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In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response
Guo, Wei,Li, Guo-Jun,Xu, Hong-Bo,Xie, Jie-Shi,Shi, Tai-Ping,Zhang, Sheng-Zhong,Chen, Xiao-Hong,Huang, Zhi-Gang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Background: Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes. Methods: Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro. Results: Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Conclusion: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo.
Ya-Jun Gong,Jin-Cui Chen,Shao-Kun Guo,Pan Shi,Li-Jun Cao,Ming-Liang Li,Ary A. Hoffmann,Shu-Jun Wei 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4
The fall webworm (FWW) Hyphantria cunea, native to North America, is a globally invasive pest of a wide range of forest and fruit trees. Spraying of pesticides is the primary method for the control of FWW. In this study, toxicity and feeding cessation of two potential pesticides against the FWW, chlorantraniliprole, and chromafenozide, were evaluated. Both pesticides were slow to affect FWW. For chlorantraniliprole, the highest mortality of third instar larvae occurred at 72 h with an LC 50 of 10.34 mg/L, while for chromafenozide, the highest mortality occurred at 72 h with an LC 50 value 74.0950 mg/L. Low concentrations of both pesticides led to larvae ceasing to feed after six hours (chlorantraniliprole) and 24 h (chromafenozide). Both pesticides had persistent effects; thirty days after being applied at concentrations of 16, 26.67, and 35.56 mg/L to leaves, 93.33% of newly contacted larvae died after seven days. Our study showed that chlorantraniliprole and chromafenozide could be alternatively used against FWW and form a component of integrated control programs. The results provide information to guide the usage of chlorantraniliprole and chromafenozide in FWW control.
Guo, Ya-Jie,Shi, Ze-Ming,Liu, Jun-Da,Lei, Ning,Chen, Qiu-Hong,Tang, Ying Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9
Background: Polymorphisms of the Taq I gene have been associated with prostate cancer risk. Methods:We applied a fixed-effects model to combine odds ratios (ORs) and 95% confidence intervals (95% CI). The Egger's test was carried out to evaluate potential publication bias. Results: A total of 10 case-control studies enrolling 1,141 prostate cancer patients and 1,685 controls were included in this meta-analysis. Compared with the T allele, the OR for the C allele was 0.81 (0.70-0.94). The ORs for CT and CC+CT genotypes were 0.86 (0.74-1.01) and 0.84 (0.73-0.97) compared to wide type genotype (homozygote TT). Conclusions: The present meta-analysis suggests that the TF gene Taq I polymorphism may reduce the prostate cancer risk in Asian populations.
Shi-Qian Liu,Zhong-Liang Jing,Jun-Guo Lu 제어·로봇·시스템학회 2010 International Journal of Control, Automation, and Vol.8 No.6
An output tracking control problem for an unmanned tandem rotor helicopter with variance constraints is investigated in this paper. A modified Trajectory Linearization Control (TLC) is proposed to stabilize a nonlinear continuous-time flight dynamics system of the tandem helicopter. The tracking controller structure of TLC is designed by using two-time-scale nonlinear dynamic inversion. The base control law of the translational and attitude loops is designed in a pseudo-inversion feedforward con-troller to deal with nonlinear features of the plant and a proportional integral controller to stabilize the linear slowly time-variant error system resulted from the nonlinear flight system. Furthermore, a feasi-ble TLC strategy is designed to meet a performance index set including steady trajectory tracking error variance and desired Parallel D-spectrum (PD-) eigenvalues to achieve good flight quality. The Vari-ance-constrained Trajectory Linearization Control (VCTLC) is designed to realize the desired steady tracking precision and agile capability. Flight simulation results show the VCTLC method is feasible and effective in attitude and altitude tracking.
