Enhanced ionic conductivity of composite solid electrolyte by directionally ordered structures of linear Li1.3Al0.3Ti1.7(PO4)3

You Li,Mulan Tang,Shuxin Xu,Shuchao Zhang,Yuxin Zhai,Jiarong Yin,Zhengguang Zou 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

The NASICON-type solid electrolyte structure of Li1.3Al0.3Ti1.7(PO4)3 (LATP) exhibits good electrochemicalperformance and thermal stability, and has been promising as a solid electrolyte. Here, a stable goodstabilityLATP precursor spinning solution was prepared using the sol–gel method for the first time. A linearLATP solid electrolyte with an oriented ordered structure was obtained using improved electrostaticspinning equipment. The sintering process regime of the LATP-ordered construction was determined. Theionic conductivity of the prepared LATP-PEO/LiClO4-PEG composite solid-state electrolyte with anordered structure was as high as 2.05 10-4 Scm1 at room temperature (25 C), one order of magnitudehigher than the ionic conductivity of the LATP composite solid-state electrolyte reported so far. Organicsolid-state electrolytes to protect LATP-ordered structured solid-state electrolytes yield excellent electrochemicalstability in lithium-metal batteries.

Abnormal differentiation of regulatory T cells and Th17 cells induced by perinatal bisphenol A exposure in female offspring mice

You‑dan Dong,Liang Gao,Feng‑juan Wu,Ren Lin,Yuan Meng,Li‑hong Jia,Xiao‑fei Wang 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2

Background Bisphenol A (BPA) is an environmental estrogen widely exposed to human beings, and there are more studies on its reproductive toxicity, endocrine disruption and neurobehavioral disorders. Recent few studies have found that BPA has immunotoxicity, and its mechanism is not clear. Therefore, the effects of BPA on immune system have attracted extensive attention. The aim of this study was to investigate the effect and mechanism of perinatal exposure to BPA on regulatory T cells (Treg) and Th17 cells in female offspring mice. Methods Twenty-one pregnant C57BL/6 mice were randomly divided into three groups: a control group, low-dose BPA (0.2 μg/mL) and high-dose BPA (2.0 μg/mL) exposure group. All received BPA exposure via drinking water from gestational day 6 to the end of lactation. Female offspring were fed a normal diet and drinking water for 1 month. The percentages of Treg and Th17 cells, the levels of Foxp3 and RORγt protein and IL-17 and TGF-β from spleen tissue or blood were measured in female offspring. Results The percentage of Treg cells and levels of Foxp3 protein decreased, while the percentage of Th17 cells and levels of RORγt protein increased, which showed a dose–effect relationship. The levels of serum TGF-β were significantly lower and the levels of serum IL-17 were statistically higher in BPA-exposed female offspring compared with controls (P < 0.05 or P < 0.01). But there were no statistical difference in the levels of serum TGF-β and IL-17 between 0.2 μg/mL and 2.0 μg/ mL BPA groups (P > 0.05). Conclusion BPA exposure during pregnancy and lactation could cause abnormal differentiation and function of Treg and Th17 cells in female offspring mice, which was associated with down-regulated Foxp3 and up-regulated RORγt protein, respectively. Our findings indicated that BPA exposure during early development may play an important role in the development of autoimmune diseases later.

Molecular structures, chemical properties and biological activities of polysaccharide from <i>Smilax glabra</i> rhizome

Lee, JeYoung,Li, Changsheng,Surayot, Utoomporn,Yelithao, Khamphone,Lee, SangMin,Park, WooJung,Tabarsa, Mehdi,You, SangGuan Elsevier 2018 International journal of biological macromolecules Vol.120 No.2

