낙동강 하류의 플랑크톤 군집 변이

최애란,손문호,김좌관,이진애 인제대학교 2007 仁濟論叢 Vol.22 No.1

Effects of Sweet Potato Fractions on Alcoholic Hangover and Gastric Ulcer

Jwa Jin Kim,Chang Whan Kim,Dongsun Park,Sunhee Shin,Jeong Hee Jeon,Min-Jung Jang,Hyeong-Jin Ji,Namgil O,Jugyeong Song,Jinsoo Lee,Byung-Yul Kim,Ehn-Kyoung Choi,Seong Soo Joo,Seock-Yeon Hwang,Yun-Bae Ki 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.2

Reddish purple-colored fruits and vegetables contain high level of anthocyanins, which are well known to exert antioxidant, cardioprotective and hepatoprotective actions. In the present study, the effects of sweet potato fermentation filtrate (SPF) and its fractions on alcoholic hangover and gastric ulcer were investigated. In order to evaluate antihangover effect, male Sprague-Dawley rats were orally administered with SPF (240 or 750 ㎎/㎏) 30 min prior to or 60 min after oral challenge with 3 mL/㎏ of ethyl alcohol (15 mL/㎏ of 20% in water), and the blood concentration of alcohol were analyzed up to 5 hours. Pretreatment with 240 and 750 mg/kg of SPF significantly reduced the blood ethanol concentration by 20-25% and 50-60%, respectively, which was superior to the effect of the Hovenia dulis Thunb extract. In addition, posttreatment of SPF enhanced the alcohol-clearance rate to 2.8 and 3.1 folds at doses of 240 and 750 ㎎/㎏, respectively. In comparison, liquid portion of SPF exerted similar antihanover effect to SPS, whereas the effect of purified anthocyanins was negligible, indicating that active ingredients are included in the liquid portion. For the assessment of antiulcer activity, fractions of SPF were orally treated 30 min before oral administration of absolute ethanol (3 mL/㎏) and intravenous injection of 1% Evan's blue (0.5 mL/㎏). One hour later, the ulcer index (㎜ of ulcer lesions) was measured, and dye leakage on the gastric wall was quantified by extracting the dye with formamide. SPF and its liquid portion (300 ㎎/㎏) near-fully prevented the ethanol-induced gastric ulcer and significantly reduced the Evan's blue leakage, while the purified anthocyanins were ineffective. Among subfractions, buthanol fraction of SPFliquid portion exerted a substantial antiulcer activity, which was superior to water and methanol fractions. Taken together, it was demonstrated that SPF possesses antihangover and antiulcer activities, and that buthanol fraction of SPF-liquid portion contains active ingredients. Therefore, it is suggested that SPF or its buthanol fraction could be a potential candidate for the attenuation of hangover and gastric ulcers induced by excessive drinking of alcohol, although the action mechanisms remain to be clarified.

Squamous odontogenic tumor:

Jwa-Young Kim,Jin-Cheol Kim,Byoung-Ouck Cho,Seong-Gon Kim,Byoung-Eun Yang,Horatiu Rataru 대한구강악안면외과학회 2007 대한구강악안면외과학회지 Vol.33 No.1

A squamous odontogenic tumor (SOT) is an epithelial originated benign tumor. It has been rarely reported and most was intramural type. We observed a case of SOT in the mandible. It was associated with the odontogenic cyst. It was shown positive to pancytokeratin and p53. Considering that the case was free from recurrence for 5 years after surgery, p53 positive did not seem to be related to the prognosis of the disease.

Poster Session : PS 1052; Gastroenterology (Gastrointestinal Tract) ; A Case of Esophago Mediastinal Fistula Due to Tuberculous Mediastinal Lymphadenitis

( Jwa Hoon Kim ),( Do Hoon Kim ),( Hee Young Yoon ),( Ji Wan Lee ),( Hyung Woo Cho ),( Soo Min Jeung ),( Eun Jeong Gong ),( Hwoon Yong Jung ),( Jin Ho Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Tuberculosis rarely involves the esophagus, and the most esophageal tuberculosis occurs secondary to adjacent tuberculous lymphadenitis. Esophago-mediastinal fi stula is very rare complication of tuberculous lymphadenitis. A 28-year-old, previously healthy female visited the outside hospital due to nausea, vomiting, and fever. Chest CT(Computed tomography) showed enlargement of multiple, mediastinal lymph nodes with air density in the mediastinum, and thus suggesting an esophago-mediastinal fi stula. Esophagogram showed the extraluminal contrast leakage in cervical esophagus. Esophagogastroduodenoscopy(EGD) showed the deep ulcer with the fi stula opening at 24cm from the upper incisor teeth. Tuberculosis(Tb) polymerase chain reaction(PCR) on the sputum was positive. A biopsy of the right subclavian lymph node revealed necrotizing granuloma with Tb PCR positive. We diagnosed as tuberculous mediastinal lymphadenitis with esophago-mediastinal fi stula. The referring doctor failed to close the fi stula by endoscopic clipping. Therefore, we decided to use anti-Tb medications to treat fi stula via gastrostomy, because of severe nausea and vomiting. After four weeks, EGD and esophagogram showed that the fi stula opening was closed. The present case highlights the importance of anti-Tb medications in treatment of esophago-mediastinal fi stula due to tuberculous mediastinal lymphadenitis. n.

