Antiproliferative and Antitumorigenic Activity of Toona sinensis Leaf Extracts in Lung Adenocarcinoma

Chih-Jen Yang,Yu-Jung Huang,Cheng-Yuan Wang,Chuan-Sheng Wang,Pei-Hui Wang,Jen-Yu Hung,Tung-Heng Wang,Hseng-Kuang Hsu,Hurng-Wern Huang,S.P. Anand Kumar,Ming-Shyan Huang,Ching-Feng Weng 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.1

Toona sinensis is a traditional Chinese herb, and the extracts of T. sinensis leaf possess a variety of biological functions. This study attempted to test the antiproliferative effect of TSL-1 (a bioactive fraction of T. sinensis) in H441 cells (lung adenocarcinoma). The data showed that the antiproliferative effect of TSL-1 on H441 cells is prominent using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. TSL-1-induced apoptosis was confirmed by cell morphology, sub-G1 peak accumulation, cleavage of poly(ADP)-ribose polymerase, and propidium iodide-annexin V double staining. Furthermore, decreased Bcl-2 accompanied by increased Bax (in western blotting) was found with TSL-1 treatment of H441 cells. TSL-1 treatment-induced G1 arrest was concurrent with the down-regulation of protein levels of cyclin D1 and cyclin-dependent kinase 4 in H441 cells. Peroral and intraperitoneal administrations of TSL-1 were performed to evaluate the therapeutic efficacy, and peroral administration of TSL-1 was also used to elucidate the therapeutic efficacy in the H441 cell xenograft model in vivo. The data revealed that TSL-1 treatment inhibited H441 tumor growth in both therapeutic and preventive experiments. Taken together, these results demonstrate that TSL-1 possesses the capability of preventing and alleviating lung cancer proliferation in vitro and in vivo with proven nephrological and hepatic safety and has the potential to be developed as an anti–lung cancer drug.

Pyogenic Liver Abscess as a Warning Sign for Primary Liver Cancer: A Nationwide Population-based Study

Huang, Wen-Kuan,Lin, Yung-Chang,Chiou, Meng-Jiun,Yang, Tsai-Sheng,Chang, John Wen-Cheng,Yu, Kuang-Hui,Kuo, Chang-Fu,See, Lai-Chu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Background: There have been no large-scale population-based studies to estimate the subsequent risk of primary liver cancer (PLC) among patients with pyogenic liver abscess (PLA). This study aimed to provide relevant data. Materials and Methods: The Taiwan Longitudinal Health Insurance Database for the years 2000 and 2005 was used. The PLA group were adult inpatients who were newly diagnosed with PLA from 2000 to 2008. The control group was randomly selected and matched with the PLA group in terms of age, sex, and date in which medical treatment was sought other than for PLA. Results: There were 1,987 patients each in the PLA and control groups. In total, 56 had PLC, 48 (2.4%, 601.5 per 100,000 person-years) from the PLA group, and 8 from the control group. After adjusting for potential covariates, the hazard ratio of PLC for the PLA group was 3.4 times that of the control group (95% confidence interval = 1.6-7.3, p <0.001). The PLC risk for the PLA group was significantly higher within the first year after PLA diagnosis (hazard ratio: 35.4) as compared with the control group and became insignificant (hazard ratio: 2.0, 95% confidence interval = 0.8-4.9) more than one year after PLA diagnosis. Conclusions: Patients with PLA have a higher rate of PLC than matched controls, especially within the first year after the diagnosis of PLA, suggesting PLA is a warning sign for PLC.

Transaxillary Capsulorrhaphy with Reimplantation to Correct Bottoming-Out Deformity in Breast Mycobacterial Periprosthetic Infection: A Case Report with Literature Review

Huang Tsung-Chun,Lee Jian-Jr,Yang Kuo-Hui,Chou Chia-Huei,Chang Yu-Chen 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.6