<P><B>Abstract</B></P> <P>The water-soluble crude polysaccharides, extracted from the rhizome of <I>Smilax glabra</I>, were fractionated using an anion exchange chromatography, yielding two fractions, F<SUB>1</SUB> and F<SUB>2</SUB>. The crude and fractions (F<SUB>1</SUB> and F<SUB>2</SUB>) mainly consisted of carbohydrates (66.7%–91.1%), proteins (7.30%–23.9%) and minor amount of sulfates (1.60%–9.40%). Glucose was the major monosaccharide unit of the polysaccharides with different levels of sugar constituents including galactose, arabinose, rhamnose and mannose. The molecular weight (M<SUB>w</SUB>) of crude and fractions ranged from 32,102–6.3 × 10<SUP>3</SUP> g/mol. The crude and fractions could stimulate RAW264.7 cells to release nitric oxide and cytokines via up-regulation of their mRNA expression by the activation of NF-κB and MAPKs pathways. The related pattern recognition receptors (PRRs) on the surface of the cells appeared to be TLR2 and CR3. The GC–MS analysis revealed that the main backbone of the most immune-enhancing F<SUB>2</SUB> was (1 → 4)-linked glucose and galactose chain with minor linkages of (1 → 6)-galactose, (1 → 3)-mannose, (1 → 2)-rhamnose and (1 → 5)-arabinose with some branches at C-3 and C-4 rhamnose, or C-6 galactose.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Polysaccharide was extracted and purified from <I>Smilax glabra</I> rhizome. </LI> <LI> Two fractions were obtained by DEAE Sepharose fast flow column fractionation. </LI> <LI> The molecular weight of crude and fractions ranged from 32,102 to 6.3 × 10<SUP>3</SUP> g/mol </LI> <LI> The <I>Smilax glabra</I> polysaccharide was appeared enhanced RAW264.7 cells activity. </LI> <LI> The most immune-enhancing F<SUB>2</SUB> polysaccharide mainly consisted of (1 → 4)-linked galactose </LI> </UL> </P>

Purification, structural analysis and mechanism of murine macrophage cell activation by sulfated polysaccharides from <i>Cystoseira indica</i>

Bahramzadeh, Saman,Tabarsa, Mehdi,You, SangGuan,Li, Changsheng,Bita, Seraj Elsevier 2019 Carbohydrate Polymers Vol.205 No.-

<P><B>Abstract</B></P> <P>Sulfated polysaccharides were isolated and purified from the water extract of <I>Cystoseira indica</I> using DEAE Sepharose Fast Flow column to evaluate their structure and macrophage stimulating capacity. Crude and fractionated polysaccharides, CIF<SUB>1</SUB> and CIF<SUB>2</SUB>, were mostly composed of neutral sugars (73.1%–78.6%) with relatively lower amounts of acidic sugars (1.3%–9.0%) and sulfate esters (6.9%–9.7%). The polymer chains of polysaccharides were mainly built of different levels of glucose (2.1%–30.8%), fucose (17.2%–24.4%), mannose (17.8%–20.6%) and galactose (16.7%–17.3%). The weight average molecular weight (<I>M</I> <SUB>w</SUB>) of polysaccharides varied between 573.1 × 10<SUP>3</SUP> g/mol to 1146.6 × 10<SUP>3</SUP> g/mol. The CIF<SUB>2</SUB> polysaccharide, as the most immunostimulating polysaccharide, remarkably induced the release of nitric oxide and inflammatory cytokines including TNF-α, IL-1β, IL-6 and IL-10 from RAW264.7 murine macrophage cells through NF-κB and PAMKs transduction signaling pathways via cell surface TLR4. The interconnections of sugars in CIF<SUB>2</SUB> polysaccharide were complex with (1→3)-fucopyranose, (1→2,3,4)-glucopyranose, (→1)-galactopyranose, (→1)-xylopyranose, (1→2)-rhamnopyranose and (1→2,3)-mannopyranose units being the most predominant residues.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sulfated polysaccharides were isolated from <I>Cystoseira indica.</I> </LI> <LI> Polysaccharides exerted high RAW264.7 macrophage stimulating activity. </LI> <LI> RAW264.7 cells were activated through NF-κB and MAPK signaling pathways. </LI> <LI> TLR4 was the major cell surface receptor interacting polysaccharides with RAW264.7 cells. </LI> <LI> The main backbone of the polysaccharides was consisted of (1→3)-fucopyranosyl units. </LI> </UL> </P>

Pyrophosphate-triggered nanoaggregates with aggregation-induced emission

Li, Chun-Tao,Xu, You-Liang,Yang, Jian-Gong,Chen, Yong,Kim, Hyeong Seok,Cao, Qian-Yong,Kim, Jong Seung Elsevier Sequoia 2017 Sensors and actuators. B Chemical Vol.251 No.-

<P><B>Abstract</B></P> <P>A novel tetraphenylethene-based probe bearing bis-imidazolium anion donors is herein reported for pyrophosphate anion recognition. This probe can self-assemble finite, small sphere nanoaggregates with very weak emission in aqueous solution, and changes into large rod-like nanoaggregates with strong aggregation-induced emission upon binding with the pyrophosphate anion.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A bis-imidazolium functionalized tetraphenylethene probe was prepared. </LI> <LI> This probe self-assemble finite small sphere nanoaggregates in aqueous solution. </LI> <LI> The probe can recognize pyrophosphate anion with strong aggregation-induced emission. </LI> <LI> The probe/pyrophosphate assembly can fluorescence assay alkaline phosphatase. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>A novel nanoaggregates for recognition of pyrophosphate anion with aggregation-induced emission in pure aqueous solution is introduced.</P> <P>[DISPLAY OMISSION]</P>

