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김형진,이지영,권혁윤 한국공업화학회 2004 응용화학 Vol.8 No.1
This study was carried out to evaluate the application possibility of UF Membrane System to papermaking industry in order to optimize the recycling of white water. In this study, for the efficiency test of UF modules, the MWCO(molecular weight cut-off) of the membrane was estimated by the COD measurement from UF filtrate water. From the rejection rate of membrane, three kinds of UF module were selected and evaluated in the factors of COD, SS, turbidity and flow rate.
김경아,남혜윤,문제혁,정진숙,임영,정치경 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4
목적 : Rat2 섬유모세포에서 α-quartz에 의해 유도된 NOS의 조절기전을 알아보기 위하여 본 연구가시도 되었다. 방법 : Rat2 세포에 있어서 α-quartz 자극에 의한 NO와 H₂O₂의 분비 및 iNOS의 발현을 관찰하였고, iNOS 억제제인 L-NIL과 H₂O₂ 억제제인Catalase를 전처리하고 마찬가지로 NO와 H₂O₂분비 그리고 iNOS의 단백발현 정도를 western blot-ting을 이용하여 관찰하였다. 결과 : o-quartz는 Rat2 세포에서 iNOS에 의한NO와 H₂O₂의 분비를 유도하였다. L-NIL은 R3t2세포에서 D-quartz에 의한 H₂O₂의 분비와 iNOS발현을 완전히 억제하였다. Catalase로 전처리 하였을 때 o-quartz로 유도된 iNOS에 의하여 생산증가된 H2Oa가 iNOS를 억제하는 autoinhibitorypathway가 차단되어 결과적으로 H₂O₂의 분비와 iNOS의 발현이 증가하였다. 결론 : Rat2 세포에서 α-quartz에 의하여 유도된iNOS는 H₂O₂의 분비를 촉진하고 이 H₂O₂는 iNOS기능을 조절하는 자가조절기전에 중요한 역할을 하는 것으로 생각된다. Objectives : This study was performed in order to investigate the molecular mechanism regulating nitric oxide synthase (NOS) induced by α-quartz in Rat2 fibroblast. Methods : α-quartz-induced nitric Oxide (NO) anti H₂O₂ formation anti α-quarts-induced iNOS protein expression in Rat2 fibroblast were monitored. With iNOS inhibitor (L-N6- (1-iminoethyl) lysine hydrochloride, L-NIL) or antioxidant (catalase), we observed NO and H₂O₂ formation and iNOS protein expression in Rat2 fibroblast stimulated with α-quartz. Results : α-quartz stimulated iNOS-induced NO and H₂O₂ formation in Rat2 fibroblast. L-NIL inhibited H₂O₂ formation and iNOS protein expression by α-quartz in Rat2 fibroblast. Pretreatment with catalase blocked the autoinhibitory pathway of iNOS by iNOS-induced H₂O₂ therefore H₂O₂ and NO production and iNOS protein expression were increased in Rat2 fibrobalst stimulated with α-quartz Conclusion : α-quartz-induced iNOS Stimulated H₂O₂ formation in Rat2 fibroblast. INOS-induced H₂O₂ by α-quartz plays an important role in the autoinhibition pathway for regulating the iNOS function in Rat2 fibroblast
장성동,이은희,홍명엽,문성진,김주혁,이윤관,김영준 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1
This study is purposed to analyse the effects of long-term & one-time 10km running exercise from bone metabolic marker change on bone metabolism. 7 male members(18.6±1.08) from a marathon club were subjected to the study. They were allowed to do long-term 10 km running training once a week for 6 months. And bone metabolism mark was determined according to the respective times : before/after 6 months training, before/after 10 km race, one day after the race, 3 days after the race. The examination items of bone metabolism mark were serum calcium, phosphorus, paratyroid hormone, osteocalcin, calcitonin, alkaline phosphatase in blood and calcium, phosphorus, deoxypyridinolin in urine. The results of study were as follows; 1) There was no change in deoxypyridinolin and alkaline phosphatase but there was significant change in calcitonin, paratyroid hormone and osteocalcin(p<.01). 2) There was no change of Calcium in serum and urine but there was significant change of phosphorus in blood(p<.01). To make a conclusion, it is determined that long-term 10 km running at low intensity and low frequency tends to restrict bone metabolism circulation, but exercise at a little high level activates bone metabolism into building up bones and absorbing bones and finally shows up high bone metabolism circulation, which is inferred to have an effective influence on bone health. It is considered there is a possibility that different exercise intensity, even same kind of exercise, influences on bone metabolism sdifferently.
