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뇌수막종에 있어서 유세포 측정 양상과 임상적 형태와의 관계
김정훈,정영섭,김동규,정희원,김현집,최길수,한대희 대한신경외과학회 1990 Journal of Korean neurosurgical society Vol.19 No.6
Meningiomas have a wide range of biological potential and clinical behavior. Histological findings are helpful in recognizing the malignant potential of a given tumor, but often fail to correlat with clinical and biological behavior such as a gross feature, the liability of recurrence, and the extent of associated cerebral edema. To find alternate approaches to improve the correlation between clinical and biological behavoir, 18 meningiomas were studied by flow cytometry(FCM) using paraffin embedded tissues for deoxyribonucleic acid(DNA). The results are summarized as follows: 1) Fifteen cases were diploid and remaining three cases were aneuploid. The latter were two cases of an angioblastic meningioma, and one case of a malignant meningioma which recurred two years later. 2) No relationships were found between the results of ploidy analysis and the age, sex, the site of neoplasms, the size of neoplasms, the degree of peripheral edema, mitotic index, recurrence and the histological subtypes(p>0.1), but it was interesting to note that all three aneuploid cases were clinically and biologically aggressive meningiomas. 3) The proliferative index(PI : %S+%G₂M) between diploid and aneuploid cases had a statistically significant trend(0.05<P<0.1). 4) No significant differences were found between the age, sex, site, histological subtype, mitotic index, recurrence and the proliferative index value(p>0.1). 5) The PI value was relatively correlated with the size and the extent of associated cerebral edema(0.05<P<0.1). These data do not support the suggestion that flow cytometry in meningiomas may be of predictive value concerning the clinical behavior of these neoplasms, but the possible association between tumor aneuploidy, high PI value and poor clinical outcome in meningiomas merits further investigation.
AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants
Kang, CH,Jung, WY,Kang, YH,Kim, JY,Kim, DG,Jeong, JC,Back, DW,Jin, JB,Lee, JY,Kim, MO,Chung, WS,Mengiste, T,Kolwa, H,Kwak, SS,Bahk, JD,Lee, SY,Nam, JS,Yun, DJ,Cho, MJ Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
Calmodulin (CaM) influences many cellular processes by interacting with various proteins. Here, we isolated AtBAG6, an Arabidopsis CaM-binding protein that contains a central BCL-2-associated ethnogeny (BAG) domain. In yeast and plants, overexpression of AtBAG6 induced cell death phenotypes consistent with programmed cell death (PCD). Recombinant AtBAG6 had higher affinity for CaM in the absence of free Ca^2+ than in its presence. An IQ motif(IQXXXRGXXXR, where X denotes any amino-acid) was required for Ca^2+ -independent CaM complex formation and single amino-acid changes within this motif abrogated both AtBAG6-activated CaM-binding and cell death in yeast and plants. A 134-amino-acid stretch, encompassing both the IQ motif and BAG domain, was sufficient to induce cell death. Agents generating oxygen radicals, which are known to be involved in plant PCD, specifically induced the AtBAG6 transcript. Collectively, these results suggest that AtBAG6 is a stress-upregulated CaM-binding protein involved in plant PCD.
Pathological Analysis of Dysplastic and Malignant Nodules in 21 Explant Livers
Jung,ES,An,BM,Park,DH,Kim,BS,Choi,JY,Lee,CD,Park,YM,Han,NI,Byun,BH,Kim,BK,Bae,SH,Yoon,SK,Baek,JH,Byun,JY,Kim,DG,Kim,IC,Cha,SB 가톨릭중앙의료원 가톨릭암센터 1998 암심포지움 Vol.- No.2
동족 간이식이 보편화됨에 따라서 전체간을 분석할 수 있게 됨으로서 간암 및 전암병변에 대한 방사선학적, 병리학적 연구가 활발하게 진행되고 있다. 간경변증은 간암의 가장 중요한 선행질환으로서 이에 대한 병리조직학적 연구는 간암발생의 단계적 발전과정과 관련된 기전을 밝히는데 중요한 단서를 제공할 것으로 시사된다.
