경막 내 colistimethate 투여로 치료한 다제 내성 cinetobacter baumannii 뇌실염

홍유아,유진홍,김진진,모은영,안건희,정희경,김진석,이현정,정미향,윤승배 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Acinetobacter species is a non-fermentative aerobic gram-negative coccobacillus that is an important pathogen found in nosocomial infections. Recently, multi-drug resistant Acinetobacter baumannii (MDR-AB) infections have been increasing and pose a serious problem. Most such infections present as bacteremia, pneumonia, or a wound infection; however, CNS infections are very rare. We herein present a case of ventriculitis caused by MDR-AB in a 37-year old man after a neurosurgical intervention. The patient was successfully treated with intrathecal colistimethate.

학습공동체 프로그램 질과 인지적 성취 간에 집단응집성, 협력적 자기조절의 매개효과

유지현(Yu, Ji-Hyun),이숙정(Lee, Sook-Jeong),권진백(Kwon, Jin-Baek) 한국경영교육학회 2021 경영교육연구 Vol.36 No.1

[연구목적] 본 연구는 비교과 프로그램 형태의 학습공동체 운영 개선을 위해 학습공동체 프로그램 질 척도를 개발하고, 학습공동체 프로그램의 질과 인지적 성취의 관계에 집단응집성 및 협력적 자기조절의 매개효과를 검증하고자 하였다. [연구방법] 학습공동체 관련 선행연구들과 S대학에서 운영한 학습공동체 만족도 조사 및 성찰일지를 분석하여 학습공동체 프로그램 질 척도 예비문항을 개발하고, 학습공동체에 참여한 대학생 299명을 대상으로 설문조사를 실시, 분석하였다. 또한 집단응집성 및 협력적 자기조절의 매개효과를 확인하기 위해 대학생 322명을 대상으로 설문조사를 실시, 분석하였다. [연구결과] 학습공동체 프로그램 질 척도는 11개 문항, 단일요인으로 개발하였으며, 탐색적 및 확인적 요인분석, 신뢰도 계수 산출을 통해 안정적인 척도임을 확인하였다. 또한 학습공동체 프로그램 질과 인지적 성취 간에 집단응집성 및 협력적 자기조절의 매개효과가 통계적으로 유의한 것으로 나타났다. [연구의 시사점] 본 연구에서 개발한 학습공동체 프로그램 질 척도를 활용함으로써 프로그램의 내실화 및 질 관리 방안을 수립할 수 있다. 또한 집단응집성 및 협력적 자기조절의 중요성을 규명함으로써 학습공동체 프로그램 운영의 시사점을 제공하였다. [Purpose] The purpose of this study was to develop the learning community program quality scale for improving a learning community program and to verify the mediating effect of group cohesion and collaborative self-regulation on the relationship between program quality and cognitive achievement. [Methodology] A questionnaire survey of 299 university students was conducted to develop the learning community program quality scale. To confirm the mediating effect of group cohesion and collaborative self-regulation, a questionnaire survey was conducted on 322 college students. [Findings] The learning community program quality scale was developed with 11 items and a single factor, and it was confirmed to be a stable scale. And, it was found that the mediating effect of group cohesion and collaborative self-regulation between the learning community program quality and cognitive achievement was significant. [Implications] Implications for the management of learning community program to promote group cohesion and collaborative self-regulation were provided.

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

Pseudomonas sp. CB-33이 생산하는 β-Xylosidase의 특성

유진환,김현구,김치경,임재윤 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

Pseudomonas sp. CB-33 균주의 배양 상등액으로부터 ammonium sulfate 침전법, PEI 침전법, DEAE-Sephadex column chromatography, gel column chromatography 그리고 preparative disc gel electrophoresis를 이용하여 β-xylosidase를 정제하였다. 정제된 효소의 Km 값은 4.6 mM이었다. 효소활성의 최적 pH는 7.0이었으며 pH 6.5~9 범위에서 안정하였다. 최적 활성온도는 45℃였으며, 각 온도에서 30분 동안 정치하였을 때 35℃까지 안정하였다. SDS-PAGE에 의해 효소의 분자량을 측정해 본 결과 약 44,000dalton이었다. HG^2+, Cu^2+ 그리고 Zn^2+는 효소활성을 저해하였고, 여러 화학변형제로 효소에 처리해 본 결과 tyrosine과 tryptophan이 효소활성에 관여하고 있을 것으로 추정된다. 여러가지 기질에 대한 특이성을 조사해 본 결과 pNPX 뿐만 아니라 pNPA도 분해하였다. Xylan에 endoxylanase만을 작용시켰을 때보다 β-xylosidase와 endoxylanase를 혼합작용 시켰을 때 xylan의 가수분해도는 약 2배 정도 증가하였다. 그리고 Pseudomonas sp. CB-33 균주가 생산하는 xylan 분해효소는 oat spelt xylan보다 birchwood xylan에 보다 효과적으로 작용하였다. The β-xylosidase was purified 99-fold from the culture supernatant of Pseudomonas sp. CB-33 by ammonium sulfate precipitation, PEI precipitation, DEAE-Sephadex column chromatography, Sephadex G-75 gel filtration chromatography and preparative disc gel electrophoresis. Molecular weight of the enzyme was estimated to be 44,000 by SDS polyacrylamide gel electrophoresis. The enzyme has a pH optimum for activity at 7.0 and is stable over pH 6.5~9.0. The optimal temperature of the enzyme was 45℃, and it enzymatic activity was completely inactivated at 55℃ for 30 min. Km value of the enzyme for p-nitrophenyl- β-D-xylopyranoside was calculated to be 4.6 mM. The effect of various reagents on the β-xylosidase activity was investigated. The enzyme activity was completely inhibited by Hg^2+, Cu^2+, and Zn^2+. The β-xylosidase was inactivated by tryptophan-specific reagent, N-bromosuccinimide and the enzyme was competitively inhibited by xylose. The β-xylosidase and endoxylanase from Pseudomonas sp. CB-33 hydrolized xylan synergically. The purified enzyme also showed α-L-arabinofuranosidase activity.

