Effects of Chungshimgunbi-tang on the Expressions of Tissue Factor and the Plasminogen Activator Inhibitor-1 Gene

Ko,Chang-Nam,Cho,Ki-Ho,Kim,Hong-Soon,Kim,Young-Suk,Lee,Kyung-Sup,Moon,Sang-Kwan,Bae,Hyung-Sup 慶熙大學校韓醫科大學韓醫學硏究所 1999 慶熙韓醫大論文集 Vol.22 No.1

Tissue factor와 PAI-1 유전자는 혈전증에 있어서 중요한 작용을 한다고 알려져 있다. 혈전증의 원인에 대해서 아직 자세히 알려져 있지는 않지만 protease 들의 응고 cascade의 일차적인 inhibitor이며 세포표면의 plama factor Ⅶ/Ⅶa의 receptor 및 co-factor로 알려진 tissue factor와 plasminogen activator를 저해하여 fibrin clot의 제거를 방해하는 것으로 알려진 plasminogen activator inhibitor-1 (PAI-1)이 관여하고 있다고 보고되고 있다. 이에 따라 본 연구에서는 한의학 임상에서 항혈전증에 빈용되는 청심건비탕이 Tissue factor와 PAI-1 유전자에 미치는 영향을 RT-PCR 방법을 사용하여 연구하였으며 이와 동시에 항혈전증이 기대되고 있는 가미형지탕, 가미이기탕, 가미정기산 및 가미소척탕등의 4개 처방도 screen하였다. 또한 그 세부방법으로 Tissue factor 유전자의 발현에 중요한 전사조절제인 NF-??B와 PAI-1 유전자의 발현에 중요한 AP-1 의 활성도를 chloramphenicol acetyltransferase (CAT) assay로 검증하였으며, Electrophoretic mobility shift assay로 NF-kB와 AP-1의 DNA와 결합을 조사하였다. 이상의 방법으로 청심건비탕이 혈전증 유발에 중요하다고 알려진 tissue factor와 plasminogen activator inhibitor-1 (PAI-1) 유전자의 발현에 대한 영향을 검토한 바 다음과 같은 결론을 얻었다. 1. 청심건비탕은 인간 monocyte cell line U937에서 tissue factor 와 PAI-1 mRNA의 생성을 농도 의존적으로 감소시켰으며 다른 약재들은 큰 차이를 보여주지 않았다. 2. NF-kB와 AP-1 의 활성도를 chloramphenicol acetyltransferase (CAT) assay로 검증한 결과 청심건비탕은 이들의 활성도를 유의하게 감소시켰다. 3. Electrophoretic mobility shift assay로 NF-kB와 AP-1의 DNA와 결합을 검증한 결과 청심건비탕은 이들 전사촉진제들이 DNA와 결합하는 것을 저해시킴이 규명되었다.

