Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

가와사끼 병 환아에서 이상뇌파와 신경학적 합병증 발현과의 연관성에 대한 고찰

박준수,공도연,박경배,민용식,김영창,정순이,이혜경 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

목적 : 가와사끼 병의 뇌파 소견 및 다른 임상인자들과의 연관성을 알아보고자 하였다. 방법 : 1994년 1월부터 1995년 12월 사이에 순천향대학 천안병원 소아과에 입원한 23명의 신경학적으로 정상인 가와사끼 병 환아에게 뇌파검사를 실시하여 이를 검사시기, 성별, 연령, 혈소판, 간기능 검사치, 그리고 동맥류 유무등의 임상인자들과 비교하였다. 결과 : 이상뇌파가 23명중 52.2%인 12명에게서 관찰되었으며, 이상뇌파로는 예파 및 극파, 극·서파 복합 양상과 전반적인 배경파의 서파양상이 나타났다. 뇌파의 검사시기가 진단부터 1주 이내인 경우에 이상뇌파의 빈도가 그 후보다 의미있게 높았으나, 그 밖의 다른 임상인자와의 연관성은 별 의미가 없었다. 결론 : 가와사끼 병 초기에 이상뇌파가 나타나는 것은 비록 신경적 증상은 없지만 중추 신경계가 영향을 받는다는 간접적인 증거로 생각할 수 있어 향후 신경적 합병증 발생 여부에 대한 면밀한 관찰이 필요하다고 생각된다. Purpose : This study is to find out if there is any association between EEG abnormalities in Kawasaki disease with other clinical factors. Methods : We performed EEG in 23 Kawasaki disease patients without any neurologic sign who admitted in Soonchunhyang Chunan Hospital from 1994. 1. to 1995. 12. and compared it with sex, age, platelet count, liver function test and presence of coronary artery aneurysm. Results : Abnormal EEG findings were observed in 12 of 23 patients(52.2%) and as for abnormal EEG findings, sharp, spike, spike & slow wave complex, and generalized slow background activity were noted. When EEG was performed within a week after the diagnosis, the incidence of abnormalities of EEG increased significantly but there was no correlation with other clinical factors. Conclusion : EEG abnormalities in the early stage of Kawasaki disease indicate that although there is no abnormal neurologic signs clinically, central nervous system might be affected and there should be a close observation for the developement of neurologic complication.

日本腦炎 바이러스에 대한 動物의 血淸學的 硏究

朴京洙,朴悅 朝鮮大學校 附設 醫學硏究所 1984 The Medical Journal of Chosun University Vol.9 No.1

Japanese Encephalitis Virus liaa been widely known aa prime acute cummunicablc viral disease during the summer season in Korea. Many basic problems such as virus hibeniating, vector resting habit and hibernation were still remained to be solved although so many researches of this field had been done. And then, the incidences of Japanese Encephalitis Virus were so various that predictions of the epidemic size were not always coincident. The purpose of this report was to present the seasonal occurrence of Japanese Encephalitis Virus from the slaughter house swines in Chunnam from June 28, 1982. The study was also carried out with the epidemiological relationship of Japanese Encephalitis Virus antibody distribution in fowls, ducks, and cats at Chunnam area during September. 1982. The present investigation was preformsd by using hemagglutination inhibition test (Aimproved method of National Institute of Health Republic of Korea, 1978.) The obtained results were as follows: 1). Results of HI antibody positive rate in swines of slaughter house from June 28, 1982 to September 1, 1982 at Chunnam area showed as follows; They represented 10% on July 20, 18% on August 9, 75% on August 16, 94% on August 24, and 100% on September 1. 2). HI antibody positive rates of provincial distributions in the fourteen fowl areas were different from each other and they were 27 cases (13.7%) among 197 cases. 3). HI antibody positive rates of provincial distributions in the ten duck areas were different from each other and they were the highest as 67 cases (64.5%) among 107 cases. 4). HI antibody positive rates in the cat were 2 cases (15.4%) among 13 cases. 5). Japanese Encephalitis Virus antibody rates in geographical distribution of the fowl and the duck showed more higher in the coastal areas than in the inland areas.

