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무주지역에서 채취한 산삼 생육 토양미생물에 대한 동정 연구
신동엽,박용일,김홍렬,안덕균,박성규 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2
objectives : Microorganisms were isolated from soils remaining attached to the roots of Wild Panax ginseng C.A. Mey. naturally grown in Moo-Ju area. methods : As a preliminary study for the establishment of wild ginseng radix discrimination system based on area of growth, these isolates were identified by using mainly API 20NE and API 50CHB kits and the microbial consortia were compared. From the sample of Moo-Ju area, 9 and 6 strains were isolated and identified after the incubation of agar plates at 30℃ and 37℃, respectively. results : The results showed that 2 strains of soil bacteria, Aeromonas hydrophilia, and Bacillus cereus commonly existed in the roots of wild ginseng of both 30℃ and 37℃. And that 6 strains, Pseudomonas putida, Stenotrophomonas maltophilia, Pseudomonas fluorescence at 30℃ and Bacillus subtilis, Empedobacter brevis, Brevi. laterosporus at 37℃, inhabited specifically in the roots of wild ginseng of Moo-Ju area. conclusion : These results suggest that comparison of microbial consortia associated with roots of wild ginseng could be used for the establishment of a naturally growing wild ginseng radix discrimination system.
Kim, Hong-Yeoul,Shin, Dong-Youp,Park, Yong-Il,Ahn, Duk-kyun,Park, Seong-Kyu INSTITUTE OF ORIENTAL MEDICINE KYUNG-HEE UNIVERSIT 2002 JOURNAL OF ORIENTAL MEDICINE Vol.7 No.1
Microorganisms were isolated from soils remaining attached to the roots of Wild Panax ginseng C.A Mey (Sansam) which were collected from Hongcheon province, Korea. As a preliminary study for the establishment of wild panax ginseng radix discrimination system based on area of growth, these isolates were identified by using mainly API 20NE and API 50CHB kits. From the soil sample, 3 microbial strains, Aeromonas hydrophilia, pseudomonas fluorescens, and Burkholderia cepacia, were isolated and identified after the incubation of agar plates at 30℃, and 3 different strains, Bacillus subtilis, Aeromonas hydrophilia, and Bacillus mycoides, were isolated and identified after the incubation of agar plates at 37℃. Among 4 strains initially isolated from the plates incubated at 37℃, two of them turned out to be the same species, Aeromonas hydrophilia. And also, Aeromonas hydrophilia was commonly isolated from both 30 and 37 culture plates.
Kim, Mi-Hwa,Kim, Hong-Beum,Acharya, Samudra,Sohn, Hong-Moon,Jun, Jae Yeoul,Chang, In-Youb,You, Ho Jin American Society for Microbiology 2009 Molecular and cellular biology Vol.29 No.8
<B>ABSTRACT</B><P>Apurinic/apyrimidinic endonuclease 1 (Ape1/Ref-1) dysregulation has been identified in several human tumors and in patients with a variety of neurodegenerative diseases. However, the function of Ape1/Ref-1 is unclear. We show here that Ape1/Ref-1 increases the expression of glial cell-derived neurotropic factor (GDNF) receptor α1 (GFRα1), a key receptor for GDNF. Expression of Ape1/Ref-1 led to an increase in the GDNF responsiveness in human fibroblast. Ape1/Ref-1 induced <I>GFRα1</I> transcription through enhanced binding of NF-κB complexes to the <I>GFRα1</I> promoter. GFRα1 levels correlate proportionally with Ape1/Ref-1 in cancer cells. The knockdown of endogenous Ape1/Ref-1 in pancreatic cancer cells markedly suppressed GFRα1 expression and invasion in response to GNDF, while overexpression of GFRα1 restored invasion. In neuronal cells, the Ape1/Ref-1-mediated increase in GDNF responsiveness not only stimulated neurite outgrowth but also protected the cells from β-amyloid peptide and oxidative stress. Our results show that Ape1/Ref-1 is a novel physiological regulator of GDNF responsiveness, and they also suggest that Ape1/Ref-1-induced GFRα1 expression may play important roles in pancreatic cancer progression and neuronal cell survival.</P>
