The Safety and Efficacy of Daclatasvir and Asunaprevir with Chronic HCV Genotype 1 Infection and Hemodialysis

( Myeong Jun Song ),( Jung Hyun Kwon ),( Soon Woo Nam ),( Tae Hee Lee ),( Young Woo Kang ),( Ji Woong Jang ),( Seok Hyun Kim ),( Byung Seok Lee5 ),( Sea Hwan Lee ),( Hong Soo Kim ),( Ji Hoon Kim ),( S 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: We aimed to evaluate the efficacy and safety of Daclatasvir (DAV) and Asunaprevir (ASV) in patients with chronic hepatitis C virus (HCV) and chronic renal failure (CRF). Methods: 22 chronic hemodialysis patients with HCV infection at 12 medical centers have prospectively enrolled from Feb, 2016 to Feb, 2017 (NCT02580474). Of those, 9 patients who were followed 12 weeks after treatment ended were included. We evaluated the virological responses at each week 4, 12, 24 and the 12-week sustained virologic response (SVR12). The tolerability and safety of patients were also evaluated. Results: Of these 20 patients, there had no resistance-associated variant of NS5A (NS5A RAVs) and 2 patients showed indeterminate NS5A RAVs. 12 patients (54.5%) completed the 24 weeks treatment of DAV and ASV. 6 patients (27.2%) have continued, but 4 patients discontinued study prior to 12 weeks. Overall, all patients (n=9) achieved SVR12. Virologic response at week 4, 12 and 24 showed 94.4% (17/18), 93.3% (14/15), and 100% (12/12) respectively. DCV and ASV were well tolerated among the majority of patients and discontinuation of the treatment due to adverse events (hypertension, bradycardia, leukemia) was occurred in 3 patients. In two patients with indeterminate NS5A RAVs, one achieved SVR12 but the other showed viral breakthrough and discontinued treatment. Conclusions: In this study, DAV and ASV combination therapy for chronic hemodialysis patients with HCV infection achieved high sustained virological response with few adverse events. Close monitoring of safety and tolerability may be necessary when treating chronic hepatitis C patients with CRF receiving DCV and ASV.

서초동(서울시 서초구)의 거미상

김주필(Joo Pil Kim),정혜리(Hye ri Jeong),김대희(Dae Hee Kim),박동우(Dong Woo Park),서재웅(Jae Woong Seo),이현우(Hyun woo Lee) 한국거미연구소 2017 한국거미 Vol.33 No.1

2016년 3월 31일부터 7월 31일까지 서울특별시 서초구 서초동에 위치한 서울고등학교 교정 일대를 서울고등학교 환경생물부 부원들이 채집 조사한 결과 20과 43속 53종의 거미가 조사 연구되어 이에 보고하는 바이다. From March 31st to July 31st in 2016, members of an environmental biology club at Seoul High School located in Seocho-gu, Seoul, Dae-Hee Kim, Dong-Woo Park, Jae-Woong Seo and Hyun-woo Lee, collected 20 families 43 genera 53 species of spiders and report the result of the study here.

