In-situ encapsulation of flaky aluminum pigment with poly (methylhydrosiloxane) anti-corrosion film for high-performance waterborne coatings

Chuanmin Zhang,Ran Huo,Xiaodong Wang,Junying Zhang,Jue Cheng,Ling Shi 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.89 No.-

To improve the corrosion resistance and glossiness offlaky aluminum pigments in waterborne coatings, aflaky aluminum pigment was encapsulated with poly(methylhydrosiloxane) (PMHS) as an anti-corrosionfilm by in-situ polymerization, and its chemical composition was characterized by Fourier-transforminfrared and X-ray photoelectron spectroscopy, and its morphology and microstructure was identified byscanning and transmission electron microcopy. Furthermore, its anticorrosive performance wasconfirmed by Zeta potential measurement and electrochemical impedance spectroscopy. Theencapsulatedflaky aluminum pigment not only exhibits excellent corrosion resistance but also showsan improved thermal stability due to the fabrication of a PMHS-based organosiliconfilm on the surface ofaluminumflakes. Most of all, the original glossiness and brightness could be maintained for thewaterborne coatingfilm after the encapsulatedflaky aluminum pigment was incorporated, indicating agreat application potential in waterborne coatings, water-based paints, printing inks, spray-freematerials and any more. This work provides a new strategy for design and fabrication of high corrosionresistantand high glossy aluminum pigments.

Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita

Wenjie Tong(Wenjie Tong),Junying Li(Junying Li),Wenfeng Cong(Wenfeng Cong),Cuiping Zhang(Cuiping Zhang),Zhaoli Xu(Zhaoli Xu),Xiaolong Chen(Xiaolong Chen),Min Yang(Min Yang),Jiani Liu(Jiani Liu),Lei Yu 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations.

Genome-wide identification of drought-responsive microRNAs and their target genes in Chinese jujube by deep sequencing

Zhang Luhe,Li Yi,Yang Jiangwei,Huang Huali,Lu Qian,Zhao Junying,Wang Fang,Wang Duofeng 한국유전학회 2023 Genes & Genomics Vol.45 No.2

Background MicroRNAs (miRNAs) are about 21 snucleotide (nt) long, non-coding RNAs that play an important role in plant abiotic stress responses. Chinese jujube is a native fruit tree in China, which is also an admittedly drought-resistant plant. But the drought-related miRNAs have little been reported in jujube. Objective To identify possibly drought-responsive microRNAs and their target genes in Chinese Jujube. Methods Twelve small RNA libraries were constructed from two jujube genotypes both drought treated and control samples with three replicates to identify known and novel miRNAs in Chinese Jujube, DESeq2 was used to identify expression pattern of miRNAs between drought treatment and control samples, TargetFinder program was used to predict potential target genes of conserved and novel miRNAs, RT-qPCR were used to analysis the expression levels of drought-related miRNAs and their potential targets. The RNA ligase-mediated RLM-5′ RACE experiments were performed to validate predicted target genes of drought-related miRNAs. Results 43 known miRNAs and 431 novel miRNAs were identified in Chinese jujube. Expression analysis showed that 28 miRNAs were differential expressed under drought stress in jujube variety “Dongzao”, including 21 up-regulated miRNAs and 7 down-regulated miRNAs, 61 miRNAs were differential expressed under drought stress in Chinese jujube variety “Zanhuangdazao”, including 23 up-regulated miRNAs and 37 down-regulated miRNAs. Depend on miRNAs target prediction, functional annotation and expression analysis, we identified 9 drought-related miRNAs, and 7 target genes of 6 miRNAs were confirmed using the modified 5′-RACE method. Also, RT-qPCR analyses revealed that relative expression of those miRNAs and their targets have negative tendency. Conclusion We identified 6 drought-related miRNAs by high-throughout sequencing and target gene annotation from Chinese jujube, and targets of those miRNAs were confirmed by the modified 5′-RACE method. These findings provide molecular evidence for enhancing drought tolerance in Chinese jujube and other plants.