Pd core-shell alloy catalysts for high-temperature polymer electrolyte membrane fuel cells: Effect of the core composition on the activity towards oxygen reduction reactions

You, Dae Jong,Kim, Do Hyung,De Lile, Jeffrey Roshan,Li, Chengbin,Lee, Seung Geol,Kim, Ji Man,Pak, Chanho Elsevier 2018 Applied catalysis. A, General Vol.562 No.-

<P><B>Abstract</B></P> <P>Pd-based core-shell alloy-supported catalysts were prepared sequentially via a microwave-assisted polyol method and galvanic replacement. To investigate the effect of the core composition on the catalytic activity of such catalysts, three different Pd alloy cores (PdNi, PdCu, and PdNiCu) were prepared on carbon supports using a polyol method. Then, Pd and Ir were introduced simultaneously to form shells on the Pd alloy cores by galvanic replacement in aqueous solution, thereby producing catalysts designated as PdNi@PdIr/C, PdCu@PdIr/C, and PdNiCu@PdIr/C. X-ray diffraction revealed that all three catalysts exhibited the face-centered cubic structure of Pd without the presence of individual phases for Ni, Cu, and Ir. The core-shell structure of the Pd-based alloy nanoparticles on the carbon support was verified by the electron energy loss spectroscopy line profile of a 25 nm nanoparticle of PdNiCu@PdIr/C. Among the three Pd-based core-shell catalysts, the highest electrochemical surface area and oxygen reduction reaction (ORR) activity was observed for PdNiCu@PdIr/C. In addition, the membrane electrode assembly employing the PdNiCu@PdIr/C catalyst displayed a significantly improved voltage compared to the other two catalysts under high-temperature polymer electrolyte membrane fuel cell conditions at 150 °C. Single-cell durability tests conducted to measure the voltage change at a constant current density of 0.2 A cm<SUP>−2</SUP> showed a decay ratio of 12.3 μV h<SUP>−1</SUP>. These results suggest that the composition of the core in core-shell nanoparticles has an important influence on both the electronic properties in the Pd alloy core and compressive lattice strain on the PdIr shell. Control of these synergistic effects provides a new approach for developing catalysts with high ORR activity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three catalysts with PdNi, PdNiCu and PdCu cores and a PdIr shell were prepared by polyol method and galvanic replacement. </LI> <LI> A membrane electrode assembly with PdNiCu@PdIr/C catalyst showed the significantly improved performance and durability. </LI> <LI> The core component of core-shell catalysts has important role to improve the activity toward the oxygen reduction reaction. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Two-dimensional metal organic framework for effective gas absorption

Cheng, Kaiwu,Li, Yaojia,Gao, Zhiguo,Chen, Fanghui,You, Chaoqun,Sun, Baiwang Elsevier 2019 Inorganic Chemistry Communications Vol.101 No.-

<P><B>Abstract</B></P> <P>Herein, crystalline metal organic framework (MOF) nanosheets have been fabricated through using a modified soft physical exfoliation method from bulk crystals of a layered MOF, [Cd(4,4′‑bpy)<SUB>2</SUB>(H<SUB>2</SUB>O)<SUB>2</SUB>](ClO<SUB>4</SUB>)<SUB>2</SUB>·(2,4′‑bpy)<SUB>2</SUB>·H<SUB>2</SUB>O (MOF-1), and fully characterized via transmission electron microscope (TEM), scanning electron microscopy (SEM), and atomic force microscope (AFM). The delaminated MOF-1 nanosheets with porous structure showed good growth potential in selective gas adsorption.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Using a modified soft physical exfoliation method from bulk crystals, we firstly synthesized a layered MOF nanosheets. </LI> <LI> The MOF-2 nanosheets revealed a superior absorption performance. </LI> <LI> The delaminated MOF nanosheets with porous structure showed good growth potential in selective gas adsorption. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing

