Soluble Expression of Recombinant Human Smp30 for Detecting Serum Smp30 Antibody Levels in Hepatocellular Carcinoma Patients

Zhang, Sheng-Chang,Huang, Peng,Zhao, Yong-Xiang,Liu, Shu-Yan,He, Shu-Jia,Xie, Xiao-Xun,Luo, Gou-Rong,Zhou, Su-Fang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Senescence marker protein 30 (SMP30), a hepatocellular carcinoma (HCC) associated antigen, was earlier shown by our research group to be highly expressed in HCC paracancerous tissues, but have low levels in HCC tissues. In order to detect anti-SMP30 antibody in serum of HCC patients, we established pET30a-SMP30 and pColdIII-SMP30 expression systems in Escherichia coli. However, the expression product was mainly in the form of inclusion bodies. In this research, we used several combinations of chaperones, four molecular chaperone plasmids with pET30a-SMP30 and five molecular chaperone plasmids with pColdIII-SMP30 to increase the amount of soluble protein. Results showed that co-expression of HIS-SMP30 with pTf16, combined with the addition of osmosis-regulator, and a two-step expression resulted in the highest enhancement of solubility. A total of 175 cases of HCC serum were studied by ELISA to detect anti-SMP30 antibody with recombinant SMP30 protein. Some 22 were positive and x2 two-sided tests all showed P>0.05, although it remained unclear whether there was a relationship between positive cases and clinical diagnostic data.

Efficacy and Safety of Endoscopic Resection Therapies for Rectal Carcinoid Tumors: A Meta-Analysis

Lei He,Tao Deng,He-Sheng Luo 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.1

Purpose: Several endoscopic resection therapies have been applied for the treatment of rectal carcinoid tumors. However, there is currently no consensus regarding the optimal strategy. We performed a meta-analysis to compare the efficacy and safety of endoscopic mucosal resection (EMR) or modified EMR (m-EMR) versus endoscopicsubmucosal dissection (ESD) for the treatment of rectal carcinoid tumors. Materials and Methods: PubMed, Web of Science, Medline, Embase and CNKI were searched up to the end of January 2014 in order to identify all studies on the effectsof EMR (or m-EMR) and ESD on rectal carcinoid tumors. Results: A total of fourteen studies involving 782 patients were included. The pooled data suggested a significantly higher rate of pathological complete resection among patients treated with ESD or m-EMR than those treated with EMR [odds ratio (OR)=0.42, 95% confidenceinterval (CI): 0.25‒0.71; OR=0.10, 95% CI: 0.03‒0.33, respectively], while there was no significant difference between the m-EMR group and ESD group (OR=1.19, 95% CI: 0.49‒2.86); The procedure time of ESD was longer than EMR or m-EMR groups [mean differences (MD)=-11.29, 95% CI: -14.19 ‒ -8.38, MD= -10.90, 95% CI: -18.69 ‒ -3.11, respectively], but it was insignificance between the EMR and m-EMR groups. No significant differences were detected among the treatmentgroups with regard to complications or recurrence. Conclusion: The results of this meta-analysis suggest that treatment of rectal carcinoid tumors with ESD or m-EMR is superior to EMR, and the efficacy of m-EMR is equivalence to ESD treatment. However, more well-designed studies are needed to confirm these findings.

Effect of Media Components and Morphology of Bacillus natto on Menaquinone-7 Synthesis in Submerged Fermentation

Miao-miao Luo,Lu-jing Ren,Sheng-lan Chen,Xiao-jun Ji,He Huang 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.6

Vitamin K2 (menaquinone or MK) plays an important role in blood clotting, cardiovascular disease, and anti-osteoporosis. A novel bacterial strain was isolated and identified as Bacillus natto based on 16SrDNA sequencing and LC-MS analysis. The objective of this study was to improve the extraction efficiency and productivity of MK-7 from B. natto. Acid-heating method efficiently disrupted B. natto cells for MK-7 extraction. Bacillus natto had a wide range of pH (5.0 ~ 9.0) for optimal growth. Its MK-7 yield was increased when rotation speed was increased to 200 rpm. The highest MK-7 yield was obtained when glycerol and soy peptone were used in the growth media. Batch fermentation was subsequently tested in 5 L bioreactor, which gave a high productivity of MK-7 (at 0.60 mg/L/h). A positive correlation between MK-7 yield and sporulation ratio was also found. This study provides valuable information on the extraction and production of menaquinone-7 from B. natto under submerged fermentation condition.

miR-148b-3p, miR-190b, and miR-429 Regulate Cell Progression and Act as Potential Biomarkers for Breast Cancer

Wenzhu Dai,Jixiang He,Ling Zheng,Mingyu Bi,Fei Hu,Minju Chen,Heng Niu,Jingyu Yang,Ying Luo,Wenru Tang,Miaomiao Sheng 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2

