Cytological, genetic, and proteomic analysis of a sesame (Sesamum indicum L.) mutant Siyl‑1 with yellow–green leaf color

Tong‑Mei Gao,Shuang‑Ling Wei,Jing Chen,Yin Wu,Feng Li,Li‑Bin Wei,Chun Li,Yan‑Juan Zeng,Yuan Tian,Dong‑Yong Wang,Hai‑Yang Zhang 한국유전학회 2020 Genes & Genomics Vol.42 No.1

Background Both photosynthetic pigments and chloroplasts in plant leaf cells play an important role in deciding on the photosynthetic capacity and efficiency in plants. Systematical investigating the regulatory mechanism of chloroplast development and chlorophyll (Chl) content variation is necessary for clarifying the photosynthesis mechanism for crops. Objective This study aims to explore the critical regulatory mechanism of leaf color mutation in a yellow–green leaf sesame mutant Siyl-1. Methods We performed the genetic analysis of the yellow-green leaf color mutation using the F2 population of the mutant Siyl-1. We compared the morphological structure of the chloroplasts, chlorophyll content of the three genotypes of the mutant F2 progeny. We performed the two-dimensional gel electrophoresis (2-DE) and compared the protein expression variation between the mutant progeny and the wild type. Results Genetic analysis indicated that there were 3 phenotypes of the F2 population of the mutant Siyl-1, i.e., YY type with light-yellow leaf color (lethal); Yy type with yellow-green leaf color, and yy type with normal green leaf color. The yellowgreen mutation was controlled by an incompletely dominant nuclear gene, Siyl-1. Compared with the wild genotype, the chloroplast number and the morphological structure in YY and Yy mutant lines varied evidently. The chlorophyll content also significantly decreased (P < 0.05). The 2-DE comparison showed that there were 98 differentially expressed proteins (DEPs) among YY, Yy, and yy lines. All the 98 DEPs were classified into 5 functional groups. Of which 82.7% DEPs proteins belonged to the photosynthesis and energy metabolism group. Conclusion The results revealed the genetic character of yellow-green leaf color mutant Siyl-1. 98 DEPs were found in YY and Yy mutant compared with the wild genotype. The regulation pathway related with the yellow leaf trait mutation in sesame was analyzed for the first time. The findings supplied the basic theoretical and gene basis for leaf color and chloroplast development mechanism in sesame.

Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita

Wenjie Tong(Wenjie Tong),Junying Li(Junying Li),Wenfeng Cong(Wenfeng Cong),Cuiping Zhang(Cuiping Zhang),Zhaoli Xu(Zhaoli Xu),Xiaolong Chen(Xiaolong Chen),Min Yang(Min Yang),Jiani Liu(Jiani Liu),Lei Yu 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations.

Study on the performance and mechanism of Cu(II) adsorption by porous ceramics prepared from tailings

Tong Zhang,Shaofeng Zhu,Guangsheng Fei,Yuanhang Ma 한양대학교 청정에너지연구소 2022 Journal of Ceramic Processing Research Vol.23 No.6

In this study, industrial waste tailings were used as the main raw material to prepare porous ceramic by injection molding. The porous ceramic samples were surface treated by γ-Aminopropyl triethoxysilane solution to be used as adsorbent to removeCu(II) from solution. The effects of sintering technology on properties of porous ceramic samples were studied. Meanwhile,the effects of test conditions on the adsorption characteristics of samples were also studied. The samples were analyzed by Xray diffraction, FTIR, and SEM. Atomic absorption spectrophotometer was used to determine the changes of Cu(II)concentration in the aqueous solution before and after adsorption. The results show that the compressive strength of thesamples is increased with the increase of sintered temperature and extension of holding time. The average pore diameters ofthe samples are decreased and concentrated between 0.1 and 0.2mm with the increase of sintered temperature. The removalrates and the adsorption capacities of the samples to Cu(Ⅱ) are increased with the increase of solution temperature, increaseof pH value of the solution and the extension of the test time.

On the photoluminescence of multi-sites Ce³⁺ in T-phase orthosilicate and energy transfer from Ce³⁺ to Tb³⁺

Tong, Xubo,Zhang, Xinmin,Wu, Luyi,Zhang, Hongzhi,Seo, Hyo Jin Elsevier 2018 JOURNAL OF ALLOYS AND COMPOUNDS Vol.748 No.-