Expression of human norovirus VP1 gene and VP1-specific monoclonal antibodies
Jin-Won Kim,Yun-Ju Kong,Myeong-Seob Kim,Hyeok-Jin Lee,Shien-Young Kang,Sang-Won Lee 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.3
Norovirus (NoV) is an etiologic agent of human and animal acute gastroenteritis and is a member of the family Caliciviridae. NoV is classified based on nucleotide sequences of the VP1 gene into at least six genogroups (GI-GVI), among which GI, GII, and GIV are known to infect humans and GII is the most prevalent genogroup. In this study, VP1, the full gene of GII human NoV, was cloned from a human fecal sample and expressed using a baculovirus expression system. Human NoV VP1-specific monoclonal antibodies (MAbs) were produced using expressed recombinant VP1. Expressed VP1 in the recombinant virus was confirmed by polymerase chain reaction (PCR), indirect fluorescence antibody (IFA) test, and Western blot analysis. Eight hybridomas secreting VP1-specific MAbs against human GII NoV were generated and characterized. All of the MAbs produced in this study reacted with human GII NoV VP1-recombinant baculoviruses but not with other non-human calicivirus recombinant baculoviruses. These MAbs reacted specifically with human NoV GII.4-2009 virus-like particles (VLPs), and some MAbs showed cross-reactivity with other GII.4 variant VLPs. Expressed human GII NoV VP1-recombinant protein and MAbs specific to this protein can be used as useful reagents for detecting and characterizing human NoV.
Hyeok-Jin Yun,Ho-Sung Kim,Myung-Hyo Ryu,Ju-Won Baek,Hee-Je Kim 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6
In this paper, a simple voltage balance method for a SST using cascaded H-bridge converters is proposed to reduce the voltage difference of H-bridge converters. The tolerance of DAB converter’s passive components in each secondary stage causes the unbalance problem of output voltage in primary side H-bridge converter. In order to reduce the voltage difference of H-bridge converters, the additional voltage balance controller is merged in DAB converter’s voltage controller. By using the proposed voltage balance controller, the modified phase shift angle is applied for each DAB converters. Due to the proposed control methods, the dc-link voltage through each H-bridge converters can be balanced. Compared to the conventional methods, the proposed balance method does not require the additional current sensors for voltage balance control. The design and performance of the proposed SST are verified by experimental results using a 5kW prototype.
하이드로겔 처리가 토양의 질 및 상추 생육에 미치는 영향
윤혁진 ( Hyeok-jin Yun ),박병준 ( Byung-jun Park ),이찬규 ( Chan-gyu Lee ),김연호 ( Yeon-ho Kim ),김혁수 ( Hyuck-soo Kim ),남성현 ( Sung-hyeon Nam ),정재영 ( Jae-young Jung ) 한국환경농학회 2022 한국환경농학회 학술대회집 Vol.2022 No.-
지구 온난화 토양수분의 감소는 가속화 되고 있으며, 지구 평균 기온이 2도 상승 시 전세계 면적의 23%가 토양 수분 감소로 인한 심각한 피해를 입을 것이라는 예측 결과가 보고되었다. 따라서 이러한 위협요인으로부터 토양 수분을 지속적으로 유지하기 위한 방안 중 하나로 하이드로겔(Hydrogel) 적용을 고려할 수 있다. Hydrogel은 친수성 가교 망상 고분자 물질로 수분 공급 시 이를 흡수해 팽창하고, 수분 부족시 흡수한 물을 다시 방출하는 능력이 있어 건조 조건에서 식물 생육에 긍정적인 영향을 미칠 수 있다. 또한, 토양 수분 함량의 지속적 유지는 토양의 질에도 긍정적인 변화를 유도할 수 있다. 본 연구에서는 Hydrogel 처리에 따른 토양의 물리, 화학적, 생물학적특성 변화와 작물의 생육 변화를 관찰하기 위해 토양 배양 실험과 상추 재배 실험을 진행하였다. 본 실험에서는 2가지 토성(Sandy loam, Loam)을 지닌 토양을 준비하였으며, 토양에 0, 0.5, 1, 2% (w/w)의 Hydrogel을 각각 처리한 후, 토양의 30% 수준의 수분을 공급해 안정화한 뒤, 50일 간 토양의 물리, 화학, 생물학적 변화를 조사하였다. 그 결과 Hydrogel의 처리량이 증가함에 따라 토양의 수분 함량은 증가하고 용적밀도는 감소하는 것을 확인할 수 있었다. 또한 상추의 생장에도 차이를 나타냈는데, Hydrogel 처리량에 비례하여 식물의 건중량이 증가하는 것을 확인할 수 있었다. 토양 배양 시험에서 토양의 물리적 특성(용적밀도 등) 외에 화학적, 생물학적인 특성은 Hydrogel 처리에 따른 유의적인 차이는 확인할 수 없었다. 결과적으로 Hydrogel을 토양에 처리시 작물의 수분 공급을 원활히 하여 작물 생육에 긍정적 영향을 미치는 것을 확인하였다. 하지만 토양 대비 1% 이상의 Hydrogel을 처리하면 토양의 용적밀도가 식물 재배 적정기준 이상으로 낮아져 실제 토양에 적용을 위한 적정 처리량 설정이 요구되며, 장기 모니터링을 통한 토양 화학, 생물학적 특성 변화 조사가 필요할 것으로 판단된다.