A Single-Arm Phase II Study of Nab-Paclitaxel Plus Gemcitabine and Cisplatin for Locally Advanced or Metastatic Biliary Tract Cancer

Ting Liu,Qing Li,Zhen Lin,Chunhua Liu,Wei Pu,Shasha Zeng,Jun Lai,Xuebin Cai,Lisha Zhang,Shuyang Wang,Miao Chen,Wei Cao,Hongfeng Gou,Qing Zhu 대한암학회 2024 Cancer Research and Treatment Vol.56 No.2

Purpose Patients with advanced biliary tract cancer (BTC) have a poor survival. We aim to evaluate the efficacy and safety of nab-paclitaxel plus gemcitabine and cisplatin regimen in Chinese advanced BTC patients.Materials and Methods Eligible patients with locally advanced or metastatic BTC administrated intravenous 100 mg/m² nab-paclitaxel, 800 mg/m² gemcitabine, and 25 mg/m² cisplatin every 3 weeks. The primary endpoint was progression-free survival (PFS). The secondary endpoints included overall survival (OS) and adverse events, while exploratory endpoint was the association of biomarkers with efficacy.Results After the median follow-up of 25.0 months, the median PFS and OS of 34 enrolled patients were 7.1 months (95% confidence interval [CI], 5.4 to 13.7) and 16.4 months (95% CI, 10.9 to 23.6), respectively. The most common treatment-related adverse events at ≥ 3 grade were neutropenia (26.5%) and leukopenia (26.5%). Survival analyses demonstrated that carcinoembryonic antigen (CEA) levels could monitor patients’ survival outcomes. A significant increase in the number of infiltrating CD4+ cells (p=0.008) and a decrease in programmed death-1–positive (PD-1+) cells (p=0.032) were observed in the response patients.Conclusion In advanced BTC patients, nab-paclitaxel plus gemcitabine and cisplatin regimen showed therapeutic potential. Potential prognostic factors of CEA levels, number of CD4+ cells and PD-1+ cells may help us maximize the efficacy benefit.

Recent Progress on Corrosion Behavior and Mechanism of Mg–RE Based Alloys with Long Period Stacking Ordered Structure

Lisha Wang,Jinghua Jiang,Ting Yuan,Qiuyuan Xie,Huan Liu,Aibin Ma 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.5

Mg alloys containing long period stacking ordered (LPSO) structures possess excellent mechanical properties and corrosionresistance, exhibiting great application potential in biodegradable implants. The corrosion process of different LPSOphases in the Mg alloys need to be furtherly clarified in different solution environments. In this paper, we mainly reviewedthe influencing factors on the corrosion behavior of LPSO-containing Mg alloys, including the alloying elements, processingtechnologies, and the types, volume fractions and distributions of LPSO phases. Recent researches are well summarized withan emphasis on their corrosion mechanism. Special attention is given to the key issues for LPSO-containing Mg alloys asbiomedical implants, with their biodegradation behavior comprehensively discussed. The motivation is to provide theoreticalsupport for the possible application of LPSO-containing Mg alloys in the biomedical field.

Optimization of the Experimental Parameters Affecting the Corrosion Behavior for Mg–Y–Zn–Mn Alloy via Response Surface Methodology

Lisha Wang,Jinghua Jiang,Bassiouny Saleh,Reham Fathi,He Huang,Huan Liu,Aibin Ma 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

This paper optimizes experimental parameters on controlling corrosion performance of the Mg-rare-earth alloys with longperiod stacking ordered structure using response surface methodology. Different NaCl concentrations, temperatures, andvarying pH values are selected as input parameters. The corrosion current density (icorr) and open circuit potential (OCP)are used as the multiple responses to evaluate the corrosion performances. The corrosion surface morphology and corrosionmechanism of the Mg alloys are also investigated by scanning electron microscopy for the optimization. Analysis of variance(ANOVA) has given the impact of individual factors and interactions on the corrosion rate. The results indicated thatthe three test parameters had significant impacts in controlling the corrosion behavior of Mg alloy. Moreover, the increasedNaCl concentration decreased the pitting potential (Epit) of the target materials. Filiform corrosion can be detected in high pHsolutions, whereas the matrix suffered from severe dissolution phenomenon in low pH solutions. High temperature aggravatedthe local destruction and dissolution of the protective film. The interaction of the experimental parameters showed a sizableinfluence on corrosion performance.

