The enhanced IL-l8 production by UVB irradiation requires ROI and AP-1 signaling in human keratinocyte cell line (HaCaT)

Cho, Daeho,Kang, Jae Seung,Park, Jong Hoon,Kim, Young-In,Hahm, Eunsil,Lee, Junechul,Yang, Yoolhee,Jeon, Junho,Song, HyunKeun,Park, Hyunjeong,Kim, Taesung,Pang, Saic,Kim, Chul-Woo,Hwang, Young Il,Lee, 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-

Based on our recent observation that enhanced IL-18 expression positively correlates with malignant skin tumors, such as SCC and melanoma, we examined the possible role of UVB, known to be associated with skin cancer development, in the enhancement of IL-18 production using primary human epidermal keratinocytes and human cell line HaCaT. After cells were exposed to UVB irradiation in vitro, IL-18 production was examined by Northern blot analysis and ELISA, and it was found that IL-18 production is enhanced by UVB irradiation in a dose- and time-dependent manner. In addition, we confirmed that it is functionally active form of IL-18 using the inhibitor of caspase-1. The effect of UVB irradiation was blocked by antioxidant, N-acetyl-ι-cysteine (NAC), which suggested the involvement of reactive oxygen intermediates (ROI) in the signal transduction of UVB irradiation-enhanced IL-18 synthesis. We also found that UVB irradiation increased AP-1 binding activity by using EMSA with AP-1-specific oligonucleotide. Furthermore, inhibitors of UVB-induced AP-1 activity, such as PD98059, blocked enhanced IL-18 production, indicating that AP-1 activation is required for UVB-induced IL-18 production. Taken together, our results suggest that UVB irradiation-enhanced IL-18 production is selectively mediated through the generation of ROI and the activation of AP-1.

Regulation of Proinflammatory Mediators via NF- <i><i>κ</i></i> B and p38 MAPK-Dependent Mechanisms in RAW 264.7 Macrophages by Polyphenol Components Isolated from Korea <i>Lonicera japonica THUNB</i>

Park, Kwang-Il,Kang, Sang-Rim,Park, Hyeon-Soo,Lee, Do Hoon,Nagappan, Arulkumar,Kim, Jin A,Shin, Sung Chul,Kim, Eun Hee,Lee, Won Sup,Chung, Hyon-Jong,An, Su Jin,Kim, Gon Sup Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-

<P><I>Lonicera japonica THUNB.</I>, which abundantly contains polyphenols, has been used as a traditional medicine for thousands of years in East Asian countries because of the anti-inflammation properties. This study aimed to investigate the anti-inflammatory mechanism of polyphenol components isolated from Korea <I>L. japonica T.</I> by nuclear factor-kappaB (NF-<I><I>κ</I></I>B) and mitogen-activated protein kinases (MAPKs) pathway. Polyphenols significantly decreased lipopolysaccharide- (LPS-) induced mRNA and protein expression of inducible nitric oxide synthase and cyclooxygenase-2, as well as mRNA expression of tumor necrosis factor-alpha, interleukin- (IL-) 1<I><I>β</I></I>, and IL-6. Moreover, polyphenols inhibited nuclear translocation of NF-<I><I>κ</I></I>B p65, phosphorylation/degradation of the inhibitor of <I><I>κ</I></I>B, and phosphorylation of p38 MAPK, whereas the extracellular signal-regulated kinase and Janus N-terminal kinase were not affected. These results indicate that polyphenol components isolated from Korea <I>L. japonica T.</I> should have anti-inflammatory effect on LPS-stimulated RAW 264.7 cells through the decrease of proinflammatory mediators expression by suppressing NF-<I><I>κ</I></I>B and p38 MAPK activity.</P>

Poster Session:PS 0239 ; Gastroenterology : Vitamin C Insuffi ciency Aggravates Dextran Sulfate Sodium (DSS)-Induced Colitis in Gulo(-/-) Mice

