한국인 갑상선 수질암 환자에서 RET 원종양유전자 점돌연변이 양상

김형훈,김현진,정윤재,민용기,이명식,이문규,김광원,기창석,김종원,정재훈 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.4

연구배경: 갑상선 수질암의 25∼30%는 유전성으로 발현되는데, MEN 2A, MEN 2B 또는 가족성 수질암의 형태로 나타난다. RET 원종양유전자의 점돌연변이가 유전성 갑상선 수질암의 발생에 중요한 역할을 하므로, 진단 당시의 연령이나 가족력 유무에 관계없이 모든 갑상선 수질암 환자나 또는 RET 변이가 발견된 수질암 환자의 가족 구성원들에서 RET 변이 검색을 하여야 한다. 또한 일부 문헌에서 RET 변이의 양상에따라 임상상이 다르게 표현됨이 보고된 바 있다. 이에 저자들은 한국인 갑상선 수질암 환자에서 RET 원종양유전자의 점돌연변이의 양성률을 알아보고, 변이 양상에 따른 임상상의 차이가 있는지를 알아보고자 본 연구를 시행하였다. 방법: 치근 7년간 본원에서 갑상선절제술을 통해 갑상선 수질암으로 진단받은 29예에서 RET 원종양유전자의 점돌연변이 검사를 시행하였다. 29예의 평균연령은 39세(20∼60세)이었고, 남자 7예, 여자 22예 이었다. 이들의 말초혈액에서 genomic DNA를 분리하고, 특이 시발차를 이용하여 RET 원종양유전자의 exon10, 11, 13, 14, 16부위를 증폭하였다. 증폭된 부위를 자동염기서열분석기를 이용하여 직접 분석하였다. 양성으로 나온 경우는 모든 가족 구성원을 대상으로 RET 변이 유무를 검색하였다. 결과: 대상 환자 29예 중 9예 (31%)에서 RET 원종양유전자 점돌연변이가 발견되었다 RET 변이가 발견된 9예 (남자 3예, 여자 6예)의 평균 연령은 33세 (20∼51세)로 RET 변이가 발견되지 않은 20예의 평균연령 42세(24∼60세)보다 의미 있게 적었다. RET 변이가 발견된 9예 중 MEN 2A가 5예, 가족성 수질암이 1예, 그리고 산발성 수질암이 3예이었고, MEN 2B는단 1예도 진단되지 않았다. MEN 2A 5예 중 4예는 exon 11의 codon 634번(C634R 2예, C634Y 2예)에서, 그리고 나머지 1예는exon 10의 codon 618번 (C618R)에서 변이가 각각 발견되었다. 가족성 수질암 1아는 codon 634번(C634W)에서, 산발성 수질암 3예도 모두 codon 634번 (C634y 2예, C634s 1예)에서 각각 변이가 발견되었다. RET변이 양상 또는 위치에 따른 임상상의 차이는 발견할 수 없었다. 결론: 갑상선 수질암 환자 31%에서 RET 원종양유전자의 점돌연변이를 발견할 수 있었다. 유전성 수질암 중 가족성 수질암 1예를 제외하고 나머지 5예는MEN 2A이었다. 저자들의 5예와 지금까지 국내에서 보고 된 7예를 합친 국내 MEN 2A 12예 중 75% (9/12)는 exon 11의codon 634번(C634R 4예, C634y 4예, C634w 1예)에서, 그리고 나머지 25% (3/12)는 exon 10의 codon618번(C618R 2예, C618s 1예)에서 변이가 발견되었다. 국내에서는 codon 634과 codon 618 두 곳에만 국한된 양상이었고, codon 634에서의 C634R 변이는 1/3에서만 나타났다. 비록 본 연구에서는 제한된 환자 수 때문에 변이 양상과 임상상의 관계를 규명할 수 없었지만, 향후 많은 수의 환자를 대상으로 전향적인 연구를 시행하여 genotype-phenotype 관계 규명을 하는 것이 필요하다. Background: Medullary thyroid carcinomas (MTC) have been reported as hereditary in about 25 -30% of cases. The identification of germline mutation in RET proto-oncogene is important in the diagnosis of hereditary MTC, and occurs in three forms: MEN 2A, MEN 2B and familial MTC (FMTC). To evaluate the prevalence of the relationship of RET proto-oncogene mutation and genotype-phenotype was studied in Korean patients with MTC. Methods: Genomic DNA was obtained from 29 patients, with MTC, who underwent a total thyroidectomy, between 1997 and 2003, at the Samsung Medical Center. There were 7 male and 22 female patients, with an average age of 39, ranging from 20 to 60 years. Exon 10, 11, 13, 14 and 16 of the RET proto-oncogene were amplified, with specific primers, using PCR. A sequencing analysis was performed on the PCR product using an automatic sequencing analyzer. Results: Nine of the 29 patients (31%) were identified as having RET mutations. The average age of these 9 patients was 33 years, ranging from 20 to 51, with a female to male ratio of 2. Five patients had MEN 2A and one had FMTC, with the other 3 thought to have non-hereditary (sporadic) MTC. The 4 patients with MEN 2A had RET mutations on codon 634 of exon 11 (2 patients, C634R; 2 patients, C634Y) and the other patient on codon 618 of exon 10 (C618R). One patient with FMTC had a mutation on codon 634 (C634W). Three patients with sporadic MTC had RET mutations on codon 634 (2 patients, C634Y; 1 patient, C634S). However, no genotype- phenotype relationship could be found, due to the limited number of patients. Conclusion: Thirty-one percent (9/29) of the patients with MTC had RET proto-oncogene mutations. Three-quarters (9/12) of the Korean patients with MEN 2A, including another 7 patients reported in 3 papers in Korea, had RET mutations on codon 634 of exon 11 (4 patients, C634R; 4 patients, C634Y; 1 patient, C634W), but a quarter (3/12) had mutations on codon 618 of exon 10 (2 patients, C618R; 1 patient, C618S). Although no relations could be found between the genotypes and phenotypes, extensive prospective studies will be required to verify this (J Kor SOC Endocrinol 18:360-370, 2003).

