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Lee, JiUn,Yeo, Miji,Kim, WonJin,Koo, YoungWon,Kim, Geun Hyung Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.110 No.-
<P><B>Abstract</B></P> <P>Cell-printing is an emerging technique that enables to build a customized structure using biomaterials and living cells for various biomedical applications. In many biomaterials, alginate has been widely used for rapid gelation, low cost, and relatively high processability. However, biocompatibilities enhancing cell adhesion and proliferation were limited, so that, to overcome this problem, an outstanding alternative, collagen, has been extensively investigated. Many factors remain to be proven for cell-printing applications, such as printability, physical sustainability after printing, and applicability of <I>in vitro</I> cell culture. This study proposes a cell-laden collagen scaffold fabricated <I>via</I> cell-printing and tannic acid (TA) crosslinking process. The effects of the crosslinking agent (0–3wt% TA) in the cell-laden collagen scaffolds on physical properties and cellular activities using preosteoblasts (MC3T3-E1) were presented. Compared to the cell-laden collagen scaffold without TA crosslinking, the scaffold with TA crosslinking was significantly enhanced in mechanical properties, while reasonable cellular activities were observed. Concisely, this study introduces the possibility of a cell-printing process using collagen and TA crosslinking and <I>in vitro</I> cell culture for tissue regeneration.</P>
중금속 오염 토양에서 분리한 니켈 내성 균주의 동정과 유전체 분석을 통한 잠재적 기작 연구
이슬 ( Seul Lee ),( Anamika Khanal ),여준구 ( Junkoo Yeo ),( Kathyleen Nogrado ),조아현 ( Ahyeon Cho ),송윤진 ( Yoonjin Song ),권미지 ( Miji Kwon ),이지훈 ( Ji-hoon Lee ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-
The widespread use of metals influenced many researchers to examine the relationship between heavy metal toxicity and bacterial resistance. In this study, we have inoculated heavy metal contaminated soil from Jang-hang region of South Korea in the nickel-containing media (20 mM Ni) for the enrichment. Among dozens of the colonies acquired from the several transfers and serial dilutions with the same concentrations of Ni, 3 colonies with morphological differences were chosen for further studies, which were named as strains Ni-a, Ni-2 and Ni-3. The isolates were identified for their phylogenetic affiliations by using 16S rRNA gene analysis. The strains Ni-2 and Ni-3 were close to Cupriavidus metallidurans and were found to be resistant to antibiotics of vancomycin, erythromycin, chloramphenicol, ampicillin, gentamicin, streptomycin and kanamycin by disk diffusion method. Of the isolated strains, Ni-2 was sequenced for the whole genome, since the Ni-resistance seemed to be better than the other two strains. From the genome sequence we have found that were a total of 89 metal-resistance-related genes including 11 Ni-resistance genes, 41 heavy metal (As, Cd, Zn, Hg, Cu, and Co)-resistance genes, 22 cation efflux genes, 4 metal pumping ATPase genes, and 11 metal transporter genes.
Huh, JunTae,Lee, JiUn,Kim, WonJin,Yeo, Miji,Kim, GeunHyung Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.110 No.-
<P><B>Abstract</B></P> <P>In this study, we suggest a new biocomposite scaffold composed of gelatin/α-TCP (tricalcium phosphate)/SF (silk-fibroin) (GTS) which has enhanced mechanical strength and high level of cellular activity. To fabricate GTS scaffold, a temperature-controlled 3D printing process was used and appropriate printing conditions were selected based on rheological data. To show the feasibility as a biomedical scaffold for bone tissue regeneration, the various physical and biological results, using MG63 (osteoblast-like cells), of the GTS scaffold were compared with those of a pure gelatin (G) and gelatin/α-TCP (GT) composite scaffold. GTS scaffolds showed enhanced mechanical properties in dry and wet state compared to those of the G and GT scaffolds. Also, significantly high cell-proliferation and differentiation of MG63 cells were observed in the GTS scaffold. Therefore, the GTS composite scaffold will be one of highly potential biomaterials to be used in bone regeneration.</P>