漢醫學에서의 黃疸의 病理에 關한 文獻的 考察

安圭錫,文濬典 慶熙大學校韓醫科大學韓醫學硏究所 1978 慶熙韓醫大論文集 Vol.1 No.-

韓醫學과 西洋醫學의 疾病觀에 대한 比較硏究

白上龍,朴贊國 慶熙大學校韓醫科大學韓醫學硏究所 1998 慶熙韓醫大論文集 Vol.21 No.1

In the first step that medicine had became a study, a disease ment the pain that people suffered, in both the Oriental medicine(韓醫學) and the Western medicine. But the Oriental medicine and the Western medicine have typical opinion each other in the progress of sysytematizing the medicine. And, the reason that their cognition of the disease has been changed stems from the differences of view of the world, and the element of culture and society, which belongs to concerned scholars. The ideology and theory of ancient chineses who are considered as a constructors of the Oriental medicine's basis are formed from the attitude to adjust and accept the changes in the nature according to senses they feel. To adjust the nature, understanding of nature's rule was their priority. 'Yin-Yang and Five-phase theory(陰湯五行理論)' as systematized as a principle by recognizing nature's rule through those effects, and qi(氣) is appeared. which help everything exist as a perfect indivisual. And, they regarded continuously various changes of nature is due to the change of qi under the 'Yin-Yang and Five-phase theory'. For creature like a man is regarded as a change of qi, according to the rule of 'Yin-Yang and Five-phase theory', also the soul controling lives was regarded as temporary result revealed in the process of the change in qi's Yin-Yang and Five-phase(陰陽五行). that view of nature is established as a universal ethics to nature and human through a book, 'the Hwang-Ge-Nae-Kyung(黃帝內經)' and forms the basis theory of the Oriental medicine. Early the Western medicine differs from present one. The 'Body fluid theory(體液學說)' of early the Western medicine, represented by Hippocrates and Galen, tried to get an answer to the physiology and pathology through the growth of body fluid. In other words, it regards man's secretion or body wound or anatomy as a cause of disease and living as well. This body fluid correspond to the qi, considered as the origin of a life in the Oriental medicine, in that it is movable and unclear in each domain. The similarity between qi and body fluid, movable and changable, is the main reason that the surgery has not developed within these two theories. But, though body fluid is something visible and is changable in condition of liquid, it doesn't have such features as qi that can variously change into solid, liquid, energy, soul, and so on. So to speak, qi contains all imaginable features of objects in its conception. In contrast, body fluid is only a fluid that can control the physiological function. The Oriental medicine regards the disease as disorder of qi, also body fluid pathology says that the disease is a disorder of body fluid. Thus, both has consistent opinion about disease in the point of disorder. O.M.D(韓醫學者) thought the all disorder of mind and body, disorder between mind and body, even a change in nature were caused by the change of qi. But, because body fluid pathology couldn't explain that such organization as human, animal, and plant has a relationship with another objects. It could define a disease as a matter phenomenon like disorder of body fluid, but couldn't reach to find the consistency among the various objects in nature. Since the Oriental medicine thought of disease as breakage of Yin-Yang balance(陰陽調和) in other words abnormal function. Four great doctor in Kum and Won dinasty(金元四大家) tried to understand feature of disease, which caused a disease, and pathogenesis by explaining sa-qi(邪氣 bad effect making body function disorder) and peculiarity of patient's body rather than disease itself. Ha-Ghan(河間), who understood pathological mechanism through etiology itself, explained the process and feature of disease. In contrast Won-So(元素) and Dong-Won(東垣), who tried to understand the pecularity of disease through physiologycal mechanism tried to understand pathologycal mechanism between living energes, so to speake Wui-qi(胃氣), and 'yum-hwa(陰火)' which generates in abnormal function. The the Western medicine faced the new wave from the late of 17C, and then in paris where often the new era of clinical medicine. Solid pathology generally came to the front of fluid pathology with Bichat insistance that disease is not invalance of body fluid but chance of original shape and quality in late 18C. In solid pathology disease is not regarded as abnormally quantative and functional condition but as change of original shape and quality. This view is based on Descartes and newton's 'machanic view of the world'(機械的世界觀), which converted the way of western thinking since 17C. and the feature of this view regards disease as an object itself, and separate disease from a patient. The disease degraded over different nature which has none relative with living creature.

韓醫學과 隣接學問과의 協同硏究에 對한 考察 : 非韓醫界의 碩 ·博士 學位論文 中 韓醫學 關聯 論文에 對한 分析을 通하여

安秉哲,徐廷撤 慶熙大學校韓醫科大學韓醫學硏究所 1998 慶熙韓醫大論文集 Vol.21 No.1

In the present study, 284 of dissertations for the master's or doctor's degree on oriental medicine, which had been submitted to non-oriental medical field and, were collected, investigated and summarized as follows. 1. On the whole, the number of the dissertations of non-oriental medical field on oriental medicine was much more than that of oriental medicine field with larger number of dissertations for master's degree than for doctor's degree. 2. The subjects which have been consulted most frequently were constitutional medicine and acupuncture and moxibustion. 3. Research on health cited oriental medicine most frequently. The topic most frequently used was 'actual condition of the utilization of oriental medicine' and there were several studies on the comparison between western medicine and oriental medicine. 4. Theses of pharmaceutics studied oriental medicine second most frequently. Many of them were not on compound prescription but on simple recipe of herb. 5. There were less number of thesis on oriental medicine in medical field. Prejudice of medical field that the herbs have the hepatic-toxicity should be corrected, and further cooperative studies should be followed. 6. Most of the dissertations of dentistry studied on the effect of electro-acupuncture on Hapgok(LI4, 合谷) point. Other studies on oriental medicine should be followed. 7. The use of animal medel in veterinary medicine was prominent. This scientific method should also be applied in oriental medicine field. 8. There was no thesis on palm acupuncture in oriental medical field, whereas there were two in physical education field and one in administration field. Studies on palm acupuncture should be tried in order to make several useful acupuncture which have been applied as folk therapy included into oriental medical field. 9. Other fields also studied on oriental medicine with great effort. Further studies using genetics, molecular biology and information & communication engineering should be followed in order to develop oriental medicine.

Does Weather Change Cause Bell's Palsy?

