Changes in αB-crystallin, tubulin, and MHC isoforms by hindlimb unloading show different expression patterns in various hindlimb muscles

( Hyun Seok Jee ),( Taka Shi Skurai ),( Jae Young Lim ),( Hideo Hatta ) 한국운동영양학회 2014 Physical Activity and Nutrition (Phys Act Nutr) Vol.18 No.2

Hyun Seok Jee, Takashi Sakurai, Jae-Young Lim and Hideo Hatta. Changes in αB-crystallin, tubulin, and MHC isoforms by hindlimbunloading show different expression patterns in various hindlimb muscles. JENB., Vol. 18, No. 2, pp.161-168, 2014 [Purpose]αB-crystallin is a small heat shock protein that acts as a molecular chaperone under various stress conditions. Microtubules, whichconsist of tubulin, are related to maintain the intracellular organelles and cellular morphology. These two proteins have been shownto be related to the properties of different types of myofibers based on their contractile properties. The response of these proteinsduring muscular atrophy, which induces a myofibril component change, is not clearly understood. [Methods] We performed 15days of hindlimb unloading on rats to investigate the transitions of these proteins by analyzing their absolute quantities. Proteincontents were analyzed in the soleus, plantaris, and gastrocnemius muscles of the unloading and control groups (N = 6). [Results]All three muscles were significantly atrophied by hindlimb unloading (P < 0.01): soleus (47.5%), plantaris (16.3%), and gastrocnemius(21.3%) compared to each control group. αB-crystallin was significantly reduced in all three examined unloaded hindlimb musclescompared to controls (P < 0.01) during the transition of the myosin heavy chain to fast twitch muscles. α-Tubulin responded onlyin the unloaded soleus muscle. Muscle atrophy induced the reduction of αB-crystallin and α-tubulin expressions in plantar flexormuscles with a shift to the fast muscle fiber compared to the control. [Conclusion] The novel finding of this study is that bothproteins, αB-crystallin and α-tubulin, were downregulated in slow muscles (P < 0.01); However, α-tubulin was not significantly reduced compared to the control in fast muscles (P < 0.01). [Keyword] Microtubule, αB-crystallin, tubulin, myosin heavy chainisoform, skeletal muscle

관상동맥질환의 중증도와 혈중지질치 및 아포지단백과의 상관관계

임현주,황종현,류재근,정병천,조용근,채성철,전재은,박의현 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.1

