고수의 향미성분에 관한 연구

황금희,강성구,최옥자,김용두,김경자 한국조리과학회 2001 한국식품조리과학회지 Vol.17 No.1

This study was carried out to investigate to proximate compositions, free sugars, organic acids, amino acids, and volatiles from the fresh leaf, root and seed of coriander. The research results are as follows : Moisture was 79.93% in the leaf, 81.89% in the root. Crude protein, crude lipid and crude ash were the highest in the seed. Ascorbic acid was 65.41㎎% in the leaf and 37.83㎎% in the root. Glucose, fructose and sucrose were the major free sugars. Glucose was 7.92㎎% and fructose 7.51㎎% in the leaf. Sucrose was 17.34㎎% in the root, highest level. Among organic acids, malic acid was 354.55㎎% in the leaf, the highest level. The content rate of organic acids was high in the order of leaf, seed and root. The content rate of total amino acid was high in the order of seed, root and leaf. Glutamic acid and aspartic acid were high in the leaf and root. Glutamic acid and proline were high in the seed. The content rate of free amino acid is the same as that of total amino acid. Glutamic acid and serine were high in the leaf and seed. Glutamic acid and treonine were high in the root. The contents of total amino acid in each parts of the coriander was higher than that of free amino acid, The composition of amino acid in the total amino acid and free amino acid was different. The volatile constituents were extracted by steam distillation method and analyzed by GC-Mass. The content of the volatile constituents was 45.31㎎% in the leaf, (E)-2-decenal was the highest, followed by decanal, 2-dodecenal, (E)-2-decen-1-ol in order, aldehyde and alcohol was major constituents. The content of the volatile constituents was 36.01㎎% in the root and 54.37㎎% in the seed. linalool was the highest in the root and seed. it was 22.27%, 53.67% in root and seed.

Effects of Coriandrum Sativum L. on Lipid Metabolism in Rats with Hypertriglyceridemic Diet

Geum-Hee Hwang,Young-Ran Heo,Heung-Jae Lee,Ok-Ja Choi,Seong-Koo Kang,Yong-Doo Kim 한국영양학회 2001 Nutritional Sciences Vol.4 No.1

A novel anti-melanogenic agent, KDZ-001, inhibits tyrosinase enzymatic activity

Hwang, Kyu-Seok,Yang, Jung Yoon,Lee, Jooyun,Lee, Yu-Ri,Kim, Seong Soon,Kim, Geum Ran,Chae, Jin Sil,Ahn, Jin Hee,Shin, Dae-Seop,Choi, Tae-Young,Bae, Myung Ae Elsevier 2018 Journal of dermatological science Vol.89 No.2

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>The demand for anti-melanogenic agents is increasing due to the unwanted side effects of current treatments. To find an effective anti-melanogenic agent, we used zebrafish as a whole animal model for phenotype-based drug and cosmetic discovery screening.</P> <P><B>Objectives</B></P> <P>The aim of this study was to identify and explore a small molecule that could be used for skin-whitening cosmetics.</P> <P><B>Methods</B></P> <P>Using zebrafish embryos, we examined the effects of 1000 compounds on zebrafish development and pigmentation. Pigmentation production was assessed by tyrosinase (TYR) enzymatic activity and melanin contents. Pigmentation marker expression in the human melanoma cell line HMV-II was analyzed by western blot. We also tested reconstituted human skin tissue and analyzed KDZ-001 with computational molecular modeling.</P> <P><B>Results</B></P> <P>We identified three compounds that affected the pigmentation of developing melanophores in zebrafish. Among them, we identified KDZ-001, a novel anti-melanogenic agent, which strongly inhibits melanin synthesis in the developing melanophores of zebrafish, HMV-II cells, and reconstituted human skin with no toxicity. We found that KDZ-001 directly inhibits TYR enzymatic activity. Notably, computational molecular modeling of KDZ-001 suggested that its interaction with copper ions in the active site of TYR is essential for melanin synthesis, further demonstrating that KDZ-001 mainly acts as a TYR inhibitor to synthesize melanin.</P> <P><B>Conclusion</B></P> <P>KDZ-001 inhibits melanin synthesis and has a potential for use in skin-whitening cosmetics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In this study, we show that KDZ-001, a novel anti-melanogenic agent, strongly inhibits melanin synthesis in the developing melanophores of zebrafish, HMV-II cells, and reconstituted human skin with no toxicity. We also show that KDZ-001 directly inhibits TYR enzymatic activity. Thus, KDZ-001 is a potential candidate for a skin-whitening agent in the cosmetic industry. </LI> </UL> </P>

