갑상선 결절에 동반된 퇴행성 낭종의 재발과 낭액내 VEGF의 관련성

조은호,박희붕,김현만<SUP>1<.SUP>,이관우<SUP>1<.SUP>,정윤석<SUP>1<.SUP>,소의영,Eun Ho Cho,M.D.,Hee Boong Park,K.D.,Hyun Man Kim,M.D.<SUP>1<.SUP>,Kwan Woo Lee,M.D.<SUP>1<.SUP>,Yun Suk Jung,M.D.<SUP>1<.SUP> and Euy Young 대한갑상선-내분비외과학회 2002 The Koreran journal of Endocrine Surgery Vol.2 No.1

Purpose: VEGF, a potent angiogenic factor, increases vascular permeability and induces the fluid accumulation in the peritoneal and pleural cavities. This study is designed to know whether the VEGF concentration are associated with the formation of cystic fluid of thyroid nodules and the results of treatment. Methods: To know whether thyroid follicular cells produce VEGF, we measured the VEGF concentration in the conditioned medium after primary culture of the normal thyroid follicular cells (NT 1.0). Thirty seven patients, who had visited Ajou University Medical Center, were divided into two groups; group A (n=19) is patients whose cystic nodules disappeared completely with 1 or 2 times fine needle aspirations. In Group B (n=18), the cystic nodules recurred rapidly and required aspiration more than 3 times. Clinical records of patients were analyzed and compared with TSH and VEGF levels in cystic fluids. Results: The VEGF concentration in basal conditioned medium were 8.2⁑1.2 ng/ml in NT 1.0 and 8.2⁑0.6 ng/ml in thyroid cancer cell line (FTC-133). NT 1.0 was not affected by TSH stimulation. Mean concentration of TSH of all cystic fluids was 4.36μIU/ml (0.13∼21.7) and there was no difference between 2 groups. Mean concentration of VEGF of all cystic fluids was 140.7 ng/ml (11.1∼688.8). The VEGF concentration (287.9⁑289.6 ng/ml) in group B was significantly higher than that (104.2⁑97.1 ng/ml) in group A (P<0.05). Conclusion: This study suggests that thyroid follicular cells produce and secrete VEGF, and VEGF is related with the accumulation of cystic fluid in degenerative cysts of thyroid. Cysts with high VEGF concentration promote rapid reaccumulation of the cystic fluid and possibly necessitate operation in selected patients. (Korean J Endocrine Surg 2002;2:25- 30)

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

Clinical and Pathologic Features of Elderly Gastric Cancer

( Joo Hyun Lim ),( Dong Ho Lee ),( Cheol Min Shin ),( Na Yung Kim ),( Young Soo Park ),( Hyun Chae Jung ),( 1in Sung Song ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1

Background: Gastric cancer is one of the most common cancers in Korea and the incidence of which is great in elderly patients. However, not much is known about elderly gastric cancer. The aim of this study was to evaluate the specific features of gastric cancer in elderly patients. Methods: We reviewed medical records of 1107 patients who had undergone radical gastrectomy for gastric cancers between June 2005 and December 2009. All the patients were divided into two groups: Non-elderly group aged under 65 (n=676) and elderly group aged 65 and above (n=431). Results: There were more symptomatic patients among elderly group compared with non-elderly group (54.2% vs. 62.1%; p=0.009). Comorbidity was more prevalent in the elderly group (29.6% vs. 61.9%; p<0.001). The elderly group showed higher frequency of elevated tumor markers (CEA: 4.9% vs. 8.7%; p=0.041), CA19-9: 6.1% vs. 13.2%; p=0.001) and advanced diseases (42.5% vs. 49.4%, p=0.023).There were no significant differences in N stage (62.4% vs. 58.2%; p=0.164) or H. pylori status (63.6% vs. 57.5%; p=0.153) between the two groups. More cancers were located in lower third among the elderly group (45.1% vs 51.3%; p=0.045). The elderly group had more patients with Lauren intestinal type (40.7% vs. 58.2%; p<0.001) and well (8.0% vs. 13.7%; p=0.002) or moderately differentiated adenocarcinoma (29.6% vs. 44.1%; p<0.001). Rate of complication did not differ between the two groups (6.1% vs. 8.1%; p=0.187). Microsatellite instability (p<0.001) and p53 overexpression (p<0.001) were more frequent in the elderly group. Conclusion: Elderly gastric cancer showed a tendency of being diagnosed at more advanced stage with symptoms. However, it was related to pathologic features known for favorable prognosis except for p53 overexpression. Also surgery in elderly was as tolerable as in young patients. Thus we recommend to consider surgery more frequently for elderly patients with operable gastric cancers.