로드셀을 이용한 고속 감자 중량계량법

김상철,임종환,김귀식,김성근,양종훈 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

Potatos, together with tangerines, are one of the major agricultural products in Jeju, and the production account for more than 30% of the domestic production. Recently some kinds of sorting machine for potatos are available, but they are not extensively used because their performance is not satisfactory and/or they are very expensive. This paper presents a load based high speed weighting method for sorting the potatos. This method is based on the fact that the linear momentum of a potato is proportional to the mass of it. The usefulness of the method is demonstrated with sets of experiments.

동종 골수이식 후 만성 이식편대숙주반응 환자에서 발생한 폐렴구균에 의한 수막뇌염 1예

신완식,김병욱,유진홍,김동집,김춘추,박종원,이종욱,김동욱,강문원,김양리 대한감염학회 1993 감염 Vol.25 No.3

Graft-versus-host disease (GVHD) is a frequent complication after bone marrow transplantation. Infectious complications are common in GVHD patients due to defect in cell-mediated immunity. A rare case of S. pneumoniae meningoencephalitis occured in a patient with extensive form of chronic GVHD after allogeneic bone marrow transplantation. He was immediately treated with full dosage of ceftriaxone and ampicillin. He suffered from various complications such as sepsis, acute renal failure, atelectasis, and seizure. Despite of aggressive treatment, he died probably due to renal shutdown and massive subacute cerebral infarction of left cerebral hemisphere. This report showed two unusual and rare features. First, the infection site was CNS rather than respiratory system. Second, the causative organism was S. pneumoniae, which is rare cause of CNS infection in immunocompromised patients.

원문 : 야외운동기구를 이용한 운동프로그램이 노인들의 활동체력, 대사증후군 위험인자 및 염증인자에 미치는 영향

양상훈 ( Sang Hoon Yang ),김종식 ( Jong Shik Kim ) 한국운동생리학회(구 한국운동과학회) 2014 운동과학 Vol.23 No.3

