Anti-hangover effects of a combinational preparation of yeast and rice embryo/soybean extracts

Jugyeong Song,Namgil O,Jae Myun Ryu,Tae Myoung Kim,Im Kwon Seo,Dongsun Park,Jeong Hee Jeon,Sunhee Shin,Seong Soo Joo,Yun-Bae Kim 한국실험동물학회 2008 한국실험동물학회 학술발표대회 논문집 Vol.2008 No.-

Inhibitory effect of red ginseng preparations on the growth of tumors xenografted in nude mice

Jinsoo Lee,Jugyeong Song,Dongsun Park,Jeong Hee Jeon,Sunhee Shin,Seong Soo Joo,Yun-Bae Kim 한국실험동물학회 2008 한국실험동물학회 학술발표대회 논문집 Vol.2008 No.-

Effects of a Preparation of Combined Glutathione-Enriched Yeast and Rice Embryo/Soybean Extracts on Ethanol Hangover

Heon-Sik Lee,Jugyeong Song,김태명,주성수,박동선,Jeong Hee Jeon,Sunhee Shin,Hyoung Kook Park,Won Kyung Lee,이선영,김미리,Do Ik Lee,김윤배 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.6

The effects of a preparation of combined glutathione-enriched yeast (GEY) and rice embryo/soybean (RES) extracts (20:1), GEY/RES, on experimentally induced ethanol hangover were investigated in male Sprague-Dawley rats. To evaluate the preventive effects on hangover, rats were orally administered GEY/RES (50/2.5, 100/5, or 200/10mg/kg) for 2 weeks. At 30 minutes after the final treatment, they were challenged with 3mL/kg ethanol (15mL of 20% in water/kg). The blood concentrations of alcohol and acetaldehyde were analyzed up to 7 hours postchallenge. Hepatic mRNA expression levels of alcohol-metabolizing enzymes, alcohol dehydrogenase (ADH), cytochrome P450 type 2E1 (CYP2E1), and aldehyde dehydrogenase (ALDH), were determined by real-time polymerase chain reaction. Additional rats were challenged with ethanol and, 60 minutes later, administered GEY/RES to evaluate alcohol clearance. Pretreatment with GEY/RES for 2 weeks reduced the blood concentrations of alcohol and acetaldehyde in a dose-dependent manner, lowering by 29.5% and 54.6% at the highest dose (200/10mg/kg), respectively. The expressions of mRNAs for ADH and ALDH, the major alcohol-metabolizing enzymes, were markedly increased in the livers of rats administered GEY/RES for 2 weeks, whereas CYP2E1 mRNA was suppressed. Postchallenge treatment with GEY/RES enhanced the alcohol clearance rate by lowering blood concentrations of alcohol and acetaldehyde by 24% and 26.6%, respectively, for the highest dose group. GEY/RES remarkably eliminated 2,2-diphenyl-1-picrylhydrazyl hydrate radical and FeCl3-mediated lipid peroxidation in vitro and attenuated hepatic lipid accumulation following ethanol administration in vivo. Therefore, it is suggested that GEY/RES reduces the blood concentrations of alcohol and acetaldehyde not only by modulating alcohol-metabolizing enzymes, but also by exerting its antioxidant activity, and that GEY/RES could be a promising candidate for improvements of alcoholic hangover.

Protective Effect of Anthocyanins against the Hepatotoxicity of Carbon Tetrachloride in Rats

Sunhee Shin,Dongsun Park,Min-Jung Jang,Jeong Hee Jeon,Namgil O,Jugyeong Song,Jinsoo Lee,Byung-Yul Kim,Seong Soo Joo,Seock-Yeon Hwang,Yun-Bae Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.3

Reddish purple-colored fruits and vegetables contain high level of anthocyanins, which are well known to exert antioxidant, cardioprotective and hepatoprotective actions. In the present study, the effect of purified anthocyanin on the liver injury and dysfunction induced by carbon tetrachloride (CCl₄) was investigated. Male Sprague-Dawley rats were injected intraperitoneally with CCl₄ at a dose of 1.1 mL/㎏ to induce hepatic damage. The rats were orally administered with anthocyanin (50, 160 or 500 ㎎/㎏) 4 hr and 30 min prior to as well as 4 hr and 20 hr after CCl₄ challenge. Four hr following final anthocyanin treatment (24 hr after CCl₄ injection), time-course of blood concentration of intravenously-injected bromosulphalein were examined. In addition, blood parameters of hepatic injury in accordance with histopathological findings were analyzed. The biliary excretion time and clearance of bromosulphalein were greatly delayed by CCl₄ injection, which were recovered by anthocyanin (160-500 ㎎/㎏). CCl₄ drastically increased the blood markers of hepatotoxicity, and caused centrilobular congestion, hepatocytic degeneration, lipid droplets and inflammatory cell infiltration, leading to disintegration of hepatic cords The increased biochemical parameters and hepatic lesions induced by CCl₄ were significantly attenuated by anthocyanin treatment. The results suggest that anthocyanin might prevent liver dysfunction induced by CCl₄, and that anthocyanin could be a potential candidate for the prevention of hepatic disorders.