Kim,Yong-Suk,Kang,Sung-Keel,Kim,Chang-Hwan,Koh,Hyung-Kyun,Park,Young-Bae 慶熙大學校韓醫科大學韓醫學硏究所 1995 慶熙韓醫大論文集 Vol.18 No.2

한의학에서는 말초성 안면신경마비가 주로 풍한의 침습으로 발병된다고 주장되고 있다. 이에 저자들은 말초성 안면신경마비와 기후변화와의 상관관계를 규명하고자 경희의료원 한방병원 1침구과에 내원한 서울거주 환자중 1994년 1년동안 발병한 463명(남자 227명, 여자 236명)의 환자를 대상으로 기상청에서 발간된 일별기상자료에 준하여 발병일 및 발병전 2일간의 평균기압, 평균기온, 최저-최고온도차이, 평균습도, 평균풍속, 평균운량(雲量) 및 일조시간을 분석하였다. 안면신경마비의 발병과 최고온도와 최저온도의 차이, 평균풍속, 평균기온, 평균일조시간, 평균운량 및 평균습도가 안면신경마비의 발병과 연관성이 있는 것으로 추정되었다. 이는 한의학의 발병원인과 부합되는 것으로 기후변화가 안면신경마비의 원인 중에 하나가 될 수 있다고 사려된다.

장세척의 한의학적 응용

김진성 慶熙大學校韓醫科大學韓醫學硏究所 1998 慶熙韓醫大論文集 Vol.21 No.1

What we explored so far are : the physiological functions, concerning the colon irrigation, of colon : the definition, the application and the methods; the colonic stasis after the colon irrigation : thc hazards of the colon irrigation : dobyunbeob(導便法) and boyuguanjang(保留灌腸). Colon irrigation is, as the research above presents, a colon cleansing system that involves the safe, gentle infusion of purified warm water into the colon. using no chemicals or drugs. Colon irrigation simply bathes the colon, removing impactions from colon walls, stimulating peristaltic action and enhancing the absorptive ability of the colon. This modality cleanses the colon from rectum to cecum. The application of this method has been limited mainly to colonoscopy, the pre-treatment of the operations on colon, the control of fecal continence after colostomy, the exclusion of fecal impaction from pediatric and geriatric patients and the whole gut irrigation as the method to treat constipation. However, it appears to be applicable to more diverse diseases including constipation and ulcerative colitis. 'Toxinon Unit' or 'PIEE(Pulsed Irrigation Enhanced Evacuation) System' can be used for these purposes. As Far as a suitable treatment is given, there should be no damage to the mucosa of colon. And, if the irrigating solution containing potassium chloride is used, there should be almost no loss of electrolytes and minerals. Of course, losing weight or the bio-chemical abnormality in blood is not as serious to be put in the discourse. However, there is a decrease of the number of the flora of which the function is important such as making the residuum be absorbed in colon. Since it is obvious that the decrease of the flora can be normalized by a way of diet, more research in this aspect is required. Any procedure which causes artificial distension or even abnormal irritation of the colonic mucosa can cause collapse. This effect is probably due to cardiac inhibition through a reflex mechanism, and it is not uncommon for normal individuals to faint or complain of sudden weakness, sweating and tachycardia after an enema, especially if this is run in too fast or too hat, or contains too strong an irrtant. If a due to have ulcerative colitis or diverticulitis is seen, more caution is required. It must be remembered that considerable amounts of fluid may be absorbed during colon irrigation and patients liable to edema who are on a restricted fluid intake present a particular risk. dobyunbeob(導便法), which appears in the classical texts of the Oriental medicine, is a kind of rectal injection to treat a severe constipation. And boyuguanjang(保留灌腸), which is a treatment of dropping decoction only or a concoction with a saline solution into anal in order to cure diseases in the rectum, is being applied to ulcerative colitis, chronic colitis, diarrhea, pediatric dysentery etc. Even though it seems to be more effective to boyuguanjang(保留灌腸) using colon irrigation machine, a research into the safety of the medicine to be put in anal should be preceded.

生肝溫脾湯이 TAA로 誘發된 흰쥐 肝損傷에 미치는 影響에 關한 硏究

鄭成忠,金秉雲 慶熙大學校韓醫科大學韓醫學硏究所 1985 慶熙韓醫大論文集 Vol.8 No.-

丹蔘水鍼이 鎭痛 및 血壓降下에 미치는 影響

宋春浩,朴東錫,姜成吉 慶熙大學校韓醫科大學韓醫學硏究所 1985 慶熙韓醫大論文集 Vol.8 No.-

Communication / An Evidence - Based Approch to Oriental Medicine : the Example of Acupuncture

(Edzard Ernst) 경희대학교 한의학연구소 1999 JOURNAL OF ORIENTAL MEDICINE Vol.4 No.1

仙遺糧湯 · 紫金錠 · 甘豆湯이 Hgcl₂ 中毒家兎의 腎 및 肝損傷에 미치는 影響

申正植,蔡炳允 慶熙大學校韓醫科大學韓醫學硏究所 1988 慶熙韓醫大論文集 Vol.11 No.-