Benzo(a)pyrene과 Doxorubicin을 투여한 마우스 배아 섬유아 세포의 고농도 염으로 추출한 염색질 분획에서 DNA topoisomerase Ⅱ의 위치
김중근,이도근,조무연,정인철 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.5
마우스 배아 섬유아 C3H/10T1/2 세포의 핵 분획에서 DNA 절단과 재결합에 관여하는 DNA topoisomerase II의 부위를 측정하였다. 또한 benzo(a)pyrene (BP)과 doxorubicine(Dx)을 단독 혹은 동시 투여하고 배양한 세포에서, Northern blot hybridization으로 이들 약제에 의한 c-myc 유전자 발현을 측정하였다. 고농도 염으로 추출한 염색질 아분획에서 DNA topoisomerase II 활성도 변화를 관찰하고 토끼에서 제조한 효소 항체를 이용하여 Western blot immunodetection을 실시하여 다음과 같은 결과를 얻었다. 1. C3H/10T1/2 세포의 염색질 분획에 DNA topoi somerase II가 강하게 결합되어 있으며 고농도의 염 용액(2M NaCl)의 녹지 않는 분획(DNA-S, DNA-P)에 효소가 존재함을 확인하였다. 2. 시험관 내에서 pBR322에 BP의 농도별(1, 4, 10 μM) 투여에서 DNA topoisomerase II의 이완 운동이 증가하였다. 그러나 BP/Dx의 동시 투여에서는 효소 의 활성에는 변화가 없으나 운동 이동이 있음을 관찰하였다. 3. C3H/10T1/2세포의 c-myc 유전자 발현은 Nor thern blot 분석에서 Dx보다 BP 투여에서 더욱 증가가 있으나 BP/Dx의 동시 투여에서는 BP에 의한 유 전자 발현이 감소되었다. 4. BP와 Dx을 단독 또는 동시 투여한 C3H/10T1/2 세포에서, 고농도 염용액에 침전된 염색질의 분획, DNA-S와 DNA-P의 topoisomerase II 활성은 BP 투여 군에서 양쪽 분획의 모두에 나타나나 Dx 투여군에서 는 단지 DNA-S에만 활성이 있었으며, BP/Dx 투여군 에서는 DNA-S 분획이 BP단독 투여군 보다 높았다. 5. 상기 분획의 Western blot immunodetection에서 DNA topoisomerase II가 BP 투여군에서는 DNA-S와 DNA-P 분획에 존재하였으며 Dx 투여군에서는 DNA-S 분획에만 있으며 BP/Dx 투여군에서는 DNA-S에 더욱 많이 존재하였다. 이상의 결과는 고농도의 염용액에 녹지 않는 분 획이 DNA topoisomerase II의 활성을 나타내는 부위였으며, Dx의 작용에서는 DNA-S 분획이 유전자의 기능에 중요한 역할을 하는 것으로 보인다. This study was conducted to determine if there exists a DNA topoisomerase II participating in the cleave and rejoining of DNA in the nuclear fraction of a mouse embryo fibroblast, C3H/10T1/2 cells to measure the expression of c-myc gene in the cells cultivated with benzo(a)pyrene (BP) and doxorubicin (Dx), together or alone, by means of Northern blot hybridization, and to observe changes of DNA topoisomerase II activity in chromatin subfraction extracted with high salt solution, and to confirm the existence of DNA topoisomerase II by Western blot immunodetection using the DNA topoisomerase II-antibody from the rabbit. The results are summarized as follows: 1. There was a region in the nuclear fraction of the C3H/10T1/2 cell showing a strong DNA topoisomerase II activity, which was included in the fraction insoluble to high salt (2M NaCl) solution. 2. In in vitro experiments, the relaxation activity of DNA topoisomerase II in pBR 322 DNA was increased by treatment with different concentration (1, 4, 10μM) of BP similar results were obserbed with BP/Dx treatment, although there was some mobility shift. 3. In Northern blot analysis, c-myc gene expression was increased in C3H/10T1/2 cell treated BP or Dx, but it decreased in cells treated with BP and Dx together. 4. Centrifugation of 2M NaCl chromatin extraction of C3H/10T1/2 cell treated with BP, Dx or BP/Dx yielded two components, DNA-S and DNA-P. In case of BP treated, the topoisomerase activity was exist in both fraction but the group with Dx was activity in DNA-S only when the enzyme activity was determined in the presence of Dx, it was only higher than that of BP in DNA-S fraction. 5. The Western blot immunodetection of the above fractions indicated that DNA topoisomerase II existed in DNA-S and DNA-P fractions for the BP treated group but Dx trated group was in DNA-S fraction only. However, the amount of enzyme in the DNA-S of BP/Dx treated group were increased to compare with Dx treated group. These results indicated that the high salt insoluble fraction was representative region to topoisomerase II activity, also the DNA-S fraction by the action of Dx may play an important role in the function of a gene.