목적 : 관상동맥질환의 위험인자로서 혈청지질 및 혈장 지단백에 대한 관심이 높아지고 있고 또 아포지단백이 관상동맥 질환을 일으키는데 다른 지질보다 더 중요하다는 연구가 많이 발표되고 있으나 우리나라에서는 아포지단백에 대한 연구가 그리 많지 않은 편이다. 이에 저자등은 혈중지질치와 새로운 위험인자로 알려지고 있는 아포지단백치를 측정하고 이들과 관상동맥질환 중증도와의 상관 관계를 알아보았다. 대상 및 방법 : 관상동맥질환이 의심되어 관상동맥조영술을 실시한 69명의 환자를 대상으로 하였으며 12시간 이상 공복후 혈청을 채취하여 총콜레스테롤, 중성지방, HDL-콜레스테롤, 아포지단백 AI (ape AI), 아포지단백 B (ape B)를 측정하였다. 관상동맥조영상 50%이상의 협착이 있는 혈관의 수 (lesion number), 최대협착을 합한 값 (lesion score), 각 분절의 최대협착을 합한 값 (total score), 가장 심한 협착 값 (peak stenosis) 등으로 관상동맥 중증도를 알아본 다음 이들과 혈청지질치, 아포지단백과의 상관 관계를 비교해 보았다. 상관 관계는 Spearman's correlation coefficient를 구하였다. 결과 : 관상동맥질환의 중증도와 각 지질치 간의 상관 관계를 비교해 보았을때 lesion number와 관계있는 것은 연령 (r=0.2789), 중성지방치 (r=0.2829)이며 lesion score와 관계있는 것은 연령(r=0.2911), 중성지방치0 (r=0.3316), 총콜레스테롤과 HDL-콜레스테롤의 비 (r=0.2361), apo B 치(r=0.2759), apo AI과 apo B의 비 (-0.3154)로 나타났다. Total score는 연령 (r=0.3100), 총콜레스테롤치 (r=0.2452), 중성지방치 (r=0.3022), 총콜레스테롤과 HDL-콜레스테롤의 비 (r=0.2770), apo B치 (r=0.2927), apo AI과 apo B의 비 (r=-0.3408)와 연관이 있었고 peak stenosis는 중성지방 치(r=0.3305), ape B 치 (r=0.2968), apo AI과 apo B의 비 (r=-0.3977)와 관계가 있었다. 연령으로 조정하여 partial correlation을 좌면, 관상동맥조영술상 중증도는 중성지방치 (r=0.3408), apo B 치(r=0.2877), apo AI과 apo B의 비 (r=-0.3460)와 유의한 상관 관계가 있었다. 이중 ape AI과 ape B의 비는 lesion score (r=-0.2640), total score (r=-0.3057), peak stenosis (r=-0.3460)와 유의한 상관관계를 보였다. 결론 : 이상의 결과로 보아 apo B치 그리고 apo AI과 apo B의 비가 관상동맥 중증도의 예견인자로 다른 혈중 지질 치보다 더 유용하다는 것을 알 수 있었다. Objectives : Recently, it was reported that the measurements of apolipoprotein levels may be valuable in the clinical assessment of coronary artery disease severity. However there are a few reports regarding to the relationship between coronary artery disease severity and apolipoprotein levels in Korea. Thus, we measured serum lipid and apolipoprotein levels and studied the relationship between coronary artery disease severity and serum lipids and apolipoproteins levels. Subjects and Methods : The 69 patients who underwent coronary angiography to evaluate chest pain were subjected to this study. We measured the levels of serum cholesterol, triglycerides, HDL cholesterol, apolipoprotein AI(apo AI) and apolipoprotein B(Apo B) and measured the severity of coronary artery disease by lesion number, lesion score, total score and peak stenosis. Then, we studied the relationship between coronary artery disease severity and apolipoproteins and calculated the correlation coefficient. Results : The results were as follows. There are significant correlation(r=0.3) between age, triglycerides, apo B levels, apo AI/B ratio and coronary artery disease severity. In controlling for age, tyiglycerides, apo B levels and apo AI/B ratio shows significant correlation(r=0.3) with coronary artery disease severity. Especially, apo AI/B ratio shows significant correlation(r=0.3) with lesion number, total score and peak stenosis. Conclusion : These results suggest that the apo B levels and the ratio of apo AI/B can be used as significant independent predictor for coronary artery disease severity rather than other serum lipid levels.

급성 심근허혈에 의해 윤발된 승모판 폐쇄부전의 기전에 관한 실험적 연구

강덕현,송현,송재관,김현숙,이재환,임희영,이재원,박성욱,박승정,송명근 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.8