( Hye Min Kim ),( Jae Seung Kang ),( Jong Pil Im ),( Se Yeon Bae ),( Ye Jin Kim ),( Hang Rae Kim ),( Joo Sung Kim ),( Young Il Hwang ),( Wang Jae Lee ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Mucosal damage in inflammatory bowel diseases (IBDs) involves the dysfunctional immunoregulation of the gut. Among immunoregulatory factors, oxidative stress is abnormally high level in IBDs, and their destructive effects may contribute to the initiation or propagation of the disease. Vitamin C has both anti-oxidant and immunomodulatory effects. Methods: we investigated the effect of vitamin C on dextran sulfate sodium (DSS)-induced colitis in Gulo(-/-) mice which cannot synthesize vitamin C. Results: Vitamin C-insufficient Gulo(-/-) mice showed decreased survival with increased oxidative stress and more severe colitis. The production of interleukin (IL)-6 was higher, and STAT3 and Akt were more activated in DSS-treated vitamin C-insuffi cient Gulo(-/-) mice than in vitamin C-suffi cient Gulo(-/-) mice and wild-type mice. In contrast, the production of IL-22, the recruitment of NKp46(+) cells, and the activation of p38 MAPK were decreased in the vitamin C-insuffi cient Gulo(-/-) mice accompanied by decreased mucin-1 expression. Taken together, vitamin C insuffi ciency was associated with not only increased oxidative stress and IL-6 production but also decreased production of IL-22, which eventually induces severe colitis and death by DSS treatment. Conclusions: Therefore, our results suggest that vitamin C has a protective effect on DSS-induced colitis by regulating the production of cytokine and the induction of infl ammation.

큰느타리버섯 균사체로 제조한 발효두부 추출물의 면역 활성

이상원(Sang-Won Lee),강종우(Jong-Woo Kang),김재용(Jae-Yong Kim),박경욱(Kyung-Wuk Park),박석규(Seok-Kyu Park),주옥수(Ok-Soo Joo),이성태(Sung-Tae Yee),서권일(Kwon-Il Seo) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.1

두부의 기능성 및 저장성을 향상시킬 목적으로 큰느타리버섯 균사체를 이용한 발효두부를 제조하여 물과 메탄올로 추출하여 면역세포 활성에 미치는 효과를 조사하였다. 큰느타리버섯 균사체를 배양하기 위한 최적 배지는 PD broth 배지인 것을 확인하였으며, 큰타리버섯 균사체를 이용한 두부의 최적 발효기간은 7일 정도가 적당하였다. 큰느타리버섯 균사체를 이용하여 발효한 두부의 물 및 메탄올추출물은 0.01 ㎍/mL 농도 이상에서 비장세포의 증식을 유도하였으며, 이들 추출물은 IL-6, IFN-γ 분비를 유도하는 것으로 나타났다. 발효두부 물 추출물은 대조군에 비해 대식세포의 일산화질소 생산을 1 ㎍/mL 농도 이상에서 유의적으로 증가시키는 것을 알 수 있었으며, 메탄올 추출물은 10 ㎍/mL 농도 이상에서 그 생산을 증가시켰다. 발효두부 추출물들은 대식세포가 분비하는 IL-6, TNF-α, IL-1β 및 GM-CSF 분비량을 유의하게 증가시켰다. 따라서 큰느타리버섯 균사체로 발효한 두부는 기능성 두부로 개발이 가능하리라 생각된다. In order to improve the functional benefits and storage properties of soybean tofu, fermented tofu was developed using Pleurotus eryngii mycelia. The immune activities of water and methanol extracts of the tofu were investigated. The optimal medium for the growth of Pleurotus eryngii mycelia was PD broth medium and the optimal fermentation period for the tofu was 7 days. The water and methanol extracts of the fermented tofu induced the proliferation of spleen cells at above 0.01 ㎍/mL. The water extract increased IL-2, IFN-γ production, while the methanol extract increased IFN-γ synthesis. The water and methanol extracts of the fermented tofu induced the NO production in RAW264.7 macrophage cells at above 1 ㎍/mL and above 10 ㎍/mL concentration, respectively. The extracts also significantly increased the production of IL-6, TNF-α, IL-1β and GM-CSF in the cells. These results suggest that the tofu fermented with Pleurotus eryngii mycelia could be developed as a functional tofu.

<i>In vivo</i> genotoxicity evaluation of lung cells from Fischer 344 rats following 28 days of inhalation exposure to MWCNTs, plus 28 days and 90 days post-exposure

Kim, Jin Sik,Sung, Jae Hyuck,Choi, Byung Gil,Ryu, Hyeon Yeol,Song, Kyung Seuk,Shin, Jae Hoon,Lee, Jong Seong,Hwang, Joo Hwan,Lee, Ji Hyun,Lee, Gun Ho,Jeon, Kisoo,Ahn, Kang Ho,Yu, Il Je Informa Healthcare 2014 INHALATION TOXICOLOGY Vol. No.

