Synthesis and Electrochemical Performance of Ti3C2Tx with Hydrothermal Process

Libo Wang,Heng Zhang,Bo Wang,Changjie Shen,Chuanxiang Zhang,Qianku Hu,Aiguo Zhou,Baozhong Liu 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.5

In this study, a simple hydrothermal method has been developedto prepare Ti3C2Tx from Ti3AlC2 as a high-performance electrodematerial for supercapacitors. This method is environmentallyfriendly and has a low level of danger. The morphology andstructure of the Ti3C2Tx can be controlled by hydrothermalreaction time, temperature and NH4F amounts. The preparedTi3C2Tx was characterized by X-ray diffraction, field emissionscanning electron microscopy, Raman spectroscopy, X-rayphotoelectron spectroscopy and Brunauer-Emmet-Teller. Theresults show that the prepared Ti3C2Tx is terminated by O, OH,and F groups. The electrochemical properties of the Ti3C2Txsample exhibit specific capacitance up to 141 Fcm−3 in 3 MKOH aqueous electrolyte, and even after 1000 cycles, nosignificant degradation of the volumetric capacitance wasobserved. These results indicate that the Ti3C2Tx materialprepared by this hydrothermal method can be used in highperformance supercapacitors.

An Improved Method for Rapid Preparation of Oligodendrocytespecific Rabbit Polyclonal Antibody

Tao Wang,Wei Wang,Lintao Jia,Xin Bu,Fang Wang,Libo Yao,Bin Zhang 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3

Antibodies are important tools in the study of protein function and diagnostic tests. However, traditional antiserum preparation requires a time-consuming immunization protocol and subsequent purification of polyclonal antibodies. In this study, a rapid and efficient method for polyclonal antibody preparation has been developed. Juxtanodin (JN) and silent information regulator-2 (Sirt2),both of which are oligodendrocyte-specific proteins, were used for antibody preparation. The N-terminal 170 amino acids of JN (JN170) and amino acids 231-351 of Sirt2 (Sirt2-121) were expressed as GST-tagged proteins from a pET-41a(+) vector in E. coli strain BL21 (DE3) cells. The fusion proteins were purified and used to immunize rabbits following both a traditional protocol, in which antigen was presented biweekly, and a modified rapid protocol, in which the immunization on day 1 was boosted on days 5and 28. ELISA, Western blot analysis and immunofluorescent staining showed that antibodies produced via the rapid protocol could recognize these two oligodendrocytespecific proteins in vitro and in the rat central nervous system (CNS), respectively, similar to those produced with the traditional protocol. Thus, our study provides a novel rapid method to prepare high specificity antibodies via a modified immunization protocol and subsequent antibody purification.

An ultra-high pressure sensor with cylinder structure

Libo Zhao,Xin Guo,Xiawei Meng,Rahman Hebibul,Yulong Zhao,Jianzhu Wang,Zhuangde Jiang 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.8

Based on strain-resistance effect, an ultra-high pressure sensor has been developed with the range of 0-500 MPa. It is mainly composed of the elastic element and sensitive element. This sensor’s range is determined by the elastic element’s structure and material. In order to endure the ultra-high pressure, the elastic element’s material is selected as spring steel, and its structures are analyzed and simulated. After that, the metal strain gauge as the sensitive element is packaged on the elastic element. Then the sensor is developed after some compensation processes are carried out. In the end, the sensor is calibrated. The results show that the sensor has good performance,such as the accuracy of 0.35%FS, which meets the requirements of aerospace, weapons and other fields.

The Effect of Magnetic Field Direction on the Imaging Quality of Scanning Electron Microscope

Libo Ai,Shengxiang Bao,Yongda Hu,Xueke Wang,Chuan Luo 한국자기학회 2017 Journal of Magnetics Vol.22 No.1

The significant reduction of the image quality caused by the magnetic field of samples is a major problem affecting the application of SEM (scanning electron microscopy) in the analysis of electronic devices. The main reason for this is that the electron trajectory is deflected by the Lorentz force. The usual solution to this problem is degaussing the sample at high temperatures. However, due to the poor heat resistance of some electronic components, it is imperative to find a method that can reduce the impact of magnetic field on the image quality and is straightforward and easy to operate without destroying the sample. In this paper, the influence of different magnetic field directions on the imaging quality was discussed by combining the experiment and software simulation. The principle of the method was studied, and the best observation direction was obtained.