급성하벽심근경색에서 전흉부유도 ST절 하강의 의의

정병천,배호상,곽동훈,김영태,배용학,황종현,임현주,류재근,조용근,채성철,전재은,박의현 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : 급성하벽심근경색증 환자에서 심전도상 흔히 동반되는 전흉부유도 ST절 하강의 의의를 알아보았다. 방법 : 심근경색후 24시간에서 48시간에 얻은 심전도를 기준으로 52명의 급성하벽심근경색증 환자를 전흉부유도에서 ST절의 하강이 있는 군과 없는 군으로 나누었으며 심도자, 관상동맥조영술, 심초음파도 및 방사성 핵종을 이용한 좌심구혈율 측정 등을 시행하였다. 결과 : 1) 좌심실확장기말압은 전흉부유도에서 ST절의 하강이 있는 경우에서 16.9±5.33mmHg로 없는 경우의 11.0±4.02mmHg보다 유의하게 높았다(p<0.05). 그러나 운동량 및 좌심구혈을은 흥부 유도상 ST절의 하강이 있는 경우 7.6±3.0 METs 및 53.9±9.1%로 없는 경우의 9.1±2.7 METs 및 54.6±9.5%보다 낮았으나 통계적 유의성은 없었다(p=0.180, p=0.823). 2) 관상동맥조영술상 전흉부 ST절의 하강이 동반된 군에서는 유의한 좌전하행지나 좌회선지의 협착이 있는 경우가 60.9% 및 57.9%로 ST절의 하강이 없는 군에서의 34.5% 및 7.7%와 비교해 좌관상동맥에 협착이 동반된 경우가 많았다(p=0.050, p=0.001). 3) 관상동맥조영술상 세 혈관질환의 빈도는 흉부유도상 ST절의 하강을 보인 23예에서 10예로 ST절 하강이 없었던 29예중 2예에 비하여 유의하게 많았고(P=0.007), 단 혈관질환의 빈도는 ST절의 하강을 보인 군에서 23예중 5예로 ST절 하강이 없었던 29예중 17예에 비하여 유의하게 적었다(P=0.002). 두 혈관질환의 경우에는 각각 8예와 10예로 양군간에 유의한 차이가 없었다. 4) 흉부유도상 ST절 하강의 빈도는 단 혈관질환 22예 중 5예에서, 두 혈관질환 18예중 8예, 세 혈관질환 12예 중 10예에서 관찰되어 협착이 있는 관상동맥의 수가 많을수록 ST절의 하강이 나타나는 빈도가 많음을 보였다(p=0.00308). 그러나 흉부유도상 나타난 ST절의 하강을 모두 합하여 구한 ST절 하강의 합은 협착이 있는 관상동맥의 수와 통계적인 유의성이 없었다. 5) 심초음파도상 좌심실 하벽의 벽운동은 ST절 하강을 보인 군에서는 akinesia가 83.3%, hypokinesia가 11.1%, 정상 벽운동이 5.6%로, ST절 하강이 없었던 군에서의 dyskinesia가 3.6%, akinesia가 39.3%, hypokinesia가 25.0%, 정상 벽운동이 32.1%와 비교하여 ST절의 하강이 있는 군에서 유의하게 벽운동의 장애가 빈번함을 보였다(p=0.03891). 그러나 좌심실전벽, 측벽 및 중격부위에서는 유의한 벽운동의 차이가 없었다. 결론 : 급성하벽심근경색증 환자의 전흉부유도에서 ST절의 하강이 동반되는 경우 다혈관 질환의 가능성이 높으며 좌심실확장기말압이 상승되었고 운동능력은 감소되어 있었다. 또한 심초음파도상 좌심실하벽의 벽운동장애도 심하였다. 따라서 전흉부 ST절의 하강을 동반한 급성하벽심근경색증 환자는 전흉부유도에 ST절 하강이 없는 경우보다 예후가 불량할 것으로 생각되며, 보다 적극적인 치료가 필요할 것으로 생각된다. The purpose of this study was to determine the significance of precordial ST segment depression appearing during acute inferior myocardial infarction and to assess the correlation of that with angiographic finding. 52 patients were allocated into two groups based on the existence of precordial ST segment depression : 23 patients with ST segment depression and 29 patients without ST segment depression. The extent of coronary artery disease as well as the prevalence of significant stenosis (≥70%) in left anterior descending artery were more frequent in patients showing precordial ST segment depression than in patients not showing them (p=0.050) and the result were similar in cases of left circumplex artery (p=0.001). On echocrdiographic examination of left ventricular (LV) wall motion, it showed more severely decreased inferior LV wall motion in patients showing precordial ST segment depression than in patients not showing them (p=0.03891) even though the other segment of LV wall motion didn't show the statistical difference between both groups. The other cardiac parameters such as LVEDP, exercise amount (METs) and ejection fraction were tend to showing bad profile in patients with precordial ST segment depression. From the above results, we could deduce that patients with precordial ST segment depression have relatively large infarction or concomitant left coronary artery disease, so they need more caution and intensive theraphy.

Sr·페라이트의 자기특성에 관한 연구

임재근,서강수,문현욱,신용진 明知大學校 産業技術硏究所 1992 産業技術硏究所論文集 Vol.11 No.-

This thesis is the study on the magnetic properties of strontium (Sr) ferrite. Specimens of SrO·nFe₂O₃,basic composition, with n=5.9 which is nonstoichiomatric composition region were prepared by sintering at 1275°C for 1hr, whthin air surroundings. In order to increase coercivity, additives of 0.4wt%SiO₂ and 0.7wt%CaCO₃ were added into basic composition, and 0.5wt%∼2.0wt%Cr₂O₃ was added as second additives. In case of second additives 1.5wt%Cr₂O₃, it was obtained uniformed grain size and magnetic properties was increased. The sintering temperature was 1150°C, 1180°C, 1210°C, 1240°C and 1270°C respectivily and cooling was performed in furnance. From the research and experiments on the magnetic properties of strontium (Sr) ferrite based on the complex additive technique, it was obtained good magnetic properties of (BH)mas=0.72MGOe, at the sintering temperature of 1210°C, in the case of (basic composition)+(basic additive)+(1.5wt%Cr₂O₃)

