대구 유니버시아드대회 자원봉사활동에 대한 만족도 연구

황성동,성희자,고강호,전보경,권현수 慶北大學校 社會科學大學 2003 社會科學 Vol.15 No.-

Recombinant canstatin inhibits angiopoietin‐1‐induced angiogenesis and lymphangiogenesis

Hwang‐,Bo, Jeon,Yoo, Ki Hyun,Park, Jong‐,Hwa,Jeong, Han‐,Sin,Chung, In Sik Wiley Subscription Services, Inc., A Wiley Company 2012 International journal of cancer: Journal internati Vol.131 No.2

<P><B>Abstract</B></P><P>We describe the effect of recombinant canstatin, the NC1 domain of the α2 chain of Type IV collagen, on suppression of angiogenesis and lymphangiogenesis both <I>in vitro</I> and <I>in vivo</I>. Recombinant canstatin produced from stably transformed <I>Drosophila</I> S2 cells reduced the expression of angiopoietin‐1 in hypoxia mimetic agent, CoCl<SUB>2</SUB>‐treated CT‐26 cells. Recombinant canstatin inhibited proliferation, tube formation and migration of human angiopoietin‐1 (rhAngpt‐1)‐treated human umbilical vein endothelial cells (HUVEC) and lymphatic endothelial cells (LEC). Recombinant canstatin suppressed the expression of Tie‐2 and vascular endothelial growth factor‐3 (VEGFR‐3) transcripts in rhAngpt‐1‐treated HUVEC and LEC, respectively. The inhibitory effect of recombinant canstatin on tumor growth was also investigated using a heterotopic CT‐26 colon carcinoma animal (BALB/c mice) model. Recombinant canstatin reduced the final volume and weight of tumors, and blood and lymphatic vessel densities of tumors, which were evaluated by CD‐31 and LYVE‐1 immunostaining. Immunohistochemical analysis showed that recombinant canstatin dramatically reduced the expression of angiopoietin‐1 in CT‐26 colon carcinoma‐induced tumor, but not the expression of VEGF‐C. Tie‐2 and VEGFR‐3 expressions were also reduced in recombinant canstatin‐treated tumors. These results indicate that recombinant canstatin has anti‐tumoral activities against CT‐26 colon carcinoma cells. Recombinant canstatin reduces the expression of angiopoietin‐1 in hypoxia‐induced CT‐26 cells and inhibits the angiogenic and lymphangiogenic signaling induced by angiopoietin‐1. Recombinant canstatin probably inhibits angiogenesis and lymphangiogenesis <I>via</I> suppression of the integrin‐dependent FAK signaling induced by angiopoietin‐1/Tie‐2 and/or VEGFR‐3.</P>

유아의 또래관계 증진을 위한 학급 단위 프로그램의 개발 및 효과 검증

도현심,김민정,박보경,황영은 이화여자대학교 생활환경대학 인간생활환경연구소 2007 인간생활환경연구소 논집 Vol.- No.5

The purpose of this study was to develop a program for improving peer relations of preschoolers and to investigate its effectiveness, which was held in the natural setting of a classroom. The contents are consisted of understanding emotion, controlling negative emotion, and improving self-esteem, communication skills, and prosocial behaviors. The participants for the study were 105 4-year-o1d preschoolers who were divided into two groups such as experimental and control groups. Among them, 57 children were assigned for the experimental group, and 48 children for the control group. The experimental group participated in the program for 40-50 minutes per day once a week for 12 weeks during both the spring and fall semesters, while the control group received no treatment. As compared to the control group, children in the experimental group showed a significant increase in peer acceptance after they participated in the program, especially for both semesters.

