Distinctions Between Clinicopathological Factors and Prognosis of Alpha-fetoprotein Negative and Positive Hepatocelluar Carcinoma Patients

Xu, Jia,Liu, Chang,Zhou, Lei,Tian, Feng,Tai, Ming-Hui,Wei, Ji-Chao,Qu, Kai,Meng, Fan-Di,Zhang, Ling-Qiang,Wang, Zhi-Xin,Zhang, Jing-Yao,Chang, Hu-Lin,Liu, Si-Nan,Xu, Xin-Shen,Song, Yan-Zhou,Liu, Jun,Z Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

Serum alpha-fetoprotein (AFP) is a significant marker for clinical diagnosis and prognosis evaluation in hepatocellular carcinoma (HCC) patients. However, some proportion of liver cancer patients are AFP-negative (AFP ${\leq}$20ng/ml). In order to study the differences between clinicopathological factors and prognosis of alpha-fetoprotein negative and positive patients, a total of 114 cases (41 AFP-negative and 73 AFP-positive) were selected for our research. By systematically statistical analysis, the results demonstrated that compared with AFP-negative patients, AFP-positive examples were more likely to feature cirrhosis nodules, non-complete neoplasm capsules, and a poor Edmondson-steiner grade. Furthermore, AFP-negative patients demonstrated a favorable long-term prognosis. By univariate analysis and multivariate analysis with Cox's proportional hazards model, multiple tumors were found to be independent risk factors for worse survival of AFP negative patients; however, less tumor-free margins, multiple tumors and Edmondson-steiner grades III/IV, proved to be independent risk factors leading to a poor prognosis of AFP positive cases. Finally, we can infer that high levels of AFP signify a highly malignant tumor and unfavorable prognosis.

Efficiently targeted therapy of glioblastoma xenograft via multifunctional biomimetic nanodrugs

Zhipeng Yao,Xiaochun Jiang,Hong Yao,Yafeng Wu,Fan Zhang,Cheng Wang,Chenxue Qi,Chenhui Zhao,Zeyu Wu,Min Qi,Jia Zhang,Xiaoxiang Cao,Zhichun Wang,Fei Wu,Chengyun Yao,Songqin Liu,Shizhang Ling,Hongping Xi 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: Glioblastoma multiforme (GBM) is a fatal malignant primary brain tumor in adults. The therapeutic efficacy of chemotherapeutic drugs is limited due to the blood-brain barrier (BBB), poor drug targeting, and short biological half-lives. Multifunctional biomimetic nanodrugs have great potential to overcome these limitations of chemotherapeutic drugs. Methods: We synthesized and characterized a biomimetic nanodrug CMS/PEG-DOX-M. The CMS/PEG-DOX-M effectively and rapidly released DOX in U87 MG cells. Cell proliferation and apoptosis assays were examined by the MTT and TUNEL assays. The penetration of nanodrugs through the BBB and anti-tumor efficacy were investigated in the orthotopic glioblastoma xenograft models. Results: We showed that CMS/PEG-DOX-M inhibited cell proliferation of U87 MG cells and effectively induced cell apoptosis of U87 MG cells. Intracranial antitumor experiments showed that free DOX hardly penetrated the BBB, but CMS/PEG-DOX-M effectively reached the orthotopic ntracranial tumor through the BBB and significantly inhibited tumor growth. Immunofluorescence staining of orthotopic tumor tissue sections confirmed that nanodrugs promoted apoptosis of tumor cells. This study developed a multimodal nanodrug treatment system with the enhanced abilities of tumor-targeting, BBB penetration, and cancer-specific accumulation of chemotherapeutic drugs by combining chemotherapy and photothermal therapy. It can be used as a flexible and effective GBM treatment system and it may also be used for the treatment of other central nervous systems (CNS) tumors and extracranial tumors.

Tribological Properties of PAO40@SiO2/UHMWPE Composites Subjected to Heavy-loading Conditions

Yao Chenyu,Jia Dan,Wan Changxin,Li Jian,Zhan Shengpeng,Yang Tian,Qi Xiaowen,Liu Changxin,Duan Haitao 한국고분자학회 2022 폴리머 Vol.46 No.5

In the present study, the ballistic impact resistance of kevlar reinforced polymer composites and shape memoryalloy sheet reinforced kevlar epoxy polymer composites have been compared numerically and experimentally. The testspecimens have been fabricated by the hand layup method, and the ballistic impact test has been conducted accordingto NIJ Level IIIA. In the current research, a new method has been proposed to measure the ballistic impact resistanceof the material by using Image J software. From the test results, it has been observed that reinforcement of shape memoryalloy in kevlar epoxy polymer composites has increased the ballistic impact resistance by 89-145% for the damaged areacompared with plain kevlar reinforced epoxy polymer composite. Also, the damaged area observed in the numerical andthe experimental results is almost the same.

