Salt-Induced Protein Precipitation in Aqueous Solution: Single and Binary Protein Systems

Kim, Sang-Gon,Bae, Young-Chan The Polymer Society of Korea 2003 Macromolecular Research Vol.11 No.1

A molecular-thermodynamic model is developed for the salt-induced protein precipitation. The protein molecules interact through four intermolecular potentials. An equation of state is derived based on the statistical mechanical perturbation theory with the modified Chiew's equation for the fluid phase, Young's equation for the solid phase as the reference system and a perturbation based on the protein-protein effective two body potential. The equation of state provides an expression for the chemical potential of the protein. In a single protein system, the phase separation is represented by fluid-fluid equilibria. The precipitation behaviors are simulated with the partition coefficient at various salt concentrations and degree of pre-aggregation effect for the protein particles. In a binary protein system, we regard the system as a fluid-solid phase equilibrium. At equilibrium, we compute the reduced osmotic pressure-composition diagram in the diverse protein size difference and salt concentrations.

교합면 우식병소의 다양한 진단법에 관한 비교연구

김재곤,김영진,김영신,백병주 大韓小兒齒科學會 2001 大韓小兒齒科學會誌 Vol.28 No.4

교합면의 열구는 우식이 발생하기 쉬운 위치이며, 특히 초기우식병소는 건전해 보이는 법랑질 하방에서 진행되는 경우가 많고, 주위 건전 법랑질과 잘 구별되지 않아서 주기적인 검사에도 불구하고 우식의 치료 시기를 놓치는 경우가 종종 있다. 초기 우식을 조기에 진단할 경우 불소도포, 식이조절, 치태조절 능력개선 그리고 타액분비 촉진 등의 방법으로 그 병리적 과정을 차단하고, 조기에 치료할 수 있다는 관점에서 조기 진단은 중요시 되고 있다. 본 연구는 발거된 치아에서 교합면 우식을 검출하기 위해 두가지 전통적인 진단법인 시진, 방사선 검사법에 대하여 레이저 형광측정법을 이용한 새로운 우식진단기구인 Diagnodent (Biberach , Germany)의 민감도, 특이도 그리고 정확도를 비교함으로서 교합면 초기 우식 병소에 대한 가장 신뢰성 있는 진단 방법에 대하여 평가하고자 하였으며 다음과 같은 결론을 얻었다. 1. Diagnodent는 건전치에서 7.8, 초기우식에서 25.4, 법랑질 우식에서는 30.5 그리고 상아질 우식에서는 53.8의 평균값을 나타냈다. 2. 염료를 이용한 치아조직표본검사와 스피어만과 피어슨 상관계수에서 Diagnodent(0.736, 0.619)와 시진(0.664, 0.666)에서 높은 상관성을 나타냈고 방사선 촬영검사(0.333, 0.335)에서 가장 낮은 상관성을 나타냈다(P<0.01, 전체). 3. 교합면 우식진단의 정확도는 Diagnodent의 민감도와 특이도가 가장 높았고 방사선 검사가 34%로 가장 낮았다. 4. 초기 우식의 경우, Diagnodent의 민감도와 특이도가 가장 높았고 법랑질 우식의 경우, 시신의 민감도가 가장 높았으며 Diagnodent의 특이도가 가장 높았다. 상아질 우식의 경우, Diagnodent의 민감도와 특이도가 가장 높았으며 시진의 민감도가 가장 낮았다. The aims of this study were to compare the accuracy, sensitivity and specificity of cnventional visual examination, radiography and a new laser fluorescence method, KaVo Diagnodent, for the detection of occlusal caries lesions. One hundred sound human premolars and molars which had no restorations or interproximal cavities were tested by three methods. Tooth lesions depth was assessed at histologic examination using Caries detector dye The following results were obtained. 1. Diagnodent show 7.8 in sound tooth, 25.4 in initial caries, 30.5 in enamel caries, and 53.8 in dentin caries with average score 2. Spearman and Pearson relation coefficient was high between tooth-specimen test with dye and Diagnodent(0.736, 0.619), visual examination(0.664, 0.666), and was low between tooth-specimen test with dye and radiographic examination(P<0.01, total) 3. Accuracy of occlusal caries was highest on Diagnodent(65%) and lowest on radiographic examination(35%) 4. In initial caries, the sensitivity and specificity of Diagnodent method was the highest. In enamel caries, the sensitivity of visual examination was the highest and specificity of Diagnodent method was the highest. In dentinal caries, the sensitivity and specificity of Diagnodent method was the highest and sensitivity of visual examination was the lowest.

