Immuno-modulatory activity of hot water extracts isolated from Protaetia brevitarsis seulensis larvae following treatment with Bacillus velezensis TJS119

Kook-Il Han,Jeong Tae Kim,Young Ho Nam,Jum Oc Jung,Eunsun Kim,Mi-Hwa Lee 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10

Protaetia brevitarsis seulensis larvae from industrial insects are traditionally recognized as functional health foods in South Korea. We evaluated the immuno-modulatory effects of feeding beneficial microorganism (Bacillus velezensis TJS119) to P. brevitarsis larvae as a dietary source. In this study, we investigated the immune-enhancing activities of P. brevitarsis larvae hot-water extract (PLW) and PLW after treatment with B. velezensis TJS119 (PLWB) using the RAW 264.7 macrophage cell line. We examined the effects of PLWB on cell proliferation, cytokine production, and nitric oxide production in RAW264.7 cells. PLWB showed no cytotoxicity at concentrations ranging from 7.8 to 1,000 μg/mL in RAW264.7 cells. Treatment with PLWB increased the production of nitric oxide and pro-inflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β)] at doses of 62.5 to 1,000 μ g/mL in RAW264.7 cells. As a result, PLWB exhibited a stronger immune-enhancing effect compared to PLW. In conclusion, the results of this study offer experimental evidence to support the potential utilization of PLWB as an immunity-enhancing nutraceutical ingredient.

Streptococcus dysgalactiae로부터 분리된 히알루론산과 황화된 유도체의 구조와 항염증 활성

홍창일(Chang-Il Hong),정의길(Eui-Gil Jung),한국일(Kook-Il Han),김용현(Yong Hyun Kim),이성희(Sung Hee Lee),이홍섭(Hong Sub Lee),한만덕(Man-Deuk Han) 한국생명과학회 2016 생명과학회지 Vol.26 No.5

히알루론산(HA, Hyaluronic acid)은 β-1, 3-N-acetyl glucosamine과 β-1, 4-glucuronic acid가 반복된 선형 폴리머 고분자로서 생물학적 활성 및 생체친화성 특성 때문에 의약 및 약학분야에서 중요한 분자로 여겨지고 있다. 본 연구는 HA을 S. dysgalactiae으로 얻고, 화학적 방법을 통해 황화된 히알루론산(S-HA, Sulfated hyaluronic acid)유도체를 합성하여 그 구조와 항염증 활성을 비교하였다. HA의 생산은 S. dysgalactiae를 5 l 생물반응기를 이용하여 대량 배양하여 수용성 히알루론산(HA-WS, water soluble hyaluronic acid)과 비수용성 히알루론산(HA-WI, water insoluble hyaluronic acid)을 분리 정제하였다. 특히 HA-WI를 황화시켜 황화된 히알루론산(S-HA) 유도체를 합성하였으며, 그 수율은 90%로 나타났다. 합성된 S-HA의 구조를 FT-IR 및 ¹H/<SUP>13</SUP>C-NMR를 통해 S. dysgalactiae 로부터 생산된 표준 HA, HA-WS 및 HA-WI와 비교 분석한 결과, 황으로 치환된 양상을 확인하였다. 또한, S-HA의 항염증 활성을 RAW 264.7 대식세포를 통해 확인한 결과, S-HA는 천연 형태의 HA (HA, HA-WS)보다 nitric oxide (NO)와 COX-2 및 PGE₂ 유전자 발현이 유의하게 낮게 발현되었다. 염증 매개 cytokine인 TNF-α (<80 pg/ml) 및 IL-6 (<100 pg/ml)의 생성도 S-HA가 천연 HA보다 낮은 수준으로 정량되었다. 이 같은 결과에서 황화된 S-HA은 천연 히알루론산보다 용해성이 우수하고 염증관련 사이토카인의 생성 억제를 통해 항염증 효과를 나타내므로 염증치료제, 성형 및 생체 적용 약물전달 소재로 그 활용이 기대된다. Hyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. HA is a natural and linear polymer composed of repeating disaccharide units of β-1, 3-N-acetyl glucosamine and β-1, 4-glucuronic acid. This work aimed to confirm the structural characteristics and anti-inflammatory activities of HA and its chemically sulfated-HA. HA was produced from a fed-batch fermentation process using Streptococcus dysgalactiae in a 5 l bioreactor. HA was isolated water-soluble form (HA-WS) and water-insoluble form (HA-WI) from culture medium, and was obtained chemically sulfated-derivative (S-HA) that resulted in a 90% yield from HA-WI. The structural features of the sulfated- HA (S-HA) were investigated by FT-IR and ¹H-NMR spectroscopy. The FT-IR and NMR patterns revealed the similarity in both the FTIR spectrum as well as NMR spectrum of both reference standard and purified HA from S. dysgalactiae. The anti-inflammatory activities of HA and S-HA were examined on LPS-induced RAW 264.7 cells. S-HA was significantly inhibited production of pro-inflammatory mediators such as nitric oxide (NO) and PGE₂ and the gene levels of iNOS and COX-2, which are responsible for the production of NO and PGE₂, respectively. Furthermore, S-HA also suppressed the overproduction of pro-inflammatory cytokine TNF-α (<80 pg/ml) and IL-6 (<100 pg/ml) compared to that of HA-WI. The present study clearly demonstrates that HA-S exhibits anti-inflammatory activities in RAW 264.7 macrophage cells.