Communication : Antibacterial effect of Scutellariae Radix on pathogenic bacteria
(Hong Yeoul Kim),(Kwang Ho Kim),(Mi Jung Yeom),(Dae Hyun Hahm),(Eun Su Lee) 경희대학교 한의학연구소 2000 JOURNAL OF ORIENTAL MEDICINE Vol.5 No.1
Antibacterial effects on Salmonella typhimurium, a representative enteric pathogen by the extracts of Scutellariae Radix(Scutellaria baicalensis Georgi) and its major flavonoid components - Baicalin, Baicalein - were studied. Through Disc analysis, growth
Antibacterial effect of Scutellariae Radix on pathogenic bacteria
Kim, Hong-Yeoul,Yeom, Mi-Jung,Hahm, Dae-Hyun,Lee, Eun-Su,Kim, Kwang-Ho INSTITUTE OF ORIENTAL MEDICINE KYUNG-HEE UNIVERSIT 2000 JOURNAL OF ORIENTAL MEDICINE Vol.5 No.1
Antibacterial effects on Salmonella typhimurium, a representative enteric pathogen by the extracts of Scutellariae Radix(Scutellaria baicalensis Georgi) and its major flavonoid components - Baicalin, Baicalein - were studied. Through Disc analysis, growth inhibition activity of Scutellariae Radix against S. typhimurium was found to be compatible with the commercial antibiotics such as ampicillin, tetracyclin, chloramphenicol, kanamycin. In addition, Disc analysis and minimum inhibitory concentration of Scutellaria Radix were investigated on various pathogenic bacteria such as Shigella sonnei, Klebsiella pneumoniae, and Proteus vulgaris. Interestingly, the antibacterial effects seem to be only acting on pathogenic bacteria rather than normal non-pathogenic bacteria such as E. coli. These results suggest that the Scutellia Radix work as specific natural antibacterial substrate on pathogenic bacteria.
( Minji Kim ),( Kim Won-baek ),( Kyoung Yoon Koo ),( Bo Ram Kim ),( Doohyun Kim ),( Seoyoun Lee ),( Hong Joo Son ),( Dae Youn Hwang ),( Dong Seob Kim ),( Chung Yeoul Lee ),( Heeseob Lee ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.4
This study was conducted to evaluate the hyaluronidase (HAase) inhibition activity of Asparagus cochinchinesis (AC) extracts following fermentation by Weissella cibaria through response surface methodology. To optimize the HAase inhibition activity, a central composite design was introduced based on four variables: the concentration of AC extract (X<sub>1</sub>: 1-5%), amount of starter culture (X<sub>2</sub>: 1-5%), pH (X<sub>3</sub>: 4-8), and fermentation time (X<sub>4</sub>: 0-10 days). The experimental data were fitted to quadratic regression equations, the accuracy of the equations was analyzed by ANOVA, and the regression coefficients for the surface quadratic model of HAase inhibition activity in the fermented AC extract were estimated by the F test and the corresponding p values. The HAase inhibition activity indicated that fermentation time was most significant among the parameters within the conditions tested. To validate the model, two different conditions among those generated by the Design Expert program were selected. Under both conditions, predicted and experimental data agreed well. Moreover, the content of protodioscin (a well-known compound related to anti-inflammation activity) was elevated after fermentation of the AC extract at the optimized fermentation condition.