<P>Despite their useful physico-chemical properties, carbon nanotubes (CNTs) continue to cause concern over occupational and human health due to their structural similarity to asbestos. Thus, to evaluate the toxic and genotoxic effect of multi-wall carbon nanotubes (MWCNTs) on lung cells <I>in vivo</I>, eight-week-old rats were divided into four groups (each group = 25 animals), a fresh air control (0 mg/m<SUP>3</SUP>), low (0.17 mg/m<SUP>3</SUP>), middle (0.49 mg/m<SUP>3</SUP>), and high (0.96 mg/m<SUP>3</SUP>) dose group, and exposed to MWCNTs <I>via</I> nose-only inhalation 6 h per day, 5 days per week for 28 days. The count median length and geometric standard deviation for the MWCNTs determined by TEM were 330.18 and 1.72 nm, respectively, and the MWCNT diameters ranged from 10 to 15 nm. Lung cells were isolated from five male and five female rats in each group on day 0, day 28 (only from males) and day 90 following the 28-day exposure. The total number of animals used was 15 male and 10 female rats for each concentration group. To determine the genotoxicity of the MWCNTs, a single cell gel electrophoresis assay (Comet assay) was conducted on the rat lung cells. As a result of the exposure, the olive tail moments were found to be significantly higher (<I>p</I> < 0.05) in the male and female rats from all the exposed groups when compared with the fresh air control. In addition, the high-dose exposed male and middle and high-dose exposed female rats retained DNA damage, even 90 days post-exposure (<I>p</I> < 0.05). To investigate the mode of genotoxicity, the intracellular reactive oxygen species (ROS) levels and inflammatory cytokine levels (TNF-α, TGF- β, IL-1, IL-2, IL-4, IL-5, IL-10, IL-12 and IFN-γ) were also measured. For the male rats, the H<SUB>2</SUB>O<SUB>2</SUB> levels were significantly higher in the middle (0 days post-exposure) and high- (0 days and 28 days post-exposure) dose groups (<I>p</I> < 0.05). Conversely, the female rats showed no changes in the H<SUB>2</SUB>O<SUB>2</SUB> levels. The inflammatory cytokine levels in the bronchoalveolar lavage (BAL) fluid did not show any statistically significant difference. Interestingly, the short-length MWCNTs deposited in the lung cells were persistent at 90 days post-exposure. Thus, exposing lung cells to MWCNTs with a short tube length may induce genotoxicity.</P>

돼지의 등지방층 두께 측정치와 일상증체량간의 관계

강민수,박영일,김종복 제주대학교 1984 논문집 Vol.17 No.-

The phenotypic correlations between backfat thickness and average daily gain were estimated in the basis of the data obtained from 167 boars of Duroc and Hampshire raised at the Je-il Farm over a 2-ycar period from January, 1981 to December 1982. The results obtained in this study are summarized as follows: 1. The phenotypic correlation between backfat thicness and average daily gain of Durocs was 0.173 for final weghts from 70 to 90kg, 0.854 for fmal weights from 91 to 100 kg, 0.190 for fmal weights from 101 to 101kg and 0.330 for fianl weights from 111 to 130kg. 2. In Hampshires, the phenotypic correlation between backfat thickness and average daily gain was 0.191, 0.827, 0.167 and 0.491 for final weights from 70 to 90kg, 91 to 100kg, 101 to 110kg, and 111 to 130kg, respectively. 3. The phenotypic correlation between backfat thickness and average daily gain pooled over two breeds was 0.384, 0.163, 0.211 and 0.433 for final weights from 70 to 90kg, 91 to 100kg, 101 to 110kg 111 to 130kg, respectively. 4. The phenotypic correlations between backfat thickness and average dialy gain of Duroes and Hamp-shirs were positive. The differences in the correlation coefficients between the two breeds or between the final weights were statistically insignificant. (p>0.5)

기관지천식 환자에서 Furosemide, Disodium cromoglycate 및 Heparin 흡입이 고장액 식염수 기관지유발검사에 미치는 영향

강천일,현상훈,남언정,김건우,윤종수,서영익,이종명,김능수 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.3