이종재료 접합부 선단의 크랙 전파 거동

국정한,성호현,송일규 한국기술교육대학교 1997 論文集 Vol.4 No.1

In this study, the crack behavior around the bonded dissimilar materials is analyzed by using by Finite Element Method(FEM) in case that it approaches or passes through the bonded line perpendicularly. The analysis criterion is based on the energy(△U) when the crack progresses or passes through the bonded line. The variables are the width of dissimilar material and distance from the crack tip to the bonded line. Values of E(Young's modulus), V(Poisson's ratio) and △U(Energy) for base material are different from those(E', V', △U') for dissimilar material. The outcome of this study is obtained by comparing △U with △U'

SS400강 용접부의 파괴인성치에 관한 연구

국정한,송일규,김봉중,오세용,성호현 한국기술교육대학교 1999 論文集 Vol.6 No.1

In this work, we studied the mechanical properties, structure and the stress intensity factor of the welded areas which were annealed from 500。C to 900。C with an interval of 100。C. The main results are as follow. 1. The as-welded specimen showed the highest tensile strength and yield point. The tensile strength and the yield point decrease as the annealing temperature increased from 500。C to 900。C, and they became smaller in the order of deposited metal, heat affected zone and base metal. 2. The hardness was maximum at about 9 mm from the welding center line, and it decreased drastically in the heat affected zone located between 9∼15mm from the center line. 3. The elongation decreased in the order of base metal, the fusion zone and heat affected zone while it increased as annealing temperature increased. 4. The stress intensity factor(S. I. F.) declined as the length of notch increased and it became smaller in the order of fusion zone, base metal and heat affected zone. This is because fracture toughness is affected by brittlization at heat affected zone as well as the initiation and propagation of cracks.

폐혈관상이 유지된 선천성 낭성 선종양 기형

배일헌,한기석,차상훈,김성진,박길선,이건국,홍장수 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

선천성 낭성 선종양 기형은 태생 16주 이후 말단세기관지의 형성이 중단되어 미성숙기관지들과 중배엽성분들이 과도하게 증식하여 발생하는 드문 선천성 폐질환이다. CT소견은 낭종의 크기 숫자, 내용물 등에 따라 다양하나, 병변내에 정상적인 폐혈관이 보인 예는 전세계적으로 보고된 적이 없었다. 저자들은 역동적 흉부 CT상 균일한 저음영의 병변내에 정상 폐혈관상이 유지된 선천성 낭성 선종양 기형 1예를 경험하였기에 보고한다. Congenital cystic adenomatoid malformation(CCAM) is a rare pulmonary disease that is thought to be developed by abnormal proliferation of immature alveoli and other mesenchymal component from the abrupt halting of the terminal alveoli formation after 16 weeks of gestation. On CT scan, there are no consistencies regarding to size, numbers, and its contents but no cases were reported in past showing normal pulmonary vascularities. We reports a case of CCAM that showed normal pulmonary vascularities within the hypodense lesions on a dynamic chest CT

