한국 국립공원 산림생태계의 수목 탄소저장량 평가

이상진,박홍철,박관수,김현숙,이창민,김진원,심규원,최승운,Lee, Sang-Jin,Park, Hong-Chul,Park, Gwan-Soo,Kim, Hyoun-Sook,Lee, Chang-Min,Kim, Jin-Won,Sim, Gyu-Won,Choi, Seung-Woon 한국환경복원기술학회 2022 한국환경복원기술학회지 Vol.25 No.3

The purpose of this study is to quantitatively evaluate the amount of carbon storage for trees in forest ecosystem to support the foundation for carbon neutrality implementation in Korea National Park. It targeted 22 national parks designated and managed as national parks in Korea, and conducted research on forest trees in the terrestrial ecosystem among various natural and ecological carbon sink. The survey and analysis method followed the IPCC guidelines and the National Greenhouse Gas Inventory in Korea. The amount of tree carbon storage in the forest ecosystem of Korea National Park was confirmed to be about 218,505 thousand CO₂-ton and the amount of carbon storage per unit area was 570.8 CO₂-ton per hectare. Compared to 299.7 CO₂-ton per hectare, the average carbon storage per unit area of the entire Korean forest, it was found that about twice as much carbon was stored when assuming the same area. In other words, it means that the tree carbon storage function of the national park is about twice as high as that of the average tree carbon storage function of entire Korean forest. It has great implications in Korea National Park not only provides biodiversity promotion and exploration services as a national protected area, but also performs excellent functions as a carbon sink.

Soil characteristics of the Abies koreana communities at Korean national parks: focusing on Sobaeksan, Deogyusan, Jirisan National Parks

Chang Min Lee,Sang Jin Lee,Gwan Soo Park,Hyoun Sook Kim,Hong Chul Park 충남대학교 농업과학연구소 2022 Korean Journal of Agricultural Science Vol.49 No.2

온풍 조건에서 수분 탈락 정도에 따른 피부 분류 및 개선 방안에 대한 연구

권오선 ( Oh Sun Kwon ),강현종 ( Hyun Jong Kang ),한승민 ( Seung Min Han ),윤지선 ( Ji S Eon Yoon ),조웅희 ( Woong Hee Cho ),오주영 ( Joo Young Oh ),임준만 ( Jun Man Lim ),송영숙 ( Young Sook Song ),박선규 ( Sun Gyoo Park ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.2

피부를 탄력있고 부드럽게 하는 역할은 각질층에 존재하는 수분량에 의해 좌우된다. 피부 수분량은 냉온풍, 건조환경 등 다양한 환경 변화에 의해 영향을 받음이 알려져 있으나, 개인 피부 차이에 따른 피부수분량 변화와 회복 정도에 대해서는 많은 연구가 이루어 지지 않은 실정이다. 본 연구에서는 온풍 조건하에서 피시험자들의 피부 수분 탈락 및 회복 정도를 비교 평가하여 새로운 피부 타입을 제시하고, 온풍 조건에서 저하되는 피부 수분량을 개선 시켜주는 효능 물질을 개발하고자 하였다. 온풍 환경 조성을 위해, 건강한 피험자(남: 10 명, 여: 39 명, 25 세 - 63 세)의 전완부에 온풍(30 cm, 40 ℃, 6 m/s)을 30 min 간 피부에 노출시켜, 피부 수분량의 변화를 평가하였다. 26명(남: 4 명, 여: 22 명, 평균 연령: 42.7 ± 9.4)이 온풍 노출전에 비하여 온풍 노출 후 수분량이 유의하게 감소하며, 노출 후 30 min이 지나도 회복이 되지 않았다. 온풍노출 후 수분량이 떨어지는 피험자(여: 10 명)를 대상으로 보수력이 높은 크림을 3 주간 전완부에 적용한 이후 동일 온풍 조건하에서 피부 수분량을 측정한 결과, 노출 30 min 후 피부 수분량이 온풍 노출 전 수준으로 회복됨을 확인하였다. 본 연구를 통하여 피부는 건조 조건에서 쉽게 수분을 잃어버리는 피부(탈수형 피부)가 존재하는 것을 확인하였다. 이는 앞으로 화장품 개발을 보습 기능뿐만 아니라, 이러한 환경변화에 따른 피부수분이 쉽게 빠져나가는 피부(탈수형 피부)의 특성에 맞는 제품의 효능 개발이 필요함을 보여준다. Elasticity and softness of the skin depend on the level of moisture present in the stratum corneum, which is known to be affected by various environmental changes, such as cold and hot winds and dry environments. However, not many studies have been conducted on changes in skin moisture and the degree of recovery due to individual skin differences. In the present study, we aimed to investigate the effect of warm air heating on skin hydration levels and develop moisturizing formulas to improve lowered skin hydration levels. In order to deliver warm air heating condition, heating dryer (40 ℃, 6 m/s, 30 cm apart from forearm) was applied into inner forearm of healthy subjects (male: 10, female: 39, age: 25 - 63) Among 49 subjects, 26 subjects showed significantly lowered skin hydration levels until 30 min after warm air heating exposure (lowered group). In addition, moisturizing cream with high water holding capacity was applied to forearm of 10 subjects in lowered group for 3 weeks and skin hydration levels after warm air heating were significantly improved at the levels of before application of warm air heating. From this study, we found out that there is a skin type that skin hydration levels are significantly decreased under warm air heating condition (dehydrated skin) and this dehydrated skin can be improved by moisturizing formulas with high water holding capacity.