Cholelithiasis complicated with biliary sludge and urolithiasis in a dog

Seung-gon Lee,Dong-gun Kim,Joon-seok Lee,Ho-hyun Kwak,Hyun-sook Nam,Heung-myong Woo*, In-Chul Park**, Changbaig Hyun1,In-chul Park,Chang-baig Hyun 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.3

A 10-year-old intact female Miniature Schnauzer dog was referred with the primary complaint of persistentanorexia, remittent fever, vomiting and abdominal pain. Hemogram suggested a chronic inflammatory disease. Serumbiochemistry showed moderate hepatobiliary cellular damage with severe cholestasis. Abdominal radiography andultrasonography revealed hepatomegaly, choleliths and sludges in gall bladder and small stones in urinary bladder. Basedcholecystectomy and cystectomy, choleliths and uroliths were removed from gall bladder and urinary bladder,respectively. The clinical condition was dramatically improved after surgery.

FCT 5 : Fractional laser-assisted topical imiquimod 5% cream treatment for recalcitrant common warts in children

( Hyun Chang Ko ),( Jeong Min Kim ),( Hyun Ho Cho1 ),( Won Jeong Kim ),( Je Ho Mun ),( Margaret Song ),( Hoon Soo Kim ),( Byung Soo Kim ),( Moon Bum Kim ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Conventional destructive modalities for warts like cryotherapy or laser ablation have some limitations that are excruciating pain during procedure, in especially pediatric patients. Imiquimod is a topical immune response modifier that was approved for treating genital and perianal warts. But, thick stratum corneum of common warts may act as a barrier against drug permeation via the skin. Objectives: To evaluate efficacy and safety of above treatment. Methods: Eleven pediatric patients (6 female and 5 male, mean age 12.5 years) were included in this study. The lesions were treated by fractional 2940 nm Er:YAG laser for achieving the penetration of stratum corneum with 1 or 2 weeks intervals. Then, imiquimod 5% cream was self-applied once daily for 5 days a week. Assessment of response and adverse effects was performed every 2 weeks until complete clearance or up to maximum of 48 weeks. Pain during procedure was checked by VAS (0-10). Results: 8 out of 11 (72.7%) patients experienced complete clearance of all warts lesions. Mean duration of total treatment was 29.7 (16-48) weeks. Mean number of fractional laser treatment was 17.5 (8-37) times. No significant adverse effect was observed. VAS score of the pain during fractional laser treatment was 2.4 (1-4), comparing with 6.2 (5-8) during cryotherapy. Conclusion: Fractional ErYAG laser-assisted topical 5% imiquimod cream is an effective and safe treatment option for recalcitrant common warts of the children do not tolerate pain well.

항행 및 항법 : LX대한지적공사 위성측위 인프라 구축 및 네트워크 실시간 이동측위 성능 분석

하지현 ( Ji Hyun Ha1 ),김현호 ( Hyun Ho Kim ),김재복 ( Jae Bok Kim ) 한국항행학회 2014 韓國航行學會論文誌 Vol.18 No.6

대한지적공사 공간정보연구원에서는 네트워크 RTK의 효율성을 향상시키기 위하여 전국에 30개소의 LX 위성측위 인프라를 구축하고 MAC기반의 네트워크 RTK 서비스를 시험운영하고 있다. 본 논문에서는 LX 위성측위 인프라의 구축 현황을 소개하고 MAC기반의 네트워크 RTK 서비스의 측위 성능 분석 결과를 기술하였다. 성능 분석을 위하여 임시 고정점을 설치하고 국토지리정보원의 VRS와 동시관측을 실시하였다. 그 결과 두 인프라는 동서, 남북 방향으로 평균 1~2 ± 1 cm 차이를 보였으며 , 초기화 시간도 10초 이내에서 근소한 차이를 보였다. For improvement of network RTK performance in survey field, Spatial Information Research Institute (SIRI), LX Korea Cadastral Survey Corporation installed 30 GNSS permanent stations in Korea Peninsula, and has been running the MAC-based network RTK service as a test version. In this paper, we introduce the LX GNSS network and analyze the positioning accuracy of the LX MAC RTK service. For field test of the LX MAC RTK service, we installed temporally fixed anchor points and observed simultaneously with VRS of National Geographic Information Institute. As a result, the horizontal position differences and initial times of LX MAC with respect to NGII VRS are 1~2 ± 1 cm and <10 seconds, respectively.

Natural bone-mimicking nanoporeincorporated hydroxyapatite scaffolds for enhanced bone tissue regeneration

Chansong Kim,Jin Woong Lee,허준혁,Cheolhyun Park,Dai-Hwan Kim,Gyu Sung Yi1,Ho Chang Kang,Hyun Suk Jung,Hyunjung Shin,Jung Heon Lee 한국생체재료학회 2022 생체재료학회지 Vol.26 No.1

Background: A considerable number of studies has been carried out to develop alloplastic bone graft materials such as hydroxyapatite (HAP) that mimic the hierarchical structure of natural bones with multiple levels of pores: macro-, micro-, and nanopores. Although nanopores are known to play many essential roles in natural bones, only a few studies have focused on HAPs containing them; none of those studies investigated the functions of nanopores in biological systems. Method: We developed a simple yet powerful method to introduce nanopores into alloplastic HAP bone graft materials in large quantities by simply pressing HAP nanoparticles and sintering them at a low temperature. Results: The size of nanopores in HAP scaffolds can be controlled between 16.5 and 30.2 nm by changing the sintering temperature. When nanopores with a size of ~ 30.2 nm, similar to that of nanopores in natural bones, are introduced into HAP scaffolds, the mechanical strength and cell proliferation and differentiation rates are significantly increased. The developed HAP scaffolds containing nanopores (SNPs) are biocompatible, with negligible erythema and inflammatory reactions. In addition, they enhance the bone regeneration when are implanted into a rabbit model. Furthermore, the bone regeneration efficiency of the HAP-based SNP is better than that of a commercially available bone graft material. Conclusion: Nanopores of HAP scaffolds are very important for improving the bone regeneration efficiency and may be one of the key factors to consider in designing highly efficient next-generation alloplastic bone graft materials.