Effect of Nano-CaCO3 on the Sealing Efficiency of Grouts in Flowing Water Grouting

Yao-Hui Liu,Ping Yang,Ze-Bin Ouyang,Xiao Wei,Lisha Zhang 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.10

Flowing water grouting is a big challenge in the tunneling and underground engineering. To enhance the early strength and grouting effectiveness of slurry for flowing water grouting, nano-CaCO3 and fly ash were mixed with cement based grout. A series of physical simulation tests were conducted to simulate the flowing water grouting process in rough rock fracture, and investigate the effect of nano-CaCO3 content on the fluid pressure and sealing efficiency of grouts. Results of viscosity tests show that the viscosity of grouts decreased with an increase of nano-CaCO3 content. Scanning electron microscope (SEM) tests indicate that nano-CaCO3can promote the formation of fibrous hydrates and enhance the flowing water resistance of grouts. Increasing nano-CaCO3 content resulted in the first increase while later decrease of maximal fluid pressure (MFP) and sealing efficiency (SE) of grouts. Reducing water cement ratio of grouts and incorporating fly ash can effectively improve the SE of flowing water grouting.

Numerical study on the cavitating flow through poppet valves concerning the influence of flow instability on cavitation dynamics

Cong Yuan,Lisha Zhu,Shiqi Liu,Du Zunling,He Li 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.2

The flow dynamics of the cavitating jet through poppet valves inevitably suffers from the influence of varying openness, but the relevant mechanism remains unclear. Hence, the present study conducts a numerical study on the cavitating flow phenomenon inside poppet valves with two valve seat structures, in order to examine the flow mechanisms underlying varying cavitation phenomenon at different openness. According to the results, the overall cavitation distribution as well as the morphology feature follows a similar pattern due to the same inception mechanism irrelevant of the openness amount. However, examination of the dynamic behaviors confirms discernable variation in 2 coupling effects between flow instability and cavitation. The flow separation at the poppet trailing edge produces non-cavitation flow instability at 0.4 mm openness and attached cavitation at 0.8 mm, which amalgamate with upstream-shed vortex cavitation. The stably attached cavitation within the chamfered groove at 0.4 mm, as the source of non-cavitation flow instability, and the detached cavitation at 0.6 and 0.8 mm openness produce vortex cavitation at the free shearing side. The substantial variation in these two coupling effects contributes sensibly to the increase in the overall cavitation intensity. Meanwhile, the weakened coupling effect between the free shearing vortex and wall shearing vortex, as a consequence of increased potential core thickness at larger openness, leads to the attenuated vortex cavitation at the wall poppet side. Additionally, the vortex cavitation at free shearing side has a larger size in the chamfered valve seat case, due to the coupling of shed flow instability with the Kelvin-Helmholtz instability. As a conclusion, the inception mechanism for the cavitation remains unchanged with openness, while the difference in flow instabilities as well as the associated coupling effects with cavitation contributes to the variation in cavitation intensity.

Illumina-sequencing based transcriptome study of coat color phenotypes in domestic goats

Yongdong Peng,Xiaohui Liu,Liying Geng,Ruxue Ma,Lisha Li,Jingshi Li,Chuansheng Zhang,Zhengzhu Liu,Yuanfang Gong,Xianglong Li 한국유전학회 2017 Genes & Genomics Vol.39 No.8

