纖維素 分解酵素 生産性 絲狀菌과 그 酵素活性에 關한 硏究

申炳湜,鄭圭永 건국대학교 1979 論文集 Vol.10 No.1

Total 60 strains were isolated from various sources such as soils, composte, and rottenwood, debris using cellulase screen media from the isolated 60 strains in the first experiments 8 strains were first selected text as good ones and identified, cellulase activities were tested The results of these studies are as follows; 1.Identified of 8 strains SK -17 ; SO-2, SW-1 ; Trichoderma viride ST-1 ; Penicillium sp. SO-9 ; Aspergillus oryzae SW-2 ; Aspergillus niger SO-5 ; Aspergillus ochraceus SBN ; Neuropora sp. 2.The strongest of celluase activity is SW-2, SBN and SO-9. The Mark SBN is the strongest in Carboxy Methyl Cellulose liquefaction and SO-9 is higher activities in cup method reaction. 3.In the test of the 1'eaction time of substrate and enzyme. The enzyme activities are high as prorata of reaction time of 24hr, CMC liquefaction become a half of total substrate. 4.According to cultural periods, enzyme activity is reahced the hight one in the 4 or 5 days cultural time airier the 5 days cultural time period enzyme activities was dwindled. 5.At the volume of CMC liquefaction is much, the index of refraction is high by method of diffrential refractometer. 6.In the teat of variation pH of substrate, good results come out at pH 4.6-5.0 of substrate. The optimum temperature was 45-50℃.

Vincristine Sulfate가 Mouse 모낭세포의 미세구종에 미치는 영향에 관한 전자현미경적 연구

신현태,이규식,류재만 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

Vincristine sulfate, one of thte Vinca alkaloids has been used clinically as an antitumor reagent. Vincristine sulfate has high affinity to the basic protein subunit of microtubules, that it disrupts the mitotic spindle apparatus. As a result this reagent causes the cell to arrest in metaphase and to die finally. When vincristine was spread on the skin it would damage the growth of the hair, that the author undertook the present study to observe the effect of vincristine onthe cytoplasmic organelles of the hair follicular cells. Male albino mouse, DDY strain, weighing 20gm were used as an experimental animals. The back skin of experimental animals were spread with a vincristine sulfate solution(1mg/5ml) once a day for 6 days after cutting of the hair. The experimental animals were sacrificed at 2 day, 4 day, and 6 day after first treatmetn and specimens obtained from the skin of the vincristine treated area. The specimens were prefixed in 2% glutaradehyde-2.5% paraformaldehyde solution and postfixed in 1% osmium tetroxide solution and then ultrathin section, 600-800A, were made and stained with uranyl acetate and lead citrate. Some specimens were also fixed in 10% formalin for hematoxylin eosin stain. The specimens were observed with light microscopy and JEM 100cx-II electrom microscopy. The results were as follows. 1. Hair follicles were decreased numerously in the skin of the 4th and 6th vincristine treated group. And the length of the hair was reduced in the skin of the 6th vincristine treated group. 2. In the 2nd vincristine treated group, irregularly shaped nuclei, reduced heterochromatin and numerous vacuoles wre observed in the external root sheath cells. In cells of the inner root sheath, heterochromatin was segregated and clumpped, and cristae of mitochondria was disappeared. 3. In the 4th vincristine treated group, dilatation of cisternae of the rough endoplasmic reticulum, degenerated mitochondria were observed in the external root sheath cells. And trichohyaline granules were reduced and condensed nuclei were observed in the cells of the Henley's layer. 4. In the 6th vincristine treated group, cuticle cells were more keratinized. And in the external root sheath cells, colummar shaped nuclei, degenerative mitochondria and rough endoplasmic reticulum, and decreased cisternae of Golgi apparatus were observed. Consequently, it is suggested that vincristine sulfute would induce the degenerative changes in the hair follicular cells.

