Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122의 생산과 그 특성

최용석,옥승호,유주현,배동현 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.1

토양으로부터 분리한 Metarrhizium anisopliae (Metschn.) Sorok이 생산하는 biopolymer를 정제하여 분석하고, 그의 생산조건을 검토하였다. 탄소원으로 mannitol, 질소원으로는 polypepton을 사용했을 때 가장 많은 biopolymer를 생산하였고 K_2HPO_4 및 CaCl_2를 첨가함으로서 생산량이 더욱 증가하였다. 또한 이 biopolymer를 정제하여 그 분자량과 구성성분을 검토한 결과 Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122는 분자량이 1.7×10 exp (6)이고, C, H의 구성비가 1:2이며 미량의 N이 검출되었으나, 주 구성성분은 glucose와 galactose의 환원당으로 이루어져있는 전혀 새로운 biopolymer인 것으로 나타났다. To produce biopolymer, Metarrhizium anisopliae (Metschn.) Sorok was cultured in a medium containing glucose 1.0%, sucrose 2.0%, soluble starch 1.0%, yeast extract 0.5%, polypeptone 0.5%, K_2HPO_4 0.1%, MgSO_4·7H_2O 0.02%. The culture broth was centrifuged and the polymer was harvested by adding methanol to the culture supernatant. When three times of methanol was added, the polymer was coagulated and precipitated. Then it was further purifued through successive SK-1B, SA-20P, HP-20 column chromatographies. This polymer was designated as Biopolymer YU-122. C:H ratio of this Biopolymer YU-122 was 1:2 and small amount of N is detected by CHN analyzer. Glucose and galactose are main components of this polymer. Average molecular weight of this biopolymer was 1.7×10 exp (6) by Sepharose 4B gel permeation chromatography. Optimal condition for biopolymer production was investigated. When 5% of mannitol was used as a carbon source, and polypepton as a N source, highest productivity of biopolymer was achieved. C/N ratio as nutrient was also a major factor in polymer production and its optimal ratio was 3.

한국인 제2형 당뇨병 환자에 있어 지속적 피하 인슐린 주입법 치료시 Lispro insulin와 Regular insulin의 효과 비교

유재등,박봉안,류하근,류주성,김정수,김치훈,박선민,최수봉 건국대학교 의과학연구소 2001 건국의과학학술지 Vol.11 No.-

Objective: The Lispro insulin has faster onset of effects and shorter duration than the human regular insulin, an analog of human insulin. The differences in the pharmacodynamics between the two types of insulin in the continuous subcutaneous insulin infusion(CSII) with insulin pump for treating the Korean Type 2 DM patients were analyzed. The effects on the regulation of blood sugar, the amount of insulin injected, and the frequency of hypoglycemia were compared between the lispro insulin and the regular human insulin. Method and Material: One hundred and three Korean Type 2 DM patients were randomly divided into two groups, 49 Lispro insulin and 54 Regular insulin.(Table 1) Both groups were treated for 14 days. Also at a set time before meal, insulin was injected as bolus, using a portable insulin pump, in both groups, 15 minutes before for lispro insulin and 30 minutes before for human insulin The HbA1C, C-peptide, AC/PC, height and weight were measured for each patients at the time of admission. The peripheral blood sugar level and insulin injection profile were recorded 7 times per day for each patients. Results: After treating for 7 days and 14 days, the blood sugar level normalized in both groups. There weren't any significant differences in blood sugar levels in both groups at point of the study, and also there weren't any differences in the frequency of hypoglycemia. In order to maintain the normalized blood sugar level, the lispro insulin group had higher total daily insulin requirement than the regular insulin group after 14 days of treatment (0.90±0.35 vs 0.73±0.39u/kg/day, p<0.05) This difference is probably due to the difference in basement insulin requirements rather than prandial insulin requirements (0.32±0.1 vs 0.43± 0.09u/kg/day, p<0.001). Discussion: In the hospitalized Korean Type 2 DM patients with CSII treatment, continuous subcutaneous insulin infusion therapy using lispro insulin has convenience in usage compared to the regular insulin. However, there were not any differences in the blood sugar levels. Also lispro insulin requires more insulin in order to maintain normal blood sugar level, which is probably due to the increase in the basal requirement. Also there were not any difference in the frequency of hypoglycemia.