Purpose: Breast cancer is the most frequently diagnosed malignancy in women worldwide. MicroRNAs (miRNAs) are thought to serve as potential biomarkers in various cancers, including breast cancer. Methods: We evaluated the miRNA expression profiles in 1,083 breast cancer samples and 104 normal breast tissues from The Cancer Genome Atlas database. We used the edgeR package of R software to analyze the differentially expressed miRNAs in normal and cancer tissues, and screened survival-related miRNAs by Kaplan-Meier analysis. A receiver operating characteristic curve was generated to evaluate the accuracy of these miRNAs as molecular markers for breast cancer diagnosis. Furthermore, the functional role of these miRNAs was verified using cell experiments. Targets of candidate miRNAs were predicted using 9 online databases, and Gene Ontology (GO) functional annotation and pathway analyses were conducted using Database for Annotation, Visualization and Integrated Discovery online tool. Results: A total of 68 miRNAs showed significantly different expression patterns between the groups (p < 0.001), and 13 of these miRNAs were significantly associated with poor survival (p < 0.05). Three miRNAs with high specificity and sensitivity, namely, miR-148b-3p, miR-190b, and miR-429, were selected. In vitro experiments showed that the overexpression of these 3 miRNAs significantly promoted the proliferation and migration of MDA-MB-468 and T47D cells and reduced the apoptosis of T47D cells. GO and pathway enrichment analyses revealed that the targets of these dysregulated miRNAs were involved in many critical cancer-related biological processes and pathways. Conclusion: The miR-148b-3p, miR-190b, and miR-429 may serve as potential diagnostic and prognostic markers for breast cancer. This study demonstrated the roles of these 3 miRNAs in the initiation and progression of breast cancer.

Effects of Trimebutine Maleate on Colonic Motility through Ca^2+- activated K^+ Channels and L-type Ca^2+ Channels

Wei Tan,Hong Zhang,He-Sheng Luo,Hong Xia 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.6

The effects of trimebutine maleate (TM) on spontaneous contractions of colonic longitudinal muscle were investigated in guinea pigs. The contractile responses of smooth muscle strips were recorded by an isometric force transducer. Membrane and action potentials were detected by an intracellular microelectrode technique. The whole-cell patch clamp recording technique was used to record the changes in large conductance Ca^2+-activated K^+ (BKca) and L-type Ca^2+ currents in colonic smooth muscle cells. At high concentrations (30, 100, and 300 μM), TM inhibited the amplitude of spontaneous contractions. At low concentrations (1 and 10 μM), TM attenuated the frequency and tone of smooth muscle strips, whereas TM had no influence on the amplitude of spontaneous contractions. TM depolarized the membrane potentials, but decreased the amplitude and frequency of action potentials at high concentrations. TM inhibited BKca and L-type Ca^2+ currents in a dose-dependent manner. In the presence of the BKca channel opener, NS1619, TM also inhibited BKca currents. Bayk8644, a L-type Ca^2+channel opener, increased L-type Ca^2+ currents. This augmentation was also attenuated by TM. These results suggest that TM attenuates intestinal motility through inhibition of L-type Ca^2+ currents, and depolarizes membrane potentials by reducing BKca currents. Thus, TM may be a multiple-ion channel regulator in the gastrointestinal tract.

ORiginal Article : Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome

( Wen Jing Liang ),( Guo Zhang ),( He Sheng Luo ),( Lie Xin Liang ),( Dan Huang ),( Fa Can Zhang ) 대한간학회 2016 Gut and Liver Vol.10 No.3

Background/Aims: Previous studies have revealed that mast cells (MCs) may activate the protease-activated receptors and release of neuropeptides involved in the pathogenesis of irritable bowel syndrome (IBS). The levels of proteaseactivated receptor 2 (PAR-2) and tryptase can contribute to understanding the pathogenesis of IBS. Methods: Colonoscopic biopsies were performed of 38 subjects (20 with IBSdiarrhea [IBS-D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue staining. Results: Significant increases in the mRNA expression of tryptase (p<0.05, IBS-D, IBS-C vs control) and PAR-2 (p<0.05, IBS-D, IBS-C vs control) and in the tryptase protein level (p<0.05, IBS-D, IBS-C vs control) were detected in IBS. Elevations of MCs, CGRP, VIP and SP (p<0.05, IBS-D vs control) were observed for IBS-D only. Conclusions: Tryptase levels may upregulate the function of PAR- 2, resulting in the release of neuropeptide and they were correlated with clinical symptoms associated with IBS. (Gut Liver 2016;10:382-390)

Hot Deformation Characteristics and Processing Map Analysis of Pre-Forged AZ80 Magnesium Alloy

Shi‑quan Huang,Ming Lu,Sheng‑lan Luo,Hai‑lin He,You‑ping Yi 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.5

The hot deformation behavior of pre-forged AZ80 magnesium alloy is investigated by the isothermal compression tests attemperatures of 523&amp;amp;ndash;683 K and strain rates of 0.0001&amp;amp;ndash;0.1 s&amp;amp;minus;1, and analyzed by the processing maps for guiding isothermaldie forging. Flow localization and even cracking occurs at low temperatures and high strain rates, where shear deformationdegree shows a positive correlation with the &amp;amp;#55349;&amp;amp;#57097;( &amp;amp;#775;&amp;amp;#55349;&amp;amp;#57088; ) value. Two stability regions with high efficiency is found out by theprocessing maps. At common stability region with high temperatures and low strain rates, peak power dissipation efficiencydoes not represent optimum deformation condition as reported in other materials. A Z parameter criterion is introduced forparameters optimization. javascript:void(0); a new stability domain of 523&amp;amp;ndash;590 K and 0.0001&amp;amp;ndash;0.01 s&amp;amp;minus;1 is observed, which istypical for fine microstructure composed of equiaxed clean &amp;amp;alpha;-Mg grains and big particles both about 1 &amp;amp;mu;m (573 K/0.01 s&amp;amp;minus;1).Super plasticity is speculated to occur at that condition.

Genomic Sequence Analysis and Organization of BmKαTx11 and BmKαTx15 from Buthus martensii Karsch: Molecular Evolution of α-toxin genes

Xiu Ling Xu,Zhi Jian Cao,Ji Qun Sheng,Wen Lan Wu,Feng Luo,Yong Gang Sha,Xin Mao,Hui Liu,Da He Jiang,Wen Xin Li 한국생활환경학회 2005 BMB Reports Vol.38 No.4