연취급 근로자들의 사회 심리적 스트레스와 연노출 지표들간의 관련성

김용배,안현철,황보영,리갑수,황규윤,이성수,안규동,이병국 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목 적 : 연취급 근로자를 대상으로 사회 심리적 스트레스 수준을 알아보고 이를 기초자료로 활용하며 사회 심리적 스트레스와 연노출 지표들간의 관련성을 알아보고자 시도하였다. 방 법 ' 1차 연제련업종의 근로자 234명을 대상으로 일반적 특성, 사회 심리적 스트레스, 직업적 특성과 사회적 지지를 문진과 설문지를 통해 확인했으며 연노출 수준을 알기 위해 혈중 ZPP 농도, 혈중연농도, 요중 δ-ALA 배설량, 혈색소, 혈구용적치등을 측정했다. 결 과 : 단일변량 분석결과 혈중 연농도 수준과 직업적 특성에 따른 사회 심리적 스트레스는 유의한 차이가 있었으며 혈중 연농도가 증가함에 따라 스트레스 수준도 증가되는 경향을 나타냈다. 사회 심리적 스트레스와 연노출 지표 및 다른 변수들간의 관련성을 파악하기 위하여 상관분석을 실시한 결과 혈중 연농도만이 사회 심리적 스트레스와 양의 상관관계를 나타냈으며 유의했다. 결 론 : 고농도 연노출의 가능성이 있는 직종인 경우 기존의 연관련 자각증상에 대한 설문조사와 함께 사회 심리적 스트레스 측정도 병행하여 실시하는 것이 연에 의한 건강장해를 조기에 발견하고 예방하는데 필요하다. Objectives : This study was carried out to assess psychosocial distress of lead workers and to examine the relationship between lead exposure indices and psychosocial distress. Methods The study design was cross-sectional and research subjects were 234 lead workers in primary smelting industry. General characteristics, job characteristics and social support were obtained by self-administered questionnaire and history taking. Psychosocial distress was measured using PWI(Psychosocial Well-being Index), a 45-item self-administered instrument. Blood lead(PbB), zinc protoporphyrin(ZPP), δ-aminolevulinic acid in urine (ALAU) were selected as indicators of lead exposure. Results : There was significant difference in PWI according to lead exposure level and job characteristics. The results of correlation analysis showed that PWI was significantly correlated with PbB (r=0.203, p=0.002). Conclusions : Our results supported the association between lead exposure and psy-chosocial distress. The inclusion of psychosoclal distress measurement as a special health examination for lead workers was highly recommended for the effective health manage-ment.

입자상 오염물질의 대기환경기준에 관한 연구

허정숙,남보현,황인조,김동술 경희대학교 환경연구소 1999 環境硏究 論文集 Vol.9 No.-

We provide various fundamental statistics for PM-10 based on air quality monitoring data('96-'98) operated by the Ministry of Environment. The purposes of this paper are to examine the distribution of PM-10 level computed on the basis of a reference method adapted by USEPA and to review national ambient air quality standards for particulate matter in Korea. When we calcualted the 3-year('96-'98) average of the annual mean PM-10 concentrations at 24 monitoring sites. 21 sites were not met 50㎍/m(annual PM-10 standard in USA). Especially. Duckchon-dong and Shinpyung-dong sites at Pusan were recorded the highest concentration. Also, when we considered the 3-year average of the 99^(th) percentile values, 14 sites including Ssangmun-dong at Seoul and Duckchon-dong at Pusan among 23 were classified non-attainment area exceeding 150㎍/m(24-hour PM-10 standard in USA). We suggest that the air quality standards for PM-10 and PM2.5 in Korea must be actually regulated based on scientific review and the public health.

