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HONG, SANG-WON,JUNG, KYUNG HEE,CHOI, MYUNG-JOO,KIM, DA YOUNG,LEE, HEE-SEUNG,ZHENG, HONG-MEI,LI, GUANG YONG,EL-DEEB, IBRAHIM M.,PARK, BYUNG SUN,LEE, SO HA,HONG, SOON-SUN Spandidos Publications 2012 International journal of oncology Vol.41 No.5
<P>The anticancer effect of a new pyrazole derivative, KI-10F (2-(4-(2-(4-(dimethylamino) phenyl)pyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol?1-yl) acetonitrile)?3.5HCl) was evaluated in human colon cancer cells. KI-10F strongly suppressed the growth of human colon cancer cells and induced apoptosis by increasing the proportion of sub-G1 presenting apoptotic cells as well as causing cell cycle arrest at the G2/M phase. Apoptosis by KI-10F was confirmed by observation of an increase in the expression of cleaved caspase-3, caspase-8, caspase-9 and Bax, and the decrease of Bcl-2. Decreased expression of HIF-1α and VEGF, and the inhibition of HUVEC tube formation and migration showed that KI-10F effectively inhibited the angiogenesis process. Furthermore, in?vivo study in a mouse xenograft model showed that KI-10F produced a stronger antitumor activity than 5-FU, a conventional anticancer drug prescribed for the treatment of colon cancer. The effects of KI-10F on tumor proliferation (PCNA), angiogenesis (CD34) and apoptosis (cleaved caspase-3) were evaluated by immunohistochemistry using isolated tumor tissue samples. Taken together, our results demonstrated that KI-10F induces apoptosis and inhibits cell growth and angiogenesis both in?vitro and in?vivo. We suggest that KI-10F is an effective chemotherapeutic candidate for use against colon cancer.</P>
Structural health monitoring of innovative civil engineering structures in Mainland China
Li, Hong-Nan,Li, Dong-Sheng,Ren, Liang,Yi, Ting-Hua,Jia, Zi-Guang,LI, Kun-Peng Techno-Press 2016 Structural monitoring and maintenance Vol.3 No.1
This paper describes the backgrounds, motivations and recent history of structural health monitoring (SHM) developments to various types of engineering structures. Extensive applications of SHM technologies in bridges, high-rise buildings, sport avenues, offshore platforms, underground structures, dams, etc. in mainland China are summarily categorized and listed in tables. Sensors used in implementations, their deployment, damage identification strategies if applicable, preliminary monitoring achievements and experience are presented in the lists. Finally, existing problems and promising research efforts in civil SHM are discussed, highlighting challenges and future trends.
Guang-tao Li,Deng-pan Qiao,Hong-sheng Sun,Li-hua He 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.12
In order to improve the production efficiency of mine and ensure production safety and aiming at the unsatisfying blasting effects and uneven rock fragments existing in the exploitation blasting and construction process in Manjiazhai stope of Hualian Zinc and Indium Stock company, this paper analyzes and optimizes loosening blasting parameters of Open-pit deep-hole benches based on the blasting mechanism and engineering practical experience of wide hole distance and small resistance lines. Utilizing the numerical simulation of finite element software with ANSYS/LS-DYNA various schemes, analysis validations on the blasting stress field under different parameters are conducted. The simulation results show that: When the holes and rows spacing are 15×9, 16×9, 17×8, the effective stress of discrete cells between blast holes is bigger than the dynamic tensile strength that rocks can be broken. The site experimental investigation is conducted. The blasting effect assessment shows that the control rates of the big size rocks when the holes and rows spacing are 16×9 is 1.904% lower than those when those are 15×9, and 1.079% lower than those are 17×8. And the shovel mucking efficiency and composite economic results are better than that of the other two groups of parameters and confirmed the best blasting parameter design.
Li, Li-Ping,Chen, Di-Yang,Li, Shu-Cai,Shi, Shao-Shuai,Zhang, Ming-Guang,Liu, Hong-Liang Techno-Press 2017 Geomechanics & engineering Vol.13 No.6
The geological conditions surrounding the Jijiapo Tunnel of the Three Gorges Fanba Highway project in Hubei Province are very complex. In this paper, a 3-D physical model was carried out to study the evolution process of filling-type fracture water inrush and mud gush based on the conditions of the section located between 16.040 km and 16.042 km of the Jijiapo Tunnel. The 3-D physical model was conducted to clarify the effect of the self-weight of the groundwater level and tunnel excavation during water inrush and mud gush. The results of the displacement, stress and seepage pressure of fracture and surrounding rock in the physical model were analyzed. In the physical model the results of the model test show that the rock displacement suddenly jumped after sustainable growth, rock stress and rock seepage suddenly decreased after continuous growth before water inrushing. Once water inrush occured, internal displacement of filler increased successively from bottom up, stress and seepage pressure of filler droped successively from bottom up, which presented as water inrush and mud gush of filling-type fracture was a evolving process from bottom up. The numerical study was compared with the model test to demonstrate the effectiveness and accuracy of the results of the model test.