Augmentation mammoplasty is one of the most popular cosmetic surgeries, but there is a high reoperation rate (29.7%) commonly due to capsular contracture, implant malpositioning, infection, and unsatisfactory size. Although infection only accounts for 2% of cases, its management is very challenging, especially with nontuberculous mycobacteria (NTM) infection. Breast prosthetic NTM infection is a rare but is a disastrous condition with an incidence of approximately 0.013%. Immediate salvage reimplantation is usually not suggested, and most studies recommend a gap of 3 to 6 months after combination antibiotics therapy before reimplantation. However, delayed reimplantation often leads to great psychological stress and struggle between the doctor and patient. We present the case report of successful reimplantation in treating prosthetic NTM infections in a 28-year-old female. We discuss a novel technique “transaxillary capsulorrhaphy” to correct the bottoming-out deformity. One year after the combination of antibiotics and surgery, the follow-up computed tomography scan showed complete remission of NTM without recurrence. We discuss the surgical technique in detail. The 1-year follow-up assessment (photos and dynamic video) revealed good cosmesis and reliable correction using the new technique. This report is the first formal description and discussion of one-stage reimplantation following NTM infections. Transaxillary capsulorrhaphy allows for a successful salvage operation when an implant is displaced. This approach provides highly favorable result in eastern women undergoing revision augmentation mammoplasty. This study reflects level of evidence V, considering opinions of respected authorities based on clinical experience, descriptive studies, or reports of expert committees.

Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway

Huang Jun-Hao,Gao Hui-Wen,Gao Dong-Dong,Yang Wei-Yue,Zhao Meng-Ke,Shen Bing,Hu Min 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.3

Purpose: Increased evidence has shown that aerobic exercise reduces airway hyperresponsiveness in asthmatic individuals. However, the underlying mechanisms of action remain elusive. This study aimed to investigate the effect of exercise on airway smooth muscle (ASM) contractile function in asthmatic rats, and uncover the possible involvement of interleukin 4 (IL-4) and the store-operated Ca2+ entry (SOCE) pathway. Methods: In this study, chicken ovalbumin was used to induce asthma in male Sprague-Dawley rats. The exercise group received moderate-intensity aerobic exercise training for 4 weeks. IL-4 concentrations in bronchoalveolar lavage fluid (BALF) samples were evaluated by enzyme linked immunosorbent assay. The contractile function of the ASM was investigated using tracheal ring tension experiments and intracellular Ca2+ imaging techniques. Western blot analysis was used to evaluate expression levels of calcium-release activated calcium (CRAC) channel protein (Orai) and stromal interaction molecule 1 (STIM1) in ASM. Results: Our data showed that the carbachol-stimulated, SOCE-mediated contraction of rat ASM was significantly increased in asthmatic rats, which could be abolished by exercise. Pharmacological studies revealed that GSK5498A and BTP-2, selective blockers of CRAC channels significantly inhibited SOCE-induced ASM contraction. In addition, exercise inhibited the up-regulation of IL-4 in BALF as well as STIM1 and Orai expression in the ASM of asthmatic rats. In line with these observations, we demonstrated that pretreatment of the ASM with IL-4 up-regulated the expression level of STIM1, Orai1 and Orai2, thereby promoting SOCE-mediated ASM contraction. Conclusions: The data in this study reveal that aerobic exercise may improve the ASM contractile function in asthmatic rats by inhibiting IL-4 secretion and by down-regulating the expression of STIM1, Orai1 and Orai2, thus decreasing excessive SOCE-mediated ASM contraction in asthmatic rats.

Perceptual Quality Driven Frame-Rate Selection (PQD-FRS) for High-Frame-Rate Video

Huang, Qin,Jeong, Se Yoon,Yang, Shanglin,Zhang, Dichen,Hu, Sudeng,Kim, Hui Yong,Choi, Jin Soo,Kuo, C.-C. Jay IEEE 2016 IEEE transactions on broadcasting Vol.62 No.3

<P>Video of higher frame rates (HFR) reduces the visual artifact in large screen display at the cost of a higher coding bit rate (or transmission bandwidth). In this work, we propose a perceptual quality driven frame rate selection (PQD-FRS) method that assigns a time-varying frame rate to a sequence so as to reduce its transmission cost. The objective of the PQD-FRS method is to offer perceptually indistinguishable experience for a certain percentage of viewers. We first conduct a subjective test to characterize the relationship between human perceived quality and video contents, and build a frame-rate-dependent video quality assessment dataset to serve as the ground truth. Then, we use a machine learning approach for the design of the key module of the PQD-FRS method, called the 'satisfied user ratio (SUR) prediction.' The SUR prediction module predicts the percentage of satisfied viewers, who cannot differentiate video quality of a lower and HFR, using the support vector regression. It is confirmed by experimental results that the proposed SUR module can offer a so highly accurate prediction that the PQD-FRS system can dynamically assign a proper frame rate to video without any perceptual quality degradation for a majority of viewers.</P>

