In Vitro Screening for Compounds Derived from Traditional Chinese Medicines with Antiviral Activities Against Porcine Reproductive and Respiratory Syndrome Virus

( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.

Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells

Jun-Cheng Guo,Yi-Jun Yang,Jin-Fang Zheng,Jian-Quan Zhang,Min Guo,Xiang Yang,Xiang-Ling Jiang,Li Xiang,You Li,Huang Ping,Liu Zhuo 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Hepatocellular carcinoma (HCC) is a major cause of cancer-related deaths, but its molecular mechanisms are not yet well characterized. Long noncoding RNAs (lncRNAs) play crucial roles in tumorigenesis, including that of HCC. However, the role of homeobox A11 antisense (HOXA11-AS) in determining HCC stem cell characteristics remains to be explained; hence, this study aimed to investigate the effects of HOXA11-AS on HCC stem cell characteristics. Initially, the expression patterns of HOXA11-AS and HOXA11 in HCC tissues, cells, and stem cells were determined. HCC stem cells, successfully sorted from Hep3B and Huh7 cells, were transfected with short hairpin or overexpression plasmids for HOXA11-AS or HOXA11 overexpression and depletion, with an aim to study the influences of these mediators on the self-renewal, proliferation, migration, and tumorigenicity of HCC stem cells in vivo. Additionally, the potential relationship and the regulatory mechanisms that link HOXA11-AS, HOXA11, and the Wnt signaling pathway were explored through treatment with Dickkopf-1 (a Wnt signaling pathway inhibitor). HCC stem cells showed high expression of HOXA11-AS and low expression of HOXA11. Both HOXA11-AS silencing and HOXA11 overexpression suppressed the self-renewal, proliferation, migration, and tumorigenicity of HCC stem cells in vivo, as evidenced by the decreased expression of cancer stem cell surface markers (CD133 and CD44) and stemness-related transcription factors (Nanog, Sox2, and Oct4). Moreover, silencing HOXA11-AS inactivated the Wnt signaling pathway by decreasing the methylation level of the HOXA11 promoter, thereby inhibiting HCC stem cell characteristics. Collectively, this study suggested that HOXA11-AS silencing exerts an antitumor effect, suppressing HCC development via Wnt signaling pathway inactivation by decreasing the methylation level of the HOXA11 promoter.

The Method of Predicting Average Response Time of Cloud Service Based on MGM (1, N) - BP Neural Network

Jun Guo,Qun Ma,Qingmin Ma,Yongming Yan,Qingliang Han 보안공학연구지원센터(IJGDC) 2014 International Journal of Grid and Distributed Comp Vol.7 No.2

Experimental research for the protective effect of Naoxingtong-containing serum on rat cerebral microvascular endothelial cells

Jun, Zhou,Jianyou, Guo,Jian, Guo,Lanfang, Li,Canghai, Li,Nan, Jiang,Shuying, Guo,Hairu, Huo,JiangTingliang, JiangTingliang Kyung Hee Oriental Medicine Research Center 2005 Oriental pharmacy and experimental medicine Vol.5 No.2

The protective effect of Naoxingtong (NXT) on rat cerebral microvascular endothelial cell (rCMEC) was investigated. rCMEC was injured in vitro by incubating for 4 hours at 100% NO in a hypoxia chamber. After treated with NXT-containing serum, the cellular viability rate (90.3%) was significantly elevated when compared with that of control group and the inhibitive rate of lactic dehydrogenase activity (9.2%) was far lower than the control group with dose-dependent effect. The results indicate that NXT can increase viability of rCMEC, and protect cell membrane from injury during hypoxia.

Control strategy for the substructuring testing systems to simulate soil-structure interaction

Jun Guo,Zhenyun Tang,Shicai Chen,Zhenbao Li 국제구조공학회 2016 Smart Structures and Systems, An International Jou Vol.18 No.6

Real-time substructuring techniques are currently an advanced experimental method for testing large size specimens in the laboratory. In dynamic substructuring, the whole tested system is split into two linked parts, the part of particular interest or nonlinearity, which is tested physically, and the remanding part which is tested numerically. To achieve near-perfect synchronization of the interface response between the physical specimen and the numerical model, a good controller is needed to compensate for transfer system dynamics, nonlinearities, uncertainties and time-varying parameters within the physical substructures. This paper presents the substructuring approach and control performance of the linear and the adaptive controllers for testing the dynamic characteristics of soil-structure-interaction system (SSI). This is difficult to emulate as an entire system in the laboratory because of the size and power supply limitations of the experimental facilities. A modified linear substructuring controller (MLSC) is proposed to replace the linear substructuring controller (LSC).The MLSC doesn\'t require the accurate mathematical model of the physical structure that is required by the LSC. The effects of parameter identification errors of physical structure and the shaking table on the control performance of the MLSC are analysed. An adaptive controller was designed to compensate for the errors from the simplification of the physical model in the MLSC, and from parameter identification errors. Comparative simulation and experimental tests were then performed to evaluate the performance of the MLSC and the adaptive controller.

