Optimizing Preparation Conditions for Angiotensin-I-Converting Enzyme Inhibitory Peptides Derived from Enzymatic Hydrolysates of Ovalbumin

Qun Huang,Shu-gang Li,Hui Teng,Yong-guo Jin,Mei-hu Ma,Hong-bo Song 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

Angiotensin-I-converting enzyme (ACE) inhibitory peptides were prepared from ovalbumin using enzyme hydrolysis with pepsin as an enzyme source. Effects of pH, enzyme dosage, substrate concentration, hydrolysis temperature, and time on the degree of hydrolysis and the ACE inhibition rate were investigated using single factor experiments. Preparation conditions for ACE inhibitory peptides were optimized using a response surface design on the base of single factor experiments. Optimum preparation conditions were a substrate concentration of 5.2 g/100 mL of D.W with a pH value of 2.5, an enzyme dosage of 14,000 U/g, and a hydrolysis time of 250 min at 30℃. The ACE inhibition rate was up to 70.55±1.13% under these conditions.

Combined water flux enhancement of PES-based TFC membranes in ultrasonic-assisted forward osmosis processes

Qun Wang,Xueli Gao,Zhun Ma,Jian Wang,Xiaojuan Wang,Yang Yang,Congjie Gao 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.64 No.-

For PES-based TFC FO membranes, a significant water flux surge occurred after a moderate ultrasonic irradiation. The Change in membrane transport parameters illustrated that internal concentration polarization (ICP) was weakened due to decreased structural parameter. Combined water flux enhancement via real-time ultrasonic irradiation was obtained and related to the CP effect and membrane orientation. Increasing irradiation intensity was more suitable in PRO mode than in FO mode. Low FS concentration in concentration combinations represented high ratio of ultrasonic-induced flux increment to energy input. Multiplying concentration difference was beneficial to obtain high conversion ratio of ultrasonic power to water flux.

Polymorphysims of CYP17-I Gene in the Exons Were Associated with the Reproductive Endocrine of Japanese Flounder (Paralichthys olivaceus)

Ma, R.Q.,He, F.,Wen, H.S.,Li, J.F.,Mu, W.J.,Liu, M.,Zhang, Y.Q.,Hu, J.,Qun, L. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

The cytochrome P450c17-I (CYP17-I) is one of the enzymes critical to gonadal development and the synthesis of androgens. Two single nucleotide polymorphisms (SNPs) were detected within the coding region of the CYP17-I gene in a population of 75 male Japanese flounder (Paralichthys olivaceus). They were SNP1 (c.C445T) located in exon2 and SNP2 (c.T980C (p.Phe307Leu)) located in exon5. Four physiological indices, which were serum testosterone (T), serum $17{\beta}$-estradiol ($E_2$), Hepatosomatic index (HSI), and Gonadosomatic index (GSI), were studied to examine the effect of the two SNPs on the reproductive endocrines of Japanese flounder. Multiple comparisons revealed that CT genotype of SNP1 had a much lower T level than CC genotype (p<0.05) and the GSI of individuals with CC genotype of SNP2 was higher than those with TT genotype (p<0.05). Four diplotypes were constructed based on the two SNPs and the diplotype D3 had a significantly lower T level and GSI. In conclusion, the two SNPs were significantly associated with reproductive traits of Japanese flounder.

Simultaneous Determination of Tin, Nickel, Lead, Cadmium and Mercury in Cigarette Material by Solid Phase Extraction and HPLC

Hu, Qun,Yang, Guangyu,Ma, Jing,Liu, Jikai Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.10

A new method for the simultaneous determination of heavy metal ions in cigarette material by microwave digestion and reversed-phase high-performance liquid chromatography (RP-HPLC) has been developed. The cigarette material was digested by microwave digestion. Lead, cadmium, mercury, nickel and tin ions in the digested samples were pre-column derivatized with tetra-(2-chlorophenyl)-porphyrin ($T_2$-CPP) to form color chelates, which were then enriched by solid phase extraction with a $C_{18}$ cartridge. The chelates were separated on a Waters Xterra$^{TM}RP_{18}$ column by gradient elution with methanol (containing 0.05 mol/L pyrrolidine-aceticacid buffer salt, pH = 10.0) and acetone (containin0.05 mol/L pyrrolidine-acetic acid buffer salt, pH = 10.0)as mobile phase at a flow rate of 0.5mL/min and analyzed with a photodiode array detector from 350-600 nm. The detection limits of lead, cadmium, mercury, nickel and tin were 4,3,3,8 and 5 ng/L, respectively, in the original samples. This method was afforded good results.