( Xi Hong Zhao ),( Yan Mei Li ),( Myoung Su Park ),( Jun Wang ),( You Hong Zhang ),( Xiao Wei He ),( Fereidoun ),( Forghani ),( Li Wang ),( Guang Chao Yu ),( Deog Hwan Oh ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.2
In this study, a loop-mediated isothermal amplification (LAMP) method to rapidly detect Staphylococcus aureus strains was developed and evaluated by extensively applying a large number of S. aureus isolates from clinical and food samples. Six primers were specially designed for recognizing eight distinct sequences on the species-specific femA gene of S. aureus. The detection limits were 100 fg DNA/tube and 104 CFU/ml. The LAMP assay was applied to 432 S. aureus strains isolated from 118 clinical and 314 food samples. Total detection rates for the LAMP and polymerase chain reaction assays were 98.4% (306/311) and 89.4% (278/311), respectively.
Microwave-assisted Approach for the Rapid Enzymatic Digestion of Rapeseed Meal
Ju-Fang Li,Fang Wei,Lu-Lu Guo,Gang-You Yuan,Feng-Hong Huang,Mu-Lan Jiang,Yuan-Di Zhao,Xu-Yan Dong,Guang-Ming Li,Hong Chen 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.2
This study demonstrates the use of a new microwave-assisted approach for accelerating the enzymatic digestion of rapeseed meal. The effects of different microwave parameters, such as the time, temperature, and power level, on the degree of hydrolysis (DH) were investigated by using response surface methodology (RSM). The maximum predicted DH value (10.2%) was in good agreement with the value obtained experimentally using an alkaline protease, which was 12.57% under optimal conditions. In only 7 min, the microwave-assisted method achieved a DH value similar to that obtained by the conventional enzymatic digestion method (4 hr). Therefore,this new technique for rapid enzymatic digestion will improve the application of rapeseed meal in the preparation of protein hydrolysates for use in food and feed.
Cong, Xin,Zhang, Xue-Ming,Zhang, Yan,Wei, Tai,He, Qi-Hua,Zhang, Li-Wei,Hua, Hong,Lee, Sang-Woo,Park, Kyungpyo,Yu, Guang-Yan,Wu, Li-Ling Elsevier 2018 Biochimica et biophysica acta. Molecular basis of Vol.1864 No.10
<P><B>Abstract</B></P> <P>Sjögren's syndrome (SS) is an inflammatory autoimmune disease that causes hyposecretion in salivary glands. Endothelial tight junctions (TJs) play crucial roles in salivation and barrier function of blood vessels. However, whether the alteration of endothelial TJs were involved in pathogenesis of SS was still unknown. Here, the ultrastructure and function of endothelial TJs in submandibular glands (SMGs) were detected by transmission electron microscopy and in vivo paracellular permeability assay in different aged NOD mouse model for SS. CFSE-labeled lymphocytes were injected into tail vein to trace the infiltration, while claudin-5 expression and distribution were detected by immunofluorescence, qRT-PCR, and western blot. Results showed that the stimulated salivary flow rate was gradually decreased and lymphocytic infiltration was found as age increased in 12- and 21-week-old NOD mice, but not 7-week-old NOD mice. Blood vessels were dilated, while endothelial TJ width and paracellular tracer transport were increased in 12-week-old NOD mice. Moreover, the injected CFSE-labeled lymphocytes were observed in SMGs of 12-week-old NOD mice. Claudin-5 level was increased and relocalized from the apical portion of neighboring endothelial cells to lateral membranes and cytoplasm in 12-week-old NOD mice. Additionally, the alteration of claudin-5 expression and distribution was further confirmed in labial salivary glands and bilateral parotid glands from SS patients. In cultured human microvessel endothelial cell line (HMEC-1), IFN-γ stimulation significantly increased claudin-5 expression. Taken together, we identified that the endothelial TJ barrier was disrupted and contributed to the development of salivary hyposecretion and lymphocytic infiltration in SS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Endothelial tight junction barrier is disrupted in hyposecretory submandibular glands from Sjögren's syndrome mouse model </LI> <LI> The disrupted salivary endothelial barrier is linked with lymphocytic infiltration in Sjögren's syndrome mouse model </LI> <LI> The redistribution of claudin-5 is responsible for disrupted endothelial barrier in salivary glands from Sjögren's syndrome </LI> </UL> </P>
A Generalized Formula for Elastic Shear Buckling of Trapezoidal Corrugated Web Girder
Hong-Guang Luo,Li-Ying Peng,Chan-Tao Zhang,Cheng-Xiu Cai 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.10
Trapezoidal corrugated web girder is widely used. The elastic shear buckling stress is an important parameter in the design and calculation of trapezoidal corrugated web girder. Accordingly, much research has been devoted on the analysis of typical elastic global shear buckling, elastic local buckling and elastic interactive shear buckling, and the corresponding formulae existing in a variety of literature. The results of finite element analysis suggest that there is a certain deviation in the calculated values based on classical elastic shear buckling formulae. The boundary conditions of corrugated web play an important role in the calculation of elastic shear buckling. And it is found that the shear buckling modes of the actual trapezoidal corrugated web may be called the atypical buckling, which is different from typical local buckling, global buckling and interactive buckling. The existence of the atypical buckling may result in some calculation deviations for the existing formulae of elastic shear buckling. Based on the classical elastic shear buckling formulae, a generalized formula for elastic shear buckling of trapezoidal corrugated web girder is obtained by numerical simulation. It intuitively reflects the relationships among various buckling modes. The formula has high calculation reference value and will promote the research of elastic shear buckling of trapezoidal corrugated web girder.