Effects of 27.12 MHz Radio Frequency on the Rapid and Uniform Tempering of Cylindrical Frozen Pork Loin (Longissimus thoracis et lumborum)

Eun Ji Choi,Hae Woong Park,Hui Seon Yang,Jin Se Kim1,Ho Hyun Chun 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.4

Quality characteristics of frozen cylindrical pork loin were evaluated following different tempering methods: 27.12 MHz curved-electrode radio frequency (RF) at 1000 and 1500 W, and forced-air convection (FC) or water immersion (WI) at 4ºC and 20°C. The developed RF tempering system with the newly designed curved-electrode achieved relatively uniform tempering compared to a parallel-plate RF system. FC tempering at 4°C was the most time-consuming process, whereas 1500 W RF was the shortest. Pork sample drip loss, water holding capacity, color, and microbiological quality declined after WI tempering at 20°C. Conversely, RF tempering yielded minimal sample changes in drip loss, microstructure, color, and total aerobic bacteria counts, along with relatively uniform internal sample temperature distributions compared to those of the other tempering treatments. These results indicate that curved-electrode RF tempering could be used to provide rapid defrosting with minimal quality deterioration of cylindrical frozen meat block products.

갑상선 미세유두상암의 임상적 특성

안재현,최재영<SUP>1<.SUP>,박원서,송정윤,김용호,이상목,고석환,Jae Hyun Ahn,M.D.,Jae Young Choi,M.D.<SUP>1<.SUP>,Won Seo Park,M.D.,Jeong Yoon Song,M.D.,Yong Ho Kim,M.D.,Sang Mok Lee,M.D. and Suck Hwan Koh,M.D. 대한갑상선-내분비외과학회 2008 The Koreran journal of Endocrine Surgery Vol.8 No.2

<B>Purpose:</B> PTMC is defined as a papillary thyroid cancer smaller than 10 mm in its greatest diameter. It is the most common form of differentiated thyroid cancer and its prognosis is known to be very favorable. The aim of this study is to identify its biologic behavior and to formulate a reasonable therapeutic strategy for the treatment of PTMC. <B>Methods:</B> 379 patients with papillary thyroid cancer were analyzed. Each patient was diagnosed preoperatively or postoperatively and treated between Jan. 2000 and Dec. 2007. Among these patients, 143 had been identified as having PTMC (37.7%) with a mean tumor size of 0.72 cm in diameter. <B>Results:</B> There were no significant differences of the clinical characteristics such as gender, age, the operative methods, or multicentricity between the PTMC group and the non-PTMC group, except for LN metastases. Also, there were fewer symptoms of palpable neck mass and preoperative findings such as capsular invasion and microcalcification in the PTMC group. We performed unilateral lobectomy with or without central compartment neck node dissection for the early stage diseases, but for the later stages of disease we performed near-total or total thyroidectomy with routine central compartment neck node dissection. And for the patients with lateral node enlargement, we performed ipsilateral modified radical neck dissection (MRND). <B>Conclusion:</B> This study shows that PTMC is quite similar to conventional papillary thyroid cancer in its biological behavior, and we conclude that total thyroidectomy with central compartment neck node dissection is the proper therapeutic strategy to treat PTMC. However, further study is necessary for identifying the low-risk and high-risk patients with PTMC. <B>(Ko</B><B></B><B>rean J Endocrine Surg 2008;8:101-105)</B>

A Case of Favorable Responses after Gefitinib in a Patient with EGFR Mutated Adenosquamous Lung Carcinoma

Nam Jun Cho,Ho-Sung Lee,Si-Hyong Jang,Jae Sung Choi,Ju Ock Na,Ki Hyun Seo,Yong Hoon Kim,Hyo-Chul Kang,Dae-Yeon Kim1 순천향의학연구소 2013 Journal of Soonchunhyang Medical Science Vol.19 No.2

In patients with advanced non-small cell lung cancer harboring epidermal growth factor receptor (EGFR) mutations, the epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are used as first treatment option. Because adenosquamous carcinoma (ASC) is a rare histologic subtype, evidences about EGFR-TKIs as first treatment option for advanced ASC are lacking. We report a case of an advanced ASC patient with the EGFR mutation, who showed good responses during 4-month treatment with gefitinib. And we will review about a necessity of EGFR mutation test and efficacy of EGFR-TKIs in ASC patients from the recent studies.