피임제(Norinyl)가 흰쥐 난소의 Alkaline Phosphatase 활성에 미치는 영향

신경숙,이군자,정호삼,이규식 대한해부학회 1985 Anatomy & Cell Biology Vol.18 No.1

In mammals, female sexual hormones secrete at different rate during the sexual cycle so that the follicular cells and the granulosa cells undergo structural and functional changes. Therefore the authors undertook to pursue the changes of the graafian follicle during the estrous cycle and the effect of the oral-contraceptive. Female Sprague-Dawley rats weighing 150g were used as a experimental animal. Vaginal smears were obtained daily and only animals that showed consecutive four-day estrous cycle were used. The animals of the oral-contraceptive administered groups were administered norinyl, dissolved in distilled-water. The ovary was fixed with 10% neutral formalin and sliced at 14 ㎛ thickness in a frozen cryostat. The activity of alkaline phosphatase was observed by the Gomori's method for histochemical study. The other ovary was homogenized and the homogenate was assayed by the Lowry's method for biochemical study. The results were as follows. 1) In the follicle alkaline phosphatase activity was positive in the theca layer while the granulosa layer was negative. Strong activity were shown at proestrous and metestrous stage during the estrous cycle. And alkaline phosphatase activity of the ovary did not change markedly during the estrous cycle but the activity of estrous stage were slightly higher than other stages. 2) In the norinyl-administered group, the alkaline phosphatase activity of the ovary did not change during the estrous cycle. And the activity of the administered groups was lower than the control group.

위발성 위장관 악성림프종 환자에서 항암치료의 효과

김찬규,신영록,김현정,배상병,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Purpose: The gastrointestinal (GI) tract is the most common site of extranodal non-Hodgkin's lymphoma (NHL), which is increasing in incidence, but there is no established optimal treatment modality. Thus, this study was investigated the clinicohistologic feature, the therapeutic modalities, and the prognosis for GI-NHL, as well as the factors affecting it. Methods: We retrospectively analyzed 45 patients who had been diagnosed as having GI-NHL and had been followed up from July 1994 to February 2005 at Soonchunhyang University Hospital. The patients were divided into groups according to the site of origin and to various other features, and the survivals of the various groups were compared. The modified Ann Arbor system and WHO classification were adopted for staging and histopathologic classification, respectively. Results: GI-NHL of the stomach, small bowel, ileocecal region, and colon occurred in 28 patients (62.2%), 5 patients (11.1%), 3 patients (6.7%), and 8 patients (17.8%), respectively, In one patient, the entire gastrointestinal tract was diffusely involved. The median age of patients was b5 years (25~78 years), and male-to-female ratio was 1:1.1. Fourteen patients were in stage Ⅰ, 24 in stage Ⅱ, 4 in stage Ⅲ, and 3 in stage Ⅳ. Surgical resection was performed in 19 patients, and combination chemotherapy was performed in 43 patients. Surgical resection only was performed in 4 patients, Chemotherapy only was performed in 26 patients. The expected overall 5 year survival of 45 patients was 39.6%, and there was a significant survival difference between the stages, but between sites of origin (p=0.842). The most important factors influencing the survival was the stage and other factors were not significant. Conclusion: The stomach was the most common site of GI-NHL. Most GI-NHL were localized Stage was the most important prognostic factor. However, Prospective randomized studies are needed to approve the therapeutic modality.

Aspergilli에 대한 Penicillia의 Mycoparasitism에 관하여

鄭圭永,李馥權,申炳湜 건국대학교 1978 論文集 Vol.8 No.1

The parasitisms of one fungus on another has been recognized by mycologists for one hundred years. As early as 1865 and 1870, de Bary described and named Piptocephalis freseniana and Cicinnobolus cesati and recognized them as mycoparasites. In these experiments, the light and electron microscopic study of the host-parasite relationships of Penicillium rugulosum and Asperqillus spp was made to investigate pathological histology and anatomical structure and similarities and differences between the haustoria of mycoparasites and parasites of vascular plants. The hosts were identified to be Asperqillus flavus (A-1) and Aspergillus oryae (A-2) and the parasite to be Penicillum rugulosum. The parasite (Penicillum rugulosum) was isolated from the host by method of Berry & Barnett and inoculated and cultured on the Synthetic medium (Czapek'a Dox ager), so the parasite is non obligate. The haustoria of the mycoparasite, Penicillium rugulosum, on Aspergillis flavus are similar to those of parasitic fungi on vascular plants. An electron-dense sheath bounded by a membrane surrounds the haustroail wall. The sheath membrane appears to be the invaginated host ectoplast. No collar is formed around the haustorium by the host wall.

