김해시 인력ㆍ기구의 적정규모와 효율적 조직체계의 구축

김행범,강성철,이광수 인제대학교 김해발전연구소 1997 김해발전연구 Vol.1 No.1

간호사의 임상 실무 경험

서문자,손행미,강현숙,권성복,김주현,박영숙,이은희,임난영,조경숙,지성애 성인간호학회 2002 성인간호학회지 Vol.14 No.4

purpose: This study was conducted to describe qualitatively the entities of nurse's experiences in general hospitals and to suggest basic data guiding research on developing Standards of clinical nursing practice in Korea. Method: Fourteen nurses working at general hospitals with over 300 beds in Seoul were interviewed in-depth until saturation using tape-recorders and transcription. Result: The central theme of clinical nursing practice experienced by subjects was "being with clients" that means accepting client's personal character, solving client's needs and providing client-centered nursing. A also "being with clients" was felt to be the responsibility of nurses which was learned from their nursing schools. The nursing strategies performed in order to be with patients were proving skillful nursing techniques, accepting, educating, emotional support, advocating, and self-reflecting, the subjects experienced somewhat problematic affects such as difficulties in interpersonal relationship, work overload, negative image of nursing, deficit of self-confidence for nursing actions, poor working conditions, and unfair treatment. Nurses at the hospital practiced with pride when they felt that they were accepted by clients. Conclusion: Further research is needed to analysis problems in clinical practice and the comparison of nurses' experiences of clinical practice, with nurses' experiences in various settings.

대혈관 전위증의 병리

조수연,오미혜,김정선,이용희,강행지,서정욱 한국소아심장연구회 2003 소아심장 Vol.7 No.2

수출입 컨테이너 장치장 배정을 위한 소프트웨어의 개발

김갑환,김홍배,홍봉희,김기영,배종욱,최진오,김두열,이영기,박영만,박강태,손행대 한국경영과학회 1995 經營 科學 Vol.12 No.3

The Pusan Container Terminal faces a rapid increase in berthing time of container ships as well as in waiting time of external trucks, which is due to an absolute lack of yard space. This research is focused on the development of a decision support system for the planning of the container terminal yard assignment so that the yard space would be utilized most effectively. Efforts should be given to the reasonable assignment of the yard storage and the dynamic adaptation to the ever changing environment. The software introduced here is based o the know-how of the field exports and its framework takes the approach of the hierarchical decision making.

Quercetin, the active phenolic component in kiwifruit, prevents hydrogen peroxide-induced inhibition of gap-junction intercellular communication

Lee, Dong Eun,Shin, Bong Jik,Hur, Haeng Jeon,Kim, Jong Hun,Kim, Jiyoung,Kang, Nam Joo,Kim, Dae Ok,Lee, Chang Yong,Lee, Ki Won,Lee, Hyong Joo Cambridge University Press 2010 The British journal of nutrition Vol.104 No.2

<P>We evaluated the effects of the two main kiwifruit cultivars (gold kiwifruit (GOK) and green kiwifruit (GRK)) and their active phenolic compound, quercetin, on H2O2-induced inhibition of gap-junction intercellular communication (GJIC) in WB-F344 rat liver epithelial cells. We found that both GOK and GRK protect WB-F344 cells from H2O2-induced inhibition of GJIC. The extracellular signal-regulated protein kinase 1/2 (ERK1/2)-connexin 43 (Cx43) signalling pathway is crucial for the regulation of GJIC, and both GOK and GRK blocked the H2O2-induced phosphorylation of Cx43 and ERK1/2 in WB-F344 cells. Quercetin alone attenuated the H2O2-mediated ERK1/2-Cx43 signalling pathway and consequently reversed H2O2-mediated inhibition of GJIC in WB-F344 cells. A free radical-scavenging assay using 1,1-diphenyl-2-picrylhydrazyl showed that the scavenging activity of quercetin was higher than that of a synthetic antioxidant, butylated hydroxytoluene, per mol, suggesting that the chemopreventive effect of quercetin on H2O2-mediated inhibition of ERK1/2-Cx43 signalling and GJIC may be mediated through its free radical-scavenging activity. Since the carcinogenicity of reactive oxygen species such as H2O2 is attributable to the inhibition of GJIC, GOK, GRK and quercetin may have chemopreventive potential by preventing the inhibition of GJIC.</P>

[S13-3] Development of Korea Food and Nutrient Data System

Haeng Shin Lee,Young Ai Jang,Yoonna Lee,Hae Jeung Lee,Hye-Kyung Park,Yun-Sook Kang,Hye-Young Lee,Youn-Ju Choi,Cho-il Kim 한국식품영양과학회 2005 한국식품영양과학회 학술대회발표집 Vol.2005 No.10