갓김치에서 분리한 캡사이신-내성 세균,Leuconostoc mesenteroides GK-10의 생리활성

김유진,나예린,이현호,오계헌 순천향대학교 기초과학연구소 2021 순천향자연과학연구 논문집 Vol.27 No.1.2

The aim of this work was to investigate the physiological activities of capsaicin-resistant bacterium, Leuconostoc mesenteroides GK-10 isolated from fully fermented mustard leaf kimchi. Initially, the morphological, physiological and biochemical characteristics of strain GK-10 were examined. Based on phylogenetic analysis using 16S rRNA sequencing, the isolate was identified as L. mesenteroides GK-10 and phylogenetic tree of the strain was plotted. L. mesenteroides GK-10 grew well in the different concentrations of capsaicin (0-600 μg/mL). Various physiological activities (e.g., tyrosinase inhibitory activity, ACE inhibitory activity, antioxidant activity, SOD-like activity, depletion of sodium nitrite) of L. mesenteroides GK-10 cultures with different concentrations of capsaicin were evaluated and compared to the cultures of L. mesenteroides DK-3 as a standard strain. According to the results, all of the physiological activities evaluated in this work were considerably higher in L. mesenteroides GK-10 cultures with 600 μg/mL capsaicin compared to L. mesenteroides DK-3 cultures with the same capsaicin concentration, although the physiological activities of GK-10 were higher or lower compared to those of DK-3 in the absence of capsaicin. In consequence, this work showed that L. mesenteroides GK-10 strains can maintain their physiological activities despite the presence of high concentrations of capsaicin.

쌀의 수침시간 및 증자시간에 따른 가래떡이 품질 특성

유진현,한규홍 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.6

The effects of soaking and steaming time on the quality characteristics of Korean rice cake (karedduk) were investigated. As the soaking time increased, the water-binding capacity and rice floor solid content also increased. After 8 to 10 hr soaking, 75.2 to 76.8% of particles were smaller than 40 gm, and 23.2 to 24.8% within the range of 40 to 100 ㎛. Therefore, an extended soaking time reduced the particle size. Texture is an important characteristic used to differentiate karedduk varieties, and is considered by the consumer as a determinant of the overall quality and preference. The hardness, cohesiveness, gumminess and chewiness of the karedduk structure decreased with increasing steaming time, but the springiness was highest after a 40 min steaming time. The overall texture properties were also more acceptable with 8 and 10 hrs soaking times. The L value of the karedduk decreased with increasing steaming time, but the b value increased. Also, the results of the sensory evaluation showed that an 8 hr soaking time and 40 min steaming time produced more acceptable karedduk in terms of whiteness, texture, springiness, chewiness and overall acceptability.

