둥글레(Polygonatum odoratum) 재분획물이 streptozotocin 유발 당뇨 흰쥐의 인슐린 활성에 미치는 영향 및 급성 독성에 관한 연구

임숙자,윤진선 德成女子大學校 1996 德成女大論文集 Vol.27 No.-

This study was carried out to investigate the hypoglycemic effect of subfractions from BuOH fraction in streptozotocin(STZ)-induced diabetic rats. Sixty male Sprague-Dawley diabetic rats weighing 200-300g were induced diabetes mellitus by the STZ injection(45mg/kg) into the tail vein and were devided into five groups; STZ-congrol, and four experimental groups(subfraction A, B, C and D). All groups were orally administered each subfraction, and normal and STZ-control groups were orally administered each subfraction, and normal and STZ-control groups were orally administered Tween 80 with the same amounts for 14 days. The body weight gain was monitored and the blood levels of glucose and cholesterol were analyzed. The levels of plasma triglyceride, HDL-cholesterol, free fatty acid and aminotransferase activity were determined. The extent of blood glucose decremints in rat of orally administered with subfractions was greater than that found in the STZ-control group. The plasma cholesterol levels were not influenced. The levels of insulin were higher in the subfraction groups compare to STZ-control group. The acute toxicity of BuOH fraction was not observed in the subfraction-administered mouse(5g/kg). Administration of subfractions significantly reduced the blood glucose level, AST/AlT activity and insulin showing the possibility of preventive and therapeutic use to diabetes mellitus

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_2 at 37℃. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KCI, 60 mM HEPES, pH 7.9, 25 mM MgCI_2, 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2 ㎍ of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_(2) at 37°C. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KC1, 60 mM HEPES, pH 7.9, 25 mM MgCl_(2), 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2μg of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

브랜드 인지도와 광고가 콘택트렌즈의 착용감과 가격만족도에 미치는 영향

임동규 ; 신상준 ; 한지선 ; 이익한 ; 김진숙 김천대학교 2016 김천대학교 논문집 Vol.36 No.-

The purpose of this research was to analyze the effects of brand awareness and brand advertising on price satisfaction and comfort in contact lens. We did a survey with 200 university students (85 male, 115 female) who have experience of wearing contact lens from April to May in 2015. The brand awareness of contact lens influences to purchasing intention of contact lens, it effects women more than men. Many respondents(53%) replied that the reliability is the most important elements of the advertising. This survey indicates that there is no statistically significant correlation between the brand awareness and advertisement with price satisfaction in contact lens. Therefore we could confirm they have dissatisfaction for prices of contact lens regardless to brand awareness or advertisement. The brand and advertising dependence group have more comfort of contact lens than non-dependence group when they are long time wear of contact lens. Therefore, there is statistically significant correlation between the brand awareness and advertisement with comfort of contact lens. As a result, brand awareness and reliability of advertising influences to purchasing intention of contact lens. But there is no statistically significant correlation with price satisfaction in contact lens. Whereas, brand awareness and advertisement have a statistically significant correlation with comfort of contact lens.

심정지환자에서 epinephrine투여시 혈중 catecholamine의 변화

임경수,황성오,이부수,이강현,이진웅,임종천,김영식,김선만,김 현 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Cardiac arrest is a potent stimulus for the release of endogenous catecholamine and high plasma catecholamine concentration has been reported during cardiopulmonary resuscitation(CPR). Few data were present about the clinical effect of high plasma catecholamine and endogenous catecholamine response to exogenous epinephrine administration during CPR. Purpose : This study was designed to evaluate endogenous catecholamine response to cardiac arrest and exogenously administered epinephrine during CPR. Method : In 21 cardiac arrest victims, blood sample for epinephrine and norepinephrine was drawn before and 1 minute after each epinephrine administration during CPR. 1mg of epinephrine was administered immediately after intravenous access and 3 minute interval during CPR. Plasma catecholamines were quantitated by high-performance liquid chromatography. Result : Baseline plasma epinephrine concentraqtion was higher in patients with cardiac arrest than normal controls (985±1627 vs 194±173 pg/ml, p<0.05). Baseline plasma norepinephrine concentration was also elevated in patients with cardiac arrest than normal controls, although statistically insignificnat(1965±4915 vs 360±250 pg/ml). Plasma epinephrine concentration in cardiac arrest patients was significantly elevated after exogenous administration of epinephrine, but its response was blunted after the third does of epinephrine. Plasma norepinephrine concentration in cardiac arrest patients was also elevated, but its response was observed only after the first epinephrine administration. Plasma epinephrine concentration was persistently higher in patients with return of spontaneous circulation (ROSC) than patients without ROSC. there was no significant difference of plasma norepinephrine concentration whether spontaneous circulation was restored or not. Endogenous epinephrine response to exogenous epinephrine was not observed in patients having prolonged arrest time over 20 minutes. Conclusion : Results of this study suggests that significant amount of catecholamine is released by exogenous epinephrine administration in spite of high plasma catecholamine concentration, and degree of endogenous catecholamine response influences resuscitation outcome.

