Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

프리캐스트 포스트텐션 콘크리트 넓은 보-기둥 내부 접합부의 비탄성 거동

황재환,최윤철,김영찬,김대중,임재형,권기혁,문정호,이리형 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

The purpose of this paper is to evaluate the response of Precast Prestressed Concrete Interior Wide Beam-Column Joint subjected to cyclic lateral loading. To this end, the experimental investigation was performed with six half-scale specimens of interior connection. The design parameters are the beam to column depth ratio, and the number of tendons within column core. One of the specimens, IJH-6, is modeled for the connection at the top floor of a structure. The test results showed that the specimens sufficiently resist up to limiting drift ratio of 0.035 in accordance with the provisional standard by ACI of acceptance criteria for concrete special moment frames.

아리스톨로크산 함유 생약제에 대한 안전성평가연구 : 3개월간 반복투여독성시험을 통한 신장독성평가

황명실,박미선,문지영,이지선,염영나,이효민,신동환,강진석,윤은경,최미나,육미영,장동덕,길광섭,김승희,양기화 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 실험에서는 아리스톨로크산을 함운하고 있는 생약제중 하나인 마두령(.4risfoforfioe JTurruf)의 90일 반복투여독성시헌을 실f,」하였다. 마두령에서 아리스톨로크산을 정량분석한 결과 마두령 단일 건조븐말의 경운 2.112mg/g, 잉상에서 처방되고 있는 마든령복방 (마두령외 9가지 생약제 복합처방) 건조분말꼭 경무에는 0.066m9/5으로 각각 검출되었다. 본 실험에서 용량설정은 마두령 처리군 (저용량 군; 21.3m9/k9/day (임상용량), 중용량군; 2t3mgfg/day, 고용량군; 2430mg딘g b.w./day), 마두령복방 처리군 (427mg/kg b.w./da?; 임상용량) 및 positive control (아리스톨로크산) 처리군 (저용량군;0.05mgag/da17, 중용량군; 0.Smg/kg/day, 고용량군; 5mg./kg/dal·1으료 하여 랫드에게 경구투여하였다. 마두령단방 처궈근 및 마두령복방 처리군에서 저용량군과 중응량군에서는 잉상관찰 및 생화학적 분석에서 패조군과 유의적인 차이가 없었다. 그러나 마두령단방 고용량근에서는 간, 신장의 상대적인 무계가 증가되었고, 신장의 유두이행상피증식 및 암종이 관찰되었으며, 위에서는 편평세포암종이 관찰되었다. 하자만 간손상이나 간암은 유발하지는 않는 것으로 판단되었다. 결론끌으로 본 실험의 결과에서는 아리스톨로크산 함유 생약제인 마두령이 임상용량인 저응량 처리군에서는 독성을 나타내지 않았으나, 고용쏭으로 장기 복용시켰을 깅우 설치류의 전위부위 및 신장에서 독성이 있음을 알 수 있었다. Chines herbs nephropathy (CHN) has been described in young women who had taken a slimming pills containing some chines herbs. Aristolochic acid (AA), suspected substance as the causal factor of CHN, is known a carcinogen. The Aristolochiae fructus (fruit of Aristolochia contorta) was used in Korean Traditional Medicine consists of appropriate amounts of mixed natural products. Subchronic toxicity of A. furctus containing aristolochic acid was investigated in SD rats. The body weight and clinical signs were observed after orally administration of A. futctus at doses of 21.2, 213, and 2130 mg/kg/day; mixture (including 9 other herbs) at dose of 427 mg/kg/day; aristolochic aicd (Ⅰ+Ⅱ) at doses of 0.05, 0.5, and 5 mg/kg/day for 3 months. At the end of the treatment, high dose treated animals revealed a deficit in final body weight about 25% compared with that of control. Organ weights of kidney, liver, testis, or ovary were increased with dose dependent manner. No changes considered to be due to the administration of A. furctus, mixture or aristolochic acid were in hematological and clinical study. However, significant changes at histopathological study of kidney and stomach were boserved in high-dose treated groups. In conclusion, for human health safety it needs appropriate regulatory actions regarding the use of natural herbal medicines known or suspected of containing aristolochic acid.