Betulin Targets Lipin1/2-Meidated P2X7 Receptor as a Therapeutic Approach to Attenuate Lipid Accumulation and Metaflammation

( Jia-yi Dou ),( Yu-chen Jiang ),( Zhong-he Hu ),( Kun-chen Yao ),( Ming-hui Yuan ),( Xiao-xue Bao ),( Mei-jie Zhou ),( Yue Liu ),( Zhao-xu Li ),( Li-hua Lian ),( Ji-xing Nan ),( Yan-ling Wu ) 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.3

The present study focused on the potential mechanism of betulin (BT), a pentacyclic triterpenoid isolated from the bark of white birch (Betula pubescens), against chronic alcohol-induced lipid accumulation and metaflammation. AML-12 and RAW 264.7 cells were administered ethanol (EtOH), lipopolysaccharide (LPS) or BT. Male C57BL/6 mice were fed Lieber-DeCarli liquid diets containing 5% EtOH for 4 weeks, followed by single EtOH gavage on the last day and simultaneous treatment with BT (20 or 50 mg/kg) by oral gavage once per day. In vitro, MTT showed that 0-25 mM EtOH and 0-25 μM BT had no toxic effect on AML-12 cells. BT could regulate sterolregulatory-element-binding protein 1 (SREBP1), lipin1/2, P2X7 receptor (P2X7r) and NOD-like receptor family, pyrin domains-containing protein 3 (NLRP3) expressions again EtOH-stimulation. Oil Red O staining also indicated that BT significantly reduced lipid accumulation in EtOH-stimulated AML-12 cells. Lipin1/2 deficiency indicated that BT might mediate lipin1/2 to regulate SREBP1 and P2X7r expression and further alleviate lipid accumulation and inflammation. In vivo, BT significantly alleviated histopathological changes, reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and triglyceride (TG) levels, and regulated lipin1/2, SREBP1, peroxisome proliferator activated receptor α/γ (PPARα/γ) and PGC-1α expression compared with the EtOH group. BT reduced the secretion of inflammatory factors and blocked the P2X7r-NLRP3 signaling pathway. Collectively, BT attenuated lipid accumulation and metaflammation by regulating the lipin1/2-mediated P2X7r signaling pathway.

A Three-Dimensional ZnO/CdS/NiFe Layered Double Hydroxide Photoanode Coupled with a Cu2O Photocathode in a Tandem Cell for Overall Solar Water Splitting

Jia Liu,Yinghua Zhang,Zhiming Bai,Zhian Huang,Yukun Gao,Yuan Yao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.11

An integrated tandem photoelectrochemical (PEC) cell, composed of a three-dimensional (3D) ZnO/CdS/NiFe layered double hydroxide (LDH) core/shell/hierarchical nanowire arrays (NWAs) photoanode and a p-Cu2O photocathode, was designed for unassisted overall solar water splitting in this study. The optical and photoelectrochemical characteristics of ZnO-based photoanodes and Cu2O photocathode were investigated. The results show that ZnO/CdS/NiFe LDH nanostructures offer significantly enhanced performances with a photocurrent density reaching 5.8 mA · cm -2 at 0.9 V and an onset potential as early as 0.1 V (versus RHE). The enhancement can be attributed to the existence of CdS nanoparticles (NPs) which boosts the light absorption in visible region and enhances charge separation. Moreover, the introduction of NiFe LDH nanoplates, with unique hierarchical mesoporous architecture, promotes electrochemical reactions by providing more active sites as co-catalyst. On the above basis, the ZnO/CdS/NiFe LDH–Cu2O two-electrode tandem cell system was established. At zero bias, the device shows a photocurrent density of 0.4 mA · cm -2 along with the corresponding solar-to-hydrogen (STH) conversion efficiency reaching 0.50%. Our results indicate that the tandem PEC cells consisting of metal–oxide–semiconductor photoelectrodes based on Earth-abundant and low-cost materials hold promising application potential for overall solar water splitting.

