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      • A Study on the Effect of Physical Education in Chinese University on Student Satisfaction

        Junjun Yang(Junjun Yang),Li Wang(Li Wang) 동아시아무역학회 2022 Journal of East Asian Trade(JEAT) Vol.4 No.2

        Purpose - This study aims to understand the level and location of physical education at Chinese universities. Through the analysis of the Chinese sports industry and the government’s sports education policy, we will analyze the development process of Chinese sports education and the implementation process of the Chinese government’s sports policy to provide basic data on the future direction of Chinese sports education. Design/Methodology/Approach - The research method of this study analyzed the effect of physical education in China on student satisfaction through theoretical analysis and policy analysis on the Chinese sports industry and government and analyzed the development process of physical education and the implementation process of the Chinese government. Findings - The results of this study show the positive effect of physical education at Chinese universities on students’ satisfaction and academic achievement. Therefore, Chinese universities must devote more time and resources to physical education to improve students’ health and academic achievement. Research Implications - The limitation of this study is that when measuring the level of participation in sports activities, it may be difficult to grasp the overall level of participation in sports activities because it uses a qualitative measurement method or only measures some sports participation items. In addition, it can be difficult to generalize because the sample size is small to generalize the study results or because the study subject is limited to a specific university or region. Therefore, considering these limitations, finding ways to supplement the research results while actively utilizing them is necessary.

      • KCI등재

        MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase

        Yuting Wang,Lixin Lin,Yong Huang,Junjun Sun,Xueming Wang,Peng Wang 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.12

        Purpose: Adipogenic differentiation of adipose tissue-derived mesenchymal stem cells (AMSCs) is critical to many disease-relateddisorders, such as obesity and diabetes. Studies have demonstrated that miRNA-138 (miR-138) is closely involved in adipogenesis. However, the mechanisms affected by miR-138 remain unclear. This work aimed to investigate interactions between miR-138and lipoprotein lipase (LPL), a key lipogenic enzyme, in AMSCs. Materials and Methods: Human AMSCs (hAMSCs) isolated from human abdomen tissue were subjected to adipogenic differentiationmedium. Quantitative real-time polymerase chain reaction and Western blot assay were applied to measure the expressionsof miR-138, LPL, and the two adipogenic transcription factors cytidine-cytidine-adenosine-adenosine-thymidine enhancerbinding protein alpha (C/EBPα) and peroxisome proliferator-activated receptor gamma (PPARγ). The relationship between miR-138 and LPL was predicted utilizing the miRTarBase database and validated by dual luciferase reporter assay. Results: Showing increases in C/EBPα and PPARγ expression levels, hAMSCs were induced into adipogenic differentiation. Duringadipogenesis of hAMSCs, miR-138 expression was significantly downregulated. Overexpression of miR-138 by transfection inhibitedhAMSCs adipogenic differentiation in vitro. Mechanically, LPL was a target of miR-138. LPL expression was upregulated duringadipogenesis of hAMSCs, and this upregulation was reversed by miR-138 overexpression. Functionally, silencing of LPL by transfectionexerted similar inhibition of the expressions of C/EBPα and PPARγ. Meanwhile, LPL ectopic expression was able to partlyabolish the suppressive effect of miR-138 overexpression on adipogenic differentiation of hAMSCs. Conclusion: Upregulation of miR-138 inhibits adipogenic differentiation of hAMSCs by directly downregulating LPL.

      • SCIESCOPUSKCI등재

        Dietary L-arginine Supplementation Improves Intestinal Function in Weaned Pigs after an Escherichia coli Lipopolysaccharide Challenge

        Liu, Yulan,Han, Jie,Huang, Jingjing,Wang, Xiaoqiu,Wang, Fenglai,Wang, Junjun Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.12