Streptokinase와 Plasminogen의 활성 복합체에 대한 생화학적 특성

이현재,전형균,이해진,김태성,김연웅 慶尙大學校 1988 論文集 Vol.27 No.2

Fig 1과 Fig 2의 실험 결과에 의하면 streptokinase에 의한 plasminogen의 활성화 기작은 먼저 streptokinase와 plasminogen 결합하여 복합체를 만들고 그 다음 다른 분자의 streptokinase에 의한 plasminogen을 streptokinase에 의한 plasmin으로 활성화 한다고 생각되었다.그리고 원래 효소활성이 없는 streptokinase량이 plasminogen보다 과량으로 존재하게 되면 전체 esterolytic 활성도는 plasminogen 의 량에 의존하게 될 것이다.Fig 3의 실험에서 streptokinase plasminogen 복합체의 정량적인 구성 비율을 조사하였더니 plasminogen 1㎍에 0.5㎍의 streptokinase가 결합하였다. 이는 보고된 plasminogen의 분자량을 87.000(10) 그리고 streptokinase분자량이 47.000(6)으로 계산하면 각각 한 분자씩 결합하고 있음을 알 수 있었다. 이 결과는 Fig. 1의 결과를 보충할 수 있는 data이다. streptokinase plasminogen 복합체의 생화학적 특성을 조사하기 위하여 복합체만의 활성을 assay 할 수 있는 방법을 고안하였다. 실험결과 soybean trysin inhibitor를 사용하면 plasminogen의 활성화에 의하여 생성된 plasmin의 esterolytic 활성은 완벽하게 저해할 수 있었으나 streptokinase plasminogen 복합체의 활성은 저해하지 못하였으므로 soybean trypsin inhibitor를 첨가하여plasmin활성을 제거하여 순수한 복합체만의 활성을 측정할 수 있었다.(Fig.5).그러나 pancreatic tyrpsin inhibitor는 Plasmin 뿐만 아니라 streptokinase plasminogen복합체의 활성을 모두 저해할 수 있었으므로 이 inhibitor 는 복합체의 활성을 측정하는데 사용하지 않았다. (Fig.4). 두가지 inhibitor의 복합체에 대한 상이한 저해 효과는 복합체의 활성화 자리 (active site)에 두 inhibitor 가 결합하고난 후 기질이 출입할 수 있는 공간의 넓이에 의한 효과인 것으로 생각되었다 (Table 1).따라서 soybean trypsin inhibitor을 이용한 assay는 원래의 생화확적 특성에 영향이 없는 것으로 생각되었다. 복합체의 esterolytic 반응은 빠른 첫 번째 반응 그리고 그 이후 늦은 두 번째 반응으로 진행되는 biphasic한 형태를 보여 주었다(Fig.7).이 반응 기작은 알려진 serine protease들이 acyl중간체을 이용한 biphasic 반응 기작과 유사한 형태이었다. 활성화 자리에 위치하고 있는 여러 작용기들을 확인하기 위한 기초 실험으로써 pH에 따른 esterolytic활성을 측정한 결과 최대 활성을 보이는 pH는 7.5이었다.(Fig.8). 이 근처의 pKa 값을 가지고 아미노산은 histidine 종류을 생각할 수있을 것이다. The kinetic data suggest that streptokinase interacts with plasminogen to become an active complex which in turn. activates another molecule of plasminogen to plasmin. This complex was fromed stoichiometrically at 1:1 molar ratio. A series of experiment were carried out to find the condition that the streptokinase plasminogen complex activities free from plasmin could be monitered. The soybean trypsin inhibitor wsas used to assay the activity of streptokinase-plasminogen complex. The lack of inhibiton of esterase activity by soybean trypsin inhibitor was thought to be the size of the protein in active site. The esterolytic reaction by streptokinase-plasminogen complex showed biphasic pattern. The pH profile showed typical bell-shape and optimal pH for streptokinas-plasminogen complex showed biphasic pattern. The pH profile showed a typical bell-shape and optimal pH for streptokinas-plasminogen complex was 7.5.

人蔘根 腐病發生에 미치는 病原과 線蟲의 關聯性

李敏雄,申鉉成,沈在郁 동국대학교 1985 論文集 Vol.24 No.-

4 roots of 2 year old ginseng were planted in a small pot trateing with fungal pathogens, Fusarium solani, nematodes isolated from the ginseng (Panax ginseng C.A. Meyer) cultured soil and the two mixed inoculum respectively. Rate of infestation and nematodes distributed around the harvested host plant after 40 days was investigated. To obtained results were as follows: Each group was treated with F. solani, nematodes and mixed inoculum showed higher disease index by infestation from 3.3 to 3.8 while control group was healthy showing lower disease index of 1.2. The nematodes identified were 4 genera in control group and F. Solani inoculated group. 11 genera of nematodes including Xiphinema spp were distributed in the nematodes inoculated pot, and their numbers were higher in the population. Especially Radophalus spp, Tylenchulus spp, Meloidogyne spp, Xiphinema spp and Ditylenchus spp were numerous in their population density, but Belonolimus spp and Hemicyciophora spp were lower. Fourteen genera of nematodes were distributed in the mixed inoculation pot than only the nematodes treated group. and also the population was higher in the mixed inoculation group. The more populated genera is number were Ditylenchus spp, Paratylenchus spp, Paratylenchus spp. Hemicycliophora spp, Meloidogyne spp, Helicotylenchus spp and Tylenchorhynchus spp, but Hirschmanniella spp was less in the population density.

Phage 항원성에 미치는 trypsin의 영향

이현국,양남웅,서재홍 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.1

In order to investigate the effect of trypsin(TP) on the phage antigenicity, the Klebsiella phage-host cell system and sheep red blood cells were used, and the results were as follows. The neutralization velocity of Tp-treated phage(Tp phage) by anti-normal phage serum was faster than that of normal phage. And, the neutralization of normal phage by anti-Tp phage serum was remarkably worse than that of Tp phage. The normal phage - neutralizing activity of the anti-normal phage serum absorbed with Tp phage was very weak. However, the anti-Tp phage serum absorbed with normal phage still considerably neutralized the Tp phage. The cold phage hemagglutination(CPH) by normal and Tp phage was most favourable when the reactions are carried out with each phage-correspondig antiserum. The anti-normal phage serum absorbed with Tp phage and the anti-Tp phage serum absorbed with normal phage still formed, with each corresponding phage, the CPH. The neutralization of Tp phage, by anti-normal phage serum, Passed two times through host bacteria and that of Tp phage, by anti-Tp phage serum, Passed three times through host bacteria were similar to the neutralization of normal phage by each antiserum. If the Tp phage is passed two times or three times through host bacteria, the forming capability of CPH by each phage bacame similar, under the anti - normal and anti - Tp phage sera, to that of normal phage. The absorption of Tp phage to host bacteria was a little faster than that of normal phage. If the Tp tretment of phage is done for 48hours at 37℃, the surviving titer was reduced to around 40%.