원광의대 부속 제 2병원 신경정신과 환자에 대한 통계적 고찰

박민철,김종주,박남진,오상우,권혁일,권영숙,박종호 圓光大學校 醫科學硏究所 1986 圓光醫科學 Vol.2 No.2

We examined 1,856 patients who visited Won Kwang Neuropsychiatric hospital since July 11 of 1984(since the hospital opened) until June 11 of 1986. We researched diagnostic and other distributions of inpatients and outpatients. The study revealed as follows : 1. Of total patients males visited this hospital more than females as 52.7%. Most of them(67.1 %) resided in Iri, Iksan and Kimje. In the point of insurance, insured patients and uninsured patients except medicaids were 73 %. Males(60%) were more insured medically than females (55.4%). While insured patients relatively resided in urban area, but uninsured patients except medicaids did in rural area. 2. Of outpatients neurotic disorder was the most as 35.1%, secondly schizophrenic disorder and thirdly affective disorder, but of inpatients schizophrenic disorder was the most as 41.4%, secondly neurotic disorder thirdly affective disorder. 3. Alcohol abuse (98.2 %), paranoid disorder, convulsive disorder and child mental disorder were mostly found in males, but neurotic disorder (59 .6 %), affective disorder and other psychotic disorder were mostly found in females. 4. In unmarried ones schizophrenic disorder ( 43 .6 %), neurotic disorder, other psychotic disorder were common, but in married ones neurotic disorder (55.1 %), schizophrenic disorder, affective disorder were common. 5. On the distribution of age schizophrenic disorder was found the most in the twenties (28.4 %), next in the thirties and in the forties, but neurotic disorder was found the most in the thirties, next in the twenties and in the forties. 6. On the distribution of age at onset schizophrenic disorder develops mostly during the twenties (45.5 %), secondly during the teens and thirdly during the thirties, but neurotic disorder develops mostly during the thirties and the twenties (53.1 %). 7. On the duration of hospitalization 34.4% of the patients was hospitalized during less than 15 days, 22.0% during 60-90 days and 80.4% during less than 3 months. 8. In the result of treatment 41.2% of the patients was improved and 31.7% a little improved. In total 72.9% of the patients got helped therapeutically.

아버지의 대화태도와 유아의 사회적 문제해결 능력

박민숙,이정숙 漢陽大學校 韓國生活科學硏究所 1997 韓國 生活 科學 硏究 Vol.- No.15

The purpose of the study was to investigate the correlation between father's conversational attitude, his educational level and his child's ability of the social problem settlement. It was also involved in the investigation whether father's conversational attitude was different with the child's gender and whether the child's ability of solving the social problems was affected by the gender. First, the static correlation turned out to be between father's conversational attitude and his child's ability of solving the social problems. The child had better ability whose father got high marks in conversational attitude with accepting attitude than the child of father who got low marks. Second, no meaningful difference by father's educational level was found in child's ability of solving the social problems. Third, no meaningful difference by child's gender was found in father's conversational attitude. This finding indicated that lather had a fair conversational attitude toward his children regardless their gender. Most of the subjected children's personality according to the trend of small family. In addition, the child whom teacher judged as being adaptable had better ability of coping with the social problems than the child whom teacher judged as being impulsive or daunted. Based upon the findings, it could be said that father's conversational attitude had an influence on child's solution of the social problems which constituted the social recognition. As the chief persons who brought up a child, father as well as mother had a big influence on child's perception and emotion. To educate fathers in how to think and how to speak would result in not only the advancement of child's perception and positive relationship wish others but also the improvement of gather's conversational attitude.