면역 부전 환자에서 병발한 급성 황색 포도상 구균에 의한 세균성 식도염 1예

박환양,김춘관,박영배,박재완,김성훈,강정원,문경협,양수현,변종훈 대한감염학회 2001 감염 Vol.33 No.5

Bacterial esophagitis is an uncommon disease and has not been well characterized. Bacterial infection of the esophagus is usually presented as a superimposed infection upon a preexisting viral or fungal esophagitis and most patients are immunocompromised hosts. A 67-year-old man was admitted for retrosternal pain and hematemesis, who had a past history of long-standing diabetes mellitus and end stage renal disease, also had a history of steroid medication. Extensive esophageal ulcerations of the mucosa were visualized by endoscopy. Staphylococcus aureus grew in blood culture. After the 2 weeks of antibiotics treatment, he was successfully recovered without any sequelae. Due to its rarity, this case is herein reported with a review of the corresponding literature. (Korean J Infect Dis 33:360∼363, 2001)

MMPI를 이용한 악교정 수술환자의 인성에 관한 연구

박우경,박재억,박수병 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.5

이 연구에서는 악교정 수술환자의 술전, 술후 인서의 특성 및 술후 변화를 알아보기 위해 실시되었으며 객관적인 인성검사의 한가지인 MMPI를 이용하였다. 악교정 수술환자들의 수술동기와 술후 만족도는 질문지를 통하여 조사하였다. 연구대상은 술전교정 중인 환자 50명과 악교정 수술을 받은 환자 22명으로 이루어졌다. 이 연구에서 얻은 결과는 다음과 같다. 1. 술전 환자군에서 K 척도를 제외한 모든 T-scores는 정상적인 범주에 놓여 있었다. K 척도값은 57.7이었으며 이것은 이 환자들이 다소 스트레스를 많이 받고 있음을 보여주었다. 2. 술후 환자군은 모든 척도에서 정상범주에 놓여 있었다. 3. 술전, 술후 환자군을 비교했을 때, 단지, T-score만이 수술 후에 통계학적으로 유의성있는 증가를 보였다.(P<0.05) 4. 남성환자는 술전 K 척도를 제외한 모든 척도에서 정상적이었으며, 술후에 K, Hs 척도에서 통계학적으로 유의성있는 증가를 보였다.(P<0.05) 5. 여성환자는 모든 T-score기 정상적인 범주에 놓여 있었다. 6. 수술동기는 크게 심미적, 기능적, 악관절문제, 정신사회학적 동기로 나눌 수 있었으며, 각각 28.2, 32.1, 9.0, 30.7%였다. 7. 수술 후 수술 동기에 대한 만족도는 매우 만족하는 환자가 전체의 14.3%, 만족하는 환자가 71.4%, 그저그렇다고 답한 환자가 9.5%였으며, 매우 불만족스러움을 답한 환자가 4.8%였다. This study was designed to evaluated the personality characteristics of orthognathic surgery patients preoperatively and postoperatively through the MMPI. Comparison was made between the personality characteristics of preoperative and postoperative groups. Motivation for undergoing orthognatic surgery and postoperative satisfaction were examined trough the questionnaire. Subjects used in this study consisted 50 patients under presurgical orthodontic treatment and 22 patients who had received orthognathic surgery. From this study, the results were followings. 1. In preoperative patients group, all T-scores were within the normal range except for K-scale. The T-score of K-scale is 57.7, this showed that these patients underwent some of less stress. 2. In postoperative patients group, all T-scores were within the normal range. 3. Only in K-scale, there was statistically significant increase after surgery(P<0.05). 4. In male patients group, all T-scores were within the normal range except for that of preoperative K-scale. Postoperatively, there were statistically significant increase in K and Hs scales(P<0.05). 5. In female patients group, all T-scores were within the normal range. 6. Motivation for undergoing orthognathic surgery could be divided into 4 categories.:esthetic goal(28.2%), functional goal(32.1%), TMJ problem(9.0%), and psychosocial reason(30.7%). 7. The degree of postoperative satisfaction was quite high(85.7%). 14.3% of all patients were very satisfied, 71.4% were satisfied, 9.5% were neither satisfied or dissatisfied, and 4.8% were very dissatisfied.