The comparison of microbial communities in thyroid tissues from thyroid carcinoma patients

Liu Chen-Jian,Chen Si-Qian,Zhang Si-Yao,Wang Jia-Lun,Tang Xiao-Dan,Yang Kun-Xian,Li Xiao-Ran 한국미생물학회 2021 The journal of microbiology Vol.59 No.11

Thyroid carcinoma is a common endocrine organ cancer associated with abnormal hormone secretion, leading to the disorder of metabolism. The intestinal microbiota is vital to maintain digestive and immunologic homeostasis. The relevant information of the microbial community in the gut and thyroid, including composition, structure, and relationship, is unclear in thyroid carcinoma patients. A total of 93 samples from 25 patients were included in this study. The results showed that microbial communities existed in thyroid tissue; gut and thyroid had high abundance of facultative anaerobes from the Proteobacteria phyla. The microbial metabolism from the thyroid and gut may be affected by the thyroid carcinoma cells. The cooccurrence network showed that the margins of different thyroid tissues were unique areas with more competition; the stabilization of microcommunities from tissue and stool may be maintained by several clusters of species that may execute different vital metabolism processes dominantly that are attributed to the microenvironment of cancer.

Effect of Run Ze Tonic in Mice with Kidney-Yin Deficiency

Shuo Liu,Si-yao Ma,Wen-yi Zhu,Jia Liang,Hong Meng 한국피부과학연구원 2021 아시안뷰티화장품학술지 Vol.19 No.1

목적: 본 연구는 Run Ze Tonic의 신장-음 결핍 마우스의 갑상선 호르몬, 부신 기능, 성 호르몬 및 주기적 뉴클레오티드에 미치는 영향을 관찰하고자 한다. 방법: ICR 마우스를 정상 대조군, 모델 그룹, Run Ze Tonic 고용량 그룹, 중간 용량 그룹 및 저용량 그룹으로 분류하였으며, 매 그룹은 20마리씩 구성하였다. 신장-음 결핍 마우스 모델은 연속 10 일 동안 1 일 1 회 레보 티록신 나트륨을 위내 직접 투여에 의해 확립되었다. 각 그룹의 마우스의 일반특징 및 체중을 관찰하고 장기지수를 측정하였다. 그리고 ELISA 방법으로 테트라 요오 도티 로닌 (T4), 트리 요오 도티 로닌 (T3), 갑상선 호르몬 (TSH), 코르티코 트로 핀 방출 호르몬 (CRH), 부 신피질 자극 호르몬 (ACTH), 코르티 코스 테론 (CORT), 테스토스테론 (T), 에스트라 디올 (E2), 고리 형 아데노신 모노 포스페이트 (cAMP) , 사 이 클릭 구아노 신 모노 포스페이트 (cGMP)를 측정하였다. 결과: Run Ze Tonic 고용량군은 신장-음결핍 마우스의 일반적인 신체 징후를 개선하고, 체중을 증가시키고(p<0.05), TSH 함량을 증가시킨다(p<0.01). 고용량 군에서 T3 및 T4 수치가 유의하게 감소하 였으며(p<0.01), ACTH 수준도 유의하게 감소하였다(p<0.05). 신장 음 결핍 암컷 마우스는 고용량 군에서 성 호르몬 E2 및 E2/T 비 율이 유의하게 감소(p<0.01)하였다. 신장 음이 결핍 된 수컷 마우스의 경우 고용량 군에서 성 호르몬 T함량이 유의하게 증가되었다 (p<0.05). 그리고 고용량 군에서 고리 형 뉴클레오티드 시스템의 cAMP 수치가 감소하였다(p<0.01). 결론: Run Ze Tonic은 내분비 계에서 호르몬 수치 장애, 부신 피질 기능 및 에너지 대사 균형을 조절하여 음양을 키우고 신장을 강화하는 역할을 할 수 있다고 사료된다. Purpose: To observe the effects of Run Ze Tonic on thyroid hormone, adrenal function, sex hormones and cyclic nucleotide of mice with kidney-yin deficiency. Methods: ICR mice were randomly grouped into normal control group, model group, Run Ze Tonic high dose group, the middle dose group and low dose group, male and female in half. The kidney-yin deficiency mice models were established by intragastric administration of levothyroxine sodium before intragastric administration of corresponding drugs once a day for 10 consecutive days. One day after the last administration, the effects were observed by measuring weight. And tetraiodothyronine (T4), triiodothyronine (T3), thyrotropin (TSH), corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT), testosterone (T), estradiol (E2), cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) were tested by ELISA method. Results: Compared with the model group, the body weight, TSH level increased significantly (p <0.01, p<0.05), and T3, T4, ACTH level decreased significantly in high dose group (p <0.01, p <0.05). The E2 level in female decreased significantly in high dose group (p <0.01), the same as the ratio of E2/T (p <0.01). The T level in male increased significantly in high dose group (p <0.01), the same as the ratio of E2/T (p <0.01). And cAMP level decreased in the high dose group(p <0.05). Conclusion: Run Ze Tonic may play the role of nourishing yin and strengthening kidney by adjusting hormone level disorder, adrenal cortex function and balancing energy metabolism in endocrine system.