남자 알코올 의존 환자의 기질적 특성에 관한 연구

김성곤,민영기,이덕기,김미영,송영상,권도훈,고영훈,박제민,정병선 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.1

목 적: 본 연구의 목적은 한국인 남자 알코올 의존 환자의 기질적 특성을 Temperament and Character Inventory(TCI)를 이용하여 조사하는 것이다. 방 법: DSM-Ⅳ 진단 기준에 맞는 알코올 의존 환자 30명과 정상인 36명을 대상으로 연구학적 특성을 조사하고, 기질적 특성을 한글판 TCI를 이용하여 조사하였다. 모든 대상자의 나이는 30~60세였다. 결 과: 1) 두 군간 평균 나이는 유의한 차이가 없었으나, 교육 정도와 결혼을 유지하고 있는 비율 및 취업률은 알코올 의존군이 정상 대조군에 비해 유의하게 낮았다. 2) 알코올 의존군에서는 정상 대조군에 비해 Harm Avoidance(HA) 점수와 Self-transcendence(ST)점수는 유의하게 높았으며, Self-directiveness(SD)점수는 유의하게 낮았다. 3) 알코올 의존군 내에서의 각 척도들 간의 상관관계는, HA와 SD 점수, HA와 Persistence(PE) 점수, ST와 SD 점수는 각각 유의한 음의 상관관계, ST와 PE 점수, SD 점수와 Cooperativeness(CO) 점수, Reward Dependence(RD)와 CO 점수는 양의 상관관계를 보였다. 결 론: 이러한 본 연구의 결과는 한국인 남성에서 수줍음을 잘 타며, 걱정도 많고, 염세적이고 쉽게 피로해지는 기질적 특성이 알코올 의존과 연관성이 있음을 의미한다. Objectives : The purpose of this study was to assess the temperament of male parients with alcohol dependence. Methods : The subjects were 30 patients who met DSM-Ⅳ criteria for alcohol dependence and 36 normal controls. All subjects completed Temperament and Character Inventory(TCI) which was developed by Cloninger. Results : 1) The educational level, marital status, and occupation in alcohol dependence group were significantly lower than those of normal control group, but there was no difference in the age between two groups. 2) Among the scores of TCI, the score of harm avoidance(HA) and self-transcendence(ST) were significantly higher in the alcohol dependence group than in the normal control group. 3) In the alcohol dependence group, a significant negative correlation among the scores of TCI was between HA and SD, HA and Persistence(PE), ST and SD, and positive correlation between ST and PE, SD and Cooperativeness(CO), Reward Dependence(RD) and CO. Conclusion : These results suggest that shy, fearful, pessimistic and fatigable temperament are related to developing alcohol dependence in Korean males.

HID 램프용 디지털제어 전자식 안정기에 관한 연구

김영동,강원찬,김남오,김형곤,김병철 조선대학교 에너지.자원신기술연구소 2004 에너지·자원신기술연구소 논문지 Vol.26 No.1

In this paper, digitally controlled electronic ballast for the HID lamps driven by proposed frequency conversion method is described. This electronic ballast consists of a buck converter, a low frequency square wave full bridge inverter, a high voltage pulse generator for the lamp ignition, an over current protection circuit and an 8-bit microcontroller. The fuzzy logic digital control operation is carried out by the 8-bit microcontroller. In spite of the limited control bandwidth caused by low operation speed of the 8-bit microcontroller, the good control performance for the constant lamp current in the warm-up period is obtained using the proposed frequency conversion driving method. The HID lamp is controlled in a constant current mode during the lighting warm up process and is controlled in a constant power mode during lighting steady state. A controlled variable power mode is used to extend the lamp lifetime.

日本의 社會科學 및 體育分野 學問硏究動向에 對한 調査 硏究

金榮秀,崔晄烈,金相漢,金麟坤,金炳斗,金永煥 慶北大學校 經商大學 經濟學科 1981 慶商大學論集 Vol.9 No.-

This paper attempts to review and trace back to, based on the data collected during visits of Japanese universities and research institutes, the organizational characteristics, the structures of curricula and study trends, with regard to the fields of social sciences, especially economics and business administration. One of the most conspicuous characteristics observed in academic organizations was Tsukuba university's system whose academic institution was organized around academic grouping, academic genealogy, and specialty: particularly social economic planning belonged to the genealogy of social engineering, in light of its characteristics in term of curriculum, seems to meet the contemporary need. There found two speciality areas in the Department of Social Engineering at the Graduate School: one is the concentration on Mathematical Planning and the other Management Engineering. Considering that its academic goal is tosys tematically and empirically analyze the human behaviors, and to take interdi-sci plinary approach through a policy-oriented system in doing so, it reflects one of the true natures of the academic trend. Their curricula appear to emphasize on analytical methods under uncertainty, application of mathematical planning techniques, system simulation, and information system design and its application, in the area of economics and business administration. Physical education is believed to require modernized facilities and equipments for its record-breaking and technical enhancement, and so increased investment in physical facilities and policy considerations for the training of physical educators are necessary.