Stimulation of angiogenesis and survival of endothelial cells by human monoclonal Tie2 receptor antibody

Hwang, Byungtae,Lee, Sang-Hyun,Kim, Jang-Seong,Moon, Ji Hyun,Jeung, In Cheul,Lee, Na Geum,Park, Jongjin,Hong, Hyo Jeong,Cho, Young-Lai,Jung, Haiyoung,Park, Young-Jun,Lee, Seon-Jin,Lee, Hee Gu,Kim, Won Elsevier 2015 Biomaterials Vol.51 No.-

<P><B>Abstract</B></P> <P>Angiopoietin-1 (Ang1) and its endothelium-specific receptor, tyrosine kinase with Ig and epidermal growth factor homology domain 2 (Tie2), play critical roles in vascular development. Although the Ang1/Tie2 system has been considered a promising target for therapeutic neovascularization, several imitations of large-scale production have hampered the development of recombinant Ang1 for therapeutics. In this study, we produced a fully human agonistic antibody against Tie2, designated 1–4h, and tested the applicability of 1–4h as an alternative to native Ang1 in therapeutic angiogenesis. 1–4h significantly enhanced the phosphorylation of Tie2 in a dose- and time-dependent manner in human Tie2-expressing HEK293 cells and human umbilical vein endothelial cells. Moreover, 1–4h induced the activation of Tie2-mediated intracellular signaling such as AKT, eNOS, MAPK, and Focal Adhesion Kinase p125<SUP>FAK</SUP>. In addition, 1–4h increased the chemotactic motility and capillary-like tube formation of endothelial cells <I>in vitro</I> and enhanced the survival of serum-deprived endothelial cells. Taken together, our data clearly suggest that a human Tie2 agonistic antibody is a potentially useful therapeutic approach for the treatment of several ischemic diseases including delayed-wound healing and ischemic heart and limb diseases.</P>

Effects of Coriandrum Sativum L. on Lipid Metabolism in Rats with Hypertriglyceridemic Diet

Hwang, Geum-Hee,Heo, Young-Ran,Lee, Heung-Jae,Park, Ok-Ja,Kang, Seong-Koo,Kim, Yong-Doo The Korean Nutrition Society 2001 Nutritional Sciences Vol.4 No.1

This study was designed to investigate the effects of Coriandrum Sativum L. on lipid metabolism in rats with hypertriglyceridemia. Also, we compared the effects of the leaf, seed and root of Coriandrum Sativum L. on lipid metabolism in rats with hypertriglyceridemia. Hypertriglyceridemia was induced in 32 male Sprague-Dawley rats weighting 108$\pm$13 g through the feeding of high-fat diets containing 20% fat and 5% cellulose for ten days. The rats were divided into four groups as follows : control (C), leaf (L), seed (S) and root (R) groups. For the five-week experimental period, the C group was fed the above diet an the L, S and R groups were fed Coriandrum Sativum L. diets containing 5% dry leaf, seed and root of Coriandrum Sativum L., respectively. Intake of diet and weight gain were significantly lower in the C group than in the L, S and R groups. But there was no significant difference among the L, S and R groups. Because of weight differences among the groups, all obtained data was adjusted to weight. The level of plasma total cholesterol was not significantly different among the four groups. But after adjusting to weight differences, the level of plasma triglyceride was significantly higher in the C groups than in the L and S groups. These results suggest that dietary Coriandrum Sativum L. may increase appetite and have an inhibitory effect on the lipid metabolism of rats with hypertriglyceridemia. Also, those effects may be partly different (leaf, root and seed) from those of Coriandrum Sativum L.