목적 : 기관지천식 환자에서 고장액 식염수의 흡입은 기도수축을 유발할 수 있으며 이는 운동유발성 천식반응과 밀접한 관계가 있는 것으로 알려져 있다. 저자들은 알레르겐 흡입이나 운동에 의해 유발되는 천식에 예방효과가 있는 것으로 알려진 disodium cromoglycate(DSCG), furosemide 및 heparin 흡입이 4.5% NaCl 기관지유발검사(BPT)에 미치는 영향을 조사하였다. 대상 및 방법 : 4.5% NaCl BPT에서 양성반응을 보이는 기관지천식 환자 13명을 대상으로 하였으며 사용된 약물은 furosemide 40㎎, DSCG 40㎎ 및 heparin 1,000μ/㎏이었다. 먼저baseline 4.5% NaCl BPT를 시행한 다음 이들 약물로 전처치후 다시 4.5% NaCl BPT를 시행하여 약물의 효과를 관찰하였다. 결과 : Furosemide 40㎎, DSCG 40㎎및 heparin 1,000μ/㎏의 흡입 전처치는 고장액 식염수에 의한 기도수축 반응에 뚜렷한 예방효과를 보였다. Furosemide와 DSCG로 전처치한 군(n=6)에서 이들의 기도수축 방어율은 각각 100.6±6.6%, 91.1±17.2%였으며 furosemide와 heparin으로 전처치한 군(n=7)에서는 각각 58.7±29.2%, 59.0±51.1%로서 각 군에서 이들 약제간의 방어율에는 유의한 차이가 없었다. 결론 : Furosemide(40㎎), DSCG(40㎎) 및 heparin(1000μ/㎏)의 흡입 전처치는 고장액 식염수에 의한 기도수축 반응에 뚜렷한 예방효과를 보였으며, 적어도 이 용량에서 기도수축 예방 정도에는 유의한 차이가 없음을 알 수 있었다. Background: Hypertonic saline (4.5% NaCl) inhalation is known to induce broncho-constriction by affecting mast cell, epithelial cell and vagal afferent pathway in some asthmatics. Disodium cromoglycate (DSCG) is known to have a preventive effect on allergic asthma and exercise induced asthma, and recently it was reported that furosemide and heparin had similar effect. The purpose of this study was to evaluate the effects of furosemide, DSCG and heparin on hypertonic saline provocation test in asthmatics. Methods: Thirteen asthmatics with a positive response to hypertonic saline challenge were enrolled. Hypertonic saline and test drugs were generated by ultrasonic nebulizer. After taking baseline 4.5% NaCl challenge, subjects were rechallenged with 4.5% NaCl after inhalation of furosemide 40㎎, DSCG 40㎎ or heparin 1,000μ/㎏. Results: 1. There was a significant positive relationship between PC_20-methacholine and PTM-4.5% NaCl(r=0.5575, p = 0.024). 2. Furosemide, DSCG and heparin had no direct bronchodilating effects. 3. Premedication of furosemide and DSCG(n=6) showed significant protective effects on 4.5% NaCl induced broncho-constriction. The average protection rate were 100.6±6.6% and 91.1±17.2%, respectively. 4. Premedication of furosemide and heparin(n=7) showed significant protective effects on 4.5% NaCl induced broncho-constriction. The average protection rate were 58.7±29.2% and 59.0±51.1%. respectively. Conclusions: Furosemide(40㎎), DSCG(40㎎) and heparin(1.000μ/㎏) had significant protective effects on hypertonic saline induced broncho-constriction in asthmatics, and there were no significant differences in their potency of protection rate.

LINEAR RECURSIVE QUERY의 효율적 처리를 위한 algorithm에 관한 연구

강종규,진성일 충남대학교 자연과학연구소 1987 忠南科學硏究誌 Vol.14 No.1

First-order database is defined as a function - free first - order theory. First -order database consists of the extensional database (EDB) and the intensional database (IDB). Henschen and Naqvi(HN algorithm), Wavefront algorithm,σ-Wavefront algorithm and counting method were developed for the efficient processing of first - order database queries in case of Transitive Closure queries(TC) and Single Looping rule and Single Recursion point (SLSR). We could develop an algorithm, so called, level counting which optimized the HN algorithm using counting method and σ-Wavefront algorithm, so we could reduce the redundancies and give the correct answer and guarantee the termination.

미세채널 구조물 상부의 초정밀 연마 기술 연구

강정일,이윤호,안병운,윤종학 한국공작기계학회 2003 한국공작기계학회 추계학술대회논문집 Vol.2003 No.-

Micro-Channel ultra-precision polishing is a new technology used in magnetic field-assisted polishing. In this paper, an electromagnet or the jig of test system was designed and manufactured. A size of magnetic abrasive is used on 25~75μm and for the polish a micro-channel upper part. A surface of channel which is not even is manufactured using magnetic abrasive finishing at upper surface of micro-channel. As a result, the surface roughness rose by 80% after upper surface of micro- channel was polished up 8 minutes by polishing.