Suppression of the <i>β-carotene hydroxylase</i> gene increases β-carotene content and tolerance to abiotic stress in transgenic sweetpotato plants

Kang, Le,Ji, Chang Yoon,Kim, Sun Ha,Ke, Qingbo,Park, Sung-Chul,Kim, Ho Soo,Lee, Hyeong-Un,Lee, Joon Seol,Park, Woo Sung,Ahn, Mi-Jeong,Lee, Haeng-Soon,Deng, Xiping,Kwak, Sang-Soo Elsevier 2017 Vol. No.

<P><B>Abstract</B></P> <P>β-carotene, a carotenoid that plays a key photo-protective role in plants is converted into zeaxanthin by β-carotene hydroxylase (CHY-β). Previous work showed that down-regulation of <I>IbCHY-β</I> by RNA interference (RNAi) results in higher levels of β-carotene and total carotenoids, as well as salt stress tolerance, in cultured transgenic sweetpotato cells. In this study, we introduced the RNAi-<I>IbCHY-β</I> construct into a white-fleshed sweetpotato cultivar (cv. Yulmi) by <I>Agrobacterium</I>-mediated transformation. Among the 13 resultant transgenic sweetpotato plants (referred to as RC plants), three lines were selected for further characterization on the basis of <I>IbCHY-β</I> transcript levels. The RC plants had orange flesh, total carotenoid and β-carotene contents in storage roots were 2-fold and 16-fold higher, respectively, than those of non-transgenic (NT) plants. Unlike storage roots, total carotenoid and β-carotene levels in the leaves of RC plants were slightly increased compared to NT plants. The leaves of RC plants also exhibited tolerance to methyl viologen (MV)-mediated oxidative stress, which was associated with higher 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical-scavenging activity. In addition, RC plants maintained higher levels of chlorophyll and higher photosystem II efficiency than NT plants after 250 mM NaCl stress. Yield of storage roots did not differ significantly between RC and NT plants. These observations suggest that RC plants might be useful as a nutritious and environmental stress-tolerant crop on marginal lands around the world.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Transgenic sweet potato plants were generated by RNAi silencing of the <I>IbCHY-β</I> gene. </LI> <LI> Transgenic sweet potato plants had increased β-carotene and total carotenoid content. </LI> <LI> Transgenic sweet potato plants exhibited strong antioxidant capacity and salt stress tolerance. </LI> </UL> </P>

Endoplasmic reticulum stress activates transglutaminase 2 leading to protein aggregation

LEE, JIN-HAENG,JEONG, JAEHO,JEONG, EUI MAN,CHO, SUNG-YUP,KANG, JEONG WOOK,LIM, JISUN,HEO, JINBEOM,KANG, HYUNSOOK,KIM, IN-GYU,SHIN, DONG-MYUNG UNKNOWN 2014 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.33 No.4

<P>Aberrant activation of transglutaminase 2 (TGase2) contributes to a variety of protein conformational disorders such as neurodegenerative diseases and age-related cataracts. The accumulation of improperly folded proteins in the endoplasmic reticulum (ER) triggers the unfolded protein response (UPR), which promotes either repair or degradation of the damaged proteins. Inadequate UPR results in protein aggregation that may contribute to the development of age-related degenerative diseases. TGase2 is a calcium-dependent enzyme that irreversibly modifies proteins by forming cross-linked protein aggregates. Intracellular TGase2 is activated by oxidative stress which generates large quantities of unfolded proteins. However, the relationship between TGase2 activity and UPR has not yet been established. In the present study, we demonstrated that ER stress activated TGase2 in various cell types. TGase2 activation was dependent on the ER stress-induced increase in the intracellular calcium ion concentration but not on the TGase2 protein expression level. Enzyme substrate analysis revealed that TGase2-mediated protein modification promoted protein aggregation concurrently with decreasing water solubility. Moreover, treatment with KCC009, a TGase2 inhibitor, abrogated ER stress-induced TGase2 activation and subsequent protein aggregation. However, TGase2 activation had no effect on ER stress-induced cell death. These results demonstrate that the accumulation of misfolded proteins activates TGase2, which further accelerates the formation of protein aggregates. Therefore, we suggest that inhibition of TGase2 may be a novel strategy by which to prevent the protein aggregation in age-related degenerative diseases.</P>

Possible association between G-protein β3 subunit C825T polymorphism and antipsychotic-induced restless legs syndrome in schizophrenia