잇솔질 압력에 따른 잇솔강모의 탄력손실도 측정

임선아,서은주,강성귀,성진효 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2

To investigate the elasticity loss of toothbrush according to the strength of pressure. the shape of brushing plane and the duration of use, thirty toothbrushes of five models were used for this examination among the toothbrushes for adults which have been marketed in the Republic of Korea. After 200g/㎠. 300g/㎠, or 400g/㎠ for 1, 2 or 3 month were loaded on toothbrushes, in rolling method the effect of the duration and strength of pressure and the change of the shape of brushing plane on the elasticity loss of toothbrushes was determined using Toothbrush Stroke Testen(CHUMDAN SYSTEM, Korea). The results are as follows; 1. The elasticity loss of toothbrushes according to 200, 300, or 400g/㎠ pressure was 11.11%, 25.21%, 30.84%, respectively. The elasticity loss of toothbrushes was increased with increasing the strength of the pressure (p<0.001) 2. The elasticity loss of the toothbrushes according to 1, 2, or 3months of the duration of use was in 15.60%, 22.09%, 29.47%, respectively. The elasticity loss of the toothbrushes was increased with increasing the duration of the pressure(p<0.001). 3. The elasticity loss of toothbrushes of convex plane according to 1, 2 or 3 month of the durate of use was 10.36% 18.29% and 24.20% respectively, that of toothbrushes of flat brushing plane according to 1, 2 or 3 months of the duration was 20.08%. 25.87% and 34.74%. respectively. The elasticity loss of the toothbrushes or flat brushing plane was higer than that of convex brushing plane(p<0.05). These results show that the property of the KS is optimal according to the pressure of toothbrushing, the shape of toothbrushos and the duration of toothbrushes using must be checked, and the further studies are needed about that.

박물관 관람자의 동선특성에 관한 조사

임채진,한선영 홍익대학교 산업디자인 연구소 1999 미술디자인 논문집 Vol.- No.4

The purpose of this study is to analyze the characteristics of exhibition circulation through the movement pattern. It looks into the factors under the influence with the visitor's movement. The method of analysis is statistics of investigation by following after visitors. The viewing behavioral of exhibition based on the time, speed, proportion of appreciation is classified into three types of the profound, the general, the passing viewers. The characteristics of the viewing form of these patterns largely depend on the space elements which are the structure form. And particularly the position of exit, the width of exhibition space and the connection of exhibition space stand out the disparity of the factor of the spatial structure. These three patterns bring about a lot of encounter in the exhibition space according to the difference of these routes consequently. So appropriate to the planning of each pattern can offer visitors to more effective environment of exhibition.

