Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

첨가성분에 따른 부가중합 실리콘 인상재의 세포독성에 관한 연구

조리라,고성희,정경호,김경남 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.3

The purpose of this study was to evaluate the biocompatibility of polyvinyl siloxane impression materials and it's components using cell culture techniques. In the cytotoxicity test, using the culture of L929 mouse fibroblast, the degree of gross cytotoxic effects on the cells by changes in the cell morphology was scored for each materials. And the changes in the cell membrane permeability was tested using the agar overlay test. The results were as follows: 1.The effect of Examix impression material had on cell viability by evaluating survival rates was more severe than any other materials and newly added surfactants. 2.NP-4, nonylphenoxy polyethylene ethanol homologs, showed some cytotoxicity than PC-550 and BYK066. 3.In the test of the changes in cell membrane permeability using the agar overlay method, any cytotoxicity was recorded in silicone base groups. 3.In agar overlay test, Examix and NP-4 showed most response index and lysis index. 4.The application of surfactant such as PC-550, NP-4, and BYK066 might be considered relatively biocompatible.

악성 혈액질환에서 성공적인 동종골수이식 후 숙주 기질 미세환경의 구축

조상희,이제중,남찬은,최경상,정익주,이일권,김진희,박종태,김형준 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1

인체의 골수는 간엽모세포를 함유하고 있으며 이들은 골수미세환경의 주된 세포들로 분화가 가능하여 조혈기능을 지지한다. 본 연구에서는 성별이 다른 동종조혈모세포이식 환경에서 조혈모세포의 완전 생착을 보이고, 이식 후 1년에서 8년이 지난 11예의 재생불량성 빈혈 및 백혈병 환자들을 대상으로 하여 골수에서 MSC를 분리하고 체외 확장을 통해 배양된 MSC에서 X 염색체 탐식자를 이용한 FISH 및 microsatellite polymorphism PCR 기법으로 그 기원을 확인하였다. 그 결과 조혈모세포는 완전히 공여자 기원으로 대치되었음에도 불구하고 MSC는 모두 수여자 기원임을 알 수 있어, 동종조혈모세포이식에서 미세환경의 구축은 수여자의 자가 생산에 의한 골수 간질세포에 의한 것으로 생각된다. Background: Human bone marrow (BM) contains mesenchymal stem cells (MSC) that can differentiate into various cells of mesenchymal origin. It remains a matter of controversy whether donor-derived stromal cells are capable of engraftment following hematopoietic stem cell transplantation (HSCT) or not. Methods: To determine if donor-derived stromal cells are transferred to the recipients of allogeneic HSCT, we investigated the characterization of MSC in 11 patients 1 to 8 years after sex mis-matched allogeneic HSCT in severe aplastic anemia and leukemia. Results: All patients had complete engraftment with donor- derived stem cells as shown by detection of donor type DNA in peripheral blood mononuclear cells. Following culture, MSC showed the expression of SH2 and SH4, but none of the hematopoietic markers of CD14, CD34, or CD45. MSC which can be differentiated to osteogenic lineage showed the genotype of recipient completely using FISH or PCR analysis. Conclusion: This study confirmed that MSC isolated from recipients of allogeneic HSCT in severe aplastic anemia and leukemia are not of donor genotype despite of full hematopoietic engraftment with donor type. Donor cells did not contribute to reconstitute the marrow microenvironment.

유산균을 이용한 경구용 항원 단백질 수송능 연구

조희정,최한곤,김정애,오유경 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.2

A promising application of Lactococcus lactis is its use as live vehicles for production and delivery of heterologous proteins of vaccines and therapeutic substances. Because L. lactis has GRAS ("generally regarded as safe") status, use tested whether L. lactis could function as the carrier of the L1 protein of human papillomavirus (HPV) type 16. The RNA level expression of L1 gene was detected in L. Laois. The L1 protein was expressed in L. luctis with LI gene. The growth of strains L. lactis with an empty plasmid (pAMJ328) and L. lactis with L1-encoding plasmid (pAMJ328-L1) was slightly decreased in comparison with the growth of strains L. lactis (wild type). However, all the three strains of L lactis maintained the ability to ferment sugars primarily into lactic acid, indicating that L1 protein did not affect the biochemical property of L.. lactis. These results suggest that L. lactis. capable of carrying LI protein, might he further developed as a biocompatible oral protein delivery system.