심한 저칼륨혈증을 동반한 갑상선 중독성 주기성 마비 2례

유승진,최승필,최경호,오동렬,황주일,나병호,김세경 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Thyrotoxic periodic paralysis occurs primarily in Orientals with an overwhelming male preponderance and a higher association of specific HLA antigens. Clinical manifestations include onset after high carbohydrate ingestion or heavy exertion with progressive symmetric weakness leading to flaccid paralysis of the extremities and other muscle groups, lasting several hours. Other precipitating facters include trauma, infection, administration of epinephrine, thyroid hormone, or corticosteroid. If hypokalemia is present, potassium administration may help abort the attack. Although propranolol can be efficacious in preventing further episodes, the only definitive treatment is euthyroid state. The pathophysiology is still controversal, but reflects altered potassium and calcium dynamics as well as certain morphologic characteristics within the muscle unit itself. We discribed two patients with thyrotoxic periodic paralysis who had a no familial history of paralysis and had a decreased level of serum potassium during attack.

暗渠排水에서 Grove의 公式에 關한 考察

周載洪,李裕根 진주산업대학교 1979 論文集 Vol.17 No.-

Grover's formula had played an important role to develop the theory for descent of the undergroundwater level in the underdrainage. Fall velocity of the underground water level gives a big effect to the cultivation of crops in the wet land. As a result of Grover's theory into pratice, Dr Yamazaki Hujio had found that was a big error. As applicating in experiment plots to Grover's fomula, obtained results are as follows. 1. As you see in the table(1), the result of experiment of Dr Yamazaki Hujio was existent higher value of v than the result of Grover's fomula. The result of fomula(Yc=127e-0.0496t) which resolved to apply a big value of v is existent a calculation mistake. it has been found a phenomenon that the water go not down into the underground, conversely going up. 2. As you see in the table(1),(2),(3) compared with Grover's fomula(1),(2) the value of y_c is a big error in regard to the value of t. 3. As you see in the table(3), (4) the result of applicating in my experiment plots, Grover's fomula is approximate with regard to the actual value.

신뢰성있는 멀티캐스트 통신에서의 흐름 제어

유소영,서주하 강원대학교 정보통신연구소 1999 정보통신논문지 Vol.3 No.-

In this paper, we propose flow control scheme for reliable multicast communication. When packet loss is occurred by buffer overflow on the transmission path or receivers, the receivers detect the loss and send the NAK. In this case NAK-based retransmission is said to be effective because it can avoid implosion of NAK packets. Buffer overflow in receivers may occurred constantly, if the transmission rate is not decreased. So the flow control should be imposed to prevent further overflow. In this paper, we proposed a flow control scheme for NAK-based flow control scheme in reliable multicast communications. When the NAK are generated by receivers, sender decreases the transmission rate preventing the fall of throughput by excessive decrease. We show that the proposed scheme improves throughput compared with the case of no flow control.

人參成分 抽出에 관한 硏究

柳洲鉉,卞裕亮,劉承坤 연세대학교 산업기술연구소 1978 논문집 Vol.8 No.1

The amount of ethanol extract from ginseng was about 20.3% (w/w)and the yield of saponins from the extract was 5.5% (w/w). The optimum concentration of solvent was 60% ethanol. The optimum extraction time and temperature were respectively 2 hr and 80℃ when 0.5g of sample (Φ:0.25∼0.42mm) was added to 300ml of solvent. The empirical equation for the extraction of components was as follow: Y(θ,T,φ)=8log(Θ??100)-45log(T??×100)-60log(Φ??×100)+38 The partical size of sample had largely influence on the extraction yield, and the extraction mechanism was considered as diffusion.

Verticillium sp.가 생산하는 cellulase의 정제 및 그 성질

유주현,홍대희,양융,진효상 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1

The cellulase of verticillium sp. was purified to investigate its some properties. The enzyme purification was carried out by ammonium sulfate fractionation, and column chromatography of Sephadex G-50, G-100 and DEAE Sephadex A-25, with its specific activity increased to 17 fold. The molecular weight was estimated to be 46,000 by gel filtration. The optimum pH and temperature were pH 7 and 50℃, respectively. The enzyme was stable over the range of pH5-7 at 50℃ for 1 hour. But half of its activity was lost after 2 hour. The km value was calculated to be 1.2mg of CMC/㎖.