The Influence of Market Concentration on Bank Performance and the Test of TBTF Behavior in China

Jun-guo Shi,Qi Xu,Xiaoxian Zhu 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.9

Exile Writer, Intellectualized Aesthetics and Obscure Art: Interpreting James Joyce and His Artistic Aspiration

Guo Jun 한국제임스조이스학회 2008 제임스조이스저널 Vol.14 No.2

Throughout his life, James Joyce is a self-exile both physically and spiritually. But that is not a mark of his escapism, isolationism or egoism. Instead, it is his strategy to transcend reification and to de-territorialize Ireland from its parochialism in his narration of his nation. This makes him a “nay-sayer” to the dominant national, cultural and religious discourses and a “heteroglossia” in representing his nation in literature among his contemporary compatriot writers. In doing so, his intention is to enlighten his countrymen and get his nation on its way towards spiritual liberation. In this sense, his works represent the very conscience of an intellectual-writer. Corresponding to his exile spirit is his intellectualized aesthetics in which “intellect” is not meant to differ from “imagination,” but is to be understood in Julien Banda’s sense to indicate stasis, transcendence, reason, pursuit of naked truth and justice in resistance against all kinds of blind passion and romanticism, partisan, racial or national. Based on his aesthetic principles, his artistic aspiration in all his works is to involve readers in intellectual reflections and polemics by subverting the conventional story writing techniques and by changing the readers’ automatic reading habit. The result is the creating of his obscure art, making it impossible for a “rapid skimming and absorption of the scant cream of sense” (in Beckett’s words) but confronting readers with the task of penetrating, analyzing, synthesizing and reconstruction. Thus, all his works have not only a unique artistic value but pedagogical significance in that they can train the readers to be rational beings and critical readers. Throughout his life, James Joyce is a self-exile both physically and spiritually. But that is not a mark of his escapism, isolationism or egoism. Instead, it is his strategy to transcend reification and to de-territorialize Ireland from its parochialism in his narration of his nation. This makes him a “nay-sayer” to the dominant national, cultural and religious discourses and a “heteroglossia” in representing his nation in literature among his contemporary compatriot writers. In doing so, his intention is to enlighten his countrymen and get his nation on its way towards spiritual liberation. In this sense, his works represent the very conscience of an intellectual-writer. Corresponding to his exile spirit is his intellectualized aesthetics in which “intellect” is not meant to differ from “imagination,” but is to be understood in Julien Banda’s sense to indicate stasis, transcendence, reason, pursuit of naked truth and justice in resistance against all kinds of blind passion and romanticism, partisan, racial or national. Based on his aesthetic principles, his artistic aspiration in all his works is to involve readers in intellectual reflections and polemics by subverting the conventional story writing techniques and by changing the readers’ automatic reading habit. The result is the creating of his obscure art, making it impossible for a “rapid skimming and absorption of the scant cream of sense” (in Beckett’s words) but confronting readers with the task of penetrating, analyzing, synthesizing and reconstruction. Thus, all his works have not only a unique artistic value but pedagogical significance in that they can train the readers to be rational beings and critical readers.

Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans

( Guo Jun Lang ),( Ming Yan Zhang ),( Bao Ling Li ),( Lin Lin Yu ),( Xing Meng Lu ),( Chuan Xi Zhang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.8

Several organophosphorus (OP) insecticides can selectively kill the silkworm maggot, Exorista sorbillans (Es) (Diptera: Tachinidae), while not obviously affecting the host (Bombyx mori) larvae, but the mechanism is not yet clear. In this study, the cDNA encoding an acetylcholinesterase (AChE) from the field Es was isolated. One point mutation (Gly353Ala) was identified. The Es-353G AChE and Es-353A AChE were expressed in baculovirus- insect cell system, respectively. The inhibition results showed that for eserine and Chlorpyrifos, Es-353A AChE was significantly less sensitive than Es-353G AChE. Meanwhile, comparison of the I(50) values of eserine, dichlorvos, Chlorpyrifos and omethoate of recombinant Es AChEs with its host (Bombyx mori) AChEs indicated that, both Es AChEs are more sensitive than B. mori AChEs. The results give an insight of the mechanism that some OP insecticides can selectively kills Es while without distinct effect on its host, B. mori. [BMB reports 2010; 43(8): 573-578]

Organization process of the hierarchical structures in microbially synthesized polyhydroxyalkanoates

Jun Xu,Bao-Hua Guo,Qiong Wu,Jin-Chun Chen,Guo-Qiang Chen,Jian-Jun Zhou,Yong Jiang,Lin Li 한국물리학회 2007 Current Applied Physics Vol.7 No.s1

cess of the high-order structures in biomaterials. Real-time Fourier transform infrared spectroscopy and optical microscopy demon-strated that helical segments formed along with the spherulite growth. Atomic force microscopy revealed the details of growth,twisting and branching of lamellar crystals. Cooperative packing of these twisting lamellae led to regular banded spherulites observedunder polarized light microscopy. Real-time observation on the crystallization process provided richer information than the characterization of the final structures; consequently, it provides deeper insight into the organization mechanism of the hierarchical structures.

A facile and reliable route to prepare highly dispersive ammonium dimolybdate uniform crystals from commercial molybdenum oxide

Jun Zhang,Yanhui Guo,Weiwei Lu,Xuming Guo,Mingchen Xu,Hongbo Guo 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.5

Highly dispersive ammonium dimolybdate crystals with high purity have been prepared through reaction–evaporation–crystallization united route using commercial molybdenum oxide as starting material. The as-prepared ammonium dimolybdate uniform crystals were systematically characterized by X-ray powder diffraction, transmission-reflection optical microscope, Fourier transform infrared spectroscopy and TG-DSC thermal analysis. The Fsss grain diameter, loose packed density, and crystal-grain size distribution were also measured by the Malvern laser particle sizer. These collective characterization and analysis reveal that the ammonium dimolybdate grains are non-agglomerated uniform crystals with well normal distribution and geometric shape. The average Fsss diameter, d(0.5) and loose packed density are 433.316 mm, 470.981 mm and 1.592 g cm3, respectively, which could well meet the quality requirements for the subsequent production of molybdenum powders.