FADD Phosphorylation Modulates Blood Glucose Levels by Decreasing the Expression of InsulinDegrading Enzyme

Lin, Yan,Liu, Jia,Chen, Jia,Yao, Chun,Yang, Yunwen,Wang, Jie,Zhuang, Hongqin,Hua, Zi-Chun Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.4

Our previous study revealed a novel role of Fas-associated death domain-containing protein (FADD) in islet development and insulin secretion. Insulin-degrading enzyme (IDE) is a zinc metalloprotease that selectively degrades biologically important substrates associated with type 2 diabetes (T2DM). The current study was designed to investigate the effect of FADD phosphorylation on IDE. We found that the mRNA and protein levels of IDE were significantly downregulated in FADD-D mouse livers compared with control mice. Quantitative real-time polymerase chain reaction analysis showed that FADD regulates the expression of IDE at the transcriptional level without affecting the stability of the mRNA in HepG2 cells. Following treatment with cycloheximide, the IDE protein degradation rate was found to be increased in both FADD-D primary hepatocytes and FADD-knockdown HepG2 cells. Additionally, IDE expression levels were reduced in insulin-stimulated primary hepatocytes from FADD-D mice compared to those from control mice. Moreover, FADD phosphorylation promotes nuclear translocation of FoxO1, thus inhibiting the transcriptional activity of the IDE promoter. Together, these findings imply a novel role of FADD in the reduction of protein stability and expression levels of IDE.

Ultrasound Utility for Predicting Biological Behavior of Invasive Ductal Breast Cancers

Zhang, Lei,Liu, Yu-Jie,Jiang, Shuang-Quan,Cui, Hao,Li, Zi-Yao,Tian, Jia-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Purpose: The aim of the study was to evaluate the correlation of ultrasound features with breast cancer molecular status. Materials and Methods: A retrospective review was performed of ultrasound findings in 263 patients diagnosed with breast invasive ductal carcinoma for comparison with immunohistochemistric results were obtained from each lesion. Relationships between ultrasound findings and molecular status were investigated by using multiple regression analysis by means of stepwise logistic regression. Differences in ultrasound criteria were assessed among women with different molecular status. Results: ER positivity was associated with small size, lobulate, angular or spiculated margin contours, absence of calcification, posterior tumor shadowing and low elasticity score; PR positivity was associated with small size, lobulate or angular or spiculated margin contours and absence of calcification; HER2 positivity was associated with presence of calcification and absence of any echogenic halo. The calculated models of predicted molecular status were accurate and discriminating with AUCs of 0.78, 0.74, and 0.74, respectively. Conclusions: Breast cnacer ultrasound features show some correlation with the molecular status. These models may help to expand the scope of ultrasound in predicting tumor biology.

Name and Maintain Topological Faces in Rotating and Scanning Features

Gao Xue-Yao,Li Jia-Qi,Guo Hao,Gao Yun-Feng,Liu Yu-Hong 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.3

Features created in rotating and scanning operations are very complex. Naming and identifying their topological faces is an important problem in CAD fields. In this paper, a new method of coding topological faces in rotating and scanning features is proposed. Firstly, contour segments are numbered. Secondly, an angle between contour segment and rotating axis is computed. Thirdly, all topological faces are named based on contour segments’ numbers, rotating axis and other information. When a face splits and several subfaces merge, a method of processing their codes is given. The proposed method is applied to HUST-CAID feature modeling system. Experimental results show that it can name and identify topological faces effectively in operations.