Histone Deacetylase Inhibitor Trichostatin A Enhances Antitumor Effects of Docetaxel or Erlotinib in A549 Cell Line

Zhang, Qun-Cheng,Jiang, Shu-Juan,Zhang, Song,Ma, Xiao-Bin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Background and Objective: Histone deacetylase (HDAC) inhibitors represent a promising class of potential anticancer agents for treatment of human malignancies. In this study, we investigated the effect of trichostatin A (TSA), one such HDAC inhibitor, in combination with docetaxel (TXT), a cytotoxic chemotherapy agent or erlotinib, a novel molecular target therapy drug, on lung cancer A549 cells. Methods: A549 cells were treated with TXT, erlotinib alone or in combination with TSA, respectively. Cell viability, apoptosis, and cell cycle distribution were evaluated using MTT (3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide) assay, Hochst33258 staining and flow cytometry. Moreover, immunofluorescent staining and Western blot analysis were employed to examine alterations of ${\alpha}$-tubulin, heat shock protein 90 (hsp90), epidermal growth factor receptor (EGFR), and caspase-3 in response to the different exogenous stimuli. Results: Compared with single-agent treatment, co-treatment of A549 cells with TSA/TXT or TSA/erlotinib synergistically inhibited cell proliferation, induced apoptosis, and caused cell cycle delay at the $G_2/M$ transition. Treatment with TSA/TXT or TSA/erlotinib led to a significant increase of cleaved caspase-3 expression, also resulting in elevated acetylation of ${\alpha}$-tubulin or hsp90 and decreased expression of EGFR, which was negatively associated with the level of acetylated hsp90. Conclusions: Synergistic anti-tumor effects are observed between TXT or erlotinib and TSA on lung cancer cells. Such combinations may provide a more effective strategy for treating human lung cancer.

Exploring the oral microflora of preschool children

Wen Ren,Qun Zhang,Xuenan Liu,Shuguo Zheng,Lili Ma,Feng Chen,Tao Xu,Baohua Xu 한국미생물학회 2017 The journal of microbiology Vol.55 No.7

The oral cavity is one of the most important and complicated habitats in our body and supports diverse microbial communities. In this study, we aimed to determine the bacterial diversity and composition of various oral micro-niches. Samples were collected from supragingival plaque, saliva, and tongue coating from 10 preschool children (30 samples total). 16S rRNA gene pyrosequencing dataset generated 314,639 clean reads with an average of 10,488 ± 2,787 reads per sample. The phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Fusobacteria were predominant, accounting for more than 90% of the total sequences. We found the highest α diversity, microbial richness, and evenness in plaque, compared with saliva and tongue coating. Plaque was also distinguished from saliva and tongue coating by phylogenetic distances (weighted UniFrac). Taxa with different relative abundances were further identified, confirming the existence of microbial differences across the three niches. Core microbiomes were defined of each niche; however, only a small proportion of operational taxonomic units (8.07%) were shared by the three niches. Coaggregation between Actinomyces spp. and Streptococcus spp. and other correlations among periodontal pathogens, such as Prevotella, Fusobacteria, Capnocytophaga, and Tannerella, were shown by a co-occurrence network. In summary, our study provides a framework of oral microbial communities in the population of preschool children as a baseline for further studies of oral diseases related to microbes.

The Method of Predicting Average Response Time of Cloud Service Based on MGM (1, N) - BP Neural Network

Jun Guo,Qun Ma,Qingmin Ma,Yongming Yan,Qingliang Han 보안공학연구지원센터(IJGDC) 2014 International Journal of Grid and Distributed Comp Vol.7 No.2

The preparation of alginate–AgNPs composite fiber with green approach and its antibacterial activity

Xihui Zhao,Qun Li,Xiaomei Ma,Wanying Liu,Jianping Wang,Yanzhi Xia 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.24 No.-

Antibacterial alginate fibers containing silver nanoparticles (AgNPs) have been prepared in this work. Firstly, AgNPs colloid solution was obtained employing alginate as stabilizing agent and glucose as reducing agent. Then, sodium alginate powder was directly added to the above solution and the resultant sodium alginate–AgNPs spinning dope was spun to prepare alginate–AgNPs composite fibers. The fibers were characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction and thermal gravimetric analysis (TG). Mechanical properties of the fibers were also measured. The antimicrobial properties were investigated by Staphylococcus aureus and Escherichia coli, and the results indicated that the composite fibers showed strong antibacterial activity.