양상훈, 김종식. 야외운동기구를 이용한 운동프로그램이 노인들의 활동체력, 대사증후군 위험인자 및 염증인자에 미치는 영향. 운동과학, 제23권 제3호, 229-240, 2014. 본 연구는 여성노인 65세 이상 16명을 대상으로 8주간의 야외운동기구를 이용한 운동프로그램 참여가 활동체력, 대사증후군 위험인자 및 염증인자에 미치는 영향을 규명하였다. 활동체력의 경우 악력은 유의한 차이가 없었으나 하지근력과 유연성은 운동 후 유의하게 증가한 것으로 나타났다. 대사증후군 위험인자의 경우 HDL-C는 운동 후 유의하게 증가하는 것으로 나타났으며, glucose는 유의하게 감소하는 것으로 나타났다. 허리둘레, 수축기 혈압, 이완기 혈압, TG는 유의한 차이가 없었다. 염증인자의 경우 CRP, TNF-α 모두 운동 후 감소하는 것으로 나타났으나 유의한 차이는 없었다. 결론적으로 야외운동기구 운동프로그램은 하지근력, 유연성 강화와 HDL-C 증가, glucose 감소의 연구결과를 보았을 때 더 다양한 야외운동기구를 이용한 운동프로그램을 개발하고 지속적으로 운동의 효율을 높여간다면 노화를 지연시킬 뿐만 아니라 각종 질병을 방지하고 건강을 유지시키는 효과를 기대할 수 있을 것으로 사료된다. Yang, Sang-Hoon, Kim, Jong-Shik. The Effects of Exercise Program Outdoor Exercise Equipment on Activity Fitness, Metabolic Syndrome Risk Factors and Inflammatory Factors in the Elderly. Exercise Science, 23(3): 229-240. 2014. This study is for observing the influence of exercising in outdoor exercise facilities for 8 weeks on activity fitness, risk factors of insulin resistance syndrome and inflammatory factors in 16 females who are aged 65 years or older. The major results on this analysis are as follows: In parameters for activity fitness, there was no significant difference in grasping power but a significant increase in strength of lower limb muscles and flexibility after exercise. For risk factors of insul in resistance syndrome, there was a significant increase in HDL-C after exercise with a significant decrease in gl ucose. There was no significant difference in waist l ine, systol ic bl ood pressure, diastol ic bl ood pressure and TG. For inflammatory factors, there was a decrease in CRP and TNF-α after exercise with no significance. In conclusion, the results showing positive effects on physical fitness of lower limb muscles and physiological markers including HDL-C and glucose indicates that elderly women exercising in outdoor exercise facilities may have benefits of antiaging effect, prevent various diseases and stay healthy if efficiency of exercise using outdoor exercise facilities is continually improved in a variety of training programs.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 이트라코나졸 액과 플루코나졸 시럽의 예방 효과 : 전향적, 무작위, 비교 임상시험

최수미,이동건,최정현,박선희,엄기성,김유진,김희제,민창기,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2005 감염과 화학요법 Vol.37 No.2

목적 : 조혈모세포이식 환자에서 호중구 감소 기간 동안 이트라코나졸 액(ITZS)과 플루코나졸 시럽(FCZS)의 진균 감염에 대한 예방 효과를 전향적으로 비교해 보고자 하였다. 재료 및 방법 : 2001년 8월부터 2002년 6월까지 가톨릭 조혈모세포이식센터에 입원하여 동종 조혈모세포이식을 받은 만 18세 이상의 환자들을 각각 ITZS와 FCZS군으로 무작위 배정하여 침습성 진균 감염 발생과 약제 관련 부작용을 전향적으로 조사하였다. 결과 : ITZS군에 40명, FCZS군에 38명이 배정되었고, FCZS군 환자의 평균 나이가 더 많았다. 시험 약제 평균투여기간은 ITZS군 16.4일, FCZS군 21.9일로 ITZS군이 더 짧았다. 이는 ITZS군에서 소화기 부작용으로 인한 약제 중단이 더 많았기 때문으로 보인다. 시험 약제 투여 후 총 빌리루빈 수치의 유의한 증가가 관찰되었으나, 약제 관련 부작용은 모든 환자에서 가역적이었다. 호중구감소 기간동안 표재성 진균 감염은 두 군에서 모두 발생하지 않았고, 침습성 진균 감염은 ITZS군 5명(12.5%), FCZS군 8명(21.1%)으로 FCZS군에서 많았으나, 통계학적으로 유의하지 않았고, 사망률에도 유의한 차이는 없었다. 결론 : 침습성 진균 감염에 대한 이트라코나졸 액과플루코라졸 시럽의 예방 효과에 유의한 차이는 없었으나, 앞으로 더 많은 환자를 대상으로 잘 짝지어진 대조군 연구가 필요할 것으로 보인다. 부작용 중 간독성과 관련하여 이식시 투여되는 여러 약제들과의 상호작용에 대한 연구도 필요할 것이다. Background : Though fluconazole is widely used for antifungal prophylaxis, it is ineffective against mould infections including Aspergillus species. Itraconazole has a broader spectrum than fluconazole but the capsule form shows erratic bioavailability in neutropenic patients. In this study, we compared itraconazole oral solution (ITZS) with fluconazole syrup (FCZS) for the prevention of invasive fungal infection (IFI) in allogeneic hematopoietic stem cell transplant recipients. Materials and Methods : Adults receiving allogeneic hematopoietic stem cell transplantation (HSCT) from september 2001 to June 2002, were randomly allocated to either the ITZS group or the FCZS group. We prospectively evaluated the safety and efficacy of each drug. Results : Out of 78 patients (40 patients in the ITZS group and 38 patients in the FCZS group) who were eligible for this study, 37 patients completed the course of prophylaxis without any evidence of IFI. The mean duration of prophylaxis was 16.4 days for the ITZS group and 21.9 days for the FCZS group (P<0.006). Drug-related adverse events occurred in 28 patients (70.0%) and 19 patients (50.0%) in the ITZS group and the FCZS group, respectively. Common adverse events of ITZS were nausea, vomiting, and diarrhea. Drug-related reversible hepatotoxicity occurred in 4 patients in the ITZS group. There was a significant elevation of total bilirubin level in the ITZS group. The incidence of suspected IFI occurred in 5 patients (12.5%) who received ITZS, compared with 8 (21.1%) who received FCZS (P=0.372). There were no proven IFIs or superficial (oral/vaginal) fungal infections in both groups. Overall mortality was not different between the two groups (2.5% in the ITZS group versus 5.3% in the FCZS group, P=0.610). Conclusion : ITZS and FCZS showed similar protection against IFI during pre-engraftment period. Poor tolerability due to gastrointestinal troubles of ITZS might limit its success as prophylactic therapy. Well matched controlled study with large number of patients will be required in the future.

