Fumaric acid와 mild heat의 병합 처리에 따른 시금치의 저장 중 미생물 제어 효과

손현정 ( Hyeon Jeong Son ),강지훈 ( Ji Hoon Kang ),오덕환 ( Deog Hwan Oh ),민세철 ( Sea Cheol Min ),송경빈 ( Kyung Bin Song ) 한국응용생명화학회 2016 Journal of Applied Biological Chemistry (J. Appl. Vol.59 No.1

시금치에 fumaric acid와 mild heat의 병합처리를 통해 병원성 미생물 제어효과를 규명하고자 시금치에 E. coli O157:H7, L. mon℃ytogenes 를 접종한 후 각 단일처리 후 미생물 수 변화를 측정하였다. Fumaric acid (0.1, 0.3, 0.5%)와 mild heat (40, 50, 60℃)의 각 단일처리 실험 결과를 토대로, 병합처리를 위한 fumaric acid의 최적농도는 0.5%, mild heat 처리조건으로 50℃ 에서 5 min으로 선정하였고, 병합처리 시 L. mon℃ytogenes, E. coli O157:H7의 수는 대조구에 비해 각각 2.53, 2.62 log CFU/g 감소하였다. 그리고 신선한 시금치에 병합처리 후 4℃에서 12일간 저장하면서 미생물 수 감소 및 품질 변화를 조사하였다. 시금치의 초기 미생물 수에 있어서 대조구와 비교하여, 병합 처리구에서 총 호기성 균을 2.77 log CFU/g 감소시켰다. 특히, 저장 12일 후 병합 처리구의 총 호기성 균 수는 4.84 log CFU/g으로 대조구와 비교하여 1.82 log CFU/g의 감균 효과를 가졌다. 또한 시금치의 저장 중 Hunter 색도 값 및 비타민 C 함량에 있어서 처리구 간의 유의적인 차이를 보이지 않았다. 따라서 본 연구 결과, fumaric acid와 mild heat의 병합처리가 시금치의 미생물학적 안전성 유지에 효과적인 처리라고 판단된다. The objective of this study was to examine the combined effect of fumaric acid with mild heat on the inactivation of microorganisms on spinach. Spinach leaves were in℃ulated with Escherichia coli O157:H7 and Listeria mon℃ytogenes. Based on the results of single treatment of fumaric acid (0.1, 0.3, and 0.5%) or mild heat (40, 50, and 60℃) regarding the inactivation of the in℃ulated bacteria, the optimal condition for the combined treatment was suggested to be 0.5% fumaric acid and mild heat treatment at 50℃ for 5 min. The combined treatment of fumaric acid with mild heat caused 2.53 and 2.62 log reductions of the populations of L. mon℃ytogenes and E. coli O157:H7, respectively. In addition, during storage of fresh spinach at 4℃ for 12 d, the combined treatment reduced initially the populations of total aerobic bacteria by 2.77 log CFU/g compared with the control. In particular, after 12 d of storage, the population of total aerobic bacteria for the combined treatment sample was 4.84 log CFU/g, whereas the control sample had 6.66 log CFU/g. Color and vitamin C content of spinach samples were not altered significantly by the combined treatment during storage. These results indicate that the combined treatment of fumaric acid with mild heat is an effective method to control microorganisms on spinach during storage.

분변토를 이용한 VOCs 흡착에 관한 기초연구

김재홍,손희정,김장호,김수생,서정민 동아대학교 환경문제연구소 1997 硏究報告 Vol.20 No.2

This study was carried out two point view that reuse of sludge and adsorption of benzene, toluene and o-xylene of VOCs in cast, carbonized cast and activated carbon. The cation exchange capacity of cast and carbonized cast were 59.2, 112 meq/l00g, respectively. The specific surface were 560, 800 ㎡/g, respectively. The average removal rates of benzene by 50g cast of 50% hydrous cast, anhydrous cast, carbonized cast, activated carbon were 15.0, 41.2, 88.2, 99.4% in 60min retention time. The average removal rates of toluene by 50g cast of 50% hydrous cast, anhydrous cast, carbonized cast, activated carbon were 12.5, 34.2, 88.2, 99.5% in 60min retention time. The average removal rates of o-xylene 50g cast of 50% hydrous cast, anhydrous cast, carbonized cast, activated carbon were 8.8, 28.5, 84.8, 98.1% in 60min retention time. The adsorption efficiency of test absorbent was in order of benzene > toluene > o-xylene.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

