Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells

Zhang Ying,Wang Peng,Jin Mei-xian,Zhou Ying-qi,Ye Liang,Zhu Xiao-juan,Li Hui-fang,Zhou Ming,Li Yang,Li Shao,Liang Kang-yan,Wang Yi,Gao Yi,Pan Ming-xin,Zhou Shu-qin,Peng Qing 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.3

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs. METHODS: hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated. RESULTS: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2–related factor 2 signaling. CONCLUSION: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.

Different genomic DNA methylation patterns between male and female adults of white-backed planthoppers Sogatella furcifera

Mei Zhang,Jia-Lin Chen,Xiao-Sui Zhou,Shi-Ke Liang,Guang-Hong Li,Fang-Hai Wang 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4

DNAmethylation plays a key role in gene regulation and phenotype variation in many organisms. The aimof thisstudy was to survey the frequency and variation of cytosine methylation at CCGG sequences in adult male andfemale planthoppers Sogatella furcifera, a major rice pest in Asia, and to determine the occurrence ofmethylationchanges associatedwith sexual dimorphismusingmethylation-sensitive amplification polymorphism. 1131DNAfragments including CCGG siteswere amplified using 36 pairs of selective primers: about 191methylated bandswere identified. Inmale planthoppers,we got a total of 581 bands, including 40 fully-methylated bands, 65 hemimethylatedbands and 476 none-methylated bands, so the fully-methylated ratio, hemi-methylated ratio andtotal methylated ratio were 6.88%, 11.19% and 18.07%, respectively. In the female planthopper, there were atotal of 550 bands, including 44 fully-methylated bands, 42 hemi-methylated bands and 464 none-methylatedbands. The fully-methylated ratio was 7.64% in female planthoppers, which was slightly higher than in themale planthoppers, however, the hemi-methylated ratio was lower (8.00%) in the female compared with themale planthopper. Altogether, 46 DNA bands displayed variable cytosine methylation patterns between maleand female samples: 20 of themoccurred only inmale samples and 26 only in female samples. Thus, the genomemethylation patterns are different between male and female adults. The results suggest that DNA methylationmight be related to sexual differentiation and development in S. furcifera.

Preliminary study of genome-wide association identified novel susceptibility genes for thyroid-related hormones in Chinese population

Huang Liang,Bai Fenghua,Zhang Yutian,Zhang Shanshan,Jin Tianbo,Wei Xingwei,Zhou Xiaoli,Lin Mei,Xie Yufei,He Chanyi,Lin Qi,Xie Tian,Ding Yipeng 한국유전학회 2022 Genes & Genomics Vol.44 No.8

Background: Thyroid hormones are critical regulators of metabolism, development and growth in mammals. However, the genetic association of thyroid-related hormones in the Chinese Han population is not fully understood. Objective: We aimed to identify the genetic loci associated with circulating thyroid-related hormones concentrations in the healthy Chinese Han population. Methods: Genotyping was performed in 124 individuals using Applied Biosystems™ Axiom™ PMDA, and 796,288 single nucleotide polymorphisms (SNPs) were available for the GWAS analysis. For replication, eleven SNPs were selected as candidate loci for genotyping by Agena MassARRAY platform in additional samples (313 subjects). The values of p < 5 × 10- 6 suggest a suggestively significant genome-wide association with circulating thyroid-related hormones concentrations. Results: We identified that rs11178277 (PTPRB, p = 4.88 × 10- 07) and rs7320337 (LMO7DN-KCTD12, p = 1.22 × 10- 06) were associated with serum FT3 level. Three SNPs (rs4850041 in LOC105373394-LINC01249: p = 3.55 × 10- 06, rs6867291 in LINC02208: p = 2.40 × 10- 06 and rs79508321 in WWOX: p = 3.35 × 10- 06) were related to circulating T3 level. Rs12474167 (LOC105373394-LINC01249, p = 1.65 × 10- 06) and rs1864553 (IWS1, p = 2.00 × 10- 06) were associated with circulating T4 concentration. The association with TGA concentration was for rs17163542 in DISP1 (p = 3.46 × 10- 06) and rs12601151 in NOG-C17orf67 (p = 2.72 × 10- 07). Two genome-level significant SNPs (rs2114707 in LINC01314, p = 1.69 × 10- 06 and rs12601151, p = 1.41 × 10- 07) associated with serum TMA concentration were identified. Moreover, rs6083269 (CST1-CST2, p = 3.36 × 10- 06) was a significant locus for circulating TSH level. In replication, rs12601151 in NOG-C17orf67 was still associated with serum TGA level (p = 0.012). Conclusions: The GWAS reported 11 new suggestively significant loci associated with circulating thyroid-related hormones levels among the Chinese Han population. These findings represented suggestively biological candidates for circulating thyroid-related hormones levels and provided new insights into the mechanisms of regulating serum TGA concentration.