<P><B>Abstract</B></P> <P>Ce<SUP>3+</SUP>, Tb<SUP>3+</SUP> co-doped T-phase orthosilicate Ba<SUB>1.2</SUB>Ca<SUB>0.8</SUB>SiO<SUB>4</SUB> phosphors were prepared by means of solid state reactions. The synthesized samples were investigated using XRD and PL emission and excitation spectra. The emission spectra for Ce<SUP>3+</SUP> doped Ba<SUB>1.2</SUB>Ca<SUB>0.8</SUB>SiO<SUB>4</SUB> phosphors show broad bands in the 320–550 nm spectral region. The excitation spectra exhibit several absorption peaks in the range of 250–400 nm. Both the emission and excitation spectra are dependent on the excitation or monitoring wavelengths. We attribute these results to Ce<SUP>3+</SUP> ions occupying two different crystallographic sites (i.e., Ce I and Ce II). The Ce<SUP>3+</SUP>, Tb<SUP>3+</SUP> co-doped Ba<SUB>1.2</SUB>Ca<SUB>0.8</SUB>SiO<SUB>4</SUB> phosphors show Tb<SUP>3+</SUP>-related line emissions in the 475–600 nm spectral region and Ce<SUP>3+</SUP>-related band emissions in the 350–525 nm spectral region when the 4<I>f</I> → 5d transition of Ce<SUP>3+</SUP> is excited (λ<SUB>ex</SUB> = 345 nm), indicating that energy transfer from Ce<SUP>3+</SUP> to Tb<SUP>3+</SUP> takes place. Moreover, the emission intensity of <SUP>5</SUP>D<SUB>4</SUB> → <SUP>7</SUP>F<SUB>5</SUB> transition for Ba<SUB>1.00</SUB>Ce<SUB>0.02</SUB>Tb<SUB>0.08</SUB>Li<SUB>0.10</SUB>Ca<SUB>0.8</SUB>SiO<SUB>4</SUB> is 30 times stronger than that of Ba<SUB>1.04</SUB>Tb<SUB>0.08</SUB>Li<SUB>0.08</SUB>Ca<SUB>0.8</SUB>SiO<SUB>4</SUB> sample under 345-nm UV excitation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PL spectra are dependent on excitation or monitoring wavelengths. </LI> <LI> Ce<SUP>3+</SUP> ions occupy two different crystallographic sites (i.e., Ce I and Ce II). </LI> <LI> Efficient energy transfer from Ce<SUP>3+</SUP> to Tb<SUP>3+</SUP> takes place. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

A STABILIZED CHARACTERISTIC FINITE VOLUME METHOD FOR TRANSIENT NAVIER-STOKES EQUATIONS

TONG ZHANG 한국전산응용수학회 2011 Journal of applied mathematics & informatics Vol.29 No.5

In this work, a stabilized characteristic finite volume method for the time-dependent Navier-Stokes equations is investigated based on the lowest equal-order finite element pair. The temporal differentiation and advection term are dealt with by characteristic scheme. Stability of the numerical solution is derived under some regularity assumptions. Optimal error estimates of the velocity and pressure are obtained by using the relationship between the finite volume and finite element methods.

Metabolomics reveals potential biomarkers in the rumen fluid of dairy cows with different levels of milk production

Zhang, Hua,Tong, Jinjin,Zhang, Yonghong,Xiong, Benhai,Jiang, Linshu Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1

Objective: In the present study, an liquid chromatography/mass spectrometry (LC/MS) metabolomics approach was performed to investigate potential biomarkers of milk production in high- and low-milk-yield dairy cows and to establish correlations among rumen fluid metabolites. Methods: Sixteen lactating dairy cows with similar parity and days in milk were divided into high-yield (HY) and low-yield (LY) groups based on milk yield. On day 21, rumen fluid metabolites were quantified applying LC/MS. Results: The principal component analysis and orthogonal correction partial least squares discriminant analysis showed significantly separated clusters of the ruminal metabolite profiles of HY and LY groups. Compared with HY group, a total of 24 ruminal metabolites were significantly greater in LY group, such as 3-hydroxyanthranilic acid, carboxylic acids, carboxylic acid derivatives (L-isoleucine, L-valine, L-tyrosine, etc.), diazines (uracil, thymine, cytosine), and palmitic acid, while the concentrations of 30 metabolites were dramatically decreased in LY group compared to HY group, included gentisic acid, caprylic acid, and myristic acid. The metabolite enrichment analysis indicated that protein digestion and absorption, ABC transporters and unsaturated fatty acid biosynthesis were significantly different between the two groups. Correlation analysis between the ruminal microbiome and metabolites revealed that certain typical metabolites were exceedingly associated with definite ruminal bacteria; Firmicutes, Actinobacteria, and Synergistetes phyla were highly correlated with most metabolites. Conclusion: These findings revealed that the ruminal metabolite profiles were significantly different between HY and LY groups, and these results may provide novel insights to evaluate biomarkers for a better feed digestion and may reveal the potential mechanism underlying the difference in milk yield in dairy cows.