MPEG-21 표준의 구현을 위한 Testbed 구축

손정화,손현식,조영란,권혁민,김만배 강원대학교 정보통신연구소 2003 정보통신논문지 Vol.7 No.-

1990년대 후반부터 다양한 디지털 통신망을 이용하여 멀티미디어 컨텐츠 서비스가 가능하게 되었다. 하지만, 멀티미디어 컨텐츠의 전달 및 이용을 위한 기반 구조들의 독자적 발전 및 다양한 통합적 관리 체계 시스템으로 인해, 멀티미디어 컨텐츠 표현 방식의 호환성 문제, 혼재하는 네트워크 전달 방식과 단말 방식의 호환성 문제 등의 잠재적인 문제점이 발생한다. 이런 문제의 대안으로 현재 존재하는 기술 및 기반 구조들 사이의 연동을 통한 큰 프레임워크인 MPEG-21이 진행 중이다. 본 논문에서는 현재 표준화 작업이 진행 중인 MPEG-21을 기반으로 하는 Testbed를 제안한다. Testbed는 server, client, DIA(Digital Item Adaptation)의 세 모듈로 구성된다. Server의 역할은 멀티미디어 컨텐츠를 Digital Item(DI)으로 생성하고, client가 DI를 요구할 경우 DIA 모듈을 통해서 변환된 DI를 client에게 제공한다. DIA 모듈은 server에서 동작되며 client로부터 요청된 DI를 분석하고 client로부터 전송된 환경 정보를 이용하여 client 환경에 적합하게 변환된(adapted) DI를 생성하는 것이 주 기능이다. Client는 server에 저장되어 있는 DI를 선택하고 user preference, terminal capability 등의 필요한 정보를 server로 전송한다. Testbed에서는 스포츠 경기의 동영상, 정지 영상, 경기 내용, 역사를 기록한 파일 등의 DI를 이용한다. 표현 언어는 XML이며, HTTP 기반의 Web 환경에서 구동되도록 설계된다.

한국과 일본 사무작업자에 대한 Type A 行動特性과 스트레스

孫銀一,鄭玟相 진주여자전문대학 1997 論文集 Vol.20 No.-

A Questionnaire was administered to the 1674 clerk workers who work in motor company in Korea and Japan. The questionnaire included the sociodemographic status working conditions. life habits, Type A behavior question, and stress and arousal check list. In general Koreans have marks in Type A behavior, and tend to have many marks in production department. However, Japanese tend to have many marks in purchasing department. Korean had lower scores in stress than Japanese. The important stressors were hope and goal in the future work environment in Korean, but in Japanese, were home life and job satisfaction.

상아질 접착제의 미세누출에 관한 연구

손정민,최남기,김선미,양규호,박지일 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

본 연구는 여러가지 상아질 접착제들의 법랑질 변연과 상아질 변연에서의 미세누출을 비교 평가하기 위해 이루어졌다. 발거된 건전한 영구치의 협면과 설명에 5급 와동을 형성하고 형성된 50개의 와동을 무작위로 5개의 군으로 나누어 각군에 Adper™ Scotchbond™ Multi-purpose Plus Adhesive (SM; 3M ESPE. USA), Adper™ Single bond 2 (SB; 3M ESPE, USA), Clearfil™ SE Bond (SE; Kuraray Medical Inc., Japan), Adper™ Prompt™ L-Pop™ (PL; 3M ESPE, USA), G-Bond™ (GB; GC Co., Japan)를 적용하고 Filtec™ Z350 A3 (3M ESPE. USA)로 충전하였다. 시편을 37℃ 증류수에 24시간 보관한 후 5℃와 55℃에서 10초씩 1000회 열순환하고 2% methylene blue 용액을 24시간 넣어 염색한 뒤, 주수 하에 치아를 협설로 절단하여 색소의 침투 정도를 화상분석현미경을 이용하여 침투깊이를 측정한 뒤 각 군간의 미세누출 정도를 비교하여 침투깊이에 따라 0~3점으로 분류하여 다음과 같은 결과를 얻었다. 1. 범랑질 변연에서의 미세누출은 PL(0.85), SB(0.55), GB(0.50), SM(0.35), SE(0.25) 순이었으며 PL은 SM 및 SE와 통계학적으로 유의한 차이를 나타냈다(p<0.05). 2. 상아질 변연에서의 미세누출은 GB(2.10), SE(1.45), PL(1.40), SB(1.05), SM(0.70) 순이었으며 GB는 SB 및 SM과 통계학적으로 유의한 차이를 나타냈다(p<0.05). 3. 각 실험재료 군내에서 법랑질 변연에 비해 상아질 변연에서 미세누출이 더 크게 나타났으며, SE, PL, GB에서 통계학적으로 유의한 차이를 나타냈다(p<0.05). The objective of this study was to compare the microleakage of five adhesive systems in the enamel and dentin of permanent teeth. Class V cavity preparations with occlusal margins in enamel and gingival margins in dentin were prepared on both buccal and lingual surfaces of 25 extracted human molar teeth. The tested adhesives were: Adper Scotchbond Multi-purpose Plus Adhesive (SM), Adper Single bond 2 (SB), Clearfil SE Bond (SE), Adper Prompt L-Pop (pL) and G-Bond (GB). The results were as follows: 1. At the enamel margins, PL showed the highest leakage value(0.85), and others showed values of SB(0.55), GB(O.50), SM(O.35) and SE(0.25) in decreasing order. There were statistically significant differences in PL vs. SM and PL vs. SE(p<O.05). 2. At the dentin margins, GB showed the highest leakage value(2.10), and others showed values of SE(1.45), PL(1.40), SB(1.05), SM(0.70) in decreasing order. There were statistically significant differences in GB vs. SB and GB vs. SM(p<O.05). 3. Dentin margins showed high dye penetration rate than enamel margins in all material tested groups and there were statistically significant differences for SE, PL and GB.