식이내 고수(Coriandrum sativum L.)의 첨가가 당뇨성 흰쥐의 혈장과 간의 지질함량에 미치는 영향

황금희(Geum-Hee Hwang),윤연희(Yeon-Hee Yoon),최인선(In-Seon Choi),최옥자(Ok-Ja Choi),강성구(Seong-Koo Kang),김용두(Young-Doo Kim) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.4

본 연구에서는 향신료로 사용되고 있는 고수의 첨가가 당뇨성 흰쥐의 혈당, 혈청 및 간조직의 지질 수준 저하효과와 각 부위별 효능을 규명하고자 평균체중이 108±13 g인 Sprague Dawley종 숫쥐 32마리를 사용하여 streptozotocin(St. Louis, USA)을 흰쥐 체중 kg당 65 mg씩 복강내 주사하여 당뇨를 유발시킨 후 4주간 실험하였다. 식이섭취량과 체중을 매일 측정하였으며 이로부터 식이효율을 구하였고, 혈당, 혈장, 간 및 근육 조직의 인슐린 농도, 지질, 과산화지질 및 비타민 E의 함량을 비교분석하였다. 즉 당뇨병성 고지혈증쥐는 일반 식이를 기본식이로 하였으며, 고수급여군은 분말화한 고수(잎, 씨, 뿌리)를 5% 일정하게 배합하였고, 대조군은 고수의 평균 섬유소 함량을 고려하여 cellulose 5%로 대체하였다. 본 실험에서 식이섭취량, 체중증가량 및 이들로부터 계산한 식이효율은 고수의 섭취에 의해 영향을 받지 않았으며, 신장, 비장, 고환 및 췌장의 무게는 고수군과 대조군 사이에 유의한 차이가 없었고, 간과 심장무게는 유의한 차이를 보였으며, 특히 간 무게는 그차이가 현격하였다. 고수의 급여로 혈장 인슐린의 농도는 증가하는 경향이었으며, 혈당치는 오히려 증가하였다. 혈장의 콜레스테롤 농도가 고수 뿌리의 섭취로 대조군보다 유의적으로 낮은 경향을 보였으나 HDL-cholesterol 농도에는 영향을 주지 못하였다. 혈장과 간의 지질과산화물 함량은 고수뿌리군이 고수잎, 대조군보다 낮은 경향으로 체내의 지질 과산화물을 억제 할 수 있는 가능성을 나타내었고, 비타민 E의 함량은 간조직에서 고수 잎과 씨 급여군에서만 증가하는 경향을 보였다. 혈장에서는 모든 고수급여군이 그리고 간조직에서는 뿌리급여군에서 대조군에 비해 비타민 E의 함량이 감소되는 경향을 보이는 바 쥐에서 지질과산화물이 증가된 만큼 비타민 E가 항산화제로 이용되어 그 함량이 저하된 것으로 보여진다. 이상의 결과에서와 같이 당뇨병에 있어서 고수는 혈당 강하효과를 나타내 주지는 못하였으나 고수의 뿌리가 혈장의 지질수준을 낮추었고, 비타민 E의 함량을 감소시켜 혈장과 간조직의 과산화지질 생성을 억제시키는 효과가 나타나 콜레스테롤 수준을 낮출 수 있고, 항산화성을 보이는 향신료 또는 식품재료로서의 개발 가능성이 있음이 시사되었다. This study was designed to investigate the effects of Coriandrum sativum L. on lipid metabolism in nondiabetic and streptozotocin-induced diabetic. Thirty two male Sprague-Dawley rats weighting 108±13 g were divided into four groups as follow : control (C), leaf (L), seed (S) and root (R) groups. For four weeks of experimental periods, the C group were fed basal diet and the L, S and R groups were fed Coriandrum sativum L. diets containing 5% dried leaf, seed and root of Coriandrum sativum L., respectively. Diet intakes, weight gain and food efficiency were not significantly different among the four groups. The liver and heart weights of L, S and R groups were significantly higher than the control group. The levels of plasma insulin was significantly increased but the plasma glucose levels was not decreased in all Coriandrum group. The levels of plasma lipids was not significantly different among the four groups. The levels of plasma total cholesterol was significantly decreased in R groups. The levels of plasma and liver TBARS values were significantly decreased in R groups. The levels of plasma vitamin E were tendency to decrese in all coriandum groups. These results indicate feeding diet with coriander did not show the hypolipidemic effect but show the antioxidative effect.