Increased Neutrophil Extracellular Trap Formation in Uremia Is Associated with Chronic Inflammation and Prevalent Coronary Artery Disease

Kim, Jwa-Kyung,Hong, Chang-won,Park, Mi Jin,Song, Young Rim,Kim, Hyung Jik,Kim, Sung Gyun Hindawi 2017 Journal of immunology research Vol.2017 No.-

<P><I>Background.</I> Neutrophils are involved in the pathogenesis of atherosclerosis by neutrophil extracellular trap (NET) formation. We hypothesized that the NET formation of neutrophils might be changed in end-stage renal disease (ESRD) patients, explaining their higher incidence of coronary artery diseases (CAD). <I>Method.</I> A cross-sectional study was performed in 60 maintenance hemodialysis (MHD) patients, 30 age- and sex-matched healthy individuals (HV, negative control), and 30 patients with acute infection (positive control). Neutrophil activation and function were measured with reactive oxygen species (ROS) activity, degranulation, NET formation, and phenotypical changes. <I>Result.</I> Compared with HV, neutrophils extracted from MHD patients displayed significantly increased levels of basal NET formation, ROS production, and degranulation, suggesting spontaneous activation in uremia. Also, an increase in citrullinated histone H3 was detected in this group compared to the HV. And neutrophils from HV were normal CD16<SUP>bright</SUP>/CD62L<SUP>bright</SUP> cells; however, neutrophils from MHD were CD16<SUP>bright</SUP>/CD62L<SUP>dim</SUP>, similar to those from patients with acute infections. Interestingly, multivariate analyses identified the prevalent CAD and neutrophil counts as independent determinants of baseline NET formation (<I>β</I> = 0.323, <I>p</I> = 0.016 and <I>β</I> = 0.369, <I>p</I> = 0.006, resp.). <I>Conclusions.</I> Uremia-associated-increased NET formation may be a sign of increased burden of atherosclerosis.</P>

The relationship between autophagy, increased neutrophil extracellular traps formation and endothelial dysfunction in chronic kidney disease

Kim, Jwa-Kyung,Park, Mi Jin,Lee, Hoi Woul,Lee, Hyung Seok,Choi, Sun Ryoung,Song, Young Rim,Kim, Hyung Jik,Park, Hyeong-Cheon,Kim, Sung Gyun Elsevier 2018 Clinical immunology Vol.197 No.-

<P><B>Abstract</B></P> <P>In chronic kidney disease (CKD), the number of circulating neutrophils are increased, and this is usually accompanied by an increased basal activation state. However, the possible association between neutrophil extracellular traps (NETs) with vascular complications has not been evaluated. We assessed the relationship between NETs, autophagy and endothelial dysfunction in maintenance hemodialysis (MHD) patients. NET formation, neutrophil elastase (NE) activities, and serum nucleosome levels were measured in MHD (<I>n</I> = 60) and controls (<I>n</I> = 20). Basal NET formation were markedly increased in MHD patient compared to controls. After PMA stimulation, MHD neutrophils showed significantly increased NETs formation response than controls. The degree of NETs was strongly associated with lower flow-mediated dilatation(%) of brachial artery even after adjustment for cardiovascular risk factors and uremic toxins. Moreover, MHD neutrophils showed increased basal autophagy activity. Interestingly, the levels of NETs were markedly augmented after autophagy inhibition, suggesting a protective role of autophagy in excessive NET formation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In patients with CKD, NET formation is significantly increased, and it may directly contribute to endothelial dysfunction. </LI> <LI> Chronic uremia-associated increased autophagy activity could be an adaptive response. </LI> <LI> Blocking autophagy activities induced exaggerated NET release, suggesting a protective role of autophagy in CKD patients. </LI> </UL> </P>

Interleukin‐22 promotes osteoclastogenesis in rheumatoid arthritis through induction of RANKL in human synovial fibroblasts