Biochemical Characterization of a GDSL-Motif Esterase from Bacillus sp. K91 with a New Putative Catalytic Mechanism

( Jun Mei Ding ),( Ting Ting Yu ),( Lian Ming Liang ),( Zhen Rong Xie ),( Yun Juan Yang ),( Jun Pei Zhou ),( Bo Xu ),( Jun Jun Li ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11

The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around 50°C at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an α/β-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.

Ex vivo Expansion of Bone Marrow Mesenchymal Stem Cells Using Microcarrier Beads in a Stirred Bioreactor

Lang Zhou,Jiantao Kong,Ying-ping Zhuang,Ju Chu,Si-Liang Zhang,Mei-Jin Guo 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1

Bone marrow mesenchymal stem cells (bmMSCs)have recently gained attention as a useful resource in the fields of regenerative medicine and tissue engineering. However, the number of bmMSCs obtained from available donors is very low. Here we developed a culture strategy for in vitro expansion of bmMSCs in a 1.5 L stirred bioreactor with microcarrier beads. First, the microcarriers (Cytodex 3) were equilibrated in culture medium containing 3% fetal bovine serum (FBS) for at least 30 min prior to cell addition. After inoculation, the FBS concentration of the medium was maintained at 3% (v/v) in the first 24 h and thereafter maintained at 1% (v/v) and a developed feeding regimen was applied over 5 days. The maximum cell density of 2.6 × 106 cells/mL was achieved at day 5,corresponding to a 10.4 ± 0.8 fold increases in total cell number. Among the harvested cells, 98.95% expressed CD29and 84.48% expressed CD90, suggesting that the majority of expanded bmMSCs still retained their differentiation potential. Therefore, the developed microcarrier-based stirred bioreactor culture system is an effective method to generate significant numbers of bmMSCs for potential applications and research studies.

Effects of Ti nonstoichiometry on microstructure, dielectric, ferroelectric and piezoelectric properties of (Na0.54Bi0.5)0.94Ba0.06Ti1+xO3 lead-free ceramics

Xu-mei Zhao,Xiao-Ming Chen,Jian Wang,Xiao-xia Liang,Jian-Ping Zhou,Peng Liu 한양대학교 세라믹연구소 2015 Journal of Ceramic Processing Research Vol.16 No.1

The lead-free ceramics (Na0.54Bi0.5)0.94Ba0.06Ti1+xO3 with x = −0.02, 0, 0.02, and 0.04 were prepared by a solid-state reaction method. Effects of Ti4+ nonstoichiometry on crystallite structure, microstructure, dielectric, ferroelectric and piezoelectric properties were studied. The ceramics with x = −0.02 and 0 exhibit typical diffraction peaks of perovskite structure. A secondary phase appears in the ceramics with x = 0.02 and 0.04. The rhombohedral-tetragonal morphotropic phase boundary exists in all samples, and the relative amount of the tetragonal phase changes with the increase in the Ti4+ amount from deficiency to excess. Grain sizes and shape of the ceramics are unaffected by changes in Ti4+ nonstoichiometry. But, dielectric, ferroelectric and piezoelectric properties of the ceramics are close related to the Ti4+ amounts. Compared with the ceramic with x = 0, the ceramics with the Ti4+ nonstoichiometry have higher depolarization temperature, lower maximum dielectric constant, decreased piezoelectric constant and ferroelectric properties.