크롬 함유 페인트 분무작업자에서 발생한 비중격 천공

정희경,김두희,최병순,임현술,신용철,황보근 東國大學校醫學硏究所 1997 東國醫學 Vol.4 No.-

크롬은 산업에서 널리 사용되고 있으나 접촉성 피부염, 점막 자극, 비중격 천공, 천식, 폐암 등 건강에 영향을 미칠 수 있는데 크롬 및 그 화합물의 원자가, 수용성, 농도, 산성도, 폭로기간 및 경로에 따라 독성이 다르다. 약 5개월간 크롬에 폭로된 2명의 근로자에서 비중격 천공이 발생하여 35명 근로자의 작업조건과 건강상태를 조사하였다(페인트 분무 판넬부서 14명, 서스부서 16명, 기타 부서 5명). 공기 중 총크롬의 농도는 0.103-0.318 mg/m³이었고, 6가 크롬농도는 자동분무 중에 0.001-0.060 mg/m³, 수동분무 중에 0.029-0.226 mg/m³이었다. 모든 근로자에서 소변의 크롬농도는 50㎍/L를 넘지 않았으나, 혈액의 크롬농도는 3명에서 3㎍/dL를 초과하였다. 서스부서보다 판넬부서 근로자에서 비강의 미란이나 가피가 더 많이 발견되었다(교차비 7.0). 부서별로는 소변이나 혈액의 크롬농도가 차이가 없었고 비강소견에 따라서도 소변의 크롬농도가 차이가 없었으나, 혈액의 크롬농도는 비강의 미란이나 가피가 있는 경우 유의하게 높았다. 비중격 천공이 발생한 2명의 근로자에서 크롬에 의해 발생할 수 있는 폐나 비갑개, 간 및 신장의 이상소견과 천식은 발견되지 않앗다. 본 사업장에서 크롬에 의한 건강장애를 예방하기 위해서는 수동분무를 자동분무로 바꾸고 분무한 페인트가 완전히 건조한 후 다음 작업을 하여야 한다. 또한 크롬 미스트가 흡입되지 않도록 적절한 호흡기 보호구를 철저히 착용하여야 한다. After discovery, chromium has been widely used in industry. Health hazards of chromium and its compounds are contact dermatitis(allergic and irritant), mucosal irritation, nasal septal perforation, asthma, and lung cancer. Their toxicity depends on valency, solubility, concentration, PH, exposure duration and route. Nasal septal perforation was developed in two workers exposed to chromium for about 5 months, so we surveyed the working condition and health status of 35 workers(14 workers in paint spraying panel line, 16 in sus line and 5 in other line). Air concentration was as follows: total chromium 0.103-0.318 mg/m³, Cr(VI) 0.001-0.060 mg/m³in automatic spraying and 0.029-0.226 mg/m³in manual spraying. Urine chromium concentration was not over 50 ㎍/L in all workers, but blood chromium concentration was over 3 ㎍/dL in 5 workers. Erosion or crust of nasal cavity was detected more frequently in panel line workers than sus line workers(odds ratio=7.0). But blood or urine concentration of chromium was not different according to the job. Urine concentration of chromium was not different irrespective of nasal cavity finding, but blood concentration was high in the workers with erosion or crust of nasal cavity. Two workers with nasal septal perforation did not have any other diseases which can be developed by chromium exposure. To prevent health hazards of chromium in this factory, manual spraying process has to be changed to automatic process, the next process done after paint was absolutely dried, and respiratory protector should be weared to prevent inhalation of chromium mist effectively.

2.9% 과산화수소를 함유한 부착형 미백제의 임상적 효능과 안정성에 관한 연구

박은숙,성소래,홍성태,김지은,이소영,황수연,이신재,진보형,손호현,조병훈 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.4

2.9%의 과산화수소를 함유한 시험용 부착형 미백제(Medison dental whitening strip, Samsung medical Co.,Anyang,Korea)를 실험군 피험자 23명, 시험용 미백제에서 과산화수소를 제외한 동일한 제재를 대조약으로 대조군 피험자 24명에게 매일 1시간 30분씩, 2주간 적용하여 임상적 효능과 안전성을 평가하였다. 미백 효과는 Shade Vision, Chroma Mete 및 Vitapan classical shade guide를 이용하여 색변화를 측정하여 다음과 같은 결론을 얻었다. 1. 실험군에서의 전체 치아의 색변화량(ΔE^(*))은 세 가지 측정법에서 모두 대조군에 비해 통계적으로 유의한 차이를 보였고 (p < 0.05). 쉽게 인지할 수 있는 정도였다. 2. 색변화는 색좌표 상에서 명도의 증가와 녹색조 및 청색조로의 변화에 의해 얻어졌다. 3. 치아별로는 어두운 견치의 색변화량이 가장 컸고, 측절치, 중절치 순으로 감소하였다. 4. 47명의 피험자 중 5명이 경도의 과민증을 경험하였으나 곧 완화되었다, 치은염증지수와 전기치수검사 결과 시험 전후에 차이가 없어 안전성을 확인하였다. This study evaluated the effectiveness and safety of an experimental bleaching strip (Medison dental whitening strip, Samsung medical Co., Anyang, Korea) containing 2.9% hydrogen peroxide. Twenty-three volunteers used the bleaching strips for one and a half hour daily for 2 weeks. As control group, the same strips in which hydrogen peroxide was not included were used by 24 volunteers with the same protocol. The shade chang (ΔE^(*), color differnce) of twelve anterior teeth was measured using Shade Vision (X-Rite Inc., S. W. Grandville, MI, USA), Chroma Meter (Minota Co., Ltd. Osaka, Japan) and Vitapan classical shade guide (Vita Zahnfabrik, Germany). The shade change of overall teeth in the experimental group was significantly greater than that in the control group (p < 0.05) and was easily perceivable. The change resulted from the increase of lightness (CIE L^(*) value) and the decrease of redness (CIE a^(*) value) and yellowness (CIE b^(*) value). The shade change of individual tooth was greatest in canine, can smallest in central incisor. The safety of the bleaching strip was also confirmed. 〔J Kor Acad Cons Dent 31(4):269-281, 2006〕