Interleukin-2가 Mouse 자궁내막의 Acid Phosphatase 활성에 미치는 영향

김원규,신규철,정호삼,이규식 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.2

Interleukin-2, a kind of lymphokine produced by the T-lymphocyte stimulated by antigen or mitogen, has been studied intensively with the development of immuno-oncology and chemoimmunotherapy of the malignancy. Interleukin-2 is known to exert on antitumor effect by stimulating the proliferation of helper and cytotoxic T lymphocytes, augmenting the cytolytic activities of the cytotoxic T lymphocyte and differentiating a precursor cell into a lymphokine-activated killer cell in vivo. Though interleukin-2 suppresses the metastasis of the malignant tumor into the adjacent organs, it develops many side effects including fever, chills, malaise, mental confusion, diarrhea, vomiting and oliguria. Interleukin-2 also causes pulmonary, hepatic and renal toxicities and, especially, increase of capillary permeability with weight gain, pulmonary edema and ascites. In this experiment, the author studied the effect of interleukin-2 on the mouse endometrium, observing histochemically the changes in the activity of acid phosphatase following the estrous cycle. Female, DDY strain, weighing 20gm and exhibiting normal estrous cycle, were used as experimental animals. The estrous cycle was recognized by the vaginal smear. The experimental animals were treated with 2 million units of interleukin-2/0.2ml per Kg of body weight and with 0.2ml of normal saline per Kg of body weight in the control groups for 5 days. And animals were sacrificed 5th day after administration of interleukin-2. The specimens obtained from the corpus uteri were fixed in 10% neutral buffered formalin solution for 24 hours at 4℃ and sliced at 14μm thickness in a frozen cryostat. The activity of acid phosphatase was observed by the Gomori's method for histochemical study. The result obtained was as follows: The acid phosphatase activity was increased at estrus and decreased at diestrus in interleukin-2 treated group. There was, however, no marked differences in acid phosphatase activity of the proestrus and metestrus between control and interleukin-2 treated groups. Consequently, it was suggested that interleukin-2 develop the changes of the acid phosphatase of the endometrium of the mouse.

악성림프종 환자에서 복합화학요법으로 유발된 백혈구 감소증에 대한 인형 재조합 과립구 집락형성-촉진인자(rhG-CSF: Neutrogin)의 효과

박성규,전진우,기신영,유병우,김홍수,백승호,원종호,홍대식,박희숙 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

Clinical effects of rhG-CSF were investigated in 17 patients receiving chemotherapy for non-Hodgkin's lymphoma. Patients were given G-CSF 2㎍/㎏/day after 2nd cycle chemotherapy for least 14 consecutive days, and clinical and laboratory results were compared to results of the first cycle. Treatment with G-CSF resulted in increase of mean WBC count(952.9±152.2㎜³vs 1882.4±350.4/㎜³) and neutrophil count(212.9±53.0/㎜³vs 841.5±238.0/㎜³), reduced the duration of leukocytopenic days(12.4±1.3 days vs 5.5±1.0 days, WBC count less than 4000/㎜³) and neutropenic days(4.5±0.9 days vs 2.3±0.7 days, neutrophil count less than 500/㎜³), and reduced the duration of neutropenic fever(5.8±1.1 days vs 0.9±0.5 days). The side effects of G-CSF were not so significant. These results demonstrated that G-CSF is safe and useful for the treatment of neutropenia induced by anticancer chemotherapy of non-Hodgkin's lymphoma and reducing the incidence of febrile periods and duration of antibiotic administration.