Kim, Kyoung‐,Woon,Kim, Hae‐,Rim,Park, Jin‐,Young,Park, Jin‐,Sil,Oh, Hye‐,Jwa,Woo, Yun‐,Ju,Park, Mi‐,Kyung,Cho, Mi‐,La,Lee, Sang‐,Heon Wiley Subscription Services, Inc., A Wiley Company 2012 Vol.64 No.4

<P><B>Abstract</B></P><P><B>Objective</B></P><P>To examine the regulatory role of interleukin‐22 (IL‐22) in the expression of RANKL and induction of osteoclastogenesis in rheumatoid arthritis (RA).</P><P><B>Methods</B></P><P>Concentrations of IL‐22 and RANKL in the serum and synovial fluid of RA patients were measured using enzyme‐linked immunosorbent assay. RA synovial fibroblasts were treated with recombinant human IL‐22 (rhIL‐22), and the expression of RANKL messenger RNA (mRNA) and protein was measured using real‐time polymerase chain reaction, Western blotting, and intracellular immunostaining. Human monocytes were cocultured with IL‐22–prestimulated RA synovial fibroblasts and macrophage colony‐stimulating factor, and osteoclastogenesis was assessed by counting the multinucleated cells (those staining positive for tartrate‐resistant acid phosphatase).</P><P><B>Results</B></P><P>The IL‐22 concentration in the synovial fluid was higher in RA patients than in patients with osteoarthritis (OA). The serum IL‐22 concentration was also higher in RA patients than in OA patients and healthy volunteers, and this correlated with serum titers of rheumatoid factor and anti–cyclic citrullinated peptide antibodies. In RA synovial fibroblasts treated with rhIL‐22, the expression of RANKL mRNA and protein was increased in a dose‐dependent manner. IL‐22–induced RANKL expression was down‐regulated significantly by the inhibition of p38 MAPK/NF‐κB or JAK‐2/STAT‐3 signaling. In human monocytes cocultured with IL‐22–prestimulated RA synovial fibroblasts in the absence of exogenous RANKL, the monocytes differentiated into osteoclasts, but this osteoclastogenesis decreased after p38 MAPK/NF‐κB or JAK‐2/STAT‐3 signaling was inhibited.</P><P><B>Conclusion</B></P><P>These results show that IL‐22 up‐regulates RANKL expression in RA synovial fibroblasts and induces osteoclastogenesis. These effects are mediated by the p38 MAPK/NF‐κB and JAK‐2/STAT‐3 signaling pathways.</P>

Prognostic value of $^{18}F$-fluorodeoxyglucose positron emission tomography, computed tomography and magnetic resonance imaging in oral cavity squamous cell carcinoma with pathologically positive neck lymph node

Jwa, Eunjin,Lee, Sang-Wook,Kim, Jae-Seung,Park, Jin Hong,Kim, Su Ssan,Kim, Young Seok,Yoon, Sang Min,Song, Si Yeol,Kim, Jong Hoon,Choi, Eun Kyung,Ahn, Seung Do The Korean Society for Radiation Oncology 2012 Radiation Oncology Journal Vol.30 No.4

Purpose: To evaluate the prognostic value of preoperative neck lymph node (LN) assessment with $^{18}F$-fluorodeoxyglucose positron emission tomography ($^{18}F$-FDG PET), computed tomography (CT), and magnetic resonance imaging (MRI) in oral cavity squamous cell carcinoma (OSCC) patients with pathologically positive LN. Materials and Methods: In total, 47 OSCC patients with pathologically positive LN were retrospectively reviewed with preoperative $^{18}F$-FDG PET and CT/MRI. All patients underwent surgical resection, neck dissection and postoperative adjuvant radiotherapy and/or chemotherapy between March 2002 and October 2010. Histologic correlation was performed for findings of $^{18}F$-FDG PET and CT/MRI. Results: Thirty-six (76.6%) of 47 cases were correctly diagnosed with neck LN metastasis by $^{18}F$-FDG PET and 32 (68.1%) of 47 cases were correctly diagnosed by CT/MRI. Follow-up ranged from 20 to 114 months (median, 56 months). Clinically negative nodal status evaluated by $^{18}F$-FDG PET or CT/MRI revealed a trend toward better clinical outcomes in terms of overall survival, disease-free survival, local recurrence-free survival, regional nodal recurrence-free survival, and distant metastasis-free survival rates even though the trends were not statistically significant. However, there was no impact of neck node standardized uptake value ($SUV_{max}$) on clinical outcomes. Notably, $SUV_{max}$ showed significant correlation with tumor size in LN (p < 0.01, $R^2$ = 0.62). PET and CT/MRI status of LN also had significant correlation with the size of intranodal tumor deposit (p < 0.05, $R^2$ = 0.37 and p < 0.01, $R^2$ = 0.48, respectively). Conclusion: $^{18}F$-FDG PET and CT/MRI at the neck LNs might improve risk stratification in OSCC patients with pathologically positive neck LN in this study, even without significant prognostic value of $SUV_{max}$.