척담(滌痰).축어(逐瘀).고본치료효천적경험(固本治療哮喘的經驗) -척담(滌痰).축어(逐瘀).고본(固本)의 방법에 따른 천식에 관한 치험-

황금성,Hwang Geum-Seong 대한한방소아과학회 2001 대한한방소아과학회지 Vol.15 No.1

본 논문은 척담(滌痰) 축어(逐瘀) 고본(固本)의 방법으로 천식을 완화시킨 치험에 관한 내용이다. 천식의 병인병기는 폐(肺) 비(脾) 신(腎) 삼장(三臟)의 본허(本虛)로 인한 알레르기성 체질이 내적인 근본요인으로 간주된다. 담어복폐(談瘀伏肺)는 천식의 병리적인 결과로서 증상유발의 요인이기도 하다. 즉 담어(痰瘀)는 천식의 병리적인 부산물이며, 동시에 천식을 일으키는 치병요인이 된다. 담어(痰瘀)가 내복(內伏)하여 철저히 제거되지 않으면 천식은 결코 완치될 수 없다. 이와 같은 한의학적인 관점은 현대의학에서 천식을 기도의 만성 염증에 의하여 유발된 증상으로 파악하고 있는 기전과 일맥상통하다. 또한 담(痰) 어(瘀) 허(虛)는 천식의 병리변화 과정에서 전반적으로 영향을 미치고 있다는 인식이 지배적이며, 이는 천식치료를 위한 처방구성에 이론적 근거를 제시하고 있다. 치료: 천식은 발작기와 완화기로 분류하여 치료하며, 발작기는 냉효(冷哮)와 열효(熱哮)로 구분된다. 냉효(冷哮)의 경우 치료원칙은 온폐산한(溫肺散寒), 척담평천(滌痰平喘), 화어행체(化瘀行滯)를 기본으로 한다. 처방은 구마황(灸麻黃) 행인(杏仁) 황금(黃芩) 세신(細辛) 계지(桂枝) 오미자(五味子) 당귀(當歸) 울김(鬱金) 목단피(牧丹皮) 지룡(地龍) 정력자(?歷子) 담남성(膽南星) 황기(黃?) 감초(甘草) 등을 기본적으로 사용한다. 열효(熱哮)의 경우 치료원칙은 청열사폐(淸熱瀉肺) 척담평천(滌痰平喘), 화어행체(化瘀行滯)를 근본으로 삼고, 처방으로는 구마황(灸麻黃) 행인(杏仁) 황금(黃芩) 목단피(牧丹皮) 적작약(赤芍藥) 당귀(當歸) 정력자(?歷子) 담남성(膽南星) 지룡(地龍) 어성초(魚腥草) 포공영(蒲公英) 황기(黃?) 감초(甘草) 등을 기본적으로 사용한다. 완화단계도 역시 두가지 유형으로 나누어지는데, 폐신음허형(肺腎陰虛型)은 익기양음(益氣養陰) 척담행어(滌痰行瘀)를 치료원칙으로 하고, 처방에는 남북사삼(南北沙蔘) 구자울(灸紫?) 및 관동화(款冬花) 당귀(當歸) 목단피(牧丹皮) 울김(鬱金) 정력자(?歷子) 세신(細辛) 오미자(五味子) 구기자(枸杞子) 산수황육(山茱黃肉) 황기(黃?) 감초(甘草) 등을 기본적으로 사용한다. 비신양허형(脾腎陽虛型)은 온보비신(溫補脾腎) 화어척담(化瘀滌痰)을 원칙으로 하며, 처방에는 구마황( 灸麻黃) 세신(細辛) 오미자(五味子) 당귀(當歸) 단삼(丹蔘) 울김(鬱金) 정력자(?歷子) 반하(半夏) 보골지(補骨脂) 선령비(仙靈脾) 태자삼(太子蔘) 황기(黃?) 감초(甘草) 등을 기본적으로 사용한다. 치험(治驗): (1) 천식의 실증(實證)은 치료를 한 뒤 완화단계에 접어들면서 허증(虛證)으로 전화되는데, 허천(虛喘)도 역시 천식이니 만큼 단순히 보법(補法)만 사용하여서는 안된다. 시종일관 천식치료에는 척담화어(滌痰化瘀)의 원칙을 지켜야 한다. 폐내(肺內)의 담어(痰瘀)가 철저하게 제거되어야 폐(肺)의 순환기능을 개선시켜 기도(氣道)의 염증을 신속히 흡수하고 치유할 수 있다. 이와 같은 치료과정은 약 3-6개월이 필요하다. 완화단계의 기간이 길어질수록 완치될 확률도 높아진다. (2) 급성기 천식은 폐(肺)를 다스려야 한다. 폐(肺)를 다스리는 방법에는 선폐(宣肺), 청폐(淸肺), 온폐(溫肺), 윤폐(潤肺) 및 척담거어(滌痰祛瘀) 등이 있다. (3) 증상이 완화되면 신(腎)을 다스린다. 천식은 신허(腎虛)가 근본적인 원인이므로 완화단계에서 심지어 발작기에도 보신제(補腎劑)를 추가하여 사용하면 배의 효과를 얻을