부분 무치증 환아의 증례보고

손정민,최남기,김선미,양규호 大韓小兒齒科學會 2007 大韓小兒齒科學會誌 Vol.34 No.4

부분 무치증은 제 3대구치를 제외한 6개 이상의 선천적 치아 결손으로 정의된다. 제3대구치를 제외한 선천적 치아 결손의 유병율은 1.6∼9.6%이며 부분 무치증의 유병율은 O.08∼1.1%이다. 가장 많이 이환되는 치아는 하악 제 2소구치이며 그 다음은 상악 측절치,상악 제2소구치 순으로 호발하며 영구치에 비해 유치에서는 드물고 여성에게 호발하는 경향이 있다. 부분 무치증은 외배엽 이형성증과 같은 특정 증후군과 연관되거나 치판의 생리적인 장애나 파열,공간적 제한,치성 상피의 기능적인 비정상,기저 간엽세포의 유도 실패나 유전적 영향 등의 원인에서 유래된다고 알려져 있다. 부분 무치증은 안모의 심미적 문제와 함께 치아의 교합 이상 등 기능적인 장애가 야기될 수 있으므로 임상 검사와 방사선 검사를 통한 조기 진단이 필요하며,이에 따른 적절한 치료계획이 요구된다. 이에 본 증례에서는 특별한 전신질환이 없는 부분 무치증 환아의 구강내 소견 및치료경과에 대해 보고하고자 한다. Oligodontia is defined as the congenital absence of six or more teeth in dentition, excluding the third molars. The prevalence of congenital missing teeth is about 1.6 to 9.6% of population and the prevalence of oligidontia is about 0.08 to 1.1%. The mandibular second premolar is the most frequently absent after the third molar, followed by the maxillary lateral incisor and upper second premolar. Females seem to be affected slightly more than males. Oligodontia may occur either in isolation, or as a part of a syndrome such as ectodermal dysplasia. Different causes are possible for oligodontia: physical obstruction or distruction of the dental lamina, space limitation, functional abnormalities of the dental epithelium, failure of induction of the underlying mesenchyme, chemotherapy, radiotherapy or genetic factor. Because oligodontia would result in esthetic and functional problems, such as facial asymmetry or occlusal disharmony, early diagnosis from clinical and radiographic examination was necessary. And appropriate treatment plan should be followed. This case report was about oral conditions and treatment of the oligodontia patients who have no specific systemic disease.