담즙정체성 간염의 임상적 양상

최선택,은종렬,임상우,김봉준,이헌주,구미진,최준혁 영남대학교 기초/임상의학연구소 2001 Yeungnam University Journal of Medicine Vol.18 No.1

Background: Cholestatic hepatitis is failure of bile to reach the duodenum with hepatocellular damage and no demonstrable obstruction of the major bile ducts. The prognosis is usually good with recovery in less than 4 weeks after withdrawal of the offending drug. However, a prolonged course lasting over 3 months is possible and, in rare cases, progression to ductopenia with development of a vanishing bile duct syndrome occurs. A differential diagnosis with other causes of Chronic liver disease is needed. Materials and Methods: From January 1991 through Jaunary 2000, 14 patients diagnosed as cholestatic hepatitis by liver biopsy were inclouded. The possible causative drug, clinical features, laboatory findings, and progression of cholestatic hepatitis were evaluated. The semiquantitative study of liver lesions was performed by two independent observers. Results: Causes of cholestatic hepatitis are 5 cases of oriental medicine, 3 cases of anti-tuberculosis medication, 1 case of ticlopidine and antibiotics and 4 cases of unknown causes. The clinical features of cholestatic hepatitis were jaundice, itching, urine color change, and general weakness. During 6 to 30 months, LFT of 5 patients showed prolonged elevation. Elevated total cholesterol ≥250 mg/dL in 6 patients, pheripheral blood eosinophilia in 5 patients, auto-antibody positive in 6 patients were observed respectively. The biopsies showed intralobular bilirubinostasis with a mixed portal inflammatory infiltration. Conclusion: In cholestatic hepatitis. durations of abnormal LFT are variable regardless of causative drugs. If cholestatic hepatitis progresses toward chronic course, viral hepatitis, primary biliary cirrhosis, and autoimmune hepatitis should be differentially diagnosed and sequential liver biopsies are needed.

한국 회나무(Euonymus planipes), 참회나무(E. oxyphyllus)와 나래회나무(E. macropterus)의 잎의 형질비교

장계선,장진성,임효인,류진아 서울大學校農業生命科學大學附屬樹木園 2008 서울大學校 樹木園 硏究報告 Vol.- No.26

한반도에 분포하는 참회나무, 회나무, 나래회나무의 영양기관에서의 유효 식별 형질을 확인하기 위하여 166점의 표본 조사를 통해 단변량 분석을 실시하였다. 3종을 대상으로 4개의 정량형질(잎의 길이, 잎의 너비, 엽두의 길이, 엽병의 길이)을 측정하였다. 형질 분석 결과 3종에 대한 잎의 형질은 종간 차이를 보이지 않아 종간 식별에 도움이 되지는 않았다. 따라서 본 분류군에 있어서 잎의 형질만을 가지고 종을 식별하는 것은 타당하지 않고 반드시 꽃이나 열매가 있어야 이 분류군을 인식할 수 있다고 판단된다. 분포에 있어서 회나무는 강원도에서 평안도에 걸쳐 분포하고, 참회나무는 백두대간을 중심으로 한반도 북부까지 분포하며 나래회나무는 한반도 남쪽지역을 중심으로 분포하는 양상으로 보여 분류군 간에 다소 차이가 있지만 대부분 강원도를 중심으로 분포가 겹친다. Leaves of 166 specimens were sampled to investigate patterns of interspecific variation and to evaluate recognition of Euonymus planipes, E. oxyphyllus. and E. macropterus using four leaf characters (length, width, apex length, and petiole length). This study suggested that these leaf characters were not useful to identify these taxa. Therefore, it is only possible to identify these closely related taxa based on flower and fruit characters unlike other horticulturists' view.

Inhibitory Effect of Clavicepitaceae on Serotonin Release out of Human Platelets and Human Platelet Aggregation

박화진,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

We have investigated the effects of hypha-water extracts (HWE), fruit body-water extracts (FWE) and cordycepin from Cordyceps militaris on serotonin release out of human platelets and human platelet aggregation. HWE and FWE inhibited the release of [^(3)H]-serotonin from human platelet stimulated by thrombin (2 U/ml) or collagen (20 ㎍/ml) in a dose-dependent manner. Furthermore, cordycepin, a major component of Cordyceps militaris, inhibited the human platelet aggregation induced by collagen (10 ㎍/ml) in a dose-dependent manner. These results suggest that cordycepin containing in HWE and FWE may inhibit the serotonin release by suppressing the collagen-induced human platelet aggregation. Accordingly, our data demonstrate that HWE and FWE containing much cordycepin might have antithrombotic and antimigrainous functions.