소아 재발성 삼출성 中耳炎에서 Tryptase, Superoxide Dismutase 발현에 관한 연구

도금록,황우준,장철호,박은정,진경선,조재운,강성도,이현 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5

The purpose of this expriment is to evaluate the antiallergic and antioxidant effects of Kamihyunggyeyungyo-tang on recurrent otitis media with effusion. The author measured the level of Tryptase and Superoxide dismutase from 22 patients of middle ear effusion. Among the 22 patients, 11 patients had been treatad by antibiotics, and the other 11 patients had been treated by Kamihyunggyeyungyo-tang of pediatric recurrent otitis media with effusion(ROME) using Western blot method. In results, the level of tryptase of middle ear effusion and adenoid tissue, the level of Superoxide dismutase of middle ear effusion and adenoid in Kamihyiunggyeyungyo-tang group was significant than that of antibiotics group. Kamihyunggyeyungyo-tang is considered to be appropriate for the treatment of recurrent otitis with effusion and also it has better antiallergic and antioxidant effect than antibiotics.

Effect of temperature on the growth rate of cyst nematodes in summer chinese cabbage in Gangwon province

Se Jeong Hwang,Seul Gi Park,Sun Ju Lee,Mi Ran Hwang,Geum Ok Lee,Heon Seop Won,Kyung Rin Bang,Gwang Jae Lee,An Soo Lee,Ki Sun Kim,Tae Seong Jeong 한국응용곤충학회 2021 한국응용곤충학회 학술대회논문집 Vol.2021 No.04

Selection of best alternate application combination for controlling white fly to tomato

Se Jeong Hwang,Mi Ran Hwang,Seul Gi Park,Geum Ok Lee,Heon Seop Won,Jae Hong Lee,An Soo Lee,Tae Seong Jeong,Dae Gi Hong 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10