견고 구외 골신장술을 이용한 구순구개열 환아의 치험례 : CASE REPORT

유난영,박호원,,김성민,이주현,서현우 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.4

구순구개열 환자에서는 이른 시기에 시행된 수술로 인한 구순이나 구개부의 반흔 형성으로 섭식장애나 발음장애를 동반한 상악의 열성장이 나타나게 된다. 때로는 집중적인 교정치료 후에도 심한 상악골 저형성증을 보이며, 이러한 경우 성장이 완료된 후에 골이식을 동반한 악교정 수술로 상악골을 전방이동시켜 안모의 개선을 도모하기도 한다. 그러나 이러한 상악골의 전방이동은 연조직의 과도한 신장으로 인한 술후회귀현상, 추가적인 골이식이 필요하다는 한계를 가지고 있다. 골신장술은 이러한 한계점을 극복하는 최신 치료방법으로 구순구개열 환자, 두개골 융합증을 나타내는 환자 등에서 상악골을 포함한 두개 안면골의 개선에 많이 이용되고 있다. 특히 구외장치를 이용하는 골신장술은 골신장기간 중에 견인 방향의 조절이 보다 쉬우며, 충분한 양의 골신장이 가능하여 보다 좋은 결과를 얻을 수 있다. 본 증례는 상악 열성장을 보이는 구순구개열 6세 7개월의 여자 환아로, 변형된 구내 프로씨 1형 골전단술(modified Le Fort I osteotomy) 후 두개골을 고정원으로 이용하는 견고 구외 골신장술을 통해 상악골을 전진시켜 양호한 결과를 얻었기에 이를 보고하는 바이다. Patients with cleft lip and palate present severe maxillary hypoplasia due to scar of lip and palate, often accompanied by compromised mastication, speech abnormalities. Sometimes maxillary hypoplasia persist even though active orthodontic treatment was done. In theses cases, patients born with cleft lip and palate will be potential candidates for maxillary advancement with bone grafting after growth to correct the functional deformities and improve aesthetic facial proportions. But, maxillary advancement using standarf surgical approaches has several limitations: increased relapse tendency after maxillary advancement, necessity of additional bone graft and mandibular setback surgery. Distraction osteogenesis is current treatment modality to overcome these limitations, thus has become popular for treatment of maxillary hypoplasia associated cleft lip and palate, craniosyntosis. Especoally, rigid external distraction, contrary to internal device, has adbantages; better vector control of osteotomized segment, effective traction of the bony segments, the ease of the application and removal the distraction device. This study showed that relatively successful result could be generated by using rigid external distraction osteogenesis(RED) in the case of cleft lip and palate with severe maxillary hypoplasia, 6 years 7 months old.

소의 시상하부내에서 Vasopressin과 Oxytion분비세포의 분포에 관한 면역조직화학적 연구

유명철,김종중,장인엽,문정석,정주현,김흥중,조사선 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

This study was done to observe the distributions of Oxytocinergic and Vasopressinergic neurons in the hypothalamus of the cow. For the immunohistochemical staining, sections were reacted with the following sequence of solutions: monoclonal anti-vasopressin and anti-oxytocin, biotinylated anti-mouse IgG, avidin-biotin peroxidase complex, and Ni-DAB. The results observed under light microscope were summarized as follows. Our immunohistochemical investigations reveal both vasopressinergic and oxytocinergic neurons in the supraoptic nucleus(SON), paraventricular nucleus(PVN), accessory supraoptic nucleus (ASN), periventricular nucleus(PN), suprachiasmatic nucleus(SCN), and arcuate nucleus(AN), but these two types of neurons were mainly distributed in the PVN and SON and the ratio of the number of vasopressinergic neurons to that of oxytocinergic neurons was 1:1.30 in the PVN, and 1:1.00 in the SON. These two types of neurons were round, oval and spindle-shaped and cell size was 30-55um.

구강내 미생물에 대한 서양산 고추냉이(Armoracia rusticana) 뿌리 추출물의 항균효과

유난영,박호원,이주현,서현우 대한소아치과학회 2006 大韓小兒齒科學會誌 Vol.33 No.3