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국내 약용자원식물의 지방산 조성과 ${\gamma}-linolenic$ acid 탐색
김정봉,김용환,이철희,황영수,박노동,Kim, Jung-Bong,Kim, Young-Hwan,Lee, Cheol-Hee,Hwang, Young-Soo,Park, Ro-Dong 한국약용작물학회 1995 한국약용작물학회지 Vol.3 No.2
Fatty acid composition analysis was carried out from 60 species of Korean native medicinal plant to screen ${\gamma}-linolenic$ acid(GLA) resources. Oenothera odorata, SymPhytum officinal, and Lithospermum erythrorhizon contained ${\gamma}-linolenic$ acid with 9.5%, 3.5%, 7.2% of total fatty acid content, respectively. The vegetative organs usually contained higher level of ${\alpha}-linolenic$ acid than seeds. Some plants were found to be excellent source of unsaturated fatty acid. 우리나라에서 생산되고 있는 60여종의 약용식물의 지방산 조성을 조사하여 식물체의 부위 와 종류에 따른 지방산 조성의 특성을 관찰한 결과 아래와 같은 결과얻었다. 1. 달맞이꽃종자, 컴프리잎, 지치뿌리에서 각각 총지방산함량의 9.1, 3,5, 7,2%의 GLA가 검출되었으며 달맞이 꽃종자가 컴프리잎이나 지치뿌리보다 GLA의 절대량이 많았다. 2. 들깨종자, 참깨종자, 호박종자등의 지방산이 양(量)과 불포화도면에서 좋은 자원임이 확인되었다. 3. 식물체의 잎, 줄기, 뿌리에서의 지방산조성은 종자의 경우에 비하여 18 : 3의 분포 비율이 현저히 높게 나타났으며 18 : 0의 비율은 비교적 같게 나타났다.
김정봉 ( Jung Bong Kim ),김민희 ( Min Hee Kim ),이영이 ( Young Yi Lee ),이호선 ( Ho Sun Lee ),이석영 ( Sok Young Lee ),김헌웅 ( Heon Woong Kim ),추상미 ( Sang Mi Chu ),백형진 ( Hyung Jin Baek ),조영숙 ( Young Sook Cho ),김재현 ( J 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.3
A methodological study was conducted to test the potential of near-infrared reflectance spectroscopy (NIRS) for the estimation of fatty acid composition and content in sesame seeds. A set of 3237 sesame lines, provided from the Rural Development Administration, were scanned by NIRS for their fatty acids. Calibration equations for the content of fatty acids were developed for the 122 lines of them by GC-FID(gas chromatography-flame ionization detector). Main fatty acids were palmitic acid(C(16:0), 8.5%), stearic acid(C(18:0), 5.7%), oleic acid(C(18:1), 39.3%), linoleic acid(C(18:2), 43.4%), and linolenic acid(C(18:3), 0.4%); the lowest and highest level were IT028899-7.2% and IT169359-10.0% in palmitic acid and IT028897-4.5%, IT028910-7.0% in stearic acid, IT170020-32.2% and IT156371-46.5% in oleic acid, IT156348-36.5 and IT169926-49.6% in linoleic acid, and IT169316-0.2% and IT156367-0.6% in linolenic acid, respectively. Determination of the fatty acid composition of seeds could be carried out successfully by using the spectra patterns of NIRS for both oleic and linoleic acids. As a result, oleic acid and linoleic acid compositions(% of total fatty acid) ranged from 25.8% to 49.2% and from 21.7% to 52.2%, respectively, in sesame seeds. Correlation(r²) between the percentage of oleic acid and linoleic acid was calculated in (-0.23).
Gas Chromatography-Mass Spectroscopy를 이용한 γ-Linolenic Acid(GLA) 생성 균주탐색 및 확인
김정봉 ( Jung Bong Kim ),김경환 ( Kyung Hwan Kim ),홍승범 ( Seung Beom Hong ),박종석 ( Jong Sug Park ),이종렬 ( Jong Yeoul Lee ),김삼선 ( Sam Sun Kim ),배신철 ( Shin Chul Bae ),조강진 ( Kang Jin Cho ),이동진 ( Dong Jin Lee ) 한국균학회 2007 韓國菌學會誌 Vol.35 No.2
청갓과 적갓에 함유된 Glucosinolates의 항암 활성 및 정량 분석
김활 ( Hwal Kim ),김준열 ( Jun Yeol Kim ),김효진 ( Hyo Jin Kim ),김도경 ( Do Kyung Kim ),조혜진 ( Hye Jin Jo ),한병수 ( Byoung Su Han ),김헌웅 ( Heon Woong Kim ),김정봉 ( Jung Bong Kim ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.3
The objective of this study was to compare the anticancer activity of glucosinolates against four different cancer cells; SNU-251, SNU-354, SNU-C4, MCF-7 and to determine the amounts of glucosinolates in mustard leaves. Green and red mustard leaves were cultivated on the field of Rural Development Administration from May to July, 2010. After the leaves were harvest and lypophilized, the fine powder was extracted with boiling 70% methanol(v/v) for the measurement of anticancer activity and then applied to the cancer cells obtained from Korean Cell Line Bank(KCLB). The anticancer activity of red leaf mustard was higher than that of green mustard leaf, particularly in SNU-251 for 24 hr and in SNU-C4 for 48 hr. In both green and red mustard leaf, sinigrin was the most abundant glucosinolte and was determined as 4.71 and 3.06 ㎎/g dry wt., respectively, but glucoiberin only presented 0.14 ㎎/g dry wt. with minor amounts.
LC-DAD-ESI/MS를 이용한 국내 자색벼 품종 종실의 안토시아닌 조성 및 함량 분석
김헌웅 ( Heon Woong Kim ),김정봉 ( Jung Bong Kim ),추상미 ( Sang Mi Chu ),김소영 ( So Young Kim ),김세나 ( Se Na Kim ),조영숙 ( Young Sook Cho ),조수묵 ( Soo Muk Cho ),백형진 ( Hyung Jin Baek ),박홍주 ( Hong Ju Park ),김재현 ( Jae 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.3
The content and composition of anthocyanin were determined in grains of the Korean purple rice varieties using liquid chromatography with diode array detection and electrospray ionization/ mass spectrometry (LC-DAD-ESI/MS). Isolated individual anthocyanins were identified and confirmed as cyanidin 3,5-diglucoside, cyanidin 3-glucoside, peonidin 3-glucoside and unknown compound with cyanidin based by interpretation MS fragments. Among them cyanidin 3-glucoside and peonidin 3- glucoside were major components. Especially, cyanidin 3-glucoside showed highest proportion and the anthocyanin contents were found to be significantly higher in Heugjinjubyeo(408mg/100g) than in other variety.