소성변형 부위에서의 피로크랙전파

성호현,송일규,국정한 한국기술교육대학교 1998 論文集 Vol.5 No.1

In this study, the behavior of a fatigue crack propagation near the expanded hole was investigated in terms of the plastic deformation and position of hole with respect to the propagating crack tip. The results of this study are summarized as follows. 1. The crack propagation rate is high when the crack tip approaches the hole but the retardation effect becomes prolonged when the crack tip is at the very vicinity of the hole. 2. The proper expansion of the hole seemed to play an important role for the retardation of the fatigue crack propagation rate. It is found that 5% of plastic deformation in mild steel show the best effect of the fatigue crack retardation.

헬리코박터 파이로리 균 진단용 ^13C-요소 캅셀의 개발

용철순,김용일,김지만,강성훈,권기철,이종달,김종국,사홍기,최한곤 영남대학교 약품개발연구소 2002 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

The purpose of this study was to develop a new ^13C-urea-containing capsule for diagnosis of H.pylori. The urea-containing capsules were prepared with various diluents such as polyethylene glycol (PEG), microcrystaline cellulose, sodium lauryl sulfate and citric acid. The dissolution test, ^13C-urea breath test and stability test were then performed on the capsules. Microcrystalline cellulose and sodium lauryl sulfate retarded the initial dissolution rates of urea. However, PEG increased the initial dissolution rates of urea. Furthermore, two formulae composed of PEG,[^13C-urea/PEG (38/1.9 mg/cap)] and [^13C-urea/PEG (38/1.9/1.9 mg/cap)] had the maximum DOB value. about 16 at 20 min, while the formula composed of only 38 mg ^13C-urea had the maximum DOB value at 30 min. The results indicated that PEG improved the sensitivity of ^13C-urea in the human volunteers. The capsule [^13C-urea/PEG (38/1.9 mg/cap)] was stable for at least six months in 25 and 37℃. Thus, a PEG-containing capsule, [^13C-urea/PEG (38/1.9 mg/cap)] would be a more economical, sensitive and stable perparation for diagnosis of H. pylori.

분석결과 검증 및 신뢰성 확보를 위한 실험실간 협력 실험

박혜진,고광용,한국탁,김일중,이용재,김성헌,이규승 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.2

The residual study of pesticide has been used in various areas, such as food safety, environmental protection, establishment of tolerance, and explaining the pathway and reaction mode of pesticides, and its importance was expected to increase further more. The aspect of food safety, the pesticide residue survey have been practiced at many organizations, but there were no verification of analytical results at present. In this experiment, we focused on instrumental stability, including response of each instrument and the recovery ratio of each organization's method. As samples for this experiment, we prepared cucumber and sesame, and chose 4 pesticides (bifenthrin, chlorpyrifos, diazinon, and ethoprophos), which were mostly detected from pesticide residue survey and widely used for each crop. The standard deviation of peak areas in the chromatogram of each pesticide were under 1.212 %, so it showed that most instruments were stable. The relationship of recovery ratio of each organization were over 0.996 for every pesticide and each organization. Finally, the analytical results for pesticide residue from each participated organization were not statically significant and we could put confidence in the result from each organization.

^(99m)TC Phytate 간스캔에 있어서 정량적 간외섭취율의 임상적 의의

김병수,문태용,김건일,남상화,한국상 부산의대 1991 부산의대잡지 - 부산대학교 의과대학 Vol.31 No.2

Mission Hermeneutical Study on the Transformation of Culture

Kook-Il Han 장로회신학대학교 세계선교연구원 1999 선교와 신학 Vol.3 No.-