Raindrop Category Numerical Modeling on Microphysical Process of Precipitation Formation of Stratiform Cloud in Northern China

Xue-Liang Guo(꿔쒜량),Yong-Seung Chung(정용승),Mei-Yuan Huang(황메이완),Ling Zhou(쪼링) 한국기상학회 1998 Asia-Pacific Journal of Atmospheric Sciences Vol.34 No.4

Dye-Sensitized-Assisted, Enhanced Photocatalytic Activity of TiO2/Bi4V2O11

Mengjun Liang,Zhiyuan Yang,Ying Mei,Haoran Zhou,Shuijin Yang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.3

In this study, the TiO2/Bi4V2O11 nanocomposite photocatalysts were prepared by loading different amount of TiO2 nanoparticles onto the surface of Bi4V2O11 nanospheres via a facile hydrothermal method. Afterwards, the as-synthesized samples were characterized by high-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), N2 adsorption–desorption isotherms, X-ray photoelectron spectroscopy (XPS), UV-Vis diffuse reflectance spectroscopy (DRS) and photocurrent techniques. The optimal TiO2/Bi4V2O11 composite with 20 wt.% TiO2 loading (TB2) exhibited the best photocatalytic activity, which could degrade almost RhB completely within 30 min under visible light irradiation. The enhanced photocatalytic activity of TiO2/Bi4V2O11 composites for RhB degradation could be mainly ascribed to the efficient charge separation over dye-induced sensitized and the increased specific surface area. Also, the photocatalytic activities of TiO2/Bi4V2O11 for CIP degradation were tested. After five consecutive recycling experiments, the photocatalytic degradation activity of TB2 could still reach 99% which indicated that the catalysts had superior stability. Based on the experimental and bandgap calculations, a possible photocatalytic mechanism of TiO2/Bi4V2O11 for RhB degradation was proposed.

Raindrop Category Numerical Modeling on Microphysical Process of Precipitation Formation of Stratiform Cloud in Northern China

Guo,Xue-Liang,Huang,Mei-Yuan,Chung,Yong-Seung,Zhou,Ling 한국교원대학교 환경과학연구소 2000 환경연구논문집 Vol.- No.5

중국 북부지방의 관측자료와 강우 category model을 이용하여 강수 발생의 미세과정과 전형적인 층운 3가지 종류가 형성될 때의 빙정 역할이 분석되었다. 종류 Ⅰ는 강한 전선면의 광범위한 상승에 따른 층운의 형성으로서 강우의 발생은 주로 수증기의 침전과 상층 구름 속 빙정위의 riming과정에 좌우된다. 이때 빙정은 높은 구름에 매우 큰 농도로 존재하여 강수의 형성에 중요한 역할을 하며 이 구름 종류의 seeder-feeder 과정으로 고려된다. 종류 Ⅱ는 저기압 규모의 수렴에 의해 형성되는 층운으로서 riming에 의해 성장되는 빙정과정에 따라 발생되는 강수이다. 종류 Ⅲ은 약한 전선면상에 발생되는 광범위한 상승 기류에 동반되어 낮은 구름층에서 형성된다. 구름 방울들의 충돌과 구름 방울 및 빗방울 사이의 충돌은 이런 강우 형성의 중요 과정임이 밝혀졌다. Based on the observations in northern China, the microphysical process of precipitation formation and the role of ice crystal playing in this process for three kinds of typical stratiform clouds are analyzed using rain category model. Results show that for type one, namely stratiform cloud system formed by widespread lifting of a strong frontal surface, the original formation of rainfall depends mainly on vapor deposition and riming process on ice particles from aloft. Ice crystal has a high number concentration in high level of cloud due to the lower temperature and plays a very important role in the formation of precipitation and shows clearly the "seeder-feeder" process in this kind of cloud; For type two, namely stratiform cloud system produced by cyclonic scale convergence which provides liquid water, the primary precipitation is due to the process of ice crystal growing by riming; Type three is the shallow layer cloud system associated with widespread lifting of a weak frontal surface system.

Breeding of L(+)-Lactic Acid Producing Strain by Low-Energy Ion Implantation

( Ge Chun Mei ),( Shao Bin Gu ),( Xiu Hong Zhou ),( Jian Ming Yao ),( Ren Rui Pan ),( Zeng Liang Yu ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.2