국채의 현·선물수익률간의 선후행 관계 분석

이기환,황두건 한국해양대학교 국제해양연구소 2001 韓國海洋大學校 人文社會科學論叢 Vol.13 No.1

Abstract This paper examines the lead-lag relationship between spot and futures prices of Korea Treasury Bond (KTB) with using daily return data from 29 September 1998 to 18 August 2000. Five kinds of futures are listed on Korea Futures Exchange (KOFEX) since the launching of April 1999. KTB futures grew as an important futures since the listing on KOFEX on 28th September 1999 and now the trade volume of KTB futures is the most among the listed futures. Bank, investment trust, insurance companies and foreigners are major investors of KTB futures. KTB's main financial function is to provide risk management system for mid-term interest rate instrument and also to discover its price in the future. In particular, this study focuses on the function of price discovery of futures over the expected spot price. The price of KTB futures reflects the future interest level which financial institutions or company experts could predict. Therefore, KTB futures could be seen as suggesting proper price for spot goods market and also it has function of discovering future value of KTB. There is time difference between spot and futures prices of KTB. We utilize unit root test, cointegration test, vector error correction models in order to analyze the relationship between spot and futures returns. The main finding of this study is that the futures price of KTB leads the spot price of KTB. That is, the futures price leads two days over the spot price. Moreover, two market's lead-lag relationship could be influenced by the trading volume of KTB futures. Then, an empirical analysis of futures prices of June and September 2000 exhibits that the spot price leads the futures price. Therefore, it is argued that there is a feedback relationship between spot and futures of KTB and the infrequent trading has an impact on the relationship between spot and futures prices. Even though the relationship between spot and futures prices is not stable, our finding implies that the discovery function of futures over the expected spot price is working in the Korea Futures Exchange. In particular, the period of our study is too short to investigate in depth the lead-lag relationship between spot and futures prices. Because of this limitation, we could not examine the difference of two markets's microstructure such as price volatility, trade pattern and so on. Therefore, it is thought that we could not find correct reasons about the lead-lag relationship of two markets. With the passage of KOFEX history, the trading volume and the efficiency of information will be increased with the growth of participants of this market and then we could explore the lead-lag relationship between spot and futures prices of KTB.

방사선 조사선량에 따른 백서 악하선의 caspase-3 발현양상

권기정,최용석,황의환,이상래,고광준 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To investigate the caspase-3 expression in the acinar and ductal cells of rat submandibular glands after the irradiation of various doses. Materials and Methods: The male Sprague-Dawley rats weighing approximately 250 gm were used for this study. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. The rats were sacrificed on the 1st, 3rd, 7th, 14th, 21st, and 28th day after irradiation. The specimens including the submandibular gland were sectioned and observed using histopathological and immunohistochemical methods. Results : The local destruction of the acinar and ductal cells and the karyopyknotic nuclei of the acinar cells were observed in the 2 Gy and 5 Gy irradiation groups later than in the 10 Gy and 15 Gy irradiation groups. And the expression of caspase-3 was prominent only in the ductal cells in the 2 Gy and 5 Gy irradiation groups. Conclusion : This experiment suggests that radiation-induced apoptosis in the ductal cells of rat submandibular glands was induced by a low dose radiation associated with the activation of caspase-3 and radiation-induced necrosis was induced by a high dose radiation. (Korean J Oral Maxillofac Radiol 2006; 36 : 7-15)

방사선조사가 당뇨 백서의 측두하악관절에 미치는 영향

안기동,황의환,이상래 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.2

Purpose : To investigate the histopathological changes in the temporomandibular joint in streptozotocin-induced diabetic rat following irradiation. Materials and Methods : Sprague-Dawley rats weighing about 250 gm were divided into three groups: control, diabetic, and diabetic-irradiated groups. Diabetes mellitus was induced in the rats by injecting streptozotocin. Rats in the control group were injected with citrate buffer only. After 5 days, the head and neck region of the rats in diabetic- irradiated group were irradiated with single absorbed dose of 10 Gy. The rats were killed at 1, 3, 7, 14, 21, and 28 days after irradiation. The specimen including the temporomandibular joint were sectioned and observed using a histopathological method. Results : In the diabetic group, severe bone resorption in the mandibular condyle was observed throughout the period of experiment. Necrosis of bone marrow and trabeculae was observed at 28 days after diabetic state. Atrophy and fibrosis in the retrodiscal tissue was gradually progressed during the time of the experiment. In the diabeticirradiated group, severe bone resorption in the mandibular condyle was observed during the early experimental phases, but regeneration of bone marrow was initiated at 14 days after diabetic state and irradiation. Also, calcification of abnormal trabeculae was observed at 28 days after diabetic state and irradiation. The retrodiscal tissue was degenerated in the early experimental phases, but it had been gradually regenerated during the experimental time. Conclusion : This experiment suggests that bone resorption and degeneration in the mandibular condyle are caused by the induction of diabetes, and abnormal bone formation is induced after irradiation in diabetic state.