열전퇴형 적외선 검출기의 개발연구

유호종,이현철,김진섭,이종현 경북대학교 센서기술연구소 1997 연차보고서 Vol.1997 No.-

열전퇴형 적외선 검출기의 개발연구를 위해, 센서의 핵심 구성요소인 열차단막, 열전퇴 및 적외선 흡수체에서 각각 요구되는 특성들을 검토하였고, 이들 특성을 실현하는 공정기술의 검증을 위한 시험소자로서 적외선 열원 대신에 Pt 히터 또는 NiCr 히터가 내장된 Bi-Sb 열전쌍 또는 열전퇴를 제작하였으며, 이들의 열응답 특성을 주로 고찰하였다. Pt 히터 저항에 대한 다이아프램 형성전후의 전류-전압 특성 차이 및 이 히터가 내장된 Bi-Sb 열전쌍에 대한 공기 및 진공중에서의 열감도 변화로부터 Si_3N_4/SiO_2/Si_3N_4다이아프램의 열차단 특성을 확인할 수 있었고, 또한 히터의 저항 온도계수를 측정함으로써 인가된 전력에 의한 Pt 히터의 온도를 알 수 있었다. Pt 히터가 내장된 Bi-Sb 열전쌍 시험소자의 열기전력은 히터와 열전쌍 사이의 거리가 증가함에 따라 지수함수적으로 감소하였고, 열전쌍의 열전계수는 약 97㎶/K였다. Pt 또는 NiCr 히터가 내장된 Bi-Sb 열전퇴 시험소자의 전압감도는 열전퇴를 구성하는 열전쌍의 개수가 증가함에 따라 증가하였고, 히터와 열전퇴의 고온 접합부 사이의 거리 및 고온 접합부와 실리콘 림 사이의 거리에 크게 의존하였으며, 또한 진공중에서의 전압감도가 공기중에서의 전압감도보다 더 크게 나타났다. 상기 결과를 바탕으로 하여 열전퇴형 적외선 센서의 시험제작을 위한 공정 설계와 공정용 마스크의 설계 및 제작을 완료하였다. For the development of a thermopile-type infrared detector, the respective characteristics required in thermal isolation layer, thermopile and infrared absorber, which are the core components of the detector, were studied first of all. The Bi-Sb thermocouples and thermopiles with Pt- or NiCr-heater instead of the infrared radiation source have been fabricated as the test devices for the verification of the process technologies to fulfill respective characteristics, and their thermal responsivities were mainly investigated. The thermal isolation of Si_3N_4/SiO_2/Si_3N_4-diaphragm was confirmed by Ⅰ-Ⅴ characteristics difference of the Pt-heaters before and after the diaphragm formation, and the thermal sensitivities change of the Bi-Sb thermocouples in the air and vacuum. It could be known that the thermal loss in the heater is caused by the air, and the heater temperature with input power could be estimated by the measurement of the resistance temperature coefficient of Pt-heater. The thermoelectric voltage of the Bi-Sb thermocouple with Pt-heater decreased exponentially as the distance between heater and thermopile increased, and its thermoelectric coefficient was about 97 ㎶/K. The voltage responsivity of the Bi-Sb thermopile with Pt- or NiCr-heater increased as the number of thermocouples increase, was strongly dependent on the distance between heater and hot junction of the thermopile, and between hot junction and silicon-rim, and the voltage responsivity in the vacuum was higher than that in the air. On the basis of the above results, the process design and preparation of the process mask set for the test fabrication of a thermopile-type infrared sensor have been completed.

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

외상으로 실활된 미성숙 영구치에서의 계속된 치근 형성

강유진,김혜영,김영진,김현정,남순현 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4

치아에 외상을 받은 경우 치수 생활력의 상실은 흔한 일이다. 치수 생활력을 검사하는 방법으로는 임상적, 방사선학적으로 여러 가지가 있지만, 미성숙 외상치의 경우 일시적 현상과 가성 반응이 나타날 수 있으므로 치수 괴사에 대한 정확한 진단은 매우 어렵다. 생활력을 상실한 치아는 염증성 치근흡수, 치근단 낭종 등의 발생을 방지하기 위하여 치수 치료를 시행한다. 그러나 미성숙 영구치의 경우, 치수 치료를 시행 후 치근 성장이 정지될 수 있어 결과적으로 얇고 짧은 치근이 형성되어 장기적인 예후는 좋지 않다. 본 임상 증례에서는 외상으로 인하여 실활된 초기 영구치에서 치근단부의 최소한의 침습적 치근단 형성술로 계속된 치근 형성을 보여 이에 보고하는 바이다. In case of luxation injuries, loss of tooth vitality is common. And in case of trauma in the immature permanent teeth, precise diagnosis of pulp necrosis is very difficult. That is because limitation in distinguishing between normal dental papilla in immature permanent teeth, transient apical breakdown(TAB), which is part of normal healing process, and apical radiolucency in pulp necrosis. Especially in non-vital immature permanent tooth, the treatment is complex and requires long time. This clinical case report shows that severely infected immature teeth with periradicular periodontitis can undergo healing and apexogenesis or maturogenesis with no definative treatment or after conservative treatment. In the cases reported, we emphasize the considerable power of regeneration of the tooth, probably due to its large number of undifferentiated mesenchymal cells in the dental papilla, pulp tissue, periodontal ligament tissues. Thus, when endodontic treatment in immature permanent teeth, over instrumentation is not recommend for preserve the apical vital stem cells.

다공성 펠렛을 포집재로 사용하는 매연여과장치의 배압 및 재생 특성에 관한 연구

金鎭玄,柳鍾植,文學勳,車京玉 明知大學校 産業技術硏究所 2006 産業技術硏究所論文集 Vol.25 No.-

Diesel particulate trap is a core technology for the reduction of PM from diesel vehicles. This study presents the features and the characteristics of DPF system when using pellet type filters. In comparison with wall-flow filter, the pellet filter has the advantages of cracking free during regeneration and shape flexibility. Experiments are conducted in a test bench simulated as diesel engine exhaust condition. Pressure drop and particle loading rate was compared by using two pellet filters having the porosity of 70% and 0%. Also its regeneration was tested.