Supragingival Plaque Microbial Community Analysis of Children with Halitosis^s

( Wen Ren ),( Qun Zhang ),( Xuenan Liu ),( Shuguo Zheng ),( Lili Ma ),( Feng Chen ),( Tao Xu ),( Baohua Xu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12

As one of the most complex human-associated microbial habitats, the oral cavity harbors hundreds of bacteria. Halitosis is a prevalent oral condition that is typically caused by bacteria. The aim of this study was to analyze the microbial communities and predict functional profiles in supragingival plaque from healthy individuals and those with halitosis. Ten preschool children were enrolled in this study; five with halitosis and five without. Supragingival plaque was isolated from each participant and 16S rRNA gene pyrosequencing was used to identify the microbes present. Samples were primarily composed of Actinobacteria, Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, and Candidate phylum TM7. The α and β diversity indices did not differ between healthy and halitosis subjects. Fifteen operational taxonomic units (OTUs) were identified with significantly different relative abundances between healthy and halitosis plaques, and included the phylotypes of Prevotella sp., Leptotrichia sp., Actinomyces sp., Porphyromonas sp., Selenomonas sp., Selenomonas noxia, and Capnocytophaga ochracea. We suggest that these OTUs are candidate halitosis-associated pathogens. Functional profiles were predicted using PICRUSt, and nine level-3 KEGG Orthology groups were significantly different. Hub modules of co-occurrence networks implied that microbes in halitosis dental plaque were more highly conserved than microbes of healthy individuals` plaque. Collectively, our data provide a background for the oral microbiota associated with halitosis from supragingival plaque, and help explain the etiology of halitosis.

Complete mitochondrial genome of the meadow moth, Loxostege sticticalis (Lepidoptera: Pyraloidea: Crambidae), compared to other Pyraloidea moths

Hong-Fang Ma,Xi-Xi Zheng,Ming-Hui Peng,Hai-Xu Bian,Miao-Miao Chen,Yan-Qun Liu,Xing-Fu Jiang,Li Qin 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

The mitochondrial genome (mitogenome) is an important topic for comparative and evolutionary genomics, as well as phylogenetic and population genetics. However, there are limited data regarding the mitochondrial genome available of Pyraloidea, one of the largest superfamilies in Lepidoptera. In this report, we present the complete mitogenome of the meadow moth, Loxostege sticticalis L. (Lepidoptera: Pyraloidea: Crambidae), which is a serious economic pest of both crops and weedsworldwide, thereby enhancing the available genomic information for Pyraloidea. This circular genome is 15,218 bp in length, containing 13 protein-coding genes (PCGs), two rRNA genes (rRNAs), and 22 tRNA genes (tRNAs), with a typical gene orientation and order comparable to other sequenced Pyraloidea insects. The genome composition of the major strand exhibits highly AT bias (80.82%), with a slightly positive AT skew indicating the occurrence of more As than Ts. The L. sticticalis mitogenome has a total of 130 bp of intergenic spacer sequences spread over 15 regions, ranging in size from 1 to 48 bp, of which only two are common among the 23 total Pyraloidea moths that have data collected on the mitogenome (one is located between tRNAGln and ND2 with variation change in length and a limited sequence conservation, and the other is located between tRNASer(UCN) and ND1 with a conserved 6 bp motif ‘ATACTA’). The A + Trich region of 331 bp in the genome is comprised of non-repetitive sequences but contains an ATAGN motif followed by a poly-T stretch of 17 bp, a microsatellite-like (TA)11 element preceded by an ATTTA motif, and a poly-A stretch upstream tRNAMet. These conserved structures identified in the A + T-rich region are presented in all of the sequenced Pyraloidea species. We provide a mitogenome-based phylogeny of Pyraloidea species, in which L. sticticalis shares close ancestry to Ostrinia species with substantial evidence. Our phylogenetic analyses strongly divide Crambidae into two sister lineages, one consisting of Pyraustinae and Spilomelinae, while the other contains Crambinae, Acentropinae, Scopariinae, Schoenobiinae and Glaphyriinae. The mitogenome dataset also supports the basal split between Pyraustinae and Spilomelinae.