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Lei Zhou,Xiao-Fei An,Shi-Chao Teng,Jing-Shun Liu,Wen-Bin Shang,Ai-Hua Zhang,Yang-Gang Yuan,Jiang-Yi Yu 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.5
Diabetic nephropathy (DN) is an important diabetic complication, and podocyte apoptosis plays a critical role in the development of DN. In the present study, we examined the preventive effect of the total flavone glycosides of Flos Abelmoschus manihot (TFA) on urinary microalbumin and glomerular podocyte apoptosis in experimental DN rats. The preliminary oral administration of TFA (200 mg/kg/day) for 24 weeks significantly decreased the urinary microalbumin to creatinine ratio and 24-h urinary total protein in streptozotocin-induced DN rats. Terminal deoxynucleotidyl transferasemediated dUTP nick end-labeling assay indicated glomerular cell apoptosis in DN rats was significantly improved by pretreatment with TFA. Furthermore, fluorescence-activated cell sorting and Hoechst 33342 staining suggested preincubation with hyperoside (50 and 200 lg/mL), the major active constituent of TFA, could significantly mitigate cultured podocyte apoptosis induced by the advanced glycation end-products (AGEs). Western blot analysis showed that increased caspase-3 and caspase-8 expressions induced by AGEs were also inhibited by pretreatment with hyperoside at both doses. Our results demonstrate that TFA pretreatment can decrease urinary albumin excretion in early-stage DN, which might be accomplished by preventing renal damage and podocyte apoptosis.
Upregulation of MicroRNA-34a Sensitizes Ovarian Cancer Cells to Resveratrol by Targeting Bcl-2
Shangli Yao,Ming Gao,Zujun Wang,Wenyan Wang,Lei Zhan,Bing Wei 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.8
Purpose: Resveratrol (REV), a natural compound found in red wine, exhibits antitumor activity in various cancers, includingovarian cancer (OC). However, its potential anti-tumor mechanisms in OC are not well characterized. Here, we tried to elucidatethe underlying mechanisms of REV in OC cells. Materials and Methods: The anti-proliferative effects of REV against OC cells were measured using CCK-8 assay. Apoptosis wasmeasured using an Annexin V-FITC/PI apoptosis detection kit. The anti-metastasis effects of REV were evaluated by invasion assayand wound healing assay. The miRNA profiles in REV-treated cells were determined by microarray assay. Results: Our results showed that REV treatment suppresses the proliferation, induces the apoptosis, and inhibits the invasion andmigration of OV-90 and SKOV-3 cells. miR-34a was selected for further study due to its tumor suppressive roles in various humancancers. We found miR-34a overexpression enhanced the inhibitory effects of REV on OC cells, whereas miR-34a inhibition hadthe opposite effect in OC cells. In addition, we verified that BCL2, an anti-apoptotic gene, was found directly targeted by miR-34a. We also found that REV reduced the expression of Bcl-2 in OC cells. Further investigations revealed that overexpression of Bcl-2significantly abolished the anti-tumor effects of REV on OC cells. Conclusion: Overall, these results demonstrated that REV exerts anti-cancer effects on OC cells through an miR-34a/Bcl-2 axis,highlighting the therapeutic potential of REV for treatment of OC.
Annealing Effects on CuInS2 Thin Films Grown on Glass Substrates by Using Pulsed Laser Deposition
Lei Zhang,Jingang Fang,Mingkai Li,Xunzhong Shang,Yunbin He,강태원 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.64 No.3
CuInS2 thin films have been deposited on glass substrates at low temperature by using a self-madeceramic target and the pulsed laser deposition method. Polycrystalline and near-stoichiometricCuInS2 films with a band gap energy of 1.45 eV are obtained by post-annealing/sulfurization ata temperature of 500 C. X-ray diffraction reveals a highly (112) preferential orientation of theCuInS2 thin films while the Raman spectrum indicates the coexistence of a CuInS2 chalcopyritephase and CuAu orderings. A polymorphic transformation of metastable CuAu ordering into theequilibrium chalcopyrite structure takes place at a post-annealing temperature of 500 C with theCuxS phase being segregated at the surface. Further, the morphologies and the chemical statesof the films before and after annealing are characterized by atomic force microscopy and X-rayphotoelectron spectroscopy, respectively.