Hydration of acetone in the gas phase and in water solvent

Yang, Kiyull,Hsieh, Yih-Huang,Kim, Chan-Kyung,Zhang, Hui,Wolfe, Saul Canadian Science Publishing 2010 Canadian journal of chemistry Vol.88 No.1

<P> In water solvent, the hydration of acetone proceeds by a cyclic (cooperative) process in which concurrent C-O bond formation and proton transfer to oxygen take place through a solvent and (or) catalyst bridge. Reactivity is determined primarily by the concentration of a reactant complex and not the barrier from this complex. This situation is reversed in the gas phase; although the concentrations of reactive complexes are much higher than in solution, the barriers are also higher and dominant in determining reactivity. Calculations of isotope effects suggest that multiple hydron transfers are synchronous in the gas phase to avoid zwitterionic transition states. In solution, such transition states are stabilized by solvation and hydron transfers can be asynchronous. </P>

In situ control of nanowire resistance with real time monitoring by using self-heating induced oxidation

Zong Yang,Huang Hui,Zhao Danna,Tang Zhenan 한국물리학회 2020 Current Applied Physics Vol.20 No.7

It was firstly demonstrated that the resistance of bridging GaN nanowires (NWs) can be in situ controlled via current driven self-heating. Owning to the absence of contact barrier at the electrodes of bridging NWs, the Joule-heating can be generated mainly on the NW itself rather than on the electrodes. With increase of NW biasvoltage (BiV) from 2.5 V to 10 V, the generated Joule-heating can make the NW oxidized in air, which leads to about 700 fold increase in NW resistance (from 82.5 Ω to 6 × 104 Ω). Theoretical simulation indicated that a NW temperature of 649 K can realized with a BiV of 4 V, which agrees well with the observation of thermal emission microscope. Moreover, the measured oxygen composition in the NWs was increased with increasing BiV, which indicates that the NWs were oxidized by BiV induced self-heating. This work provides a simple method for precise control of NW resistance, which can be further applied to the formation of core/shell NWs with real time monitoring.

Lossless VQ Indices Compression Based on the High Correlation of Adjacent Image Blocks

( Zhi-hui Wang ),( Hai-rui Yang ),( Chin-chen Chang ),( Gwoboa Horng ),( Ying-hsuan Huang ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.8

Traditional vector quantization (VQ) schemes encode image blocks as VQ indices, in which there is significant similarity between the image block and the codeword of the VQ index. Thus, the method can compress an image and maintain good image quality. This paper proposes a novel lossless VQ indices compression algorithm to further compress the VQ index table. Our scheme exploits the high correlation of adjacent image blocks to search for the same VQ index with the current encoding index from the neighboring indices. To increase compression efficiency, codewords in the codebook are sorted according to the degree of similarity of adjacent VQ indices to generate a state codebook to find the same index with the current encoding index. Note that the repetition indices both on the search path and in the state codebooks are excluded to increase the possibility for matching the current encoding index. Experimental results illustrated the superiority of our scheme over other compression schemes in the index domain.

Analysis and experiments of a novel and compact 3-DOF precision positioning platform

Hu Huang,Hongwei Zhao,Zunqiang Fan,Hui Zhang,Zhichao Ma,Zhaojun Yang 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.11

A novel 3-DOF precision positioning platform with dimensions of 48 mm×50 mm×35 mm was designed by integrating piezo actuators and flexure hinges. The platform has a compact structure but it can do high precision positioning in three axes. The dynamic model of the platform in a single direction was established. Stiffness of the flexure hinges and modal characteristics of the flexure hinge mechanism were analyzed by the finite element method. Output displacements of the platform along three axes were forecasted via stiffness analysis. Output performance of the platform in x and y axes with open-loop control as well as the z-axis with closed-loop control was tested and discussed. The preliminary application of the platform in the field of nanoindentation indicates that the designed platform works well during nanoindentation tests, and the closed-loop control ensures the linear displacement output. With suitable control, the platform has the potential to realize different positioning functions under various working conditions.

Novel Low-Temperature-Active Phytase from Erwinia carotovora var. carotovota ACCC 10276

Huo Qing Huang,Hui Ying Luo,Ya Ru Wang,Da Wei Fu,Na Shao,Pei Long Yang,Kun Meng,Bin Yao 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.10