Relevance feedback을 이용한 감성언어 기반 영상 검색

박중수,엄경배 국립7개대학공동논문집간행위원회 2002 공업기술연구 Vol.2 No.-

We propose an on-line query mechanism for emotion-based similarity retrieval of images in database. It uses emotional languages for the first search. We know that it successfully catches the user's emotion. For the second search, we use relevance feedback algorithm to reflect the gap between the user's emotion and computer calculated contents. Relevance feedback successfully analyzes the data that is user's estimation of images and reduces the gap between user and computer. The features and similarity measures are adopted from MPEG-7 color descriptors which are proper retrieval of large multimedia databases. We use 286 images for experiment which are corrected from internet. The result shows that the system is successful.

Boosting algorithm을 이용한 다중 질의 영상검색

박중수,엄경배 국립7개대학공동논문집간행위원회 2002 공업기술연구 Vol.2 No.-

Content-based image retrieval uses extracted image contents. But if the contents are consist of many features then the retrieval process may speed down. In this paper, we use boosting algorithm to reduce the redundancy of features. We experiment 286 wallpapers and get the successful result.

안정한 지지면과 불안정한 지지면에서 팔굽혀펴기 운동시 견갑골 주위 근육의 근활성도 비교

박수경,이현옥,김종순,김선엽 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

The couple force of the scapular stabilizers for upward rotation that include the upper and lower trapezius, serratus anterior muscles is essential to maintain the stability of shoulder joint. But if there is and imbalance of these muscles, it would occur many problems of shoulder joint. A push-up plus exercise with an unstable support is used in order to increase the muscle activity and stimulate the proprioception of shoulder joint. The purpose of this study was to compare the muscle activity of upper and lower trapezius, serratus anterior muscles and to determine which exercise is more effective when subjects perform push-up plus exercise in the stable support and unstable support. 15 healthy subjects perform push-up plus exercise in the stable support and unstable support. 15 healthy subjects and 15 painful subjects with injury of shoulder joint participated in this study. Surface electromyography data were collected during plus phase of push-up plus exercise. The types of push-up plus exercise were composed of three conditions. Stable type is subjects performed the push-up plus exercise on the fixed support and unstable type 1 is on the sling without shaking and unstable type 2 is on the sling with shaking by tester's manual. The upper and lower trapezius activities of injured group were higher than uninjured group at three measure conditions, but serratus anterior activities were not. The UT/SA ratio of injured group was higher than uninjured group at three measure conditions. The serratus anterior activities were most high at unstable 2 measure in both groups. The UT/SA ratio was most low at unstable 2 measure condition in injured group only. The present result revealed that push-up plus exercise in the unstable support with shaking which took most high serratus anterior activities and most low of UT/SA ratio is optimal cure method that can improve the imbalance of the scapular stabilizer.