Junliang Shang,Junying Zhang,Xiujuan Lei,Wenying Zhao,Yafei Dong 한국유전학회 2013 Genes & Genomics Vol.35 No.3
Epistasis is a ubiquitous phenomenon in genetics,and is considered to be one of the main factors in current efforts to detect missing heritability for complex diseases. Simulation is a critical tool in developing methodologies that can more effectively detect and study epistasis. Here we present a simulator, epiSIM (epistasis SIMulator), that can simulate some of the statistical properties of genetic data. EpiSIM is capable of expanding the range of the epistasis models that current simulators offer, including epistasis models that display marginal effects and those that display no marginal effects. One or more of these epistasis models can be embedded simultaneously into a single simulation data set, jointly determining the phenotype. In addition,epiSIM is independent of any outside data source in generating linkage disequilibrium patterns and haplotype blocks. We demonstrate the wide applicability of epiSIM by performing several data simulations, and examine its properties by comparing it with current representative simulators and by comparing the data that it generates with real data. Our experiments demonstrate that epiSIM is a valuable addition and a nice complement to the existing epistasis simulators. The software package is available online at https://sourceforge.net/projects/episimsimulator/files/.
Incorporating heuristic information into ant colony optimization for epistasis detection
Junliang Shang,Junying Zhang,Xiujuan Lei,Yuanyuan Zhang,Baodi Chen 한국유전학회 2012 Genes & Genomics Vol.34 No.3
Epistasis has been receiving increasing attention in understanding the mechanism underlying susceptibility to complex diseases. Though many works have been done for epistasis detection, genome-wide association study remains a challenging task: it makes the search space excessively huge while solution quality is excessively demanded. In this study, we introduce an ant colony optimization based algorithm,AntMiner, by incorporating heuristic information into ant-decision rules. The heuristic information is used to direct ants in the search process for improving computational efficiency and solution accuracy. During iterations, chi-squared test is conducted to measure the association between an interaction and the phenotype. At the completion of the iteration process, statistically significant epistatic interactions are ordered and then screened by a post-procedure. Experiments of AntMiner and its comparison with existing algorithms epiMODE, TEAM and AntEpiSeeker are performed on both simulation data sets and real age-related macular degeneration data set, under the criteria of detection power and sensitivity. Results demonstrate that AntMiner is promising for epistasis detection. In terms of detection power, AntMiner performs best among all the other algorithms on all cases regardless of epistasis models and single nucleotide polymorphism size; compared with AntEpiSeeker, AntMiner can obtain better detection power but with less ants and iterations. In terms of sensitivity, AntMiner is better than AntEpiSeeker in detecting epistasis models displaying marginal effects but it has moderate sensitivity on epistasis models displaying no marginal effects. The study may provide clues on heuristics for further epistasis detection. The software package is available online at https://sourceforge.net/projects/antminer/files/.
Zhanlei Shang,Jing Cheng,Junwei Lei,Ruiqi Wang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.6
Synchronization of chaotic systems can be used in the secure communication so it greatly attracted interests of many researchers. The bounded characteristics of chaotic system was used and an uniformed adaptive law was designed to solve system uncertainties which make the whole design very simple. And two kinds of functions was used to take place of traditional sign function, then the chattering problem was greatly improved. A Lyapunov function was chosen to guarantee the stability of the whole design. At last, detailed simulation was done to show the rightness and effectiveness of the proposed method.
Synchronization of Uncertain Chaotic System with Unknown Response System Parameters
Zhanlei Shang,Wenjun Song,Ruiqi Wang,Junwei Lei 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.4
Synchronization of chaotic system is meaningful and widely used in secure communication. A kind of robust adaptive synchronization method was proposed to solve a special chaotic synchronization problem that both response system and driven system have unknown parameters and uncertain nonlinear functions. And the difficulty is also caused by the different structures between master chaotic system and slave chaotic system. A kind of update law of estimation of unknown parameters were designed by choose a proper Lyapunov energy function. And the stability of the whole system is guaranteed by Lyapunov stability theorem. At last, detailed numerical simulation was done to show the rightness of the proposed method.