DNA 및 유전자 발현의 전자현미경 관찰

박민희,이숙영,박열,김성준 조선대학교 기초과학연구소 1989 自然科學硏究 Vol.12 No.1

본 연구는 전자현미경에 의해서 플라스미드 DNA의 모양과 크기 및 염색체 DNA의 유전자 발현 과정을 관찰하였다. 전자현미경 관찰을 위하여 본 연구에서는 Miller chromatin-spreading법과 micapress adsorption법을 이용하여, pBN_18, pBN_18A, pBN_18B 그리고 pBR_322 플라스미드 DNA들을 관찰하였다. 그 결과 이들의 크기가 각각 1.87±0.28(5.6kb)μm, 1.53±0.12(4.6Kb)μm, 1.53±0.12(4.6Kb)μm, 그리고 1.50±0.03(4.50Kb)μm이었음을 micapress adserption법에 의하여 측정하였다. 그리고 Miller chromatin-spreading법을 이용한 chromosomal DNA의 유전자 발현과정의 관찰로 transcription을 관찰함으로써 전사과정이 활발한 유전자 부위와 전사과정이 활발하지 않은 유전자 부위를 관찰할 수 있었다. 따라서 세포내에서 방출된 플라스미드 DNA를 선별적으로 관찰헐 수 있는 micapress adsorption법을 이용하여 분자량이 작은 플라스미드의 관찰을 신속하고 유용하게 할 수 있었으며, 플라스미드 DNA들은 2가 양이온이 없어도 mica에 잘 흡착되어졌다. 또한 Miller chromatin-spreading법은 E. coli에서 유전자 발현에 고나한 초미세구조적 관찰로 개개의 유전자 수준에서 조절기작을 연구할 수 있게 하며 유전자들의 replication과 transcription 수준에서의 물리적 상호작용을 관찰하는데 적용될 수 있을 것으로 사료된다. This study was visualized the transcriptional activity, the size and the model of plasmid DNA by electron microscopy. Micapress adsorption method that allows selective visualization of the plasmid DNA released in situ from the bacterial cell is rapid and useful for visualization of plasmids. The released plasmid DNA was adsorbed preferentially on mica in a divalent cation-free solution, and the Muller chromatin-spreading method may allow the physical interaction and transcription to be visualized. Therefore micapress adsorption method and Miller chromatin-spreading method used to visualize the plasmid DNA, chromosomal DNA and gene expression. As a result, their estimated sizes of plasmid pBN18, pBN18A, pBN18B and pBR322 were 1.87±0.28(5.6Kb)㎛, 1.53±0.12(4.6Kb)㎛, 1.53±0.12(4.6Kb)㎛, and 1.50±0.03(4.50Kb)㎛, respectively, and transcriptional activity was observed by the visualization of gene expression of chromosomal DNA.

갑상선 침세포진 시료의 주사전자현미경적 관찰

박성진,이숙희,조창호,채종민 慶北大學校 醫科大學 1995 慶北醫大誌 Vol.36 No.3

목적 : 갑상선 결절에 대한 수술전 진단을 위하여 침세포진 검사는 매우 유효하며 빈번히 시행되고 있다. 그러나 집단으로 도말된 세포들에서 때때로 종양세포의 감별진단이 어려운 경우가 있다. 주사전자현미경에 의한 세포들의 배열이나 세포 표면에 대한 관찰은 매우 드물다. 본 연구는 이러한 침세포진 검사에 있어서 주사전자현미경적 관찰을 감별 진단에 이용하고자 한다. 대상 및 방법 : 1994년 5월부터 1995년 4월까지 경북대학교병원에서 갑상선 침세포진 검사를 하고 갑상선 절제술을 시행하여 확진된 56례의 시료를 주사전자현미경으로 관찰하였다. 결과 : 결절성 비대증을 포함한 양성 병변에서는 상피세포 표면에 미세 응모가 잘 발달되어 있었으나 악성 종양의 상피세포 표면에는 미세 융모의 감소 및 완전 소실이 관찰되었다. 그러나 유두상암종에서는 미융모가 발달되어 있었으나 매우 불규칙하였다. 그리고 광학현미경상 혼돈이 되는 소포성 및 유두상 구조물의 감별이 용이하였다. 결론 : 이상의 성적으로 보아 갑상선 결절성 병변의 침세포진 검사에 있어서 감별 진단이 어려운 경우 이를 주사전자현미경으로 관찰하는 것이 감별진단에 도움이 될 것이라고 생각된다. Fine needle aspiration cytology is widely accepted as a primary method for the diagnosis of thyroid lesions. To ascertain the effective value of scanning electron microscopic features of fine needle aspiration cytologic specimens, the scanning electron microscopic study of 56 various thyroid lesions was carried out. Well developed microvilli were observed in the follicular surface almost every epithelial cell in the nodular hyperplasia. Their number was reduced in adenomas and differentiated carcinomas. Medullary carcinomas and anaplastic carcinomas usually lack microvilli. Papillary carcinomas on the other hand have abundant microvilli, but their shape was much irregular. Follicular and papillary structures were easily identified by scanning electron microscopic observation. The results suggested that observing by scanning electron microscopy of fine needle aspiration cytologic specimens may be helpful in the differential diagnosis of thyroid lesions.