        This study was conducted to determine whether L-arginine (Arg) supplementation could improve intestinal function in weaned pigs after an Escherichia coli lipopolysaccharide (LPS) challenge. Treatments included: i) non-challenged control (CONTR, pigs fed a control diet and injected with sterile saline); ii) LPS-challenged control (LPS, pigs fed the same control diet and challenged by injection with Escherichia coli LPS); iii) LPS+0.5% Arg (pigs fed a 0.5% Arg diet and challenged with LPS); and iv) LPS+1.0% Arg (pigs fed a 1.0% Arg diet and challenged with LPS). On d 16, pigs were administrated with LPS or sterile saline. D-xylose was orally administrated at 2 h following LPS challenge, and blood samples were collected at 3 h following LPS challenge. At 6 h post-challenge, pigs were sacrificed and intestinal mucosa samples were collected. Supplementation of Arg attenuated LPS-induced damage in gut digestive and barrier functions, as indicated by an increase in ileal lactase activity, and duodenal and ileal diamine oxidase activities (p<0.05). Arg administration also prevented the increase of jejunal malondialdehyde content and the decrease of ileal superoxide dismutase activity by LPS challenge (p<0.05). Furthermore, the jejunal nitric oxide level and inducible nitric oxide synthase activity were also improved after Arg supplementation (p<0.05). These results indicate that Arg supplementation has beneficial effects in alleviating the impairment of gut function induced by LPS challenge.

      • KCI등재

        Development of Grain Boundary Character Distribution in Medium‑Strained 316L Stainless Steel During Annealing

        Weijiu Huang,Shanshan Yuan,Linjiang Chai,Luyao Jiang,Haiding Liu,Fangjun Wang,Dongzhe Wang,Junjun Wang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.2

        A medium-strained (13% cold-drawing) 316L austenitic stainless steel rod was subjected to annealing between 750 and1100 °C. Electron backscatter diffraction and electron channeling contrast imaging techniques were jointly employed toinvestigate the development of microstructures and grain boundary character distribution during the annealing treatments. Results show that annealing at temperatures below 900 °C does not evidently change microstructures of the as-drawn specimen. Full recrystallization is observed after the temperature reaches 1000 °C and the deformation microstructures are replacedby fine and uniform recrystallized grains. A large number of annealing twins (with Σ3 misorientation) are produced by therecrystallization, leading to markedly increased fractions of special boundaries (fSB). The maximum fSB is found to be 67.3%in the present study. In addition, for the recrystallized grains, rapid growth is noticed at relatively high temperatures due toeasy migration of grain boundaries.

      • KCI등재

        Long Noncoding RNA Expression Profiling During the Neuronal Differentiation of Glial Precursor Cells from Rat Dorsal Root Ganglia

        Yunfei Dai,Wei Ma,Tong Zhang,Jinwei Yang,Chenghao Zang,Kuangpin Liu,Xianbin Wang,Jiawei Wang,Zhen Wu,Xingkui Zhang,Chunyan Li,Junjun Li,Xiangpeng Wang,Jianhui Guo,Liyan Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

        Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC_000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG, and also provides a resource for lncRNA studies in the field of cell differentiation.

      • KCI등재

        High-Conductivity SiO2-Matrix B-Doped Si-NC Thin Films by Following Ion-Beam Treatment

        Junjun Huang,Weiyan Wang,Jie Yang,Yongzhen Tan,Wei Chen,Tianyu Ge,Yajun Zhang,Min Gao,Zhenming Chen 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.6

        In this work, further ion-beam was performed on SiO2-matrix B-doped Si-NC (SBC) thin films in order to enhance conductivity. The effect of ionbeamtype on the electrical properties of SBC thin films was investigatedsystematically. The results indicated that the conductivities of SBC thinfilms were significantly improved by both argon and hydrogen ion-beamtreatments, and the higher the hydrogen ion ratio, the higher theconductivity of SBC thin films. The conductivity of SBC thin films wasincreased from 1.82 × 10−6 S/cm to 3.2 × 10−3 S/cm with followinghydrogen-ion-beam treatment. The change in conductivity of SBC thinfilms was most possibly resultant from the ion-beam treatment facilitatingthe formation of higher superficial order and lower defects. An alternativemethod was proposed to prepare high-conductivity SBC thin films, whichmay be applied to other heterogeneous thin films.

      • KCI등재

        Nicotine Induces the Expression of C-Reactive Protein via MAPK-Dependent Signal Pathway in U937 Macrophages

        Junjun Mao,Juntian Liu,Xiaoming Pang,Ming Li,Jinyan Song,Chunjie Han,Di Wu,Shuyue Wang 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.5