방사선조사가 Streptococcus mutans에 미치는 영향

안기동,김규태,최용석,황의환 대한구강악안면방사선학회 2007 Imaging Science in Dentistry Vol.36 No.1

Purpose : To observe direct effect of irradiation on cariogenic Streptooccus mutans. Materials and Methods : S. mutans GS5 was exposed to irradiation with a single absorbed dose of 10, 20, 30, and 40 Gy. Viability and changes in antibiotic sensitivity, morphology, transcription of virulence factors, and protein profile of bacterium after irradiation were examined by pour plate, disc diffusion method, transmission electron microscopy, RT-PCR, and SDS-PAGE, respectively. Results : After irradiation with 10 and 20 Gy, viability of S. mutans was reduced. Further increase in irradiation dose, however, did not affect the viability of the remaining cells of S. mutans. Irradiated S. mutans was found to have become sensitive to antibiotics. In particular, the bacterium irradiated with 40 Gy increased its susceptibility to cefotaxime, penicillin, and tetracycline. Under the transmission electron microscope, number of morphologically abnormal cells was increased as the irradiation dose was increased. S. mutans irradiated with 10 Gy revealed a change in the cell wall and cell membrane. As irradiation dose was increased, a higher number of cells showed thickened cell wall and cell membrane and lysis, and appearance of ghost cells was noticeable. In RT-PCR, no difference was detected in expression of gtfB and spaP between cells with and without irradiation of 40 Gy. In SDS-PAGE, proteins with higher molecular masses were gradually diminished as irradiation dose was increased. Conclusion : These results suggest that irradiation affects the cell integrity of S. mutans, as observed by SDS-PAGE, and as manifested by the change in cell morphology, antibiotic sensitivity, and eventually viability of the bacterium. (Korean J Oral Maxillofac Radiol 2007; 37: 35-43)

디지탈방사선촬영술을 이용한 인접면 치아우식증 진단에 관한 실험적 연구

권기성,황의환,이상래 慶熙大學校 齒科大學 1994 慶熙齒大論文集 Vol.16 No.1

The purposes of this study were clinical comparison and evaluation between digital radiography and conventional radiography for the detection of artificial interproximal caries. Four freshly extracted, unrestored posterior teeth were obtained and canes was simulated by drilling semicircled defect with variable size at the interproximal surface of each tooth. The experiments were performed with IBM-PC/32 bit-DX compatible, video camera(VM-S8200, Hitachi Co., Japan), color monitor(Multisync 3D, NEC, Japan). Sylvia Image Capture Board for the ADC(analog to digital converter) was used, and spatial resolution was 512 x 480 with 256 gray levels. The obtained results were as follows: 1. At the condition of under exposure, the radiographs were superior to the digital images in readability. Also, as the size of the artificial lesion was increased, readability of the radiographs was elevated. 2. The digital images were superior to the radiographs in readability especially under over exsposure. 3. As the exposure time and size of lesion was increased, the gray level of region of interest of the digital image was decreased proportionally. 4. As the F-value of average gray level of region of interest at individual exposure time and size of lesion, gray level of the all lesion sizes was decreased in comparison with that of the normal enamel.

급성 포름알데히드 가스 흡입에 의한 흰쥐 후각상피의 형태학적 변화

백선용,전재기,문용석,황승영,노환중,고의경,왕수건 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.3

PVC 및 Olefin계 Copolymer의 열안정성에 관한 연구(II) : 열안정 영향 인자 Thermal Stability Factor

송해영,황택성,맹기석,박인환 忠南大學校 産業技術硏究所 1986 산업기술연구논문집 Vol.1 No.2

Several structural factors which governs the thermal stability of poly(vinyl chloride) were investigated by IR and H¹-NMR spectroscopy. PVC-Olefin copolymers having diverse structure were prepared by same reaction conditions such as reaction temperature and comonomer composition. It was found that keto allyl groups in the polymer backbone which were formed through a side reaction show the most pronauncing effect. The content of keto allyl group appeared to increase with the relative amount of olefin comonomers. On the other hand, neither chain branching nor tecticity influenced the thermal stability of copolymer. Also, the content of double bond fromed at chain ends was unrelated to the thermal stability.