국내 HIV 감염 / AIDS 의 역학적 및 임상적 양상
김준명(June Myung Kim),조군제(Goon Je Cho),홍성관(Sung Kwan Hong),정주섭(Ju Seub Chung),장경희(Kyung Hee Jang),김창오(Chang Oh Kim),박윤수(Yoon Soo Park),조정호(Jung Ho Cho),김효열(Hyo Yeoul Kim),최영화(Young Hwa Choi),송영구(Young G 대한내과학회 2001 대한내과학회지 Vol.61 No.4
N/A Background: The epidemiologic and clinical features of HIV infection/AIDS are different among various races, regions, and countries. To determine the epidemiologic and clinical characteristics of HIV infection in Korea, we analyzed and compared with that of other populations. Methods: Medical records of 176 HIV-infected per sons in Severance Hospital of Yonsei University College of Medicine and Hospital of Pusan University College of Medicine from year 1985 to 2000 were reviewed retrospectively. Results: One hundred and seventy six patients were analyzed among which 156 (88.6%) were male and 20 (11.4%) were female with a male to female ratio of 7.8:1. At the time of diagnosis, the age distribution was 78 cases (44.3%) in the thirties, 44 cases (25.0%) in the twenties, and 35 cases (19.9%) in the forties, and the mean age was 35.9±9.3. Heterosexual contact was the most frequent transmission route (92 cases, 52.3%), and 42 cases (23.9%) were transmitted by homosexual contact. At initial visit , asymptomatic HIV infection constituted 75 cases (42.6%), and AIDS 72 cases (40.9%). At initial visit, mean value of CD4+ lymphocyte counts was 252/mm3 and HIV RNA 226,035 copies/mm3. One hundred and twenty one of 176 patients developed 317 cases of opportunistic diseases. At the diagnosis of HIV-related opportunistic diseases, mean CD4+ lymphocyte count was 140/mm3 and mean HIV RNA 347,403 copies/mm3. Candidiasis (50 cases, 28.4%) was the most frequent opportunistic disease followed by pneumocystis carinii pneumonia (PCP) (37 cases, 21.0%), tuberculosis (29 cases, 16.5%), cytomegalovirus (CMV) infection (21 cases, 11.9%), HIV encephalopathy (9 cases, 5.1%), and herpes zoster (9 cases, 5.1%). There were 3 cases (1.7%) of malignant lymphoma and 2 cases (1.1%) of Kaposi's sarcoma. At the diagnosis of opportunistic diseases, mean CD4+ lymphocyte counts of patients with candidiasis was 71/mm3, PCP 63/mm3, and tuberculosis 142/mm3, and the mean HIV RNA level was 338,474 copies/mm3, 281,967 copies/mm3, and 817,012 copies/mm3 respectively. Among the 317 opportunistic diseases, AIDS-defining diseases were 150 cases (47.3%), of which PCP was 37 cases (24.7%), tuberculosis 29 cases (19.3%), CMV infection 21 cases (14.0%), HIV wasting syndrome 15 cases (10.0%), and esophageal candidiasis 14 cases (9.3%). The earliest AIDS-defining diseases to manifest in AIDS patients were tuberculosis (25 cases, 33.3%), followed by PCP (17 cases, 22.6%), esophageal candidiasis (14 cases, 18.7%), CMV infection (5 cases, 6.6%), and HIV wasting syndrome (4 cases, 5.3%). Thirty-five (19.9%) of 176 patients were died. The common causes of death were tuberculosis (9 cases, 25.7%), PCP (9 cases, 25.7%), bacterial pneumonia (7 cases, 20.0%) and HIV encephalopathy (3 cases, 8.5%). Conclusion: The epidemiologic and clinical features of HIV infection/AIDS in Korea are different from that of developing countries such as Southeast Asia and Africa as well as from that of developed countries. (Korean J Med 61:355-364, 2001)
Gas Chromatography-Mass Spectroscopy를 이용한 γ-Linolenic Acid(GLA) 생성 균주탐색 및 확인
김정봉 ( Jung Bong Kim ),김경환 ( Kyung Hwan Kim ),홍승범 ( Seung Beom Hong ),박종석 ( Jong Sug Park ),이종렬 ( Jong Yeoul Lee ),김삼선 ( Sam Sun Kim ),배신철 ( Shin Chul Bae ),조강진 ( Kang Jin Cho ),이동진 ( Dong Jin Lee ) 한국균학회 2007 韓國菌學會誌 Vol.35 No.2