Highly photoluminescent N-isopropylacrylamide (NIPAAM) passivated carbon dots for multicolor bioimaging applications

Kim, Min Cheol,Yu, Kwang Sik,Han, Seung Yun,Kim, Jwa-Jin,Lee, Jae Won,Lee, Nam Seob,Jeong, Yeong Gil,Kim, Do Kyung Elsevier 2018 European polymer journal Vol.98 No.-

<P><B>Abstract</B></P> <P>We report a highly luminescent Carbon Dots passivated by nitrogen rich molecules, i.e. N-isopropylacrylamide (NIPAAM), with a QY as high as ca. 94%, which is the highest yield until now. NIPAMM@C-Dots are synthesized directly from the precursors composed of CA, EDA and NIPAAM by hydrothermal reaction. The optical properties of in situ synthesized NIPAAM@C-Dots showed EX-dependent EM at high concentration and EX-independent EM at low concentration. In most cations and anions except Fe<SUP>3+</SUP> and Bi<SUP>3+</SUP> added to the aqueous solution, NIPAAM@C-Dots exhibit an extremely stable inert behavior. In addition, in order to verify its usefulness as a fluorescent marker, C6 glioblastoma cells and NIPAAM@C-Dots were cultured together and then applied by both confocal bioimaging and flow cytometry analysis. The role of NIPAAM present on or around the C-Dots surface can improve QY values and stability, resulting in reduced cytotoxicity and can be a strong candidate as an alternative to organic dyes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Carbon Dots passivated by N-isopropylacrylamide (NIPAAM) with a QY of 94% are synthesized. </LI> <LI> NIPAAM@C-Dots showed EX-independent EM at low concentration. </LI> <LI> In most cations and anions except Fe<SUP>3+</SUP> and Bi<SUP>3+</SUP> added to the aqueous solution, NIPAAM@C-Dots exhibit an extremely stable inert behavior. </LI> <LI> C6 glioblastoma cells and NIPAAM@C-Dots were cultured together and then applied by both confocal bioimaging and flow cytometry analysis. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

S-516 A higher ultrafiltration rate is associated with worsening of the left atrial volume index

( Young Jin Ryu ),( Young Rim Song ),( Hyung Jik Kim ),( Sung Gyun Kim ),( Jwa-kyung Kim ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Background: Optimal fluid management is essential in caring hemodialysis patient. However, too rapid fluid removal and the resultant higher ultrafiltration rate (UFR) disadvantageously promote hemodynamic instability and cardiac injury. We evaluated the effects of the rapid UFR on the changes of echocardiographic left atrial volume index (LAVI) over period. Methods: A longitudinal observational study enrolled 124 new hemodialysis patients. Echocardiography was performed at baseline and repeated 19.7 (11.3-23.1) months apart. The changes in LAVI (?LAVI/yr, mL/m2.7/year) were calculated, and the 75th percentile of the ?LAVI/yr distribution was regarded as a “pathological” increment. The UFR was expressed in mL/h/kg, and we employed a mean UFR over 30 days (approximately 12~13 treatments). Results: The mean inter-dialytic weight gain was 1.88±0.94 kg, and the UFR were 8.01±3.87 mL/h/kg. The significant pathological increment point in ?LAVI/yr was 4.87 mL/1.73 m2/yr. Correlation analysis showed that ?LAVI/yr was closely related to the baseline blood pressure (BP), hemoglobin level, residual renal function, and UFR. According to the ROC curve, the best cut-off of value of UFR for the predicting the pathological increment was 10 mL/h/kg, with the area under the curve of 0.712. In multivariate analysis, systolic BP, a history of coronary artery disease, hemoglobin <10 g/dL, and high UFR were significant predictors. An increase of 1 mL/h/kg in the UFR was associated with a 22% higher risk of a worsening of the LAVI (odds ratio, 1.22; 95% confidence interval, 1.05-1.41). Conclusions: For patient starting hemodialysis, a rapid UFR over 10 mL/h/kg may be associated with maladaptive worsening of the LAVI, a strong predictor of long-term adverse outcomes. Keywords: ultrafiltration rate; left atrial volume index; mortality; hemodialysis; ESRD