가역 열화학반응을 이용한 축열에 관한 연구(Ⅵ)-축열식 열교환기의 방열특성 : 축열식 열교환기의 방열특성 Heat Release Characteristics of a Regenerative Heat Exchanger

金宗植,李泳世 계명대학교 산업기술연구소 1991 産業技術硏究所 論文報告集 Vol.14 No.2

The calcium hydroxide/calcium oxide reversible thermochemical reaction cycle has the greatest practicability as one of the most promising chemical heat storage material . It is necessary to select the optimal type of heat recovery device using the reversible thermochemical reaction cycle of Ca(OH)₂/CaO and to improve the production process of Ca(OH)₂. The experimental apparatus is a packed bed shape which is suitable to optimal type of heat recovery device. The reaction characteristics of heat release process for the hydration of CaO can be used to improve the production process of Ca(OH)₂.

화물터미널 건립의 경제적 분석에 관한 연구 : 익산시(춘포면) 만경강 하천부지를 중심으로

김중식,배종길 조선대학교 경영경제연구소 2002 經營經濟硏究 = Management and economics research Vol.25 No.1

Cyclohexanol-Phenol系의 活動度 係數 推定과 Wilson Parameter에 關한 硏究

金宗植 忠州大學校 1977 한국교통대학교 논문집 Vol.10 No.2

검수 수정 In this paper, selecting cyclohexanol - phenol system which is important in the process of caprolactum manufature, Wilson parameter was attempted to seek by surmising activity coefficient in vapor- biquid equilibrium data which has been published.

역삼투압을 이용한 전착도료 정제공장폐수처리에 관한 연구

김진성,김종식 啓明大學校 産業技術硏究所 1997 産業技術硏究所 論文報告集 Vol.20 No.-

To treat effectively EDIR (electrodeposition ion removal) wastewater in terms of COD 1,500∼2,000ppm generating from aluminum painting process, A RO(Reverse Osmosis) process was designed and installed to recover and reuse of solvent sent back to the electrocodeposition tank while the permeate reused as rinse water. A RO system in which 3 polyamide-spiral wound modules(102￠×1,016L) connected in series has been running to treat 20㎥ in waste volume in 3 days batch operation at the condition of system recovery 30%, applied pressure 11.5 kg/㎠ and room temperature. At 80% water recovery, rejections were suitable to allow reuse of permeate with stable flux 390 l/㎡-hr and average COD 300ppm for washing remained paint solution in ion-exchange tower instead of demineral water. COD rejection as a function of running time was showed in the range of 78∼87% at the condition of 42 hours-cycle batch operation and 5-fold decrease in waste volume and the observed solvent rejections for ethyl cellusolves, buthyl cellusolve and n-butanol were 79%, 87% and 70% respectively.