Isolation and characterization of a novel piezotolerant bacterium Lysinibacillus yapensis sp. nov., from deep-sea sediment of the Yap Trench, Pacific Ocean

Libo Yu,Xixiang Tang,Shiping Wei,Yinkun Qiu,Xiashutong Xu,Guangxin Xu,Qilin Wang,Qian Yang 한국미생물학회 2019 The journal of microbiology Vol.57 No.7

A Gram-positive, aerobic, rod-shaped, spore-forming bacterium, designated YLB-03T, with peritrichous flagella was isolated from deep-sea sediment of the Yap Trench at a depth of 4435 m. The bacterium was found to be catalase-positive but oxidase-negative. Growth of this bacterium was observed at 15–50°C (optimum 37°C), pH 5–10.5 (optimum 7), 0–5% NaCl (optimum 1%, w/v) and 0.1–50 MPa (optimum 0.1 MPa). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YLB-03T was a member of the genus Lysinibacillus. Strain YLB-03T was closely related to Lysinibacillus sinduriensis BLB-1T and Lysinibacillus chungkukjangi 2RL3-2T (98.4%), Lysinibacillus halotolerans LAM- 612T (98.0%), Lysinibacillus telephonicus KT735049T (97.5%), Lysinibacillus endophyticus C9T (97.5%), Lysinibacillus composti NCCP-36T and Lysinibacillus massiliensis 4400831T (97.3%). The ANI and the GGDC DNA-DNA hybridization estimate values between strain YLB-03T and closely related type strains were 73.7–76.3% and 34.7–38.7%, respectively. The principal fatty acids were anteiso-C15:0 and iso-C15:0. The G+C content of the chromosomal DNA was 39.6 mol%. The respiratory quinone was determined to be MK-7. The diagnostic amino acids in the cell wall peptidoglycan contained Lys-Asp (type A4α) and the cell-wall sugars were glucose and xylose. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid. The combined genotypic and phenotypic data showed that strain YLB-03T represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus yapensis sp. nov. is proposed, with the type strain YLB-03T (= MCCC 1A12698T = JCM 32871T).

Research on Control Force Aerodynamic Model of a Guided Rocket With an Isolated-rotating Tail Rudder

Chen Wang,Libo Ding,He Zhang,Li Xiao,Shihao Zhang 한국항공우주학회 2022 International Journal of Aeronautical and Space Sc Vol.23 No.1

Isolated rotating tail rudder technology provides a low-cost and miniaturized solution for the correction and guidance of a man-portable rocket. The results of three turbulence models (the Spalart–Allmaras model, standard k–ε model, and shear stress transport k–ω model) were compared with wind-tunnel model test data, and the best turbulence model was selected. An aerodynamic model of the rotating tail rudder was developed by identifying its turbulent region, and the influences of the Mach number, angle of attack, and tail rudder speed on the projectile aerodynamics were revealed. The aerodynamic parameters were fitted using a least-squares method, and the vector variation characteristics of the period-averaged control force were analyzed. The results from the shear stress transport k–ω model were closest to the results of the wind-tunnel tests. The aerodynamic model was able to fit the simulation results well. The average control force of the tail rudder over a rotation cycle is not zero, and it increases with increasing angle of attack, Mach number, and tail rudder rotation speed. This study provides a basis for aerodynamic research examining the same type of projectile, and it has guiding significance for the control design of isolated rotating tail rockets.

Function identification of bovine Nramp1 promoter and intron 1

Hao, Linlin,Zhang, Libo,Li, Mingtang,Nan, Wang,Liu, Songcai,Zhong, Jifeng The Korean Society for Integrative Biology 2011 Animal cells and systems Vol.15 No.3

The Nramp1/Slc11a1 locus encodes a proton-coupled divalent cation transporter, expressed in late endosomes/lysosomes of macrophages, that constitutes a component of the innate immune response to combat intracellular pathogens and it was shown to play an important role in regulating inherent immunity. The previously identified Z-DNA forming polymorphic repeat(GT)n in the promoter region of the human Nramp1 gene does act as a functional polymorphism influencing gene expression. Research has shown that INF-${\gamma}$, TNF-${\alpha}$, IL-$1{\beta}$ and bacteria LPS increase the level of Nramp1 expression. However, the molecular mechanism for Nramp1 gene regulation is unclear. In this research, bovine Nramp1 5'-flanking region (-1748~+769) was cloned and analyzed by bioinformatics. Then to find the core promoter and the cis-acting elements, deletion analysis of promoter was performed using a set of luciferase reporter gene constructs containing successive deletions of the bovine Nramp1 5'-flanking regions. Promoter activity analysis by the dual luciferase reporter assay system showed that the core promoter of Nramp1 was located at +58~-89 bp. Some positive regulatory elements are located at -89~-205 bp and -278~-1495 bp. And the repressor elements were in region -205~-278 bp, intron1 and -1495~-1748 bp. LPS-responsive regions were located at -1495~-1748 bp and -278~-205 bp. The present study provides an initial effort to explore the molecular mechanism of transcriptional activation of the bovine Nramp1 gene and should facilitate further studies to decode the complex regulatory process and for molecular breeding for disease resistance in bovines.

