Effects of Cyclic AMP on the Activities of Ribosome and Elongation Factors EF-1 and EF-2

심웅섭,문혜연,Sim, Woong-Seop,Moon, Hye-Yeon 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.3

단백질의 생합성에 필요한 여러가지 components의 활성에 미치는 cAMP 의 효과를 조사하였다. cAMP로 처리된 ribosome의 활성은 대조구에 비하여 27% 증가하였으며 EF-1 과 EF-2의 활성은 cAMP에 의하며 각각 17%와 20% 증가 하였다. The effects of cAMP on the activities of the various components necessary for protein biosynthesis were investigated. 1. The rate of protein biosynthesis was increased about 27% by ribosomes treated with cAMP. 2. The activities of elongation factors EF-1 and EF-2 were considerably stimulated by exogenous cAMP.

Studies on the Mechanisms of Gibberellic Acid Action(III). Regulation of Protein Biosynthesis and Phosphorylation by cyclic AMP

심웅섭,노광수,문혜연,Sim, Woong-Seop,Roh, Kwang-Soo,Moon, Rye-Yeon 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.1

cAMP가 $GA_3$의 작용기작에 관여하는 지를 밝히기 위해 단백질의 생합성과 인산화반응에 미치는 cAMP의 효과를 구명하였던바, 정상상태에서의 단백질의 생합성 및 인산화 반응은 cAMP에 의해 모두 촉진되었다. Transcription이 억제된 상태에서는 단백질의 생합성은 전 발아기간 중 대조구에 비해 촉진되었으나 인산화 반응은 발아 3일 후 cAMP에 의해 증가되었다. 또한 cAMP는 발아 초기에는 단백질의 인산화 반응 보다는 생합성을 촉진시키고, 발아가 진행됨에 따라 단백질의 생합성 보다는 인산화 반응을 촉진시킨다. 이러한 결과는 단백질의 생합성 및 인산화반응에 미치는 $GA_3$의 촉진효과와 동일한 점으로 보아 cAMP는 $GA_3$의 작용기작과 밀접한 관계가 있는 것으로 추정된다. In order to investigate cAMP's involvement in the action mechanisms of gibberellic acid, the effects of cAMP on the biosynthesis and phosphorylation of proteins in the germinating maize seeds were studied. cAMP promoted the biosynthesis and phosphorylation of proteins. In the presence of actinomycin D, the stimulation of protein biosynthesis by cAMP was continuous during the germination period, while the protein phosphorylation was increased at the 3-day point after germination by cAMP. The stimulatory effect of cAMP on the protein biosynthesis was greater than that on the prtein phosphorylation during the early stages of germination. On the other hand, this pattern is reversed during the latter period. The above mentioned results are consistent with the facilitation effects of $GA_3$ on the protein biosynthesis and phosphorylation. Therefore, it seems to be that cAMP is related to the action mechanisms of $GA_3$.

Gibberellic Acid 의 작용기작에 관한 연구 ( 3 ) . cyclic AMP 에 의한 단백질의 생합성 및 인산화 반응의 조절

심웅섭,노광수,문혜연 ( Woong Seop Sim,Kwang Soo Roh,Hye Yeon Moon ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.1

In order to investigate cAMP`s involvement in the action mechanisms of gibberellic acid, the effects of cAMP on the biosynthesis and phosphorylation of proteins in the germinating maize seeds were studied. cAMP promoted the biosynthesis and phosphorylation of proteins. In the presence of actinomycin D, the stimulation of protein biosynthesis by cAMP was continuous during the germination period, while the protein phosphorylation was increased at the 3-day point after germination by CAMP. The stimulatory effect of cAMP on the protein biosynthesis was greater than that on the prntein phosphorylation during the early stages of germination. On the other hand, this pattern is reversed during the latter period. The above mentioned results are consistent with the facilitation effects of GA₃ on the protein biosynthesis and phosphorylation. Therefore, it seems to be that cAMP is related to the action mechanisms of GA₃.