Clinical Biomarkers of Neurodegeneration in REM Sleep Behavior Disorder

Junying Zhou,Jihui Zhang,Siu Ping Lam,Xiangdong Tang,Yun Kwok Wing 대한수면연구학회 2015 Journal of sleep medicine Vol.12 No.2

Rapid eye movement (REM) sleep behavior disorder (RBD) is currently considered as a prodromal stage of α -synucleinopathies neurodegeneration. The update data suggested that over 80% patients with idiopathic RBD eventually developed neurodegenerative disease after a mean of 14 years interval from the onset of RBD. A series of potential biomarkers have been identified to predict the development of neurodegeneration in idiopathic RBD, including olfactory loss, color vision deficit, depression, mild cognitive impairment, excessive daytime sleepiness, dopamine dysfunction, and tonic EMG activity. Early recognition of the predictive markers of neurodegeneration in idiopathic RBD is essential for development of intervention or prevention strategies at the presymptomatic stage. Nonetheless, the current literature is lacking biomarkers that might reflect the α-synuclein neuropathology at the earliest stages. Future studies with large samples and systematic follow-up are needed to confirm more potential markers of neurodegeneration at its early stages.

miRNA Pattern Discovery from Sequence Alignment

Xiaohan Sun,Junying Zhang 한국정보처리학회 2017 Journal of information processing systems Vol.13 No.6

MiRNA is a biological short sequence, which plays a crucial role in almost all important biological process. MiRNA patterns are common sequence segments of multiple mature miRNA sequences, and they are ofsignificance in identifying miRNAs due to the functional implication in miRNA patterns. In the proposedapproach, the primary miRNA patterns are produced from sequence alignment, and they are then cut intoshort segment miRNA patterns. From the segment miRNA patterns, the candidate miRNA patterns areselected based on estimated probability, and from which, the potential miRNA patterns are further selectedaccording to the classification performance between authentic and artificial miRNA sequences. Threeparameters are suggested that bi-nucleotides are employed to compute the estimated probability of segmentmiRNA patterns, and top 1% segment miRNA patterns of length four in the order of estimated probabilitiesare selected as potential miRNA patterns.

EpiSIM: simulation of multiple epistasis, linkage disequilibrium patterns and haplotype blocks for genome-wide interaction analysis

Junliang Shang,Junying Zhang,Xiujuan Lei,Wenying Zhao,Yafei Dong 한국유전학회 2013 Genes & Genomics Vol.35 No.3

Epistasis is a ubiquitous phenomenon in genetics,and is considered to be one of the main factors in current efforts to detect missing heritability for complex diseases. Simulation is a critical tool in developing methodologies that can more effectively detect and study epistasis. Here we present a simulator, epiSIM (epistasis SIMulator), that can simulate some of the statistical properties of genetic data. EpiSIM is capable of expanding the range of the epistasis models that current simulators offer, including epistasis models that display marginal effects and those that display no marginal effects. One or more of these epistasis models can be embedded simultaneously into a single simulation data set, jointly determining the phenotype. In addition,epiSIM is independent of any outside data source in generating linkage disequilibrium patterns and haplotype blocks. We demonstrate the wide applicability of epiSIM by performing several data simulations, and examine its properties by comparing it with current representative simulators and by comparing the data that it generates with real data. Our experiments demonstrate that epiSIM is a valuable addition and a nice complement to the existing epistasis simulators. The software package is available online at https://sourceforge.net/projects/episimsimulator/files/.

miRNA Pattern Discovery from Sequence Alignment

Sun, Xiaohan,Zhang, Junying Korea Information Processing Society 2017 Journal of information processing systems Vol.13 No.6

MiRNA is a biological short sequence, which plays a crucial role in almost all important biological process. MiRNA patterns are common sequence segments of multiple mature miRNA sequences, and they are of significance in identifying miRNAs due to the functional implication in miRNA patterns. In the proposed approach, the primary miRNA patterns are produced from sequence alignment, and they are then cut into short segment miRNA patterns. From the segment miRNA patterns, the candidate miRNA patterns are selected based on estimated probability, and from which, the potential miRNA patterns are further selected according to the classification performance between authentic and artificial miRNA sequences. Three parameters are suggested that bi-nucleotides are employed to compute the estimated probability of segment miRNA patterns, and top 1% segment miRNA patterns of length four in the order of estimated probabilities are selected as potential miRNA patterns.