Basic, HCC basic : O-033 ; Identification of genome variations associated with responsiveness of sorafenib by whole genome sequencing in patients with unresectable hepatocellular carcinoma

( Bo Hyun Kim ),( Joong Won Park ),( Yeon Su Lee ),( Byung Chul Kim ),( Sung Hoon Lee ),( Seung Hyun Hong ),( Jung Ah Hwang ),( Jin Sook Kim ),( Jung Ahn Lee ),( Jong Bhak ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Sorafenib is the first systemic agent to show a survival benefit in patients with advanced HCC. Because there is a substantial portion of poor-responders to sorafenib, reliable biomarkers to predict the treatment response before initiation are urgently needed. The aim of this study was to identify genome variations associated with responsiveness of sorafenib in unresectable HCC patients who were treated with concurrent TACE-sorafenib trial or sorafenib only by whole genome analysis using recently developed Next Generation Sequencing technology. Methods: We obtained normal genomic DNAs from four good responders (median TTP 10.8months) and three poor responders (median TTP 3.8months) and analyzed their genome by whole-genome sequencing using 90 bp paired-end reads. Identified variations were validated by Sanger sequencing and further functional analysis was performed. Results: On average, 90 gigabases per sample were produced at ∼34X sequencing depth, and they were mapped to the reference genome at an over 95% mapping rate. A total of 1813 variations were found and 138 are from sorafenib target- and drug ADME genes. Among them, 36 SNVs were found in the coding genes and 8 SNVs from 5 genes (ABCB1, ALDH3B1, FMO3, MUSK, SLC15A2) were non-synonymous SNVs which may cause a functional protein damage. Interestingly, pathway analysis of 1813 ns SNVs revealed that drug metabolism pathway components were highly affected (p <0.01), suggesting that the genetic differences in patients resulted in the different responses to sorafenib treatment. Conclusions: We present the results of whole genome sequencing approach to identify sorafenib response markers and related genes in unresectable HCC patients. We found genome variations in known sorafenib targets and ADME genes. These variations may offer a great resource to identify HCC patients whose genetic types are predicted as advantageous for sorafenib treatment.

Free Paper Session : Others ; Clinical features of Korean patients with autoimmune hepatitis-primary biliary cirrhosis overlap syndrome (초)

( Bo Hyun Kim ),( Sang Youn Hwang ),( Sook Hyang Jeong ),( Won Young Tak ),( Sang Hoon Ahn ),( Youn Jae Lee ),( Eun Uk Jung ),( Jung Il Lee ),( Jong Eun Yeon ),( Jae Seok Hwang ),( Soon Ho Um ),( Yeon 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3(S)

Extra-adrenal pheochromocytoma after operation of congenital heart disease

Bo-Hyun Hwang,Hyun-Young Kim,Sung-Eun Jung,Kwi-Won Park 대한외과학회 2012 Annals of Surgical Treatment and Research(ASRT) Vol.83 No.1

Extra-adrenal pheochromocytoma is rare and presents variable symptoms. Its difficulty to diagnosis delays appropriate treatment. We would like to report an unusual case of extra-adrenal pheochromocytoma. The patient came to the emergency room with dyspnea, palpitation, and cyanosis. He had a history of hospitalization for Fontan operation due to congenital heart disease. Despite medication, his blood pressure remained high. After additional laboratory and image exams, he was diagnosed with extra-adrenal pheochromocytoma and had surgical treatment. The final pathology report was extra-adrenal pheochromocytoma with high risk of malignancy. The postoperative course was uneventful and showed normal laboratory results even after 3 months of outpatient follow-up. Extra-adrenal pheochromocytoma presents variable symptoms. We should consider endocrinologic diseases like extra-adrenal pheochromocytoma in cases presenting with palpitation and high blood pressure, even with a past history of cardiac surgery.