한국산 aspergillus의 아밀라아제 활성에 관한 연구

심웅섭,윤경하,백락주,이영녹 한국미생물학회 1977 미생물학회지 Vol.15 No.1

Dextrinogenic and glucoamylase activities of Aspergillus isolated from various habitat-substrates collected through South Korea are measured, and their amylase activities are surveyed in taxonomical and ecological viewpoints. 1. A. flavous group and A.wentii group exhibited higher activities for both amylases than others. 2. In the relations between amylase activity of Asperguillus and their habitat-sub-strates, the strains isolated from meju and cereals exhibited predominant dextrinogenic amylase activity. 3. Dextrinogenic amylase activity of Aspergillus is higher in the strains isolated from southern coast than the other regions. 4. Among the 601 strains of Aspergillus surveryed, strain No.74 and strain No.421 exhibited the most predominant activity for dextrinogenic amylase and glucoamylase, respectively.

Ribosome 과 Elongation Factors EF - 1 과 EF - 2 의 활성에 미치는 cAMP의 효과

심웅섭,문혜연 생화학분자생물학회 1991 BMB Reports Vol.19 No.3

The effects of cAMP on the activities of the various components necessary for protein biosynthesis were investigated. 1. The rate of protein biosynthesis was increased about 27% by ribosomes treated with cAMP. 2. The activities of elongation factors EF-1 and EF-2 were considerably stimulated by exogenous cAMP.

Gibberellic Acid 의 작용기작에 (作用機作) 관한 연구 - 1. CA3 에 의한 단백질의 생합성 및 인산화반응의 조절

심웅섭(Woong Seop Sim),노광수(Kwang Soo Roh) 한국식물학회 1979 Journal of Plant Biology Vol.22 No.4

As a part of the studies on the regulatory mechanism of gene expression by GA_3, the effects of GA_3 on the protein biosynthesis and phosphorylation in maize seedlings were investigated. 1. The optimum concentration of GA_3 for the stimulation of the protein biosynthesis was 0.3mM. 2. The protein biosynthesis was remarkably increase by GA_3 during the germination. The reason for the decrease in the protein biosynthesis by 48hrs. after germination seems to be a staggered gene expression, and/or increases in protease and RNase activities. 3. The ratio of the amount of the newly synthesized protein in germination seeds treated with GA_3 to the amount of proteins secreted into the endosperm was similar to that ratio in control. According to this result, it seems that GA_3 stimulates only the expression of certain definite genes. 4. By the treatment with GA_3, the rates of biosynthesis and phosphorylation of proteins were increased up to about 1.5 times during germination and 6 times by 72hrs. after germination, respectively. The ratio of the total soluble proteins to the phosphoproteins considerably increased in teh early germination stage (24hrs.) but decreased after 24hrs. According to the above mentioned results, the stimulation of the phosphorylation of proteins by GA_3 seems to be attributed to the increases in the activities of protein kinases.

Gibberellic Acid 의 작용 기작에 관한 연구 - 2. Actinomycin D 처리시 GA3 에 의한 단백질의 생합성 및 인산화반응의 (燐酸化反應) 조절

심웅섭(Woong Seop Sim),박홍덕(Hong Duok Park),노광수(Kwang Soo Roh),문혜연(Hye Yeon Moon) 한국식물학회 1982 Journal of Plant Biology Vol.25 No.1

As a part of the studies on the regulatory mechanism of gene expression by gibberellic acid, the effects fo GA_3 on the protein biosynthesis and phosphorylation in maize seedlings were investigated in the presence of actinomycin D. The activites of protein biosynthesis and phosphorylation in germination seeds treated with GA_3 were greater than these of the control at the 3-day point after germination. It is assumed that the enhancement of protein biosynthesis by GA_3 in the presence of actinomycin D is due to the effects of GA_3 on the translational processes in which protein is produced from the mRNA synthesized previously.