소아 야뇨증에 대한 임상적 고찰

김승수,곽병곤,김영대,김우경,김영균,이종국 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Objectives: The aim of this study was to describe the clinical characteristics of enuretic children and to evaluate the efficacy of treatments. Methods: Our study subjects enrolled were 52 children with nocturnal enuresis, who visited outpatient clinic of pediatrics in Seoul Paik Hospital and Ilsan Paik Hospital, during the period from October 1998 to August 2004. The data of gender, age, family history, symptom, clinical type of enuresis and drug therapy were collected and analyzed retrospectively. Results: The male : female ratio was 2.25 : 1. The mean age at diagnosis was 6.8 years with an age range from 4.3 years to 12.5 years. Fourty five cases(86.5%) were diagnosed as primary enuresis and 7 cases(13.5%) were diagnosed as secondary enuresis. A family history of enuresis was noted in 5 cases(9.6%) including 4 cases with parental enuresis history. The accompanying urinary symptoms were urgency(11.5%), frequency (7.7%), and dysuria(2.0%). Conclusion: We found frequent occurrences of enuresis in boys and primary type and an occasional association with a family history of enuresis. The two main treatment modalities were behavior control with an alarm system and drug therapy with imipramine, desmopressin, or oxybutynin. We recommend continuous drug medications to control nocturnal enuresis along with encouragement.

플라즈마 이온주입기에서의 시변환 쉬스 연구

김곤호,김건우,김영우,최영도,김종식,김상진,한승희,홍문표 漢陽大學校 工學技術硏究所 1998 工學技術論文集 Vol.7 No.1

Abstract- In a plasma source ion implantation, the target is successively biased by negative voltage pulses with an intrinsic finite rise time, resulting in the time-dependent sheath formation around the target. Measurement of time-dependent sheath during the pulse rise show the ion matrix sheath development which is in proportion to square root of time and pulse rise rate over plasma density but independent of the ion mass.

배달 미니 게임 개발:"최고의 웨이터를 꿈꾸며"

김정훈,정한교,김영옥,김중곤,박정윤,윤진성,김경식 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 미니 게임은 웨이터라는 직업을 모티브로 하여서 남녀노소 누구나 재미를 느낌과 동시에 기억력 향상시켜 주는 게임제작이라는 목적아래 제작되었다.본 논문에서는 이 게임의 제작과정을 기술하고자 한다.이 게임은 제3회 AGC(한국 아마추어 게임제작 공모전)에서 우수 작으로 선정되었다.

한국인 알코올 의존 환자에서 알코올 대사 효소 유전자형 빈도의 남녀 차이

김성곤,김철민,이덕기,황인복,이현숙,김성연,전은숙,송영상,박제민,최병무,김명정 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.2

Objectives : There are a number of preceding epidemiological studies reporting gender differences in the genetic etiology of alcohol dependence. The author investigated gender difference in the frequencies of ADH2 and ALDH2 genoypes between the patients with alcohol dependence and normal control. Methods : The subjects were 141 alcohol dependent patients (104 males, 37 females) and 138 normal control (79 males, 59 females). The frequencies of 1/1 and 1/2+2/2 (2+ afterward) genotypes for ADH2 and ALDH2 were investigated in male and female between alcohol dependence and normal control group. DNA was extracted from WBC in peripheral venous blood and PCR-RFLP method was used out for genotyping. Results : First, the frequency of ADH2 1/1 genotype was significantly higher in alcohol dependent patients than normal control in both genders. Second, while there was no gender difference in the frequency of ADH2 1/1 genotype in normal controls, in the patient group however, the frequency was significantly higher in females than males. Third, in male subjects with alcohol dependence, the frequency of ALDH2 1/1 genotype was significantly higher than in male normal control subjects. On the other hand, in female subjects with alcohol dependence, the frequency of ALDH2 2+ genotype was significantly higher than in female normal control subjects. Conclusion : These results suggest that while the risk of alcohol dependence is predominantly affected by ALDH2 1/1 geno-type in male, the female ADH2 1/1 genotype is mainly associated with the risk of alcohol dependence. This means that there are gender differences in the genetic etiology of alcohol dependence.

자가 산부식 프라이머의 적용시간이 상아질의 미세인장 결합강도에 미치는 영향

조영곤,이영곤,김종욱,박병철,김종진,최희영,진철희,유상훈 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.5

This study evaluated the influence of application time of self-etching primers on microtensile bond strength (μTBS) to dentin using three self-etching primer adhesive systems. Dentin surfaces were exposed from forty-eight human molars. They were conditioned with three self-etching primers (Clearfil SE Bond [SE], Unifil Bond [UF], Tyrian SPE + One Step Plus [TY]) and different primining times (10s, 20s, 30s and 40s). Composite resins were bonded to dentin surfaces and specimens were made. μTBS was tested and statistically compared using by one-way ANOVA and Tukey's Test. The results of this study presented that priming time for 10s in SE and UF groups and for 30s and 40s in TY group was highly decreased μTBS to dentin.