Kang, Seung-Gul,Lee, Heon-Jeong,Choi, Jung-Eun,Park, Jae-Hong,Lee, Sang-Shin,Han, Changsu,Kim, Yong-Ku,Kim, Seung-Hyun,Lee, Min-Soo,Joe, Sook-Haeng,Jung, In-Kwa,Kim, Leen Blackwell Publishing Ltd 2007 Acta neuropsychiatrica Vol.19 No.6

<P>Objective</P><P>The incidence of restless legs syndrome (RLS) is presumed to be higher among people with schizophrenia who take antipsychotic medication, most of which blocks the dopamine D2 receptor. The purpose of this study was to determine whether the G-protein β<SUB>3</SUB> subunit (<I>GNB3</I>) C825T polymorphism is associated with antipsychotic-induced RLS in schizophrenia.</P><P>Methods</P><P>We examined 178 Korean patients with schizophrenia. All of the subjects were evaluated using the diagnostic criteria of the International Restless Legs Syndrome Study Group and the International Restless Legs Scale. Genotyping was performed for the C825T polymorphism in the <I>GNB3 </I>gene.</P><P>Results</P><P>The genotype distribution did not differ significantly between antipsychotic-induced RLS patients and patients who had no-RLS symptoms (<I>χ</I><SUP>2</SUP> = 4.30, <I>p </I>= 0.116). The genotypes of the C825T single-nucleotide polymorphism (SNP) were classified into two groups: C+ (CC and CT genotypes) and C– (TT genotype). The presence of the C allele (C+) was associated with an increased likelihood of RLS (<I>χ</I><SUP>2</SUP> = 4.14, <I>p </I>= 0.042; odds ratio = 2.56, 95% confidence interval = 1.02–6.47).</P><P>Conclusions</P><P>These results suggest that the GNB3 C825T SNP is associated with RLS in schizophrenia. However, confirming this association requires future larger scale studies in which the effects of medication are strictly controlled.</P>

Adiponectin is a potential catabolic mediator in osteoarthritis cartilage

Kang , Eun Ha,Lee, Yun Jong,Kim, Tae Kyun,Chang, Chong Bum,Chung, Jin-Haeng,Shin, Kichul,Lee, Eun Young,Lee, Eun Bong,Song, Yeong Wook BioMed Central 2010 ARTHRITIS RESEARCH AND THERAPY Vol.12 No.6

<P><B>Introduction</B></P><P>Adiponectin has been implicated in the pathogenesis of osteoarthritis (OA). We studied the effects of adiponectin on the OA cartilage homeostasis.</P><P><B>Methods</B></P><P>Immunohistochemical analysis was performed to evaluate differential expression of adiponectin receptors (AdipoRs) in nonlesional and lesional areas of OA cartilage. Cartilage and chondrocytes from the knee joints of primary OA patients were cultured in the presence of adiponectin (0~30 μg/ml). The levels of total nitric oxide (NO), matrix metalloproteinase (MMP)-1, -3, and -13, and tissue inhibitor of metalloproteinase (TIMP)-1 were measured in the conditioned media. The levels of inducible NO synthase (iNOS) and MMPs were determined with the quantitative real-time reverse transcription-polymerase chain reaction. The concentrations of collagenase-cleaved type II collagen neoepitope (C1-2C) were determined in the supernatant of adiponectin-stimulated OA cartilage explants. The effects of kinase and NOS inhibitors were evaluated in the adiponectin-stimulated chondrocytes.</P><P><B>Results</B></P><P>The expression levels of both AdipoR1 and AdipoR2 were significantly higher in lesional than in nonlesional areas of OA cartilage. The increased rate of AdipoR1-positive chondrocytes was twice that of AdipoR2-positive chondrocytes when compared between nonlesional and lesional areas. Adiponectin-stimulated OA chondrocytes showed increased total NO and MMP-1, -3, and -13 levels compared with nonstimulated cells. The TIMP-1 level was not affected. The C1-2C levels were increased by adiponectin in OA cartilage explant culture. AMP-activated protein kinase (AMPK) and c-Jun N-terminal kinase (JNK) inhibitors (compound C and SP600125) significantly suppressed adiponectin-induced production of total NO and MMP-1, -3, and -13. Inducible NOS inhibitors enhanced the expression of the adiponectin-induced MMPs.</P><P><B>Conclusions</B></P><P>Adiponectin causes matrix degradation in OA cartilage and increases MMPs and iNOS expression via the AMPK and JNK pathways in human OA chondrocytes. The catabolic effects of adiponectin may be counteracted by NO.</P>