites displayed dramatically increased hydrophilic properties, and highly roughened surface compared to the pure PCL scaffold. The in vitro biocompatibilities (cell proliferation and mineralization) of the bio-composites wereexamined using pre-osteoblasts (MC3T3E1). Based on scanning electron microscope images, the cells were more easily adhered and grown on the surface of the bio-composites, showing enhanced mineral deposition compared to the pure PCL scaffold after 14 days of cell culture. These results were because the coated sil component in the bio-composites could induce the osteogensis of the composites. Based on the physical and biological activities, we believe that the biocomposite will be a potential biomaterial for enhancing bone tissue regeneration.
골 조직 재생을 위한 전기수력학적 공정을 이용한 섬유구조의 PCL/ceramic 세포담체 제작
김민성(Minseong Kim),이형진(Hyeongjin Lee),전호준(HoJun Jeon),여명구(MyungGu Yeo),김용복(YongBok Kim),양지훈(Gi-Hoon Yang),김원진(WonJin Kim),김근형(GeunHyung Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
In this study, we fabricated a new ceramic fibrous scaffold, using the initial jet of an electrospinning process and ethanol media as a target. The fabricated three-dimensional (3D) fibrous ceramic structure was configured with multilayered micro-sized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes (ECMs). The fabrication of the ceramic structure was highly dependent on various processing parameters, such as the surface tension of the target media, and the flow rate and weight fraction of the polymer solution. As a tissue regenerative material, the 3D fibrous ceramic scaffold was cultured with pre-osteoblasts to observe the initial cellular activities in comparison with a solid freeform fabricated 3D scaffold sharing a similar structural geometry. The micro/nanofibrous 3D fibrous ceramic strut scaffold exhibited significantly high initial cell attachment, proliferation, and viability compared to solid freeform fabricated 3D scaffold.
Kim, WonJin,Lee, Hyeongjin,Kim, YongBok,Choi, Chang Hyun,Lee, DaeWeon,Hwang, Heon,Kim, GeunHyung IOP Publishing 2016 Biomedical materials Vol.11 No.5
<P>In recent years, a variety of biomimetic hydrogel scaffolds have been used in tissue engineering because hydrogels can provide reasonable soft-tissue-like environmental conditions for various cell responses. However, although hydrogels can provide an outstanding biofunctional platform, their poor mechanical stability and low processability have been obstacles for their usage as biomedical scaffolds. To overcome this limitation, we propose a simple and versatile method using 3D printing supplemented with a low-temperature working plate and coating process to reinforce the mechanical properties and various cellular activities by accommodating the poly(epsilon-caprolactone) (PCL). To determine the efficiency of the method, we used two typical hydrogels (alginate and collagen), which were deposited in a multi-layer configuration, and PCL as a coating agent. The scaffolds were evaluated in terms of various physical and cellular activities (metabolic activity and osteogenic activity). Throughout the experiments, significant increases in the tensile modulus (> 6-fold), cell proliferation (> 1.2-fold), and calcium deposition (> 1.3-fold) were observed for the hydrogel/PCL scaffolds compared to those for pure hydrogel. Based on the experimental results, we can confirm that the proposed hydrogel scaffold can be a highly promising biomedical scaffold for application in tissue regeneration.</P>
Kim, WonJin,Jang, Chul Ho,Kim, GeunHyung Elsevier 2017 Materials Science and Engineering C Vol. No.
<P>Collagen has been widely used as a very promising material to regenerate various tissues. It is a chief component of the extracellular matrix, and encourages various biological effects conducive to tissue regeneration. However, poor mechanical stability, low processability, and high level of water absorption can lead to impaired control of growth factor release and have impeded the use of collagen as a functional biomedical scaffold. Here, to overcome the shortcomings of collagen scaffolds, we have additively manufactured collagen/polycaprolactone (PCL) biocomposites supplemented with a bioceramic (hydroxyapatite (HA)/(beta-tricalcium-phosphate (TCP)) and two growth factors (recombinant human bone morphogenetic protein-2 [rhBMP-2] and platelet-rich plasma [PRP]). Various weight fractions of PCL in the collagen/PCL composites were manipulated to select optimal growth factor release and highly active cellular responses. After the optimal concentration of PCL in the collagen/PCL scaffold was determined, biocomposites supplemented with bioceramic/growth-factors were fabricated. Continuously released growth factors were assumed to increase the in vitro cellular activities of the osteoblast-like cells (MG63) cultured on the biocomposites. In vitro cellular responses, including osteogenic activities, were examined, and results showed that compared to the HA/TCP/rhBMP-2 supplemented scaffold the HA/TCP/PRP biocomposites provide significantly high cellular activities (cell proliferation: >1.3-fold) and mineralization (calcium deposition: >1.4-fold, osteocalcin: >2.6-fold) sufficient for regenerating bone tissue. (C) 2017 Published by Elsevier B.V.</P>
Kim, Haeri,Yang, Gi Hoon,Kim, GeunHyung Elsevier 2019 International journal of biological macromolecules Vol.135 No.-
<P><B>Abstract</B></P> <P>The surface topography of a tissue-engineered scaffold is widely known to play an essential role in bone tissue engineering applications. Therefore, the cell-to-material interaction should be considered when developing scaffolds for bone tissue regeneration. Bone is a dynamic tissue with a distinct hierarchical structure composed of mostly collagen and bioceramics. In this study, the surface of gelatin/PVA scaffold (CF-G5P5) coated with fibrillated collagen was fabricated to enhance cell proliferation and osteogenic differentiation for bone tissue regeneration. The physical and biological properties of the fabricated scaffolds were investigated. As a result, the CF-G5P5 scaffold increased surface roughness and increased protein absorption compared to a gelatin/PVA scaffold (G5P5) by 1.6 times from OD value 0.43 to 0.71 after 12 h, cell proliferation increased 1.7 times from OD value 0.57 to 0.96, and differentiation increased by 1.5 times from 100 to 151%. Based on the results, the CF-G5P5 scaffold developed can be considered as a highly potential bone tissue regenerative material.</P>