This study performed a comprehensive expression profiling of genes expressed in the skin of goats with three different coat colors by Illumina Sequencing. A total of 91 significantly expressed genes were detected when comparing gray skin to white skin library and these included 74 up-regulated and 17 down-regulated genes in gray skin. There were 67 differentially expressed genes between brown skin and white skin libraries, 23 of which were up-regulated and 44 were down-regulated in brown skin. When we compared brown and gray libraries, 154 differentially expressed genes were found, of which 33 showed higher expression and 121 showed lower expression in brown skin. To our surprise, MC1R, MITF, TYR and KIT showed no significant difference in expression between the goats with three skin colors, whereas ASIP was detected in white skin but not in dark skins. In this study, PMEL, TRPM1, TYRP1 and DCT were significantly upregulated in brown goat skin compare with gray and white skins. PMEL showed higher expression in gray goat skin compared with white goat skin, whereas there were no significant differences in the expression of TYRP1, TRPM1 and DCT between gray and white skin samples. In addition, ELOVL3 showed higher expression in gray goat skin than in brown and white skins, whereas there was no significant differences in the expression of ELOVL3 between brown and white skin samples. These results expand our understanding of the complex molecular mechanisms of skin physiology and melanogenesis in goat and provide a foundation for future studies.

Equiatomic, Cu‐Containing CrCuFeTiV 3d Transition Metal High Entropy Alloy with an Enhanced Strength and Hardness Synergy

Jiaojiao Yi,Lin Yang,Lu Wang,Mingqin Xu,Lisha Liu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.1

A novel 3d transition metal high entropy alloy (TM HEA), CrCuFeTiV, was fabricated by arc-melting under a vacuum condition. Its phase component, microstructure, and compressive properties in the as-cast and annealed conditions were carefullyinvestigated. The experimental results showed that the alloys in both conditions consist of one BCC phase (CrV/FeTi-richregions), one FCC phase (Cu-rich), and one hexagonal Laves phase (Cr2Ti). It is suggested that the CrV-rich BCC phasepreferentially separates as dendritic centers. Subsequently, the Cr2Ti Laves phase and the FeTi-rich BCC phase solidify as adendritic outer layer. Finally, the FCC Cu-rich phase is squashed into the remaining interdendritic regions. Besides, the yieldstrength, ultimate strength, and hardness of the as-cast CrCuFeTiV alloy are 1686 MPa, 2205 MPa, and 624 HV, while thosefor the annealed alloy are 1510 MPa, 2035 MPa, and 618 HV, respectively. The obtained values are the highest among thetabulated fve-principle equiatomic Cu-containing 3d TM HEAs. The promising strength-hardness synergy of CrCuFeTiValloy is likely originated from the majority constitution of the BCC phase and Cr2Ti Laves phase.

Epithelial CST1 Promotes Airway Eosinophilic Inflammation in Asthma via the AKT Signaling Pathway

Du Lijuan,Xu Changyi,Tang Kun,Shi Jia,Tang Lu,Lisha Xiao,Lei Chengcheng,Liu Huicong,Liang Yuxia,Guo Yubiao 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.3

Purpose: Epithelial cystatin SN (CST1), a type 2 cysteine protease inhibitor, was significantly upregulated in asthma. In this study, we aimed to investigate the potential role and mechanism of CST1 in eosinophilic inflammation in asthma. Methods: Bioinformatics analysis on Gene Expression Omnibus datasets were used to explore the expression of CST1 in asthma. Sputum samples were collected from 76 asthmatics and 22 control subjects. CST1 mRNA and protein expression in the induced sputum were measured by real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and western blotting. The possible function of CST1 was explored in ovalbumin (OVA)-induced eosinophilic asthma. Transcriptome sequencing (RNA-seq) was used to predict the possible regulated mechanism of CST1 in bronchial epithelial cells. Overexpression or knockdown of CST1 was further used to verify potential mechanisms in bronchial epithelial cells. Results: CST1 expression was significantly increased in the epithelial cells and induced sputum of asthma. Increased CST1 was significantly associated with eosinophilic indicators and T helper cytokines. CST1 aggravated airway eosinophilic inflammation in the OVA-induced asthma model. In addition, overexpression of CST1 significantly enhanced the phosphorylation of AKT and the expression of serpin peptidase inhibitor, clade B, member 2 (SERPINB2), while knockdown using anti-CST1 siRNA reversed the trend. Furthermore, AKT had a positive effect on SERPINB2 expression. Conclusions: Increased sputum CST1 may play a key role in the pathogenesis of asthma through involvement in eosinophilic and type 2 inflammation through activation of the AKT signaling pathway, further promoting SERPINB2 expression. Therefore, targeting CST1 might be of therapeutic value in treating asthma with severe and eosinophilic phenotypes.