폐탄광 배수에 의한 주변 화천 오염 실태 조사

조경숙,장용근,류희욱 崇實大學校 生産技術硏究所 1996 論文集 Vol.26 No.1

The survey was carried out to investigated the contamination of streams by the acid mine drainage originates from abandoned coal mines and coal refuse piles. The physico-chemical characteristics such as pH, sulfate and elements concentrations of waters and sediments in streams were analyzed, and the microbial activities in the sediments were evaluated by measuring dehydrogenase activities. In sites contaminated by the acid mine dranage, the pH of waters adn sediments declined to acid from neutral due to the accumulation of sulfate. Dehyfrogenase activities ranged from 12 to 170 ㎍-TPF·g-dry soil-¹·24h-¹in the contaminated sites, whereas teh uncontaminated sites had activities of 1,176-4,259 ㎍-TPF·g-dry soil-¹·24h-¹. Dehydrogenase activities were significantly affected by low pH of sediments, indicating that high strength of sulfate inhibited microbial activities. The concentrations of heavy matals such as Pb and Fe in contaminated sediment (37-46 ppm Pb; 46,000 ppm Fe) were much higher than those in the uncontamnated sediments. concentration of Al in the contaminated water acidfied by the abandoned coal drainage was in range of 11 to 42 ppm. compared with those in the uncontaminated sediments, the concentrations of Mn, Mg and Ca in contaminated sediments were low because of the leaching of them from soil to water by acidfied stream water.

文學硏究會 小考

趙璟姬 中國語文硏究會 1992 中國語文論叢 Vol.5 No.-

일개 간호학과 학생을 대상으로 자기효능감과 대처정도의 상관성 분석

조남옥,엄영란,유경희 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

The purpose of this study was to analysis the correlation between self-efficacy and level of coping in nursing students. Subjects of the study were 134 nursing students. Self report questionnaires were used to measure the variables including self-efficacy and level of coping. Reliability coefficients of the self-efficacy and coping instruments were found Cronbach's α=.80 ~.91. In data analysis, SPSS PC 6.0 Program was utilized for descriptive statistics, Pearson's correlation, One-Way Anova. The results of Pearson's correlation and One-way Anova were as follows. 1) The self-efficacy was significant correlation with level of coping(r=.246, p<.005) and the number of year in campus life(r=.506, p<.0001). 2) There was significant difference in self-efficacy according to the number of year in campus life(F=16.52, P<.0001). 3) There was significant differences in the level of coping according to the satisfaction of campus life(F=3.02, P<.05) and self-efficacy(F=4.2621, P<.05). Based on the results obtained from this study, strategies to ptomote self-efficacy have to be developed in nursing curriculum.

부분 통합교육을 받는 정신지체아동과 일반아동간의 상호인식 연구

조경희 대진대학교 교육연구소 2000 교육연구 Vol.2 No.1

In order to facilitate the integration of students with mental retardation with their nonhandicapped peers, it is important to examine the attitudes and perceptions of both groups of children. This research project examined the perception that students with mental retardation and their nonhandicapped peers had about each other at the conclusion of integration. This qualitative reaearch study utilized individual interviews to gather data from the students with mental reatardation and their nonhandicapped peers.

魯迅 前期文學에 나타난 女性觀

趙璟姬 中國語文硏究會 1995 中國語文論叢 Vol.9 No.-

부산․경남 지역 광고 관련 학과 교과과정에 나타나는 스피치 과목에 관한 연구

조경섭 ; 김은희 동의대학교 인문사회연구소 2009 인간과 문화 연구 Vol.- No.15

In Advertising․PR field, presentation skill is important in order to persuade advertisers, therefore speech education is very important for effective-development of the communication skills. Accordingly, this research analyzes the Advertising․PR relevant majors' curriculums and status of speech education within 13 Advertising․PR relevant universities at Pusan․Kyungnam territory, then examine the controversial points and future subjects. Result of this research appears that relevant subjects of 'executive ability' and 'creative ability' are insufficient, especially exceedingly insufficient about speech relevant subjects such as presentation and etc. 광고홍보학 분야에서는 광고주 설득을 위한 프레젠테이션 기술이 중요하므로 효과적인 커뮤니케이션 능력 배양을 위해 스피치 교육이 중요하다. 따라서 본 연구는 부산․경남 지역 광고홍보 관련 학과 13개 대학을 대상으로 광고홍보 관련 학과의 교과과정 및 스피치 교육 현황을 분석하고, 문제점과 향후 과제에 대해 점검해 보았다. 연구 결과, 광고 전문가들이 가장 중요하게 평가한 ‘실무능력’과 ‘크리에이티브 능력’과 관련이 있는 과목이 부족한 것으로 나타났으며, 특히 프레젠테이션 등의 스피치 관련 과목들이 매우 부족한 것으로 나타났다.