스위스의 영세중립 모델 : 남북통일에 적용가능성 모색

강종일 동아대학교 동아시아연구원 2005 동아시아 : 비교와 전망 Vol.4 No.-

한반도의 국내외적 정치 환경을 극복하는데 스위스의 영세중립 모델은 우리에게 여러가지 시사점을 준다. 본 논문은 남북통일을 위한 새로운 대안으로써 스위스의 영세중립 모델을 적용할 수 있는 가능성을 모색하는데 초점을 둔다. 오늘날 한반도의 정세는 마치 1세기 전과 유사한 주변강대국의 헤게모니 경쟁 장소로 전개될 조짐을 보이고 있다. 미국은 일방주의 유일 강대국으로 북한의 핵문제를 평화적 협상보다는 무력에 의한 강압적 해결방법을 고집하고 있다. 중국은 동북공정계획을 통해 고구려 역사를 자국역사에 편입시키려는 문화적 패권주의를 지향하고 있다. 보통국가를 지향하고 있는 일본은 미-일동맹을 강화하면서 이라크 파병과 독도의 영유권에 대한 망언을 의도적으로 하고 있다. 러시아는 한반도 문제에 개입하기 위해 6자 회담에 참석하면서 시베리아 철도와 송유관 매설문제를 통해 북한에 영향력을 확대하고 있다. 한반도를 중심으로 한 강대국의 대립과 패권의 경쟁에서 남북은 주변국가의 국제정치 상황을 바르게 인식하고 갈등과 대립보다는 분단을 극복하려는 의지를 보여야 할 것이다. 더 나아가 남한은 통일의 환경 조성을 위해 보수와 진보, 지역과 지역, 부유층과 서민층,구세대와 신세대간의 대립과 갈등을 완화해야 할 것이다. 스위스는 수세기 동안 독일, 프랑스, 이탈리아 등 주변 국가로부터 무수한 외침과 식민지 경험을 했다. 스위스 국민은 독일계, 프랑스계, 이탈리아계의 다민족으로 구성되어 인종, 언어, 문화가 다양하기 때문에 과거 각 주들 간의 영토확장을 위한 전쟁과 민족간의 갈등이 계속되었다. 그러나 스위스는 중립정신으로 내전을 종식하고 1536년부터 외국의 침입을 방지하기 위해 영세중립 정책을 추구함으로써 1815년 비엔나(Vienna)회의에서 주변국가로부터 영세중립국의 국제적 지위를 부여받게 되었다. 그 후 스위스는 어떠한 국제전쟁에도 개입하거나 편의를 제공하지 않고, 외국으로부터 침입도 받지 않았기 때문에 오늘날 국민소득 1위의 모범국가로 성장했다. 한반도는 남북간의 이념 갈등을 극복하고 전쟁을 방지하면서 통일 후에는 주변국의 침입도 방지할 수 있는 제도적 장치가 필요하기 때문에 스위스의 영세중립 모델은 한반도 통일에 유용한 시사점을 준다. The purpose of this paper is to explore a possibility of applying a model based on Swiss permanent neutrality to the unification of the Korean Peninsula. Switzerland was in violent civil wars between 13 cantons in an effort to expand their territories from 1436 to 1467. Furthermore, it was continuously engaged in bloody battles throughout the 16th century; for example, Switzerland was heavily defeated by French aggressions in the Marignano war of 1515 and then again, in the Senalcaldian war of 1546. In 1815, Switzerland had two aims when it be came apermanent neutral state. One was to prevent internal civil wars between cantons, and the other was to protect itself from the foreign aggressions. The Korean Peninsula experienced a number of foreign aggressions as Switzerland had experienced in the 16th century. According to a statistics of the Korean history, there were about 720 foreign skirmishes including 53 foreign aggressive wars. In fact, a number of foreign invasion wars upon the Korean Peninsula was 33times from China, 17 times from Japan, once from each of three countries: France, the United States, and England. In this context, it is preferred that the Korean Peninsula is unified as a permanent neutral state in order to deter a possible war between South and North Korea, and the foreign aggression after Korean reunification. It is expected that there is a possibility for the Korean Peninsula to realize its permanent neutral unification based on the model of Swiss permanent neutrality.