알루미늄-크롬 용융도금강판의 미세조직과 집합조직에 미치는 크롬의 영향

임병문,홍승현,정재인,최장현,정원섭 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.10

Microstructure and texture changes of aluminized coatings on steel sheets having Cr ranging from 0.0% to 1.0% in the coated layer were investigated. The surface morphology of the Al-Cr coated steel is not influenced by the increase of Cr contents in the coated layer. While the coated layer of aluminized steel consisted of two phases of Al and Al_(5)Fe₂, the aluminized coating layer containing Cr was composed of three phases of Al, Al_(13)Cr₂, and Al_(13)Fe₄+Al_(5)Fe₂. The surface layer was Al, the middle layer was Al_(13)Cr₂, and the interfacial layer between the coating and the steel substrate was Al_(13)Fe₄and Al_(5)Fe₂. The texture of the aluminized coating without Cr was characterized as a {001} fiber texture which major orientation was {001}<110>. As the Cr content in the aluminized layer was increased to 1.0%, the texture of the aluminized coating was not changed but the intensity of the {001} fiber texture was increased. The major orientation in the {001} fiber texture was changed from the rotated cube orientation of the aluminized coating with 0.1%Cr to the cube orientation of the aluminized coating with 1.0%Cr.

간편 간접추론방법 퍼지 제어기를 이용한 2×2 상호 결합공정 제어에 관한 연구

임재춘,채창현,고택범,류창렬 금오공과대학교 산업기술개발연구원 2000 産業技術開發硏究 Vol.16 No.-

This paper describes the design of SIIM (Simplified Indirect Inference Method) fuzzy controller and decoupling filter for 2x2 interacting process. The overall system for Two-Input Two-output(TITO) interacting processes consist of two fuzzy SISO PID controllers and 4 decoupling filter elements. The proposed fuzzy controller preserves the simple linear structure of its conventional counterpart, but has nonconstant gains, which enhances its self-tuning control capability, thereby significantly improves tracking control performance. The fuzzification, control-rule execution and defuzzification routines are so simple that the proposed controller has the ability of high speed inference and to adapt the increase of fuzzy inputs. The proposed fuzzy control system with three types decoupling filters are tested in tow types of 2x2 interaction processes. All computer simulation results have demonstrated superior to the control performance of the one proposed by K.A Toh et al.

多點 피토관 流量計의 流量係數 測定에 관한 실험적 연구

임재명,윤복현 木浦大學校 工業技術硏究所 2000 工業技術硏究誌 Vol.10 No.-

The performance of multi-point Pitot tube flow-meter with diamond shape depending on the combination of upstream double elbows was experimentally studied. Actual flow-rates were measured by using ISA 1932 nozzles which are all standard flow-meters. Based on the measured flow-rates the flow coefficients of the multi-point flow-meter could be obtained. The flow coefficients calculated with all the front four holes open agree very well with those calculated by means of the average coefficient for the all holes. Furthermore, the flow coefficients were quite indifferent to Reynolds number variation for the region of ReD> 5.5×104. It is mostly due to the fact that the straightener installed upstream makes the flow more stable and straight than the case without the straightener ahead of the test section.

노인 뇌경색 환자(70세 이상)의 임상적 특징에 관한 연구

임재환,박형국,성기범,신현길 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

Stroke is one of the most important diseases in the elderly, but studies on the elderly stroke(age ≥70 years) are rare. The authors evaluated clinical characteristics of ischemic stroke in the elderly and compared them with those in the young(age ≤69). The conclusion obtained are as follows. 1. The strokes with multiple risk factors and combined diseases were more common in the elderly than in the young. 2. Middle cerebral artery territory was most frequently involved in the two groups, but vertebrobasilar territory was more frequently involved in the elderly than in the young. 3. The incidence of recurrent stroke and pathogenesis did not differ significantly between the two groups. 4. Among the complications, urogenital diseases were more common in the elderly than in the young.

Uni-Class를 위한 컨텍스트 인식 시스템

임재현 公州大學校 工學硏究院 生産技術硏究所 2004 論文集 Vol.12 No.-

In this paper we propose a unified context-aware application model which is an essential part to develop various applications in ubiquitous computing environment. The proposed model affirms the independence between sensor and application by using unified context in the form of external context and internal context. It also ensures that application exploits relatively accurate context to trigger personalized services. To show usefulness of the proposed model, we apply it to the sensors and applications the ubi-class, a testbed for ubi-class system applications.