        Atherosclerosis is an inflammatory disease in the vessel wall. Nicotine, a major component of cigarette smoke, is an independent risk factor for cardiovascular diseases including atherosclerosis. As an inflammatory molecule, C- reactive protein (CRP) participates in atherogenesis. Although it has been confirmed that CRP level in smoking patient is significantly higher than non-smokers and cigarette withdrawal,it is unknown whether nicotine induces CRP expression in macrophages. The present study was to observe effect of nicotine on CRP production and the related signal pathway in U937 macrophages. The results showed that nicotine significantly increased mRNA and protein expression of CRP in U937 macrophages in time- and concentration-dependent ways. Nicotinic acetylcholine receptor (nAChR) blocker hexamethonium, MEK1/2 inhibitor PD98059, p38 MAPK inhibitor SB203580 and NF-B inhibitor PDTC almost completely abolished nicotine-induced CRP expression in mRNA and protein levels in U937 macrophages. The further study indicated that hexa-methonium, PD98059, and SB203580 significantly inhibited ERK1/2 and p38 MAPK phosphorylation. These demonstrate that nicotine has ability to induce CRP ex-pression in macrophages through nAChR-ERK1/2/p38 MAPK-NF-B signal pathway, which contributes to better understanding of the pro-inflammatory and pro-atherosclerotic effects of nicotine in cigarette smokers.

      • SCIESCOPUS

        Investigation on energy dissipation and its mechanism of coal under dynamic loads

        Feng, Junjun,Wang, Enyuan,Shen, Rongxi,Chen, Liang,Li, Xuelong,Xu, Zhaoyong Techno-Press 2016 Geomechanics & engineering Vol.11 No.5

        The energy dissipation of coal under dynamic loads is a major issue in geomechanics and arising extensive concerns recently. In this study, dynamic loading tests of coal were conducted using a split Hopkinson pressure bar (SHPB) system, the characteristics of dynamic behavior and energy dissipation of coal were analyzed, and the mechanism of energy dissipation was discussed based on the fracture processes of coal under dynamic loads. Experimental results indicate that the energy dissipation of coal under dynamic loads has a positive linear correlation with both incident energy and dynamic compressive strength, and the correlation coefficients between incident energy, dynamic compressive strength and the energy dissipation rate are 0.74 and 0.98, respectively. Theoretical analysis demonstrates that higher level of stress leads to greater energy released during unstable crack propagation, thus resulting in larger energy dissipation rate of coal under dynamic loads. At last, a semi-empirical energy dissipation model is proposed for describing the positive relationship between dissipated energy and stress.

      • KCI등재

        Greeting, Function, and Music: How Users Chat with Voice Assistants

        Ji Wang,Han Zhang,Cen Zhang,Junjun Xiao,Seung Hee Lee 한국감성과학회 2020 감성과학 Vol.23 No.2

        Voice user interface has become a commercially viable and extensive interaction mechanism with the development of voice assistants. Despite the popularity of voice assistants, the academic community does not utterly understand about what, when, and how users chat with them. Chatting with a voice assistant is crucial as it defines how a user will seek the help of the assistant in the future. This study aims to cover the essence and construct of conversational AI, to develop a classification method to deal with user utterances, and, most importantly, to understand about what, when, and how Chinese users chat with voice assistants. We collected user utterances from the real conventional database of a commercial voice assistant, NetEase Sing in China. We also identified different utterance categories on the basis of previous studies and real usage conditions and annotated the utterances with 17 labels. Furthermore, we found that the three top reasons for the usage of voice assistants in China are the following: (1) greeting, (2) function, and (3) music. Chinese users like to interact with voice assistants at night from 7 PM to 10 PM, and they are polite toward the assistants. The whole percentage of negative feedback utterances is less than 6%, which is considerably low. These findings appear to be useful in voice interaction designs for intelligent hardware.

      • KCI등재

        Switching Motion Control of Aircraft Skin Inspection Robot Using Backstepping Scheme and Nussbaum Disturbance Observer

        Congqing Wang,Junjun Jiang,Xuewei Wu,Linfeng Wu 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.6

        In this paper, a backstepping control using switching strategy is proposed for the motion control of the aircraft skin inspection robot with double frame in the presence of external disturbances. The inspection robot equipped with a CCD camera and an ultrasonic sensor can alternately adsorb and move on the aircraft surface. The influence of the external disturbances is obvious in the switching motion. The disturbances are efficiently estimated using a Nussbaum disturbance observer (NDO), and the disturbance observer errors are uniformly ultimately bounded. Then, the backstepping control method is used to design the motion controller. The stability of the closed-loop robot system is proved by Lyapunov analysis through the average dwell time method .The tracking errors and the disturbance observer errors are semi-globally uniformly bounded using the proposed control schemewith NDO,and NDO is compared with Super-twisting disturbance observer. Finally, simulation results illustrate that the proposed control scheme with NDO can achieve satisfactory tracking performance under the external disturbance.

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