Newcastle병 면역에 대한 연구

박경수,김종세,노영복 조선대학교 기초과학연구소 1987 自然科學硏究 Vol.10 No.1

A study was conducted to determine whether the vaccination programs for the control of Newcastle Disease (ND) would affect the immune status of hens against the disease as a purpose of suggesting the distinguished vaccination programs. Fifteen poultry flocks were surveyed to investigate the level of serum antibody against ND virus and the programs used for the vaccination of hens. Alternatively, vaccination was carried out by the programs designed against ND virus in hens called Manina and investigated the level of HI titer every week, and surveyed the alternation of hematological occurrence following the vaccination programs. The obtained results were summarized as follows: 1. Vaccination history of the investigated 15 poultry flocks were not according by standard vaccination programs and immunization was carried out for random at every poultry farms. Killed and Live vaccin was used together in 7 groups and only Live vaccin was used in 6 groups, and only killed vaccin was used in groups. 2. HI titer of these was 140 in average by killed and live vaccin, and live vaccin was 126 in average. The former was shown higher HI titer. 3. The number of leucocytes was shown much increased hy the designed vaccination program, and 9525/㎣ was shown by 2nd vaccination (9weeks), and 1067O/㎣ was shown highest by 6th vaccination (25weeks). 4. HI titer was 240 by 2nd vaccination and it was continuous to increase. HI titer was 320 by 6th vaccination. The effects of the repeated vaccination were distinguished. 5. Serum globulin value was increased according to the vaccination program. The highest value ware 3.54㎎/㎗ and 3.54㎎/㎗ in 22 weeks and 23 weeks, each γ globulin value of these were 0.95㎎/㎗ and 0.99㎎/㎗.

백서의 치아이동 시 피질골 천공이 치주조직의 OPG, RANK, RANKL의 발현에 미치는 영향

박우경,김성식,박수병,손우성,김용덕,전은숙,박미화 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.3

치아이동 시 피질골 천공이 치조골 재형성에 미치는 영향을 알아보기 위해서 생후 15주된 자성백서를 사용하여 피질골 천공 후 치아이동을 실시한 실험군(Tooth movement with cortical punching: TMC group, n = 16)과 교정적 치아이동만 실시한 대조군(Tooth movement only group: TM group, n = 16)의 치아주위조직을 면역조직화학염색을 통하여 관찰하였다. 실험군과 대조군의 실험동물에 20 gm의 힘으로 상악 전치부 사이를 이개시키는 치아이동을 시행하였으며 실험군에서는 상악 전치부 구개부위에 피질골 천공을 실시하였다. 치아이동 후 1, 4, 7, 14일째에 실험군과 대조군의 실험동물을 희생시켰다. 면역조직화학염색법으로 OPG, RANK, RANKL의 발현을 비교한 결과, OPG의 발현은 양 군 모두에서 미처치 대조군에 비하여 감소되었으나, 실험군에서의 발현이 대조군보다 컸으며, RANK, RANKL은 피질골 천공을 시행한 경우에 더 강한 발현을 보이는 것이 관찰되었다. 따라서 피질골 천공이 치주조직의 OPG, RANK, RANKL의 발현에 영향을 미치며 치조골의 재형성을 향상시키는 것을 알 수 있었다. Objective: The purpose of this study was to investigate whether cortical punching could stimulate the expression of OPG, RANK, and RANKL during tooth movement by immunohistochemistry. Methods: 34 sprague-dawley rats (15 weeks old) were allocated into 3 groups: TMC group (experimental group; Tooth Movement with Corticotomy, n = 16), TM group (control group; Tooth Movement only group, n = 16), and non-treatment group (n = 2). 20 gm of orthodontic force was applied to rat incisors by inserting elastic bands. The duration of force application was 1, 4, 7 and 14 days. A microscrew (diameter 1.2 mm) was used for cortical punching of the palatal side of the upper incisors in the TMC group. Results: Distributions of OPG, RANK, and RANKL were evaluated by immunohistochemistry. OPG, RANK and RANKL were observed on experimental and control groups. On the compression side, the degree of the expression of OPG decreased in both groups. The expression of RANK was most prominent in the experimental group of day 4. The expression of RANKL was most intensive and extensive in the experimental group of day 7. However, the expression of OPG was decreased in the experimental and control groups compared to the non treatment group. The expression of OPG, RANK and RANKL after force application were decreased at day 14. Conclusions: These findings suggested that cortical punching might stimulate remodeling of alveolar bone during a 2 week period of tooth movement without any pathologic change.