Diversity and geographic variation of endosymbiotic bacteria in natural populations of the pea aphid (Acyrthosiphon pisum) in China

Zhang Li,Cao Ya,Zhang Li-Jun,Wang Meng-Yao,Wang Xiao-Tong,Yang Xing-Zhuo,Duan Ting-Yu,Yuan Ming-Long 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Bacterial symbionts in aphids are known to benefit the insect host and associated with aphid’s ecological adaptation. The pea aphid (Acyrthosiphon pisum), an important legume pest worldwide, carries at least eight endosymbionts, providing a model system to study insect–bacteria interactions. However, species diversity and geographic variations of endosymbionts are unknown in Chinese populations; therefore, we characterized symbiont communities and diversity of 27 pea aphid samples from 13 geographic populations of China. Via amplicon high-throughput sequencing and diagnostic PCR, we found that bacterial communities of Chinese populations were dominated by Proteobacteria and Firmicutes. Among eight known endosymbionts, five (Buchnera, Serratia, Hamiltonella, Regiella, and Rickettsia) were detected by both methods, with a specific geographical distribution. The obligate symbiont, Buchnera, was present in all aphid samples, while the four facultative symbionts showed a significant geographic variation. Each population was randomly infected with distinct endosymbionts, ranging from three to five species. Serratia and Rickettsia showed relatively higher abundance in central regions of China, Regiella was predominant in eastern and western China, whereas Ham iltonella showed an extremely low abundance and was absent in four populations. Samples grouped by altitudes showed a significant diversity difference, whereas there was no significant difference between red and green body colors. Bacterial community structures of the Chinese pea aphid populations were mainly influenced by environmental factors, other than body colors. These data can guide the development of potential biocontrol techniques against this aphid.

Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study

Zhang Pei-Pei,Liang Su-Xia,Wang Hua-Lun,Yang Kun,Nie Shao-Chen,Zhang Tong-Mei,Tian Yuan-Yuan,Xu Zhao-Yuan,Chen Wei,Yan Ying-Bin 한국통합생물학회 2021 Animal cells and systems Vol.25 No.5

The aim of this study was to compare the functional characteristics of mesenchymal stromal cells (MSCs) from a sheep model of traumatic temporomandibular joint (TMJ) fibrous and bony ankylosis. A sheep model of bilateral TMJ trauma-induced fibrous ankylosis on one side and bony ankylosis on the contralateral side was used. MSCs from fibrous ankylosed callus (FAMSCs) or bony ankylosed callus (BA-MSCs) at weeks 1, 2, 4, and 8 after surgery were isolated and cultured. MSCs derived from the bone marrow of the mandibular condyle (BM-MSCs) were used as controls. The MSCs from the different sources were characterized morphologically, phenotypically, and functionally. Adherence and trilineage differentiation potential were presented in the ovine MSCs. These cell populations highly positively expressed MSC-associated specific markers, namely CD29, CD44, and CD166, but lacked CD31 and CD45 expressions. The BA-MSCs had higher clonogenic and proliferative potentials than the FA-MSCs. The BA-MSCs also showed higher osteogenic and chondrogenic potentials, but lower adipogenic capacity than the FA-MSCs. In addition, the BA-MSCs demonstrated higher chondrogenic, but lower osteogenic capacity than the BM-MSCs. Our study suggests that inhibition of the osteogenic and chondrogenic differentiations of MSCs might be a promising strategy for preventing bony ankylosis in the future.

Benevolence (上医云集)

Tong Zhang 한국디자인학회 2021 한국디자인학회 국제초대전도록 Vol.2021 No.11

Study on the measurement accuracy of circular transmission line model for low-resistance Ohmic contacts on III-V wide band-gap semiconductors

Tong Liu,Rong Huang,Fangsen Li,Zengli Huang,Jian Zhang,Jianping Liu,Liqun Zhang,Shuming Zhang,An Dingsun,Hui Yang 한국물리학회 2018 Current Applied Physics Vol.18 No.7

The accuracy and error propagation for determining the low specific contact resistance of Ohmic contacts on IIIV wide band-gap semiconductors based on the circular transmission line model have been analyzed and the validity of this method is discussed in detail. The accuracy is more susceptible to the factors including data fitting method, electrical measurement technique and contact area correction. By using the equations of the original circular transmission line model to extract the fitting parameters, the calculation accuracy is much improved and the inapplicability of the linear least-square fitting is prevented. To further improve the accuracy, a four-probe current-voltage measurement technique was adopted to reduce the parasitic series resistances and the uncertainty bound, especially for the Ohmic contact with low sheet resistance of the semiconductor. Moreover, we have studied the size effect of contact pads of patterns and demonstrated that contact area correction is necessary for the semiconductor with high sheet resistance. A comprehensive error analysis is also performed to fully understand all the impact factors on this advanced method of specific contact resistance measurement, which is benefit for device performance evaluation and failure analysis.