The Protein and Isozyme Patterns During in vitro Plant Regeneration of Yooja (Citrus junos Sieb.) and Trifoliate Orange (Poncirus trifoliata Rafin.)

Park,Min-Hee,Jang,Hyun-Kyu,Cha,Young-Ju,Kim,Ho-Bun,Lee,Sook-Young 한국자원식물학회 2002 Plant Resources Vol.5 No.1

In this study, plant regeneration through in vitro culture from plantlet stems of Yooja (C. junos Sieb.) and trifoliate orange (P. trifoliata Rafin.) was attempted to make mass-production system of virus-free plants having the same genotype with mother plant. In order to investigate physiological change depending on the developmental stage of plant regeneration, the changes of total protein, peroxidase and esterase activity and their isozyme patterns as well were examined in 1/2 MS medium. The results are as follows : 1. The MS medium for the optimal callus induction and shoot formation was utilized. The medium was supplemented either with 2,4-D and Kinetin or with BA and NAA. The optimal concentrations were the combination of 1.0mg/ 2,4-D +0.3mg/ Kinetin and 1.0mg BA +0.3mg NAA in callus induction and shoot formation, respectively. 2. For the plant regeneration from somatic embryos, 1/2 MS medium was used with supplements of growth regulators (free, 1.0mg/ IBA +1.0mg/ BA ,0.5mg/ IBA +0.5mg/ BA). Shooting and rooting were the best in the treatment of 0.5mg/ IBA and 0.5mg/ BA combination. 3. The total protein content has a tendency of increase with the developmental stage of embryo, but it was decreased at the plantlet. Also it was the highest at 8 and 6 weeks stage in C. junos Sieb. and P. trioliata Rafin, respectively. In the SDS-PAGE pattern of protein, C. junos Sieb. showed bands of 29.0 and 40kDa at 10 weeks. The 45,66 and 97.4 kDa bands at 10 weeks of culture were shown in P. trifoliata Rafin. 4. The highest esterase activity was shown at the 6 and 8 weeks of culture in C.junos Sieb. and P. trifoliata Rafin.., respectively. 5. Esterase isozyme patterns were shown difference according to the developmental stage. In C. junos Sieb. a new band was observed at pl 7.7 following 4 weeks culture. On the other hand, new bands in P. trifoliata Rafin. were observed at pl 7.5~6.5 following 4 and 6 weeks culture, respectively.

4종의 식품 부패 미생물에 대한 국내산 자생 식물 열매 추출물의 항균성 탐색

권민경,이해은,박주연,한영숙 동아시아식생활학회 2003 동아시아식생활학회지 Vol.13 No.5

To select new useful domestic plants with antimicrobial activities, thirty five samples of berry plant were distributed from Plant Diversity Research Center in Korea Research Institute of Bioscience and Biotechnolog. Their extracts with methanol were tested against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Listeria monocytogenes by paper disc method. The methanol extracts from Viburnum dilatatum, Viburnum erosum, Aralia continentalis, Camellia japonica, Acer trumcatam, Arasaema takesimense and Rhysocarpus intermedius were effective against S. aureus and the results were as follows: Viburnum erosum 6.5 ㎜, Viburnum dilatatum and Aralia continentalis 7.0 ㎜, Rhysocarpus intermedius 8.0 ㎜, Acer to mcatum 9.0 ㎜, Camellia japonica and Arisaema takesimense 9.5 ㎜ And only the methanol extract from Camellia japonica was effective against L. monocytogenes, 7.0 ㎜. Antimicrobial activities of E. coli and S. typhymurium were not detected. The minimum inhibitory concentration(NIC) of berry of Camellia japonica was examined as 1,250 ㎍/mL against S. aureus and as 1,250 ~ 2,500 ㎍/mL against L. monocytogenes.

Coco Chanel의 作品世界와 그 現代的 意味

구인숙,박춘순,백민숙 충남대학교 자연과학연구소 1987 忠南科學硏究誌 Vol.14 No.1

Poiret was the first innovator of the new fashion of the twentieth century. Chanel was the second innovator. It is Chanel who saw positively the great spirit of the twentieth century and showed modern emancipated women how to dress. Chanel conveyed their headiness and independence in her clothes. She thought it would be chic to dress them lie working girls and she made the Garconne look, with its flat-bosomed silhouette bounded by straight lines. This look in fashion was closely related to the cubist movement in art popular at that time. Chanel expressed the casual simplicity, comfortness, function in her clothes and Chanel style had the smart, understated(Deluxe poor look), easy-to-wear looks. Chanel wanted to dress all women and had great understanding of the value of publicity as a fashion force. With Chanel-designed clothes, the wholesale apparel business therived. Her simplified clothes lent themselves to mass production as the elaborate creations before her could not do.