Cis-Dichlorodiammineplatinum이 Mouse 간장의 인산염 분해효소활성에 미치는 영향

유신철,이동준,정호삼,이규식 漢陽大學校環境科學硏究所 1987 環境科學論文集 Vol.8 No.-

cis-Dichlorodiammineplatium(Ⅱ)(cis-DDP)은 최근에 개발된 효과적인 종양화합 요법제인 금속화합물로서 그 작용기작은 아직 불명하나 bifunctional alkylating agent와 유사한 작용 기전을 갖는 것으로 알려져 있다. cis-DDP는 체내에서 가수분해되어 DNA와 반응함을 핵산의 합성을 억제하여 암세포 뿐만 아니라 정상세포에도 영향을 미쳐 출혈성장염, 임파조직과 골수의 저세포증, 신손상, 단백뇨 및 혈뇨등의 부작용을 유발한다. 이에 저자는 cis-DDP가 mouse 간장에서 세포독성으로 작용하여 간실질세포의 변성을 일으키리라 사료되어 간실질세포내의 acid phosphatase, alkaline phosphstase 및 aden-osine triphosphatase의 변동을 조직화학적으로 관찰하였다. 실험동물은 DDY계의 웅성(체중 20g)mouse를 사용하였으며 체중 kg당 6mg의 cis-DDP를 투여한 후, 6시간, 12시간, 24시간 및 48시간 경과후에 희생하여 간조직을 적출하고 10% formalin용액에 고정하고 cryostat로 16㎛두께로 동결절편을 만들었다. 조직화학적인 연구를 위하여 acid phosphatase와 alkaline phosphatase의 활성은 Gomori 방법으로 그리고 adenosine triphosphatase의 활성은 Wachstein-Meisel 방법으로 이들 효소활성을 관찰하여 다음과 같은 결과를 얻었다. 1. Acid phosphatase 활성은 간소엽주변대에서 cis-DDP투여 6시간 경과군의 mouse에서는 약한 양성반응을 12시간 경과군의 mouse에서는 중등도의 양성반응을 48시간 경과군의 mouse에서는 약한 양성반응을 나타내었다. 2. Alkaline phosphatase 활성은 간소엽 주변대에서 cis-DDP투여 12시간, 24시간, 및 48시간 경과군의 mouse에서 중등도의 양성반응을 나타내었다. 3. Adenosine erihosphatase 활성은 간소엽 중심대에서 cis-DDP투여 6시간 경과군의 mouse에서 미약한 양성반응을 나타내었고, 12시간 경과군의 mouse에서는 중등도의 양성반응을 나타내었고, 24시간 및 48시간 경과군의 mouse에서는 각각 중등도 및 강한 양성반응을 나타내었다. 이상의 소견을 종합한바 cis-DDP의 투여로 mouse의 간장 손상을 일으켜 acid phosphstase, alkaline phospatase 및 adenosine triphosphatase 활성은 일시적으로 저하되었다가 회복되는 것으로 사료된다. cis-Dichlorodiammineplatinnm(Ⅱ) (cis-DDP), known as cisplatin, is a metallic compound that has recently been developed as an effective cancer chemotherapeutic agent. The precise mechanism of action of cis-DDP is still unknown. Similarities between the mechanism of action of this drug and bifunctional alkylating agents have been suggested. Upon entering the cell, the platinum compounds will become hydrolyzed, and then they can react with DNA. They act on tumor cells as well as normal cells. Side effects included severe hemorrhagic enterocolitis, severe hypocellularity of the bone marrow and lymphoid tissues, renal lesions, proteinuria and (microscopic henaturia). In this experiment, the author studied the effect of cis-DDP on the mouse liver, histochemically observing the changes in the activities of acid phosphatase, alkaline phosphatase, and adenosine triphosphatase. Male mice (the weight 20g), DDY strain, were used as experimental animals. The experimental animals were sacrificed at 6,12,24 and 48 hours after administration of 6mg of cis-DDP per kilogram of body weight. The specimens obtained from the liver were fixed in 10% formalin solution and sliced at 16㎛ thickness in a frozen cryostat. The activities of acid and alkaline phosphatases were observed by the Gomori's method and the activity of adenosine triphosphatase was observed by the Wachstein Meisel's method for histochemical study. The results are as follows. 1. The acid phosphatase activity was weakly positive at 6 hours and moderately positive at 12 hours and weakly positive at 48 hours after administration in the peripheral zone of the hepatic lobule. 2. The alkaline phosphatase activity was decreased to moderately positive at 12,24 and 48 hours after administration in the peripheral zone of the hepatic lobule. 3. The adenosine triphosphatase activity was trace positive at 6 hours after administration in the central zone of the hepatic lobule and moderately positive at 12 hours after admnistration in the central and peripheral zone of the hepatic lobule. It is suggested that cis-DDP causes changes in activities of acid phosphatase, alkaline phosphatase and adenosine triphophsatase in the liver, due probably to the cytotoxic effect in the parenchymal cells of the liver in mice.

