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Jumi Park,Kyonghwa Song,Sohyeon Oh,Taewon Son,Jun Lee,Ayoung Park,Hyun Ji Kim,Youngsoo Jun,Changwook Lee 한국구조생물학회 2017 Biodesign Vol.5 No.3
Vac8p is a vacuolar protein that plays pivotal roles in both vacuole inheritance and the formation of nucleus vacuole junction (NVJ) in yeast. The Vac8p directly interacts with Atg13p, a component of the autophagy machinery, and mediates cytoplasm-to-vacuole targeting (Cvt) pathway, resulting in the maturation of aminopeptidase I (Ape1p). Here, we coexpressed and purified Saccharomyces cerevisiae Vac8p complexed with Atg13p in Escherichia coli bacteria cells, and crystallized the complex proteins under the condition of 25% (v/v) PEG 400, 100 mM Tris pH 8.5, 2% (v/v) Ethylene glycol, 2% (w/v) PEG 3350, 1.5% (w/v) PEG 20000, 5 mM DTT at 293K. X-ray diffraction data of the crystals were collected to 2.9 Å resolution at the synchrotron radiation. The crystals belong to the orthorhombic space group P2 1 2 1 2 1 with unit cell parameters a = 62.7 Å, b = 92.4 Å, and c = 139.9 Å. The asymmetric unit contains one Vac8p-Atg13p heterodimer with a corresponding V M of 2.92 Å 3 Da -1 and solvent content of 57.8%.
신경성 폭식증 여성의 내적 성찰 과정 연구 : 거부당한 몸과 마음에 대한 자기위로, 그리고 자기수용
주현정,고주미,황순찬,이명수 이화여자대학교 사회복지연구소 2022 사회복지 실천과 연구 Vol.19 No.2
본 연구는 두 명의 여성이 경험한 신경성 폭식증의 의미와 신경성 폭식증에 대한 이들의 내적 성찰 과정을 탐구하여, 섭식장애를 겪는 이들에 대한 이해의 폭을 확장하는 데 목적이 있다. 이를 위해 기존의 이론이나 가설을 전제하지 않고 오직 연구자와 연구참여자 사이의 변증법적 대화를 통해 인터뷰를 진행하는 Kleining의 함부르크 접근법(Hamburg Approach)을 활용하였다. 연구 과정에서 나타난 연구참여자의 내적 성찰 내용을 분석한 결과, 총 4개의 헤드라인이 도출되었다. 4개의 헤드라인은 ‘수용되지 못한 자신 : 몸에 대한 거부와 정서적 거부’, ‘수용되기 위한 노력 : 섭식절제와 관계 갈망’, ‘노력의 실패: 폭식과 구토의 사이클’, ‘새로운 통찰’이다. 각 헤드라인은 ‘거부당한 자신에 대한 자기위로, 그리고 자기수용’ 이라는 최종 헤드라인으로 수렴되었다. 이러한 연구 결과를 바탕으로 신경성 폭식증의 위험에 대비할 수 있는 다각적인 사회복지 서비스의 필요를 제언하였다. The purpose of this study was to expand the understanding of bulimia nervosa, by exploring the experiences of two women with eating disorders and their introspection process. To this end, by using the Hamburg Approach of Qualitative Heuristic Research, the interview was conducted without presupposing existing theories or hypotheses, but only through a dialectical conversation between the researcher and the participants, and the participants’ introspection were analyzed. The contents were organized into a total of four headlines as follow: 'The Unaccepted Self: Rejection of the Body and Emotions', 'The Effort to Be Accepted: Temperance of Eating and Relationship Cravings', 'Failed Efforts: Cycle of Binge-eating and Vomiting', and 'New Insights'. Following the heuristic research method of identifying one coherent structure from the entire data, the participants responses were condensed into the final headline, 'Self-consolation and Self-acceptance for the Rejected Body and Mind'. This study highlights the need for a multifaceted social welfare service that can better cope with the danger of eating disorders, which are underestimated compared to its lethality, as well as the need for innovative intervention methods for counseling treatment.