Chlorella의 生長과 ChlorophyII 含量에 미치는 微量元素 缺乏의 影響

蔡麟基,沈雄燮 이화여자대학교 한국문화연구원 1967 韓國文化硏究院 論叢 Vol.11 No.-

In order to see if the growth of chlorella cells and chlorophyll content are influenced by the deficiency of microelements(Fe, Mo, Mn, Cn, and B), Chlorella ellipsoidea was inoculated on the normal medium (as a control) and each microelement-deficient medium and has been cultured for six days at 20-25℃ with 10,000 lux. As the results of the experiments, chlorella cells on microelement-deficient medium showed that packed cell volume cell volume and chlorophyll content were reduced in 6(Cu)~35%(Fe), 10(Cu)~50%, and 13(Zn)~43%(Fe), respectively, in comparison with the control, and the order of the influence of the deficiency is Fe>Mo, Mn, B, Zn>Cu.

참당귀(Angelica gigas Nakai) 뿌리의 면역증강활성 성분

안경섭,심웅섭,김환묵,한상배,김익환,Ahn, Kyung-Seop,Sim, Woong-Seop,Kim, Hwan-Mook,Han, Sang-Bae,Kim, Ik-Hwan 한국생약학회 1996 생약학회지 Vol.27 No.3

A potent immuno-stimulating activity was detected from the watersoluble and ethanol-precipitated crude extract (AG-1) of the root of Angelica gigas Nakai. The crude extract was fractionated into two fractions, an acidic AG-2 and a neutral AG-3 fraction by DEAE-cellulose adsorption. The two fractions contained polysaccharides of which M.W. were 10 Kdal and >2,000 Kdal respectively, proteins, and various inorganic components. The immunostimulating activities of two fractions were not reduced by proteinase K, acid or alkali treatment. The polysaccharides obtained from the root of A. gigas were mainly composed of arabinose, galactose, and galacturonic acid. These results indicated that immuno- stimulating components of the root of A. gigas was a kind of pectic polysaccharides or arabinogalactans.

Bradyrhizobium sp. SNU001의 nodD와 nodA의 염기서열

나영순,심웅섭,안정선 한국미생물학회 1993 미생물학회지 Vol.31 No.3

Nucleotide sequences of nodD and nodA from Bradyrhizobium sp. SNUOOI were determined. The open reading frame (ORF) of nodD was 942 bp in length and encoded 314 amino acids. while ORF of nodA, sequence of which is the first one among legume symbionts Bradyrhizobium, was 630 bp and encoded 210 amino acids. The nucleotide sequence of nodD showed 99.4% homology with nodDI of B. japonicum USDAllO. while that of nodA showed 81.5% with B. sp. (Parasponial. At the 5' of nodYAB operon and nodD, consensus nod box sequences composed of 9 bp unit repeated four times and two times respectively were found. Also an A.T-rich sequence was found at 5' of nodD. 대두(Glycine max) 뿌리혹의 질소고정 공생균주 Bradyhizobium sp. SNU001 의 nod D 와 nodA 의 염기서열을 결정하였다. 총 314개의 아미노산을 암호화하는 nod D 의 open reading frame (ORF) 은 942bp 로 B. japonicum USDA110 의 nodD1 과 99.4% 의 유사성을 보여주었으며, 총 210개의 아미노산을 암호화하고 콩과식물의 Bradyrhizobium 에서는 처음으로 염기서열이 결정된 nodA 의 ORF 는 630bp 로 B. sp. (Parasponia) 의 nodA 와 81.5% 의 유사성을 보여주었다. nodYAB 오페론과 nodD 상류에서는 9bp의 반복서열을 각각 4번, 2번 가지는 보존적인 nodbox 가 발견되었으며 nodD 의 상류에서는 A, T-rich 서열도 존재하였다.