As a Modulator, Multitasking Roles of SIRT1 in Respiratory Diseases

Zhou Yunxin,Zhang Fan,Ding Junying 대한면역학회 2022 Immune Network Vol.22 No.3

As far the current severe coronavirus disease 2019 (COVID-19), respiratory disease is still the biggest threat to human health. In addition, infectious respiratory diseases are particularly prominent. In addition to killing and clearing the infection pathogen directly, regulating the immune responses against the pathogens is also an important therapeutic modality. Sirtuins belong to NAD+-dependent class III histone deacetylases. Among 7 types of sirtuins, silent information regulator type-1 (SIRT1) played a multitasking role in modulating a wide range of physiological processes, including oxidative stress, inflammation, cell apoptosis, autophagy, antibacterial and antiviral functions. It showed a critical effect in regulating immune responses by deacetylation modification, especially through high-mobility group box 1 (HMGB1), a core molecule regulating the immune system. SIRT1 was associated with many respiratory diseases, including COVID-19 infection, bacterial pneumonia, tuberculosis, and so on. Here, we reviewed the latest research progress regarding the effects of SIRT1 on immune system in respiratory diseases. First, the structure and catalytic characteristics of SIRT1 were introduced. Next, the roles of SIRT1, and the mechanisms underlying the immune regulatory effect through HMGB1, as well as the specific activators/inhibitors of SIRT1, were elaborated. Finally, the multitasking roles of SIRT1 in several respiratory diseases were discussed separately. Taken together, this review implied that SIRT1 could serve as a promising specific therapeutic target for the treatment of respiratory diseases.

Mutual information and linkage disequilibrium based SNP association study by grouping case‐control

Xiguo Yuan,Junying Zhang,Yue Wang 한국유전학회 2011 Genes & Genomics Vol.33 No.1

Two main reasons for the difficulties to search for susceptibility single‐nucleotide polymorphisms (SNPs) underlying genetic diseases are that the findings are not easy to be confirmed and the interactions between potential susceptibility SNPs are not clear. Many available association studies usually presented results with significance levels but did not illustrate the stability of the results. In some sense, their performances might be unclear in real practice. In this paper, we develop a novel method based on mutual information theory and linkage disequilibrium by grouping case‐control. Mutual information (MI)is used to test multiple SNPs in combining with disease status. Those SNPs contributing the maximum MI are selected as potential susceptibility SNPs. Linkage disequilibrium (LD) analysis is used to extend MI detected result so that both direct and indirect factors can be included in the final result. The purpose of case‐control grouping is to generate a number of data groups by randomly sampling from target samples. Each group is assumed to have almost the same number of individuals (cases and controls), and overlap is allowed among the groups. We apply the method to each data group, and then make comparisons and intersections between the results obtained from each of the groups so as to give the final result. We implement the method by continuously grouping until the final result reaches a stable state and a highly significance level. The experimental results indicate that our method to detect susceptibility SNPs in simulated and real data sets has shown remarkable success.

Evaluating the electric property of different crystal faces and enhancing the Raman scattering of Cu2O microcrystal by depositing Ag on the surface

Hong Gao,Junying Zhang,Min Li,Kejia Liu,Dong Guo,Yue Zhang 한국물리학회 2013 Current Applied Physics Vol.13 No.5

The surface electric property of Cu2O microcrystal affects the interaction of facets with substance in the aqueous solution, and hence plays a key role in determining the photocatalytic activity. In this paper, the capability of Cu2O microcrystals with exclusive {111}, {110} or both lattice surfaces in reducing Agþ to Ag0were investigated. Ag particles selectively deposited on {111} surfaces of Cu2O, while not on {110} surfaces. The different behaviors of the two surfaces are mainly attributed to their different electric properties:negatively-charged {111} surfaces absorb Agþ ions while positively-charged {110} surfaces repel them. Raman scattering of Cu2O {111} surfaces was enhanced by the photo-deposition of Ag particles.