shRNA-발현 아데노바이러스를 이용한 백서 심실 근세포 제2아형 이노시톨 1,4,5-삼인산 수용체의 넉 다운

손민정,이선우,우선희 충남대학교 약학대학 의약품개발연구소 2008 藥學論文集 Vol.23 No.-

Inositol 1,4,5-trisphosphate(IP3) is a second messenger that induces the release of Ca2+ from the endoplasmic/sarcoplasmic reticulum. The IP3 receptor(IP3R)-mediated signaling pathway appears to be ubiquitous and is involved in many cellular processes including cell division, fertilization and neuronal function. Although cardiac myocytes have the IP3R, its pathophysiological role has not been clearly understood because of limited selectivity of currently available pharmacological blockers. Here we show expression of type 2 IP3R(IP3R2) at the peri-nuclear region in adult rat ventricular myocytes using western blotting and immunostaining, and successful construction of IP3R2 shRNA-expressing adenovirus to knock down the protein. The expression and localization of the IP3R2 were eliminated by 6 h-infection of the shRNA-expressing adenovirus (150 multiplicity of infection) within 2 days in primary cultured adult rat ventricular myocytes. This study provides a reliable tool for probing the role of IP3R2 in intact cardiac myocytes.

향나무와 그 變種들의 類綠關係에 關한 核學的 硏究

鄭宇珪,孫玲杰,成敏雄,金鼎錫 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

In this study the relationship among Juniperus chinensis and its seven varieties-J. chinensis var. procumbens, J. chinensis var. horizontalis, J. chinensis var sargentii, J. chinensis var, kaizuka, J. chinensis var. aureo-variegata, J. chinensis var. globosa, J. chinensis var. aureo-globosa-and J. rigida was studied by using the karyological methods. The results obtained from this study were as follows : In the karyotype analysis, J. chinensis, J. chinensis var. horizontalis, J. chinensis var. sargentii, J. chinensis var. globosa, J. chinensis var. aureo-globosa, and J. rigida were diploid with 2n=22, although J. chinensis var. procumbens, J. chinensis var. kaizuka and J. chinensis var. aureo-variegata were tetraploid with 2n=44. According to descending order of the total length of the chromosomes and the length of short and long arms, from the arrangement order of chromosomes and the position of centromeres, J. chinensis var. procumbens and J. chinensis var. horizontalis were categorized into the first group J. chinensis var. kaizuka and J. chinensis var. aureo-variegata were into the second group, and J. chinensis var, globosa and J. chinensis var. aureo-globosa were into the third group, respectively. Aneuploid(2n=26) in the C-band staining of J. chinensis var. aureo-variegata was identified, although it normally showed 2n=44 as karyotype. Because light was emitted from all chromatids of the samples by fluorescent light staining with DAPI, A-T rich regions would be located all over the chromatids.

Surgical Outcomes of Adrenocortical Carcinoma; 20 Years of Experience in a Single Institution

Min Jhi Kim,Eun Jeong Ban,Soo Jung Jung,Hai Young Son1,Cho Rok Lee,Sang-Wook Kang,Jong Ju Jeong,Kee-Hyun Nam,Woong Youn Chung,Cheong Soo Park 대한갑상선-내분비외과학회 2014 The Koreran journal of Endocrine Surgery Vol.14 No.4

Purpose: Adrenocortical carcinoma (ACC) is a rare malignant tumor. Early detection is difficult and prognosis is poor. We report on 20 years of ACC surgical experience at our institution. Methods: This study included 32 ACC patients who underwent surgical resection at the Department of Surgery of the Yonsei University Health System in South Korea between January 1990 and February 2012. We reviewed these 32 patients and retrospectively analyzed long-term clinical outcomes and prognosis after radical surgery for ACC. Results: The median age of the 32 patients at diagnosis was 42.25 years (range 3∼81 years). There were 16 (50%) female and 16 (50%) male patients. Mean tumor size was 12.36 cm (range 1.8∼20 cm). Twenty-five patients (78.12%) had nonfunctioning tumors while the other seven patients (21.87%) had functioning tumors. Seventeen patients (53.12%) were classified as stage II, two (6.25%) as stage III, and 13 (40.62%) as stage IV. Fourteen patients underwent radical surgical resection, while 14 patients received adjuvant chemotherapy, two received adjuvant radiotherapy, and two received adjuvant chemoradiation. Four patients were lost to follow-up. Among the remaining 28 patients, 15 patients survived. The 5- and 10-year overall survival was 60.6% and 37.8%, respectively (median survival=85±24.3 months). Seventeen patients (53%) experienced disease recurrence. Five- and 10-year recurrence-free survival was 41.5% and 29.7%, respectively (median survival=18±5.5 months). Conclusion: Early stage at diagnosis and surgical resection were the most important prognostic factors associated with prolonged survival. The role of additional therapy remains controversial and new agents should continually be evaluated for efficacy.