Analysis of Risk Factors for a Poor Prognosis in Patients with Anti-N-Methyl-D-Aspartate Receptor Encephalitis and Construction of a Prognostic Composite Score

Yejia Mo,Li Wang,Libo Zhu,Feng Li,Gang Yu,Yetao Luo,Meng Ni 대한신경과학회 2020 Journal of Clinical Neurology Vol.16 No.3

Background and Purpose Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is the most-common form of autoimmune encephalitis, but its early diagnosis is challenging. This study aimed to identify the risk factors for a poor prognosis in anti-NMDAR encephalitis and construct a prognostic composite score for obtaining earlier predictions of a poor prognosis. Methods We retrospectively analyzed the clinical data, laboratory indexes, imaging findings, and electroencephalogram (EEG) data of 60 patients with anti-NMDAR encephalitis. The modified Rankin Scale (mRS) scores of patients were collected when they were discharged from the hospital. The mRS scores were used to divide the patients into two groups, with mRS scores of 3–6 defined as a poor prognosis. Logistic regression analysis was used to analyze independent risk factors related to a poor prognosis. Results This study found that 23 (38.3%) and 37 (61.7%) patients had good and poor prognoses, respectively. Logistic regression analysis showed that age, disturbance of consciousness at admission, and ≥50% slow waves on the EEG were significantly associated with patient outcomes. An age, consciousness, and slow waves (ACS) composite score was constructed to predict the prognosis of patients with anti-NMDAR encephalitis at an early stage based on regression coefficients. Conclusions Age, disturbance of consciousness at admission, and ≥50% slow waves on the EEG were independent risk factors for a poor prognosis. The ACS prognostic composite score could play a role in facilitating early predictions of the prognosis of anti-NMDAR encephalitis.

RhGLP-1 (7-36) protects diabetic rats against cerebral ischemia-reperfusion injury via up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD

Yi Fang,Xiaofang Liu,Libo Zhao,Zhongna Wei,Daoli Jiang,Hua Shao,Yannan Zang,Jia Xu,Qian Wang,Yang Liu,Ye Peng,Xiaoxing Yin 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5

The present study aimed to explore the neuroprotective effect and possible mechanisms of rhGLP-1 (7-36) against transient ischemia/reperfusion injuries induced by middle cerebral artery occlusion (MCAO) in type 2 diabetic rats. First, diabetic rats were established by a combination of a high-fat diet and low-dose streptozotocin (STZ) (30 mg/kg, intraperitoneally). Second, they were subjected to MCAO for 2 h, then treated with rhGLP-1 (7-36) (10, 20, 40 μg/kg i.p.) at the same time of reperfusion. In the following 3 days, they were injected with rhGLP-1 (7- 36) at the same dose and route for three times each day. After 72 h, hypoglycemic effects were assessed by blood glucose changes, and neuroprotective effects were evaluated by neurological deficits, infarct volume and histomorphology. Mechanisms were investigated by detecting the distribution and expression of the nuclear factor erythroid-derived factor 2 related factor 2 (Nrf2) in ischemic brain tissue, the levels of phospho-PI3 kinase (PI3K)/PI3K ratio and heme-oxygenase-1 (HO-l), as well as the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA). Our results showed that rhGLP-1 (7-36) significantly reduced blood glucose and infarction volume, alleviated neurological deficits, enhanced the density of surviving neurons and vascular proliferation. The nuclear positive cells ratio and expression of Nrf2, the levels of P-PI3K/PI3K ratio and HO-l increased, the activities of SOD increased and the contents of MDA decreased. The current results indicated the protective effect of rhGLP-1 (7-36) in diabetic rats following MCAO/R that may be concerned with reducing blood glucose, up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD.

Chemo-Mechanical Manufacturing of Fused Silica by Combining Ultrasonic Vibration with Fixed-Abrasive Pellets

Yaguo Li,Yongbo Wu,Libo Zhou,Masakazu Fujimoto,Jian Wang,Qiao Xu,Shoichi Sasaki,Masaaki Kemmochi 한국정밀공학회 2014 International Journal of Precision Engineering and Vol.15 No.5

Vibration-assisted grinding, in which harder abrasives than materials to be machined are employed, has been a viable and effective approach to increasing material removal rate (MRR) and/or reducing surface roughness of ground surfaces. We transfer this ideology to fused silica polishing by incorporating ultrasonic vibration into recently developed fixed-abrasive pellets in an attempt to enhance MRR and/or to improve manufactured surface quality. A prototype ultrasonic vibrator, the heart of the polishing head, was designed and the related experimental work was performed on an in-house built setup in conjunction with the constructed head. The vibrator is devised for the generation of 2-D tool path despite using only one actuator in lieu of two actuators in conventional 2-D ultrasonic machining systems. We then combined the ultrasonic vibration with fixed abrasive polishing pellets to machine fused silica glass. Machining experiments reveal that MRR is considerably increased up to >50% upon the introduction of ultrasonic vibration (UV) whilst surface roughness is not degraded appreciably. It was also noted that a overwhelmingly greater deal of polishing debris was dispelled during ultrasonic vibration assisted polishing than conventional bound-abrasive polishing, which may account for the greater MRR in UV assisted polishing.