Mitomycin이 Mouse 간장의 인산염분해 효소에 미치는 영향

정호삼,신규석,이군자,이규식 한양대학교 의과대학 1986 한양의대 학술지 Vol.6 No.1

Mitomycin (MC), a wide spectrum antibiotic and anticancer drug, is isolated from streptomyces caespitosus. MC decomposes DNA in cancer cell by forming a complex with DNA and inhibits division of cancer cells by interfering with the biosynthesis of DNA. In the normal cell, MC cross linkes with DNA and inhibits synthesis of RNA and protein. Therefore MC causes many complication in the MC treated patient. The authers have investigated the effect of MC on the mouse liver histochemically observing the change in the activities of alkaline phosphatase and ATPaes. Male mouse, DDY strain, weighing about 20 grams was used as experimental animals. The experimental animals were divided into the control and MC treated group and given food and water unlimited. The animals treated with 4mg of MC per kilogram of body weight were sacrificed at 6, 12, 24, 36 and 48 hour after administration. The animals of control groups were administered only water for injection. The liver specimens were fixed in 10% formalin solution at 4℃ and sliced at 14㎛ thickness in a frozen cryostat. The activities of alkaline phosphatase were observed by the Gormori's method and those of ATPase were observed by the Wachstein-Meisel's method for histochemical study. The result are as follows. 1. As the time passed, the activity of alkaline phosphatase activity was decreased in the hepatic lobule. Negative reactions were showed in the central and intermediate zone of the lobule of 36 hours of MC treated group. Trace activities of the enzyme were observed in the whole hepatic lobule of 48 hours of MC treated group. 2. As the time goes by, the activity of adenosine triphosphatase was decreased in the hepatic lobule. Negative reactions were observed in the entire hepatic lobule of 36 hours of MC treated group. Weak activity of the enzyme were observed in the whole hepatic lobule of 48 hours of MC treated group. Consequently, it is suggested that MC causes decrease in activities of hepatic alkaline phosphatase and ATPase due to live damage by cross linking of DNA with the drug in the hepatocyte and destructive change of organelles of the cell.

양측성 알도스테론 분비 선종 1 예

김동규,김규태,이상조,신형식,김학양,유재영,배수동,임성희,김병태,김종혁,송숙희 대한내과학회 1991 대한내과학회지 Vol.41 No.5

Primary aldosteronism is a condition in which chronic aldosterone excess exists independently or semiindependently of the renin-angiotensin system, resulting in hypertension and hypokalemia. The most common cause of primary aldosteronism is unilateral aldosterone-producing adrenal cortical adenoma. The indiopathic aldosteronism showing bilateral adrenocortical hyperplasia accounts for the bulk of the remaining cases. Only rare cases of primary aldosteronism are known to be due to multiple aldosterone-producing adrenocortical adenoma. We report a case of primary aldosteronism due to bilateral adenomas, which was diagnosed preoperatively by abdomen CT and then confirmed by operation and pathological findings.