Association Between EGF, TGF-β1 and TNF-α Gene Polymorphisms and Hepatocellular Carcinoma
Shi, Hai-Zhou,Ren, Peng,Lu, Qing-Jun,Niedrgethmnn, Marco,Wu, Guo-Yang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12
Introduction: Up to present, EGF $61^*A$/G, TGF-${\beta}1$-$509^*T$/C and TNF-${\alpha}$-$308^*A$/G gene polymorphisms have been analysed in other cancer entities than hepatocellular carcinoma (HCC). We here investigated the frequency of these gene polymorphisms among HCC patients. Materials and Methods: A total of 73 HCC patients and 117 cancer-free healthy people were recruited at the Surgical Department of Zhongshan Hospital. Genomic DNA was isolated from peripheral blood and gene polymorphisms were analyzed by PCR-RFLP. Results: The distribution of EGF $61^*G$/G homozygotes among HCC patients was more frequent than that in the control group (24.7% vs 11.1%, OR=2.618, 95%CI=1.195-5.738). In parallel, the frequency of the "G" allele in the HCC patient group was also higher than that in the control group (45.9% vs 33.3%, OR= 1.696, 95%CI=1.110-2.592). No difference could be found for the TGF-${\beta}1$-509 and TNF-${\alpha}$-308 genotypes. Conclusion: EGF $61^*G$/G genotype and G allele are significantly increased among patients with HCC. TGF-${\beta}1$-$509^*T$/C and TNF-${\alpha}$-$308^*A$/G gene polymorphisms are not related to this cancer entity.
Jun Tan,Ju Chu,Wenjuan Shi,Cheng Lin,Yuanxin Guo,Ying-ping Zhuang,Siliang Zhang,Tadayuki Imanaka 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6
Most of the fermentation experiment designs were limited by the low-throughput of shake flask, especially for the medium optimization. A simple high-throughput screening system was developed for the determination of pigment in Monascus purpureus fermentation samples. This downscaled system was designed to optimize medium composition combined with statistical methods. The total 29 experiments designed by the Box–Behnken were used to study the 4 most important operating variables on pigment production. The analysis revealed that the optimum concentrations of glucose, peptone, NaNO3, and KH2PO4were 51.42, 4.91, 1.00, and 1.00 g/L, respectively. A production of 69.5 U/mL was achieved in agreement with the prediction (68.9 U/mL) fermented in 24-deep-well microtiterplates. Furthermore, the fermentation medium optimized in the high-throughput system was verified in shake flasks, and the pigment production could be enhanced from 206.5 U/mL in un-optimized medium to 265.8 U/mL,giving nearly 1.30-fold increase in production.
Shi Guangli,Sun Dan,Wang Zhenxing,Liu Xiaoying,Guo Jianhui,Zhang Susu,Zhao Ying,Ai Jun 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.4
A high-density genetic map is a prerequisite for marker-assisted selection of crop species. Here, we developed a high-density genetic map of Vitis using 92 F1 progeny derived from the cross between two wine grape cultivars, ‘Shuanghong’ and ‘Italian Riesling’. Whole genome resequencing (WGS) technology was used to generate SNP markers for the map. The map consisted of 1351 recombination bin markers (210,873 SNPs), distributed on 19 linkage groups (LGs), with an average distance of 0.65 cM between adjacent bin markers and a map distance of 860.46 cM. The high levels of uniform coverage and collinearity between the 19 LGs and the grape reference genome indicated that the map is of high quality and can serve as a powerful platform for evaluating grapevines and deciphering the genetic mechanism of important economic and agronomic traits. Our findings will facilitate the process of mapping quantitative trait loci (QTL) that control important horticultural and economic traits of grape.
Cyclic Behavior of Sand under Traffic Loading with ‘Inclined’ Consolidation
Lin Guo,Yuan Fang,Tingyu Wu,Jun Wang,Hongxu Jin,Li Shi 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5
To investigate the vertical strain development of sand under traffic loading after the process of ‘inclined’ consolidation, a series of cyclic drained tests were carried out by using a hollow cylinder apparatus. Three parameters were considered in this paper: initial consolidation angle, vertical cyclic stress ratio and cyclic torsional stress ratio. Test results show that the vertical strain under traffic loading path is larger than that of specimens whose cyclic torsional stress ratio is equal to 0 in the same situation. Furthermore, the vertical stain will decrease with the increase of consolidating inclination in the situation of same values of vertical cyclic stress ratio and cyclic torsional stress ratio. When initial consolidation angle is equal to 0o and 22.5o, the vertical strain will increase with increasing value of cyclic torsional stress ratio, while the effect of cyclic torsional stress ratio on vertical strain will be less significant when initial consolidation angle is equal to 45o. Moreover the trend that vertical strain increases with increasing value of cyclic torsional stress ratio will be more significant for a larger value of vertical cyclic stress ratio.