Gil, Chang-Hyun,Lee, Ji-Heon,Seo, Joseph,Park, Soon-Jung,Park, Zewon,Kim, Jumi,Jung, A-Ra,Lee, Won-Young,Kim, Jong-Soo,Moon, Sung-Hwan,Lee, Hoon-Taek,Chung, Hyung-Min Kluwer Academic Publishers 2015 Biotechnology letters Vol.37 No.6
<P>Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study. To overcome this, hemangioblast studies have focused on utilizing human embryonic stem cell (hESC) derivatives but current methods are cell-line dependent. Single cell dissociation of a hESC colony quickly led to cell death in most hESC lines due to enzyme treatment which, in turn, reduced induction potential and hemangioblast differentiation efficiency. Therefore, we sought to effectively improve the process of cell dissociation that is adaptable to various hESC lines and increase the initial induction potential of embryoid body (hEB). As a result, we determined an effective cell dissociation method through a comparison study involving various reagents which demonstrated successful dissociation regardless of cell line and enhanced hemangioblast differentiation efficiency.</P>
( Zahra Zahid Piracha ),( Hyun Woong Lee ),( Umar Saeed ),( Jumi Kim ),( Hyeonjoong Kwon ),( Yong-joon Chwae ),( Jin Hong Lim ),( Sun Park ),( Ho-joon Shin ),( Kyongmin Kim ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Sirtuin 2 (Sirt2), an NAD+-dependent protein deacetylase, deacetylates tubulin, AKT, and other proteins. Previously, we showed that Sirt2 isoform 1 (Sirt2.1) increased replication of hepatitis B virus (HBV). Methods: Here, we show that HBV replication upregulates expression of Sirt2 primary and alternatively spliced transcripts, and their respective isoforms 1, 2, and 5. Since Sirt2 isoform 5 (Sirt2.5) is a catalytically inactive nuclear protein with a splicedout nuclear export signal (NES), we speculated that its different localization may affect its activity. Results: Overexpression of Sirt2.5 reduced expression of HBV mRNAs, replicative intermediate DNAs, and covalently closed circular DNA (cccDNA), an activity opposite to that of Sirt2.1 and Sirt2.2. Unlike the Sirt2.1-AKT interaction, the Sirt2.5-AKT interaction was weakened by HBV replication. Unlike Sirt2.1, Sirt2.5 activated the AKT/GSK-3ß/ß-catenin signaling pathway very weakly and independently of HBV replication. When the NES and an N-terminal truncated catalytic domain were added to the Sirt2.5 construct, it localized in the cytoplasm and increased HBV replication (like Sirt2.1 and Sirt2.2). Chromatin immunoprecipitation assays revealed that more Sirt2.5 was recruited to cccDNA than Sirt2.1. Also, recruitment of histone lysine methyltransferases (HKMTs) such as SETDB1 and SUV39H1, EZH2, and PR-Set7, and their respective transcriptional repressive markers H3K9me3, H3K27me3, and H4K20me1, to cccDNA increased in Sirt2.5-overexpressing cells. Among these, the Sirt2.5-PR-Set7 and -SETDB1 interactions increased upon HBV replication. Conclusions: These results demonstrate that Sirt2.5 reduces cccDNA levels and viral transcription through epigenetic modification of cccDNA via direct and/or indirect association with HKMTs, thereby exhibiting anti-HBV activity.
국소 최적성과 순차 기준을 바탕으로 한 검파 기법: 2. 성능 분석
최상원,강현구,이주미,박소령,김선용,송익호,Choi Sang Won,Kang Hyun Gu,Lee Jumi,Park So Ryoung,Kim Sun Yong,Song Iickho 한국통신학회 2005 韓國通信學會論文誌 Vol.30 No.10C
이 논문에서는, 약한 신호를 검파하는 데에 알맞도록 1부에서 얻은 순차 검파 방식의 성능을 고정 표본 검파방식, 순차 확률비 검파 방식, 끝을 자른 순차 확률비 검파 방식의 성능과 견주어 본다. 제안한 순차 검파 방식은 순차 확률비 검파 방식과 견주어 볼 때, 얼개가 같거나 덜 복잡하고 신호를 더 빠르게 검파할 때가 많다. 아울러, 제안한 순차 검파 방식은 고정 표본 검파 방식과 끝을 자른 순차 확률비 검파 방식과 견주어 얼개가 덜 복잡하거나 같고 필요한 관측수가 늘 적다. In this paper, the performance of the sequential detection scheme proposed in Part 1 is compared with that of the fixed sample size (FSS) test, sequential probability ratio test (SPRT), and truncated sequential probability ratio test (TSPRT). The proposed sequential detection scheme requires less complexity and, in most cases, smaller sample size than the SPRT. It is also observed that the proposed sequential detection scheme has always lower complexity and smaller sample size than the FSS test and TSPRT.
( Kyung-ho Park ),( Sang Hoon Joo ),( Ji-hye Seo ),( Jumi Kim ),( Goo Yoon ),( Young-joo Jeon ),( Mee-hyun Lee ),( Jung-il Chae ),( Woo-keun Kim ),( Jung-hyun Shim ) 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.1
Licochalcone H (LCH) is a phenolic compound synthetically derived from licochalcone C (LCC) that exerts anticancer activity. In this study, we investigated the anticancer activity of LCH in human skin cancer A375 and A431 cells. The 3-(4,5-dimethylthiazol- 2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay was used to evaluate the antiproliferative activity of LCH. Cell cycle distribution and the induction of apoptosis were analyzed by flow cytometry. Western blotting assays were performed to detect the levels of proteins involved in cell cycle progression, apoptosis, and the JAK2/STAT3 signaling pathway. LCH inhibited the growth of cells in dose- and time-dependent manners. The annexin V/propidium iodide double staining assay revealed that LCH induced apoptosis, and the LCH-induced apoptosis was accompanied by cell cycle arrest in the G1 phase. Western blot analysis showed that the phosphorylation of JAK2 and STAT3 was decreased by treatment with LCH. The inhibition of the JAK2/STAT3 signaling pathway by pharmacological inhibitors against JAK2/STAT3 (cryptotanshinone (CTS) and S3I-201) simulated the antiproliferative effect of LCH suggesting that LCH induced apoptosis by modulating JAK2/STAT3 signaling.