Hepatoprotective Effect of a Preparation of Combined Glutathione- Enriched Yeast and Rice Embryo/Soybean Extracts

Dongsun Park,Heon-Sik Lee,Tae Myoung Kim,Jugyeong Song,Jeong Hee Jeon,Sunhee Shin,Seong Soo Joo,Hyoung Kook Park,Won Kyung Lee,Jin Tae Hong,Yun-Bae Kim 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.1

The effects of GEY+RES, a preparation of combined glutathione-enriched yeast (GEY) and rice embryo/soybean (RES) extracts (20:1), on carbon tetrachloride (CCl₄)-induced hepatic injury were investigated in male Sprague-Dawley rats. The rats were orally administered with GEY+RES at low (GEY+RES=50+2.5 ㎎/㎏), medium (100+5 ㎎/㎏) or high (200+10 mg/kg) doses for 2 weeks, followed by intraperitoneal challenge with CCl₄ (1 mL/㎏) 30 min after the final treatment. Twenty four hours later, initial biliary excretion and time-course of blood concentration of intravenously-injected bromosulphalein were examined. In addition, blood parameters of hepatic injury in accordance with histopathological findings were analyzed. The biliary excretion time and clearance of bromosulphalein were greatly delayed by CCl₄ injection, which were recovered by GEY+RES in a dose-dependent manner, resulting in decreases to a half level by medium and high doses. CCl₄ drastically increased the blood markers of hepatotoxicity, and caused centrilobular congestion, hepatocytic degeneration, lipid droplets and inflammatory cell infiltration, leading to disintegration of hepatic cords. The increased biochemical parameters and hepatic lesions induced by CCl₄ were significantly attenuated by GEY+RES treatment. The results suggest that GEY+RES might prevent liver dysfunction induced by CCl₄, and that GEY+RES could be a potential candidate for the prevention of hepatic disorders.

Effect of Repeated Administration of Silk Peptide on the Immune System of Rats

Jae Myun Ryu,Tae Myoung Kim,Im Kwon Seo,Seongho Yeon,Woo-Taek Lim,Jeong-Yong Lee,Seock-Yeon Hwang,Namgil O,Jugyeong Song,Jinsoo Lee,Yun-Bae Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.3

In order to elucidate the possibility of influence on the immune system, silk peptide was administered to rats for 4 weeks. Male and female Sprague-Dawley rats were orally treated with low (500 ㎎/㎏) or high (2,000 ㎎/㎏) doses of silk peptide, and complete blood counts, organ weights, histopathology and cellularity of immune systems were examined. Silk peptide did not cause clinical signs, although the body weight gain at the high dose was slightly reduced. In hematological analysis, the number of white blood cells (WBC) and differential counts, the number of red blood cells (RBC) and RBC indices, and the number of platelets and mean platelet volume (MPV) were not affected by the administration of silk peptide. Especially, there were no differences in the number and rate (%) of immune cells such as lymphocytes as well as monocytes and granulocytes between vehicle and silk peptide-treated groups. Silk peptide neither affected the thymus and spleen weights, in addition to the cellularity in thymus, nor induced gross and microscopic lesions of thymus, spleen and lymph node. Taken together, 4-week administration of silk peptide up to 2,000 ㎎/㎏, the upper-limit dose in repeated-dose toxicity study, did not exhibit adverse effects on the immune systems, leading to no observed adverse effect level (NOAEL) higher than 2,000 ㎎/㎏. Without influence on the production, maturation and death process of immune cells at 20-fold dose of clinical dose (100 ㎎/㎏), silk peptide was evaluated to be a practically non-toxic material.

Effects of Sweet Potato Fractions on Alcoholic Hangover and Gastric Ulcer

Jwa Jin Kim,Chang Whan Kim,Dongsun Park,Sunhee Shin,Jeong Hee Jeon,Min-Jung Jang,Hyeong-Jin Ji,Namgil O,Jugyeong Song,Jinsoo Lee,Byung-Yul Kim,Ehn-Kyoung Choi,Seong Soo Joo,Seock-Yeon Hwang,Yun-Bae Ki 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.2

Reddish purple-colored fruits and vegetables contain high level of anthocyanins, which are well known to exert antioxidant, cardioprotective and hepatoprotective actions. In the present study, the effects of sweet potato fermentation filtrate (SPF) and its fractions on alcoholic hangover and gastric ulcer were investigated. In order to evaluate antihangover effect, male Sprague-Dawley rats were orally administered with SPF (240 or 750 ㎎/㎏) 30 min prior to or 60 min after oral challenge with 3 mL/㎏ of ethyl alcohol (15 mL/㎏ of 20% in water), and the blood concentration of alcohol were analyzed up to 5 hours. Pretreatment with 240 and 750 mg/kg of SPF significantly reduced the blood ethanol concentration by 20-25% and 50-60%, respectively, which was superior to the effect of the Hovenia dulis Thunb extract. In addition, posttreatment of SPF enhanced the alcohol-clearance rate to 2.8 and 3.1 folds at doses of 240 and 750 ㎎/㎏, respectively. In comparison, liquid portion of SPF exerted similar antihanover effect to SPS, whereas the effect of purified anthocyanins was negligible, indicating that active ingredients are included in the liquid portion. For the assessment of antiulcer activity, fractions of SPF were orally treated 30 min before oral administration of absolute ethanol (3 mL/㎏) and intravenous injection of 1% Evan's blue (0.5 mL/㎏). One hour later, the ulcer index (㎜ of ulcer lesions) was measured, and dye leakage on the gastric wall was quantified by extracting the dye with formamide. SPF and its liquid portion (300 ㎎/㎏) near-fully prevented the ethanol-induced gastric ulcer and significantly reduced the Evan's blue leakage, while the purified anthocyanins were ineffective. Among subfractions, buthanol fraction of SPFliquid portion exerted a substantial antiulcer activity, which was superior to water and methanol fractions. Taken together, it was demonstrated that SPF possesses antihangover and antiulcer activities, and that buthanol fraction of SPF-liquid portion contains active ingredients. Therefore, it is suggested that SPF or its buthanol fraction could be a potential candidate for the attenuation of hangover and gastric ulcers induced by excessive drinking of alcohol, although the action mechanisms remain to be clarified.