Li Shi-Dan,Xing Wei,Wang Shao-Chuan,Li You-Bin,Jiang Hao,Zheng Han-Xuan,Li Xiao-Ming,Yang Jing,Guo De-Bin,Xie Xiao-Yu,Jiang Ren-Qing,Fan Chao,Li Lei,Xu Xiang,Fei Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Bone fracture remains a common occurrence, with a population-weighted incidence of approximately 3.21 per 1000. In addition, approximately 2% to 50% of patients with skeletal fractures will develop an infection, one of the causes of disordered bone healing. Dysfunction of bone marrow mesenchymal stem cells (BMSCs) plays a key role in disordered bone repair. However, the specific mechanisms underlying BMSC dysfunction caused by bone infection are largely unknown. In this study, we discovered that Fibulin2 expression was upregulated in infected bone tissues and that BMSCs were the source of infection-induced Fibulin2. Importantly, Fibulin2 knockout accelerated mineralized bone formation during skeletal development and inhibited inflammatory bone resorption. We demonstrated that Fibulin2 suppressed BMSC osteogenic differentiation by binding to Notch2 and inactivating the Notch2 signaling pathway. Moreover, Fibulin2 knockdown restored Notch2 pathway activation and promoted BMSC osteogenesis; these outcomes were abolished by DAPT, a Notch inhibitor. Furthermore, transplanted Fibulin2 knockdown BMSCs displayed better bone repair potential in vivo. Altogether, Fibulin2 is a negative regulator of BMSC osteogenic differentiation that inhibits osteogenesis by inactivating the Notch2 signaling pathway in infected bone.

miR-98 suppresses melanoma metastasis through a negative feedback loop with its target gene IL-6

Fei Li,Xin-ji Li,Li Qiao,Fei Shi,Wen Liu,You Li,Yu-ping Dang,Weijie Gu,Xiao-gang Wang,Wei Liu 생화학분자생물학회 2014 Experimental and molecular medicine Vol.46 No.-

Dysregulated microRNA (miRNA) expression has a critical role in tumor development and metastasis. However, the mechanism by which miRNAs control melanoma metastasis is unknown. Here, we report reduced miR-98 expression in melanoma tissues with increasing tumor stage as well as metastasis; its expression is also negatively associated with melanoma patient survival. Furthermore, we demonstrate that miR-98 inhibits melanoma cell migration in vitro as well as metastatic tumor size in vivo. We also found that IL-6 is a target gene of miR-98, and IL-6 represses miR-98 levels via the Stat3-NF-κB-lin28B pathway. In an in vivo melanoma model, we demonstrate that miR-98 reduces melanoma metastasis and increases survival in part by reducing IL-6 levels; it also decreases Stat3 and p65 phosphorylation as well as lin28B mRNA levels. These results suggest that miR-98 inhibits melanoma metastasis in part through a novel miR-98-IL-6-negative feedback loop.

ACOX1 destabilizes p73 to suppress intrinsic apoptosis pathway and regulates sensitivity to doxorubicin in lymphoma cells

( Fei-meng Zheng ),( Wang-bing Chen ),( Tao Qin ),( Li-na Lv ),( Bi Feng ),( Yan-ling Lu ),( Zuo-quan Li ),( Xiao-chao Wang ),( Li-ju Tao ),( Hong-wen Li ),( Shu-you Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2019 BMB Reports Vol.52 No.9

Lymphoma is one of the most curable types of cancer. However, drug resistance is the main challenge faced in lymphoma treatment. Peroxisomal acyl-CoA oxidase 1 (ACOX1) is the rate-limiting enzyme in fatty acid β-oxidation. Deregulation of ACOX1 has been linked to peroxisomal disorders and carcinogenesis in the liver. Currently, there is no information about the function of ACOX1 in lymphoma. In this study, we found that upregulation of ACOX1 promoted proliferation in lymphoma cells, while downregulation of ACOX1 inhibited proliferation and induced apoptosis. Additionally, overexpression of ACOX1 increased resistance to doxorubicin, while suppression of ACOX1 expression markedly potentiated doxorubicin-induced apoptosis. Interestingly, downregulation of ACOX1 promoted mitochondrial location of Bad, reduced mitochondrial membrane potential and provoked apoptosis by activating caspase-9 and caspase-3 related apoptotic pathway. Overexpression of ACOX1 alleviated doxorubicin-induced activation of caspase-9 and caspase-3 and decrease of mitochondrial membrane potential. Importantly, downregulation of ACOX1 increased p73, but not p53, expression. p73 expression was critical for apoptosis induction induced by ACOX1 downregulation. Also, overexpression of ACOX1 significantly reduced stability of p73 protein thereby reducing p73 expression. Thus, our study indicated that suppression of ACOX1 could be a novel and effective approach for treatment of lymphoma. [BMB Reports 2019; 52(9): 566-571]