진단 초음파를 이용한 정상 성인의 정중신경 측정

이정아,임길병,이홍재,정웅태,황현오 최신의학사 2003 最新醫學 Vol.46 No.11-12

체육수업 몰입의 촉진 및 방해 요인 탐색

이현주,김기웅 이화여자대학교 동작과학연구소 2007 이화체육논집 Vol.10 No.-

The purpose of this study is to find out the factors affecting students' flow in the physical class based on the flow experience theory. To do this, this research is to search the factors that facilitate or interfere flow and if such factors show at different degrees by sex. If you can make the conditions for the students to experience flow in their physical classes, physical education at school can be not only the activities of students' active participation but also self-directed ones. For the research, the researcher handed out questionnaires to 480 male and female students in middle schools and high schools in Seoul and analysed 417 of them. The collected data have been analyzed through frequency analysis and two-way ANOVA. Also, of the questions in the questionnaire, open questions have been analyzed through inductive content analysis. The result of this research shows that the factors that facilitate flow in physical classes are preference of specific activities, enjoyment, freedom, peer, the feeling of capability and confidence, physical, emotional and health, the feeling of achievement and competence, instructor, environment, evaluation factor, etc. And the factors that interfere flow in physical classes are dissatisfaction of the content of the class, environment, physical and conditional limit, evaluation, the lack of the feeling of capability and confidence, peer, instructor, management of the class, punishment factor, etc. 15 factors influence flow have been comprised through quantitative analysis and the two-way analysis of variance. They show that there are differences by sex and class in the factors of preference of specific activities, the feeling of achievement and competence, physical and condition, the feeling of capability and confidence, freedom, the lack of the feeling of capability and confidence, environment, friend, evaluation, instructor, enjoyment, and others. There was difference by sex in the dissatisfaction of the content of the class factor and there was not difference by sex and class in management of the class and punishment factor(P<.05). Such results tells that most of the factors influence How in physical classes have difference by sex or class and that these various factors should be considered according to the sex and the class.

위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동

이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.

사이버 여행기업 이용자의 만족도 분석

이응규,김용완,권현재 한국관광정보학회 2003 觀光情報硏究 Vol.- No.13

This is an empirical study on the user's satisfaction to cyber travel agencies. The digital trend has pur our travel market into a transition period. The number of internet sites related with domestic travel have been growing day by day. But they don't provide the consumers with enough information of the products they offer. They are nothing better than a home page introducing themselves. This study is designed to establish the theoretical backgrounds of the cyber travel agencies and their present situations inn Korea through satisfaction analysis in order to find out solutions to the problems they face, eventually developing the models for the internet business. This study analyses recent cyber travel market all of the world and suggests strategies for cyber travel agencies. Main suggestions to be successful cyber travel agencies are as follows; Cyber travel agencies have to (1) respond to changing environment on internet industry and user's needs; (2) develop new travel products and websites to appeal special interset segments; (3) cooperate with business partner to elevate competitiveness in cyber travel market.

공동탐사 시스템의 비교에 관한 연구

이호재,조병서,허웅,김현중 明知大學校 産業技術硏究所 1995 産業技術硏究所論文集 Vol.14 No.-

In this study, we report the characteristics of three Kinds of Radar Exploration Systems, which are used in geological cavity detection, for effective use. The systems are RAMAC, PEMSS and KAIST CW. We repeated measurements each system for same geological model. The AMAC System has a unique capability in finding fracture zones from the reflected waves, and represents the cavity as a hyperbola because the cavity responds as a point source. In the PEMSS systems the relative velocity and attenuation of the electromagnetic wave which varies by geological medium represents geological structures. The main merit of the PEMSS system is cavity localization. The location of the cavity is determined the depths of cavity signals in each offset data. The KAIST CW system also detects a cavity from the attenuation pattern of continuous waves. This uses the phenomenon of 180'phase change of the wave when it propagates the cavity, producing the high attenuation at that point. Our study shows that the RAMAC is suitable in cavity